KEGG   ENZYME: 4.99.1.10
Entry
EC 4.99.1.10                Enzyme                                 

Name
magnesium dechelatase;
SGR (gene name);
SGRL (gene name);
Mg-dechelatase
Class
Lyases;
Other lyases;
Sole sub-subclass for lyases that do not belong in the other subclasses
Sysname
chlorophyll a magnesium lyase
Reaction(IUBMB)
(1) chlorophyll a + 2 H+ = pheophytin a + Mg2+ [RN:R08584];
(2) chlorophyllide a + 2 H+ = pheophorbide a + Mg2+ [RN:R09033]
Reaction(KEGG)
R08584 R09033
Substrate
chlorophyll a [CPD:C05306];
H+ [CPD:C00080];
chlorophyllide a [CPD:C02139]
Product
pheophytin a [CPD:C05797];
Mg2+ [CPD:C00305];
pheophorbide a [CPD:C18021]
Comment
Inhibited by Ca2+, Mg2+ and especially Hg2+. SGR has very low activity with chlorophyllide a and none with chlorophyll b. It acts on chlorophyll a both in its free form and in protein complex. SGRL, on the other hand, is more active with chlorophyllide a than with chlorophyll a. The magnesium formed is scavenged by MCS (metal-chelating substance).
History
EC 4.99.1.10 created 2017
Pathway
ec00860  Porphyrin and chlorophyll metabolism
ec01110  Biosynthesis of secondary metabolites
Orthology
K22013  magnesium dechelatase
Genes
ATH: AT1G44000 AT4G11910 AT4G11911 AT4G22920(NYE1)
ALY: 110227542 9303816 9310800 9330036
CRB: 17880941 17882664 17898091
CSAT: 104707485 104707486 104718013 104718062 104718066 104722741 104731201 104737198 104737199 104742156 104757939 104777551
EUS: EUTSA_v10011724mg EUTSA_v10025991mg EUTSA_v10028881mg EUTSA_v10029210mg
BRP: 103834271 103847714 103858612 103860469(NYE1) 103861346
BNA: 106368456 106368457 106375753 106375754 106379154 106388569 106405908 106411225 106411238 106416069 106438786 106440469 106440765 111197916
BOE: 106296745 106306852 106311628 106325928 106332354 106332703
RSZ: 108810996 108820491 108820492 108820494 108843007 108844784 108846175 108849663 108849678
THJ: 104801615 104813011 104814157
LJA: Lj1g3v0900940.1(Lj1g3v0900940.1) Lj2g3v2003030.1(Lj2g3v2003030.1) Lj4g3v1287250.1(Lj4g3v1287250.1) Lj4g3v1327600.1(Lj4g3v1327600.1)
OSA: 4347672
DOSA: Os04t0692600-01(Os04g0692600) Os09t0532000-01(Os09g0532000)
OBR: 102711183
ZMA: 100192013 606425(SGR1) 606426(SGR2)
AOF: 109821600
APRO: F751_5480
 » show all
Reference
1  [PMID:11204197]
  Authors
Tang L, Okazawa A, Fukusaki E, Kobayashi A
  Title
Removal of magnesium by Mg-dechelatase is a major step in the chlorophyll-degrading pathway in Ginkgo biloba in the process of autumnal tints.
  Journal
Z Naturforsch C 55:923-6 (2000)
DOI:10.1515/znc-2000-11-1213
Reference
2
  Authors
Costa, M.A., Civello, P.M., Chaves, A.R. and Martinez, G.A.
  Title
Characterization of Mg-dechelatase activity obtained from Fragaria x ananassa fruit.
  Journal
Plant Physiol Biochem 40:111-118 (2002)
Reference
3  [PMID:15154496]
  Authors
Wang T, Quisenberry SS, Ni X, Tolmay V
  Title
Enzymatic chlorophyll degradation in wheat near-isogenic lines elicited by cereal aphid (Homoptera: Aphididae) feeding.
  Journal
J Econ Entomol 97:661-7 (2004)
DOI:10.1603/0022-0493-97.2.661
Reference
4  [PMID:15890522]
  Authors
Suzuki T, Kunieda T, Murai F, Morioka S, Shioi Y
  Title
Mg-dechelation activity in radish cotyledons with artificial and native substrates, Mg-chlorophyllin a and chlorophyllide a.
  Journal
Plant Physiol Biochem 43:459-64 (2005)
DOI:10.1016/j.plaphy.2005.03.009
Reference
5
  Authors
Kunieda, T., Amano, T. and Shioi, Y.
  Title
Search for chlorophyll degradation enzyme, Mg-dechelatase, from extracts of Chenopodium album with native and artificial substrates.
  Journal
Plant Sci 169:177-183 (2005)
Reference
6  [PMID:27604697]
  Authors
Shimoda Y, Ito H, Tanaka A
  Title
Arabidopsis STAY-GREEN, Mendel's Green Cotyledon Gene, Encodes Magnesium-Dechelatase.
  Journal
Plant Cell tpc.00428.2016 (2016)
DOI:10.1105/tpc.16.00428
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.99.1.10
IUBMB Enzyme Nomenclature: 4.99.1.10
ExPASy - ENZYME nomenclature database: 4.99.1.10
BRENDA, the Enzyme Database: 4.99.1.10

DBGET integrated database retrieval system