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Entry
EC 5.1.3.30                 Enzyme                                 

Name
D-psicose 3-epimerase;
D-allulose 3-epimerase;
DPEase (ambiguous)
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
BRITE hierarchy
Sysname
D-psicose 3-epimerase
Reaction(IUBMB)
D-psicose = D-fructose [RN:R10817]
Reaction(KEGG)
Substrate
D-psicose [CPD:C06468]
Product
D-fructose [CPD:C00095]
Comment
The enzyme is highly specific for D-psicose and shows very low activity with D-tagatose (cf. EC 5.1.3.31, D-tagatose 3-epimerase). The enzyme from the bacterium Clostridium scindens requires Mn2+ [1], whereas the enzymes from the bacteria Clostridium cellulolyticum [2,5], Clostridium sp. BNL1100 [3], and Clostridium bolteae [4] require Co2+ as optimum cofactor. The enzyme from Ruminococcus sp. [6] is not dependent on the presence of metal ions, but its activity is enhanced by Mn2+.
History
EC 5.1.3.30 created 2014
Orthology
K18910  D-psicose/D-tagatose/L-ribulose 3-epimerase
Genes
VNI: VIBNI_B2134
VSH: BSZ05_18720
CEB: B0D95_16695
CEK: D0B88_14095
HJA: BST95_04425 BST95_12590
HALC: EY643_09855 EY643_18065
MMT: Metme_2524
MDH: AYM39_05640
MKO: MKLM6_1146
HOL: HORIV_69060
BCEN: DM39_5554
BMU: Bmul_3563
BMK: DM80_4951
BSTG: WT74_30375
BGU: KS03_4299
BGO: BM43_5843
VEI: Veis_3271
DAC: Daci_0782
ATU: Atu4748 Atu4750(dpe)
MMED: Mame_03157
HDI: HDIA_0684
RSP: RSP_3671
RDE: RD1_3873
DSH: Dshi_0533
CLS: CXIVA_15900(IolE)
CCE: Ccel_0941
ROB: CK5_09670
SALB: XNR_0898
SBH: SBI_01788
AAU: AAur_1891
SERJ: SGUI_0940
BLIN: BLSMQ_0068
MPH: MLP_04780
NDA: Ndas_4059
NAL: B005_1215
NML: Namu_1536
AMYY: YIM_38700
ACTI: UA75_21195
ACAD: UA74_20700
SNA: Snas_4652
SYY: SYNGTS_1069(sll1304)
SYT: SYNGTI_1069(sll1304)
SYS: SYNPCCN_1068(sll1304)
SYQ: SYNPCCP_1068(sll1304)
CAA: Caka_0707
RBA: RB11728
PSL: Psta_0700
RUL: UC8_02530
TTF: THTE_3776
PLM: Plim_0391
IPA: Isop_0568
SACI: Sinac_5698
SLI: Slin_0509
FAE: FAES_1761
FJO: Fjoh_4045
MARM: YQ22_00835
 » show all
Taxonomy
Reference
1  [PMID:21663329]
  Authors
Mu W, Chu F, Xing Q, Yu S, Zhou L, Jiang B
  Title
Cloning, expression, and characterization of a D-psicose 3-epimerase from Clostridium cellulolyticum H10.
  Journal
J Agric Food Chem 59:7785-92 (2011)
DOI:10.1021/jf201356q
  Sequence
[cce:Ccel_0941]
Reference
2  [PMID:22426981]
  Authors
Chan HC, Zhu Y, Hu Y, Ko TP, Huang CH, Ren F, Chen CC, Ma Y, Guo RT, Sun Y
  Title
Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars.
  Journal
Protein Cell 3:123-31 (2012)
DOI:10.1007/s13238-012-2026-5
  Sequence
[cce:Ccel_0941]
Reference
3  [PMID:22760176]
  Authors
Zhu Y, Men Y, Bai W, Li X, Zhang L, Sun Y, Ma Y
  Title
Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production.
  Journal
Biotechnol Lett 34:1901-6 (2012)
DOI:10.1007/s10529-012-0986-4
Reference
4  [PMID:23646168]
  Authors
Zhang W, Fang D, Xing Q, Zhou L, Jiang B, Mu W
  Title
Characterization of a novel metal-dependent D-psicose 3-epimerase from Clostridium scindens 35704.
  Journal
PLoS One 8:e62987 (2013)
DOI:10.1371/journal.pone.0062987
Reference
5  [PMID:23660703]
  Authors
Mu W, Zhang W, Fang D, Zhou L, Jiang B, Zhang T
  Title
Characterization of a D-psicose-producing enzyme, D-psicose 3-epimerase, from Clostridium sp.
  Journal
Biotechnol Lett 35:1481-6 (2013)
DOI:10.1007/s10529-013-1230-6
  Sequence
Reference
6  [PMID:23644747]
  Authors
Jia M, Mu W, Chu F, Zhang X, Jiang B, Zhou LL, Zhang T
  Title
A D-psicose 3-epimerase with neutral pH optimum from Clostridium bolteae for D-psicose production: cloning, expression, purification, and characterization.
  Journal
Appl Microbiol Biotechnol 98:717-25 (2014)
DOI:10.1007/s00253-013-4924-8
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.1.3.30
IUBMB Enzyme Nomenclature: 5.1.3.30
ExPASy - ENZYME nomenclature database: 5.1.3.30
BRENDA, the Enzyme Database: 5.1.3.30

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