KEGG   ENZYME: 4.99.1.12
Entry
EC 4.99.1.12                Enzyme                                 

Name
pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel chelatase;
LarC;
P2TMN nickel chelatase
Class
Lyases;
Other lyases;
Sole sub-subclass for lyases that do not belong in the other subclasses
Sysname
Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide nickel-lyase (pyridinium-3,5-bisthiocarboxylate-mononucleotide-forming)
Reaction(IUBMB)
Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide = pyridinium-3,5-bisthiocarboxylate mononucleotide + Ni2+ [RN:R11921]
Reaction(KEGG)
R11921
Substrate
Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide [CPD:C21769]
Product
pyridinium-3,5-bisthiocarboxylate mononucleotide [CPD:C21767];
Ni2+ [CPD:C19609]
Comment
This enzyme, found in Lactobacillus plantarum, is involved in the biosynthesis of a nickel-pincer cofactor. It catalyses the insertion of Ni2+ into the cofactor forming a covalent bond between a carbon atom and the nickel atom.
History
EC 4.99.1.12 created 2017
Orthology
K09121  pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel chelatase
Genes
TCI: A7K98_01705
VSH: BSZ05_17075
EMO: DM558_01535
CATE: C2869_06440
THIP: N838_08695
GAI: IMCC3135_06235
KUS: B9G99_09390
KUY: FY550_02745(larC)
BUM: AXG89_23670 AXG89_29020
SUTT: SUTMEG_10630
WSU: WS0139
CGRA: CGRAC_1984
CSPF: CSF_0737
CMUC: CMCT_0036
CSHO: CSHOW_0340
SDL: Sdel_1278
SMUL: SMUL_1677
SHAL: SHALO_1632
SULJ: SJPD1_1639
SULT: FA592_00730(larC)
GSU: GSU0141
GME: Gmet_0194
GUR: Gura_0215
GLO: Glov_3163
GBM: Gbem_3626
GEO: Geob_1111
GEM: GM21_3731
GEB: GM18_4084
PCA: Pcar_2401
PPD: Ppro_0626
DEU: DBW_0926
DVU: DVU2772
DVL: Dvul_0537
DVM: DvMF_0528
DDE: Dde_0766
DDS: Ddes_1915
DMA: DMR_16560
DGG: DGI_2650
DTR: RSDT_0249
DFL: DFE_2084
DCB: C3Y92_07470(larC)
DAS: Daes_0013
DPI: BN4_20237
PPRF: DPRO_1955
PSEL: GM415_09180(larC)
DBA: Dbac_0709
DRT: Dret_0715
DPS: DP0748
DOG: HP555_04990(larC)
DSF: UWK_00825
DOL: Dole_2215
DAL: Dalk_2602
DALK: DSCA_11600
DLI: dnl_37430(larC)
DMM: dnm_088040(larC)
ADE: Adeh_4313
MXA: MXAN_6689
AVM: JQX13_07600(larC)
SCL: sce2034
HOH: Hoch_2562
SAT: SYN_00476
SFU: Sfum_2531
DAX: FDQ92_06120(larC)
DBR: Deba_0902
BSED: DN745_16770(larC)
PCAY: FRD00_11150(larC)
KAI: K32_38500
SNO: Snov_3499
MET: M446_3799
MOR: MOC_1720
RBM: TEF_02780
MALG: MALG_04018
BLAS: BSY18_3647
MGM: Mmc1_1785
GKA: GK1963
GTN: GTNG_1862
HLI: HLI_15660
SER: SERP0253
SEP: SE0371
SEPP: SEB_00293
SEPS: DP17_1678
SSP: SSP0174
SPAS: STP1_1685
SARL: SAP2_03220
PMW: B2K_05175
PANC: E2636_15525(larC)
LPL: lp_0106(larC1) lp_0107(larC2)
LPT: zj316_0315(larC1) zj316_0316(larC2)
LFE: LAF_1731
LFR: LC40_1099
LFF: LBFF_1913
LMAL: LM596_00650(larC)
LBR: LVIS_1851
LSUA: H3M12_11155(larC)
LNN: F0161_06425(larC)
PPE: PEPE_1692
PPEN: T256_08325
PCE: PECL_1781
EFA: EF1827
EGV: EGCR1_11150(larC) EGCR1_12080(larC)
CAC: CA_C0774
CAE: SMB_G0790
CAY: CEA_G0785
CBO: CBO0103
CBA: CLB_0139
CBH: CLC_0151
CBY: CLM_0146
CBL: CLK_3278
CBB: CLD_0683
CBI: CLJ_B0141
CBF: CLI_0158
CBE: Cbei_0559
CBZ: Cbs_0559
CBEI: LF65_00620
CKL: CKL_0332
CKR: CKR_0284
CPAS: Clopa_0769
CBV: U729_1764
CTYK: CTK_C01040
CDY: F3K33_03235(larC)
CFER: D4Z93_06660(larC) D4Z93_07790(larC)
AMT: Amet_0481
CALE: FDN13_10165(larC)
SARJ: HH195_01555(larC)
CAZO: G3A45_06900(larC)
CTH: Cthe_0369
HSC: HVS_11375
CCE: Ccel_2204
RHER: EHE19_013565(larC)
CCEL: CCDG5_0276
RUM: CK1_01290
CAPR: EQM14_02370(larC)
OVA: OBV_12520
OBJ: EIO64_06790(larC)
BFI: CIY_29870
RIX: RO1_10590
RIM: ROI_27030
CCT: CC1_07270
ROB: CK5_09770
BLAB: EYS05_12425(larC)
CSO: CLS_11760
ACAC: EYQ97_14265(larC)
EHL: EHLA_1342
PXV: FXF36_05175(larC)
RTO: RTO_25340
LBW: C3V36_11980(larC)
PDC: CDIF630_00317(larC)
SLP: Slip_2374
SALQ: SYNTR_2029
DSY: DSY2209
DHD: Dhaf_3339
DAE: Dtox_3548
DAU: Daud_2227
SGY: Sgly_3310
HMO: HM1_1640
EMT: CPZ25_000635(larC) CPZ25_014830(larC)
ALKA: J0B03_03365(larC)
CTHM: CFE_2759
VPY: HZI73_08180(larC)
MDV: C5Q96_01225(larC)
AMIC: Ami3637_04845(larC)
BPRS: CK3_00540
MTA: Moth_2512
ATE: Athe_1635
TOC: Toce_0388
KME: H0A61_01718(larC)
VPR: Vpar_0985
STED: SPTER_16680(larC_1) SPTER_37050(larC_2)
AIN: Acin_0146
ERM: EYR00_11790(larC)
CGL: NCgl2383(Cgl2470)
CGB: cg2715
CGU: WA5_2383
CGT: cgR_2381
CGM: cgp_2715
CGJ: AR0_11850
CEF: CE2361
CSUR: N24_2544
SLE: sle_12230(sle_12230)
SNZ: DC008_00250(larC)
SBY: H7H31_31755(larC)
SERJ: SGUI_3203
ORN: DV701_00845(larC)
MPH: MLP_05640
MIK: FOE78_14105(larC)
RAIN: Rai3103_03785(larC)
NCA: Noca_0168
NDK: I601_1385
NSN: EXE58_07880(larC)
NANO: G5V58_23540(larC)
NMES: H9L09_09535(larC)
KFL: Kfla_0198
KQI: F1D05_21585(larC)
ACTW: F7P10_09285(larC)
NOW: GBF35_28955(larC)
GOB: Gobs_1561
MMAR: MODMU_0275
PDX: Psed_0535
PSEE: FRP1_11800
PAUT: Pdca_54880
ASE: ACPL_4836
ACTN: L083_6349
AFS: AFR_19490
ACTS: ACWT_4705
CAI: Caci_4915
SNA: Snas_6460
RXY: Rxyl_0176
RUB: GBA63_21295(larC)
AFO: Afer_0099
ATQ: GH723_17030(larC)
ELE: Elen_1214
AEQ: AEQU_1342
SYN: slr1411
SYZ: MYO_11560
SYY: SYNGTS_0157(slr1411)
SYT: SYNGTI_0157(slr1411)
SYS: SYNPCCN_0157(slr1411)
SYQ: SYNPCCP_0157(slr1411)
SYC: syc1752_c
SYW: SYNW1802
SYG: sync_2051
CYA: CYA_0950
CYB: CYB_0182
SYNR: KR49_12415
SYND: KR52_13645
SYNC: CB0101_05555(larC)
TEL: tll2296
THEC: FFX45_04920(larC)
PMA: Pro_0486
PMM: PMM0487
PMT: PMT_1291
PRC: EW14_0532
PRM: EW15_0584
AMR: AM1_5136
THEU: HPC62_06185(larC)
MAR: MAE_11470
MPK: VL20_2408
ENN: FRE64_10810(larC)
TER: Tery_0652
OXY: HCG48_24760(larC)
LFS: HFV01_23765(larC)
GVI: gll0542
GLJ: GKIL_4044
ANA: all0200
NSH: GXM_01853
NED: HUN01_30345(larC)
AVA: Ava_2692
NAZ: Aazo_4936
ANN: EH233_13765(larC)
CALH: IJ00_23035
DFS: HGD76_10550(larC)
CCUR: IAR63_14335(larC)
AFLO: HEQ12_18695(larC)
TOQ: HCG51_20905(larC)
CTHE: Chro_4850
CEO: ETSB_0087
RCA: Rcas_1165
TRO: trd_0315
ATM: ANT_23230
KBS: EPA93_42530(larC)
TTR: Tter_1404
CAA: Caka_1887
AMU: Amuc_1596
AGL: PYTT_2053
VBS: EGM51_07025(larC)
RBA: RB10177
PSL: Psta_2470
RUL: UC8_37960
AHEL: Q31a_23560
TTF: THTE_1572
PLM: Plim_2400
GIM: F1728_09230(larC)
FTJ: FTUN_1948
IPA: Isop_2077
SACI: Sinac_2353
VBC: C5Q97_11700(larC)
TPED: TPE_0826
ACA: ACP_0086
GRW: FTO74_05180(larC)
ABAS: ACPOL_5636
EDA: GWR55_06535(larC)
SUS: Acid_3354
PFER: IRI77_17670(larC)
EPO: Epro_1179
ETI: RSTT_617
RSD: TGRD_656
FUL: C4N20_13050(larC)
TLI: Tlie_1860
GBA: J421_3233
FOP: FNB79_14680(larC)
TMA: TM0964
TMW: THMA_0987
TMQ: THMB_0987
TMX: THMC_0987
TPT: Tpet_1783
TRQ: TRQ2_1826
TNA: CTN_1612
TNP: Tnap_1784
ASAC: ATHSA_1768
GTL: EP073_08930(larC)
DTU: Dtur_1544
NDE: NIDE0534
NJA: NSJP_1577
TAV: G4V39_00285(larC)
TMAI: FVE67_05540(larC)
NLI: G3M70_16595(larC)
MJA: MJ_1387
MAE: Maeo_0157
MVO: Mvol_1326
MTH: MTH_1109
METC: MTCT_1003
METE: tca_01065
METK: FVF72_06035(larC)
MTHM: FZP57_07180(larC)
MST: Msp_1170
MRU: mru_1890
MSI: Msm_0935
MEB: Abm4_0319
MMIL: sm9_1887
MEYE: TL18_08865
MOL: YLM1_1341
METH: MBMB1_1638
MFC: BRM9_0891
MCUB: MCBB_1945
MFV: Mfer_0384
MKA: MK1105
AFU: AF_2097
FPL: Ferp_2176
GAC: GACE_0061
GAH: GAH_00312
MBAR: MSBR2_2095
MBAK: MSBR3_2079
MAC: MA_0429
MMA: MM_1617
MMAC: MSMAC_3072
METM: MSMTP_2952
MTHR: MSTHT_2151
MTHE: MSTHC_1132
MFZ: AOB57_005140(larC)
MBU: Mbur_2265
MMH: Mmah_0803
MPY: Mpsy_0858
MZI: HWN40_04370(larC)
MTP: Mthe_1127
MHI: Mhar_1278
MHU: Mhun_2452
MEMA: MMAB1_1742
MBN: Mboo_2428
MPL: Mpal_0261
MEZ: Mtc_0230
RCI: RCIX692
HALR: EFA46_001515(larC)
HMA: rrnAC1529
HHI: HAH_2101
HALJ: G9465_15615(larC)
NMO: Nmlp_3816
HUT: Huta_0903
HTI: HTIA_0762
HALA: Hrd1104_08635(larC)
HMU: Hmuk_1782
HALZ: E5139_12030(larC)
HALL: LC1Hm_1079
HRR: HZS55_12455(larC)
HPEL: HZS54_16300(larC)
HLT: I7X12_01495(larC)
HWA: HQ_3176A
HWC: Hqrw_3719
HVO: HVO_2381
HME: HFX_2389
HALE: G3A49_15115(larC)
HAER: DU502_12990(larC)
HRA: EI982_17220(larC)
HALM: FCF25_03300(larC)
HLA: Hlac_1654
HALB: EKH57_02405(larC)
HEZZ: EO776_09090(larC)
HALQ: Hrr1229_013855(larC)
HSS: J7656_07360(larC)
HALG: HUG10_09000(larC)
HALU: HUG12_07280(larC)
HTU: Htur_2177
HALY: HYG82_07335(larC)
HSAL: JMJ58_18025(larC)
HLO: J0X27_14205(larC)
NMG: Nmag_0175
NVR: FEJ81_06180(larC)
SALI: L593_08740
MARC: AR505_1596
IAG: Igag_1707
NMR: Nmar_0339
NIW: Nisw_03210(larC)
NCT: NMSP_1365
NEV: NTE_02780
NCV: NCAV_1612
NBV: T478_0269
NDV: NDEV_0297
KCR: Kcr_0480
BARB: AOA66_0334
 » show all
Reference
1  [PMID:24710389]
  Authors
Desguin B, Goffin P, Viaene E, Kleerebezem M, Martin-Diaconescu V, Maroney MJ, Declercq JP, Soumillion P, Hols P
  Title
Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system.
  Journal
Nat Commun 5:3615 (2014)
DOI:10.1038/ncomms4615
  Sequence
Reference
2  [PMID:27114550]
  Authors
Desguin B, Soumillion P, Hols P, Hausinger RP
  Title
Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion.
  Journal
Proc Natl Acad Sci U S A 113:5598-603 (2016)
DOI:10.1073/pnas.1600486113
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.99.1.12
IUBMB Enzyme Nomenclature: 4.99.1.12
ExPASy - ENZYME nomenclature database: 4.99.1.12
BRENDA, the Enzyme Database: 4.99.1.12

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