KEGG ENZYME: 5.1.3.17

ENZYME: 5.1.3.17

Entry

EC 5.1.3.17                 Enzyme

Name

heparosan-N-sulfate-glucuronate 5-epimerase;
heparosan epimerase;
heparosan-N-sulfate-D-glucuronosyl 5-epimerase;
C-5 uronosyl epimerase;
polyglucuronate epimerase;
D-glucuronyl C-5 epimerase;
poly[(1,4)-beta-D-glucuronosyl-(1,4)-N-sulfo-alpha-D-glucosaminyl] glucurono-5-epimerase

Class

Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives

Sysname

poly[(1->4)-beta-D-glucuronosyl-(1->4)-N-sulfo-alpha-D-glucosaminyl] glucurono-5-epimerase

Reaction(IUBMB)

Epimerization of D-glucuronate in heparosan-N-sulfate to L-iduronate. [RN:R04389]

Reaction(KEGG)

R04389

Comment

The enzyme acts on D-glucosyluronate residues in N-sulfated heparosan polymers, converting them to L-iduronate, thus modifying the polymer to heparan-N-sulfate. The enzyme requires that at least the N-acetylglucosamine residue linked to C-4 of the substrate has been deacetylated and N-sulfated, and activity is highest with fully N-sulfated substrate. It does not act on glucuronate residues that are O-sulfated or are adjacent to N-acetylglucosamine residues that are O-sulfated at the 6 position. Thus the epimerization from D-glucuronate to L-iduronate occurs after N-sulfation of glucosamine residues but before O-sulfation. Not identical with EC 5.1.3.19 chondroitin-glucuronate 5-epimerase or with EC 5.1.3.36, heparosan-glucuronate 5-epimerase.

History

EC 5.1.3.17 created 1984, modified 2015

Pathway

ec00534

Glycosaminoglycan biosynthesis - heparan sulfate / heparin

ec01100

Metabolic pathways

Orthology

K01793

heparosan-N-sulfate-glucuronate 5-epimerase

Genes

HSA:	26035(GLCE)

PTR:	453542(GLCE)

PPS:	100975106(GLCE)

GGO:	101148037(GLCE)

PON:	100457760(GLCE)

NLE:	100592444(GLCE)

MCC:	694999(GLCE)

MCF:	102130661(GLCE)

CSAB:

103245410(GLCE)

CATY:

105600958(GLCE)

PANU:

101025031(GLCE)

TGE:	112627967(GLCE)

RRO:	104662604(GLCE)

RBB:	108514748(GLCE)

TFN:	117070431(GLCE)

PTEH:

111548375(GLCE)

CJC:	100402503(GLCE)

SBQ:	101034113(GLCE)

CSYR:

103273821(GLCE)

MMUR:

105873420(GLCE)

LCAT:

123623484(GLCE)

OGA:	100966636(GLCE)

MMU:	93683(Glce)

MCAL:

110301842(Glce)

MPAH:

110327461(Glce)

RNO:	363073(Glce)

MCOC:

116073962(Glce)

MUN:	110550219(Glce)

CGE:	100754874(Glce)

MAUA:

101836212(Glce)

PLEU:

114691670(Glce)

MORG:

121466157(Glce)

AAMP:

119810193(Glce)

NGI:	103736825(Glce)

HGL:	101703064(Glce)

CPOC:

100733856(Glce)

CCAN:

109698168(Glce)

DORD:

105982523(Glce)

DSP:	122122999(Glce)

NCAR:

124977563

OCU:	100341828(GLCE)

OPI:	101529827(GLCE)

TUP:	102502937(GLCE)

CFA:	487622(GLCE)

CLUD:

112675683(GLCE)

VVP:	112931528(GLCE)

VLG:	121484132(GLCE)

AML:	100478227(GLCE)

UMR:	103679039(GLCE)

UAH:	113240917(GLCE)

UAR:	123800773(GLCE)

ELK:

111153319

LLV:

125104837

MPUF:

101670150(GLCE)

ORO:	101386831(GLCE)

EJU:	114206527(GLCE)

ZCA:	113909686(GLCE)

MLX:	118003606(GLCE)

FCA:	101095983(GLCE)

PYU:	121041910(GLCE)

PBG:	122467608(GLCE)

PTG:	102952073(GLCE)

PPAD:

109262774(GLCE)

AJU:	106980561(GLCE)

HHV:	120221934(GLCE)

BTA:	281195(GLCE)

BOM:	102274550(GLCE)

BIU:	109564721(GLCE)

BBUB:

102405908(GLCE)

CHX:	102172900(GLCE)

OAS:	101106592(GLCE)

ODA:	120852503 120860868

CCAD:

122443089(GLCE)

SSC:	100154444(GLCE)

CFR:	102504983(GLCE)

CBAI:

105069233(GLCE)

CDK:	105087308(GLCE)

VPC:	102534038(GLCE)

BACU:

103008441(GLCE)

LVE:	103080354(GLCE)

OOR:	101271197(GLCE)

DLE:	111177767(GLCE)

PCAD:

102990323(GLCE)

PSIU:

116749662(GLCE)

ECB:	100052581(GLCE)

EPZ:	103543875(GLCE)

EAI:	106835661(GLCE)

MYB:	102262970(GLCE)

MYD:	102752823(GLCE)

MMYO:

118654570(GLCE)

MLF:	102423949(GLCE)

MNA:	107525844(GLCE)

PKL:	118718777(GLCE)

HAI:	109385253(GLCE)

DRO:	112311999(GLCE)

SHON:

118992362(GLCE)

AJM:	119056987(GLCE)

PDIC:

114491549(GLCE)

PHAS:

123808190(GLCE)

MMF:	118617890(GLCE)

RFQ:	117023538(GLCE)

PALE:

102891481(GLCE)

PGIG:

120604461(GLCE)

PVP:	105309629(GLCE)

RAY:	107511425(GLCE)

MJV:	108397133(GLCE)

TOD:	119238797(GLCE)

SARA:

101558339(GLCE)

LAV:	100673589(GLCE)

TMU:

101347909

DNM:	101444954(GLCE)

MDO:	100019166(GLCE)

GAS:	123236126(GLCE)

SHR:	100925822(GLCE)

PCW:	110205598(GLCE)

OAA:	100085653(GLCE)

GGA:	415566(GLCE)

PCOC:

116225491(GLCE)

MGP:	100546915(GLCE)

CJO:	107318962(GLCE)

NMEL:

110403606(GLCE)

APLA:

101789857(GLCE)

ACYG:

106043673(GLCE)

AFUL:

116493554(GLCE)

LSR:	110477573(GLCE)

SCAN:

103816259(GLCE)

PMOA:

120508939(GLCE)

OTC:	121343512(GLCE)

PRUF:

121354536(GLCE)

GFR:	102034065(GLCE)

FAB:	101818992(GLCE)

PHI:	102113112(GLCE)

PMAJ:

107209523(GLCE)

CCAE:

111933852(GLCE)

CBRC:

103619816(GLCE)

ETL:	114070300(GLCE)

ZAB:	106630389(GLCE)

FPG:	101915406(GLCE)

FCH:	102054462(GLCE)

CLV:	102093041(GLCE)

EGZ:	104133817(GLCE)

NNI:	104017037(GLCE)

PLET:

104613898(GLCE)

PCRI:

104024222(GLCE)

ACUN:

113484044(GLCE)

TALA:

104362314(GLCE)

PADL:

103914742(GLCE)

ACHC:

115341823(GLCE)

HALD:

104325661(GLCE)

CCRI:

104161014(GLCE)

CSTI:

104559768(GLCE)

EHS:	104512065(GLCE)

FGA:	104081776(GLCE)

GSTE:

104259531(GLCE)

LDI:

104340123

MNB:	103774713(GLCE)

AAM:

106495389

AROW:

112963593(GLCE)

NPD:	112948346(GLCE)

DNE:	112989520(GLCE)

ASN:	102386367(GLCE)

AMJ:	102568999(GLCE)

CPOO:

109309218(GLCE)

GGN:	109288409(GLCE)

PSS:	102453691(GLCE)

CMY:	114019430(GLCE)

CPIC:

101943859(GLCE)

TST:	117883528(GLCE)

CABI:

116815733(GLCE)

MRV:	120373550(GLCE)

ACS:	100556549(glce)

PVT:	110086904(GLCE)

SUND:

121933228(GLCE)

PBI:	103064301(GLCE)

PMUR:

107283920(GLCE)

TSR:	106540160(GLCE)

PGUT:

117676200(GLCE)

VKO:	123030701(GLCE)

PMUA:

114584097(GLCE)

ZVI:	118092529(GLCE)

GJA:	107116570(GLCE)

STOW:

125424828(GLCE)

XLA:	108712794(glce.S) 432242(glce.L)

XTR:	100489303(glce)

NPR:	108796035(GLCE)

RTEM:

120923836

BBUF:

120986043(GLCE)

BGAR:

122927478(GLCE)

DRE:	100007670(glceb) 405776(glcea)

SRX:	107720792 107740438 107740877 107755093

SANH:

107663974 107670231 107673273 107700477

SGH:	107553083 107557703 107559985 107569710

CCAR:

109050896 109066693 109093126 109109568

CAUA:

113043709 113052345 113079266 113106000

PPRM:

120475357(glcea) 120491029(glceb)

MAMB:

125246813(glcea) 125264777(glceb)

IPU:	108259276(glceb) 108274589(glcea)

PHYP:

113529971 113540832

SMEO:

124379867(glceb) 124391984(glcea)

TFD:	113636763(glceb) 113653913(glcea)

AMEX:

103040160 103040217

EEE:	113572322 113584499

TRU:	101074194(glce)

TNG:	GSTEN00018120G001

LCO:	104920418(glce)

NCC:	104944436(glce)

CGOB:

115005637(glce)

ELY:	117253633(glceb)

EFO:	125899220(glceb)

PLEP:

121946907(glceb)

SLUC:

116043452(glceb)

ECRA:

117950179(glceb)

PFLV:

114556531(glce)

GAT:	120812554(glceb)

PPUG:

119198574(glceb)

MSAM:

119890080(glceb)

CUD:	121507882(glceb)

ALAT:

119018323(glceb)

MZE:	101468415(glce)

ONL:	100689967(glce)

OAU:	116323950(glceb)

OLA:	101168104(glce)

OML:	112160751(glceb)

XMA:	102220367(glce)

XCO:	114142896(glce)

XHE:	116718446(glce)

PRET:

103462118(glce)

PFOR:

103146186(glce)

PLAI:

106964982(glce)

PMEI:

106930363(glce)

GAF:	122841808(glceb)

CVG:	107104897(glce)

CTUL:

119776853(glceb)

GMU:	124867026(glceb)

NFU:	107381748(glce)

KMR:	108234798(glceb)

ALIM:

106512969(glce)

NWH:	119424012(glceb)

AOCE:

111564420(glce)

MCEP:

125005684(glceb)

CSEM:

103398539(glce)

POV:	109637279(glce)

SSEN:

122772339(glceb)

HHIP:

117756084(glceb)

HSP:	118109205(glceb)

LCF:	108887864(glce)

SDU:	111223714(glce)

SLAL:

111670127(glce)

XGL:	120797172(glceb)

HCQ:	109514358(glce)

BPEC:

110174517(glce)

MALB:

109963362(glce)

BSPL:

114852278(glceb)

SASA:

106562601 106562708 106593990(glce)

OTW:	112264250 121845510

OMY:	110506198 110526997

OGO:	124035485(glceb)

SALP:

111980622(glce)

SNH:	120049768(glceb)

CCLU:

121548171(glceb)

ELS:	105013975(glce)

SFM:	108934585(glce)

PKI:	111845625(glce)

AANG:

118215235

LOC:	102694794(glce)

PSPA:

121294489 121299204

ARUT:

117427879 117429202

LCM:	102357523(GLCE)

CMK:

103190379

RTP:	109935199(glce)

BFO:

118429968

BBEL:

109471711

CIN:	100181100 113473995

SCLV:

120335536

SPU:

115918110

APLC:

110979098

SKO:

100370244

DME:	Dmel_CG3194(Hsepi)

DER:

6541647

DSE:

6607870

DSI:	Dsimw501_GD10315(Dsim_GD10315)

DYA:	Dyak_GE24632

DAN:

6496639

DSR:

110189521

DPO:

4803422

DPE:

6591115

DMN:	108159497 117190075

DWI:

6652210

DGR:

6559652

DMO:	Dmoj_GI20241

DAZ:

108616232

DNV:

108654278

DHE:

111597636

DVI:

6626477

CCAT:

101459521

BOD:

106626692

MDE:

101895364

SCAC:

106091743

LCQ:

111679193

LSQ:	119606790 119614316

HIS:

119649614

AGA:	AgaP_AGAP005699

ACOZ:

120949916

AARA:

120894259

ASTE:

118510552

AAG:

5565650

AALB:

109397352 109420263

CQU:	CpipJ_CPIJ014213

CPII:

120415752

CNS:

116352573

BCOO:

119081361

AME:

410118

ACER:

107994528

ALAB:

122719649

BIM:

100746913

BBIF:

117211829

BVK:

117231548

BVAN:

117166639

BTER:

100648289

BPYO:

122572307

CCAL:

108625516

OBB:

114873294

MGEN:

117225220

NMEA:

116425382

CGIG:

122397182

SOC:

105205016

MPHA:

105839326

AEC:

105152283

ACEP:

105621248

PBAR:

105428344

VEM:

105569445

HST:

105180547

DQU:

106743638

CFO:

105252887

FEX:

115245702

LHU:

105667459

PGC:

109856517

OBO:

105275234

PCF:

106785179

PFUC:

122525806

VPS:

122633444

NVI:

100123867

CSOL:

105359880

TPRE:

106654805

MDL:

103572179

CGLO:

123265683

FAS:

105271878

DAM:

107037394

AGIF:

122848386

CCIN:

107269276

TCA:

663342

DPA:

109539260

SOY:

115890532

ATD:

109604348

CSET:

123314239

AGB:

108914173

LDC:

111501961

NVL:

108565207

APLN:

108733612

PPYR:

116175551

OTU:

111415151

BMOR:

101736603

BMAN:

114251952

MSEX:

115451466

DPL:	KGM_205946

BANY:

112052728

PMAC:

106718979

PPOT:

106105194

PXU:

106128471

PRAP:

110999574

ZCE:

119835154

HAW:

110374867

HZE:

124631208

TNL:

113504444

SLIU:

111351881

OFU:

114351332

PXY:

105382951

API:

100164972

DNX:

107166129

AGS:

114124434

RMD:	113555611 113556575

BTAB:

109035995

DCI:

103509657

CLEC:

106670169

HHAL:

106680682

NLU:

111045855

PHU:	Phum_PHUM045460

FOC:

113205627

TPAL:

117649391

ZNE:

110840150

CSEC:

111862967

FCD:

110862144

DPX:	DAPPUDRAFT_325820

DMK:

116919414

PVM:

113806085

PJA:

122266525

PCHN:

125032807

HAME:

121861192

PCLA:

123762054

PTRU:

123512008

HAZT:

108678715

EAF:

111700314

LSM:

121117465

PPOI:

119109679

ISC:	IscW_ISCW009723

DSV:

119446560

RSAN:

119389727

RMP:

119177059

VDE:

111253483

VJA:

111264172

TUT:

107372174

DPTE:

113793709

DFR:

124494060

CSCU:

111642384

PTEP:

107446424

SDM:

118202796

CEL:	CELE_B0285.5(hse-5)

CBR:	CBG_18086(Cbr-hse-5)

BMY:	BM_BM1737(Bma-hse-5)

LOA:	LOAG_05488

NAI:	NECAME_11020

TSP:

Tsp_05727

HRO:	HELRODRAFT_142574

LGI:	LOTGIDRAFT_123168

PCAN:

112556648

BGT:	106071274 106078711

GAE:

121390640

HRF:

124149284

HRJ:

124280958

CRG:	105324867 105329756

MYI:

110455274

PMAX:

117344629

MMER:

123526242

OBI:

106876518

OSN:

115227227

LAK:	106161783 106167628 106169258 106173383

SMM:	Smp_126320

SHX:	MS3_0013895

OVI:	T265_06313

EGL:

EGR_02731

NVE:

5520075

EPA:

110241410

ATEN:

116299102

ADF:

107346476

AMIL:

114975962

PDAM:

113672224

SPIS:

111329368

DGT:

114518014

XEN:

124444471

HMG:

100207938

TAD:	TRIADDRAFT_27335

PTT:	VY86_07540

BMAR:

HF888_03430

KSD:	KS2013_1451

CHA:	CHAB381_1476

AANA:

AANAER_2044

NAM:

NAMH_1657

NAP:	C3L23_08545

GSB:	GSUB_15310

VNE:	CFK40_05110

LAO:	AOX59_11990

AQT:	FN924_03240

CKL:	CKL_1010 CKL_1095

CKR:	CKR_0914 CKR_0997

CLJ:	CLJU_c25590 CLJU_c26810

CAH:	CAETHG_0628 CAETHG_0762

CSQ:

CSCA_4800

CDRK:

B9W14_20875

RCH:

RUM_09550

BPRO:

PMF13cell1_00168

DMT:	DESME_14470

TTE:

TTE0659

NOW:	GBF35_13825

HHG:	XM38_017590

RUL:

UC8_39190

ABA:	Acid345_3516

AMO:	Anamo_1192

BUY:	D8S85_14480

BVIR:

I6J59_01675

SCN:	Solca_3918

FUV:	JR347_05040

MYR:	MYRA21_1118

MPW:

MPR_3643

MOD:	AS202_05235

FLU:	CHH17_10220

IAL:

IALB_2082

MSJ:	MSSAC_1842

MSW:	MSSIT_2435

» show all

Reference

1 [PMID:107165]

Authors

Backstrom G, Hook M, Lindahl U, Feingold DS, Malmstrom A, Roden L, Jacobsson I.

Title

Biosynthesis of heparin. Assay and properties of the microsomal uronosyl C-5 epimerase.

Journal

J Biol Chem 254:2975-82 (1979)

Reference

2 [PMID:6420398]

Authors

Jacobsson I, Lindahl U, Jensen JW, Roden L, Prihar H, Feingold DS

Title

Biosynthesis of heparin. Substrate specificity of heparosan N-sulfate D-glucuronosyl 5-epimerase.

Journal

J Biol Chem 259:1056-63 (1984)

Reference

3 [PMID:10642607]

Authors

Hagner-McWhirter A, Hannesson HH, Campbell P, Westley J, Roden L, Lindahl U, Li JP

Title

Biosynthesis of heparin/heparan sulfate: kinetic studies of the glucuronyl C5-epimerase with N-sulfated derivatives of the Escherichia coli K5 capsular polysaccharide as substrates.

Journal

Glycobiology 10:159-71 (2000)
DOI:10.1093/glycob/10.2.159

Other DBs

ExplorEnz - The Enzyme Database:

5.1.3.17

IUBMB Enzyme Nomenclature:

5.1.3.17

ExPASy - ENZYME nomenclature database:

5.1.3.17

BRENDA, the Enzyme Database:

5.1.3.17

CAS:	112567-86-9

DBGET integrated database retrieval system