Entry
Name
heparosan-N-sulfate-glucuronate 5-epimerase;
heparosan epimerase;
heparosan-N-sulfate-D-glucuronosyl 5-epimerase;
C-5 uronosyl epimerase;
polyglucuronate epimerase;
D-glucuronyl C-5 epimerase;
poly[(1,4)-beta-D-glucuronosyl-(1,4)-N-sulfo-alpha-D-glucosaminyl] glucurono-5-epimerase
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
BRITE hierarchy
Sysname
poly[(1->4)-beta-D-glucuronosyl-(1->4)-N-sulfo-alpha-D-glucosaminyl] glucurono-5-epimerase
Reaction(IUBMB)
Epimerization of D-glucuronate in heparosan-N-sulfate to L-iduronate. [RN:
R04389 ]
Reaction(KEGG)
Comment
The enzyme acts on D-glucosyluronate residues in N-sulfated heparosan polymers, converting them to L-iduronate, thus modifying the polymer to heparan-N-sulfate. The enzyme requires that at least the N-acetylglucosamine residue linked to C-4 of the substrate has been deacetylated and N-sulfated, and activity is highest with fully N-sulfated substrate. It does not act on glucuronate residues that are O-sulfated or are adjacent to N-acetylglucosamine residues that are O-sulfated at the 6 position. Thus the epimerization from D-glucuronate to L-iduronate occurs after N-sulfation of glucosamine residues but before O-sulfation. Not identical with EC
5.1.3.19 chondroitin-glucuronate 5-epimerase or with EC
5.1.3.36 , heparosan-glucuronate 5-epimerase.
History
EC 5.1.3.17 created 1984, modified 2015
Pathway
ec00534 Glycosaminoglycan biosynthesis - heparan sulfate / heparin
Orthology
K01793 heparosan-N-sulfate-glucuronate 5-epimerase
Genes
XLA : 108712794(glce.S) 432242(glce.L)
DRE : 100007670(glceb) 405776(glcea)
SRX : 107720792 107740438 107740877 107755093
SANH : 107663974 107670231 107673273 107700477
SGH : 107553083 107557703 107559985 107569710
CCAR : 109066693 109093126 109109568(glcea)
CAUA : 113043709 113052345 113079266 113106000
CGIB : 127962113 128014596 128016907
PTET : 122330363(glcea) 122348299 122349241(glceb)
LROH : 127156392(glcea) 127168418(glceb)
GRF : 141301930(glcea) 141331184(glceb)
OMC : 131534070(glcea) 131544352(glceb)
PPRM : 120475357(glcea) 120491029(glceb)
RKG : 130073707(glcea) 130082981(glceb)
MAMB : 125246813(glcea) 125264777(glceb)
CIDE : 127507476 127515223
CERY : 137015273(glcea) 137029803(glceb)
MASI : 127416399 127436102 127437561 127443782
TROS : 130551922(glcea) 130557793(glceb)
TDW : 130414305(glcea) 130427134(glceb)
MANU : 129433850(glcea) 129443782(glceb)
PDAB : 135749192(glceb) 135787789(glcea)
IPU : 108259276(glceb) 108274589(glcea)
IFU : 128602706(glceb) 128617895(glcea)
PHYP : 113529971 113540832
SMEO : 124379867(glceb) 124391984(glcea)
TFD : 113636763(glceb) 113653913(glcea)
TVC : 132838729(glceb) 132856931(glcea)
CGAR : 128512097(glcea) 128519023(glceb)
NGRA : 132875299(glceb) 132881715(glcea)
AMEX : 103040160(glcea) 103040217(glceb)
CMAO : 118811890(glcea) 118817488(glceb)
CHAR : 105892465(glcea) 105906560(glceb)
SPIL : 134094670(glcea) 134095794(glceb)
SASA : 106562601 106562708 106593990(glce)
STRU : 115166801 115203527
OCLA : 139398190 139411735
PSPA : 121294489 121299204
ARUT : 117427879 117429202
AAMU : 139950287 139950314
AFIL : 140167904 140168407
DSI : Dsimw501_GD10315(Dsim_GD10315)
AALB : 109397352 109420263
CFUM : 141428392 141428394(Hsepi)
CFEL : 113363902 113377896
LPOL : 106459105 106467792 106477692
CBR : CBG_18086(Cbr-hse-5)
BMY : BM_BM1737(Bma-hse-5)
CANU : 128188555 128188865
DPOL : 127867599 127867600
LAK : 106161783 106167628 106169258 106173383
CLJ : CLJU_c25590 CLJU_c26810
CAH : CAETHG_0628 CAETHG_0762
» show all
Taxonomy
Reference
Authors
Backstrom G, Hook M, Lindahl U, Feingold DS, Malmstrom A, Roden L, Jacobsson I.
Title
Biosynthesis of heparin. Assay and properties of the microsomal uronosyl C-5 epimerase.
Journal
J Biol Chem 254:2975-82 (1979)
Reference
Authors
Jacobsson I, Lindahl U, Jensen JW, Roden L, Prihar H, Feingold DS
Title
Biosynthesis of heparin. Substrate specificity of heparosan N-sulfate D-glucuronosyl 5-epimerase.
Journal
J Biol Chem 259:1056-63 (1984)
Reference
Authors
Hagner-McWhirter A, Hannesson HH, Campbell P, Westley J, Roden L, Lindahl U, Li JP
Title
Biosynthesis of heparin/heparan sulfate: kinetic studies of the glucuronyl C5-epimerase with N-sulfated derivatives of the Escherichia coli K5 capsular polysaccharide as substrates.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 5.1.3.17
ExPASy - ENZYME nomenclature database: 5.1.3.17