KEGG ENZYME: 5.1.3.37

ENZYME: 5.1.3.37

Entry

EC 5.1.3.37                 Enzyme

Name

mannuronan 5-epimerase;
algG (gene name);
alginate epimerase;
C5-mannuronan epimerase;
mannuronan C-5-epimerase

Class

Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives

Sysname

[mannuronan]-beta-D-mannuronate 5-epimerase

Reaction(IUBMB)

[mannuronan]-beta-D-mannuronate = [alginate]-alpha-L-guluronate

Reaction(KEGG)

(other) R08693

Substrate

[mannuronan]-beta-D-mannuronate

Product

[alginate]-alpha-L-guluronate

Comment

The enzyme epimerizes the C-5 bond in some beta-D-mannuronate residues in mannuronan, converting them to alpha-L-guluronate residues, and thus modifying the mannuronan into alginate. It is found in brown algae and alginate-producing bacterial species from the Pseudomonas and Azotobacter genera.

History

EC 5.1.3.37 created 2015

Pathway

ec00051

Fructose and mannose metabolism

ec01100

Metabolic pathways

Orthology

K01795

mannuronan 5-epimerase

Genes

PAE:	PA3545(algG)

PAEV:

N297_3668(algG)

PAEI:

N296_3668(algG)

PAU:	PA14_18500(algG)

PAP:	PSPA7_1600(algG)

PAG:	PLES_14881(algG)

PAF:	PAM18_1441(algG)

PNC:	NCGM2_4670(algG)

PAEB:

NCGM1900_2739(algG)

PDK:	PADK2_06640

PSG:	G655_07075

PRP:	M062_18780

PAEP:

PA1S_07695

PAER:

PA1R_gp1363

PAEM:

U769_07170

PAEL:

T223_07410

PAES:

SCV20265_1518

PAEU:

BN889_03916(algG)

PAEG:

AI22_26210

PAEC:

M802_3665(algG)

PAEO:

M801_3533(algG)

PMY:	Pmen_1026 Pmen_1667

PMK:

MDS_1077

PRE:	PCA10_11830(algG) PCA10_38180(algG)

PFUW:

KF707C_11120

PALC:

A0T30_03860 A0T30_11830

PCQ:	PcP3B5_50730(algG)

PMUI:

G4G71_08950

PNT:	G5B91_26185

PPAA:

B7D75_07410

PPU:	PP_1283(algG)

PPF:

Pput_4442

PPG:	PputGB1_4566

PPW:	PputW619_0884

PPT:

PPS_4408

PPB:	PPUBIRD1_4278

PPI:

YSA_03103

PPX:

T1E_3163

PPUH:

B479_22175

PPUT:

L483_27260

PPUN:

PP4_07950(algG)

PPUD:

DW66_4799

PFV:	Psefu_3891

PMON:

X969_21710

PMOT:

X970_21345

PPJ:	RK21_00114

PMOL:

CLJ08_08405 CLJ08_22875

PALD:

LU682_024970(algG)

PIX:	RIN61_07145(algG)

PMOS:

O165_018585

PST:	PSPTO_1238(algG)

PSB:

Psyr_1058

PSYR:

N018_20380

PSP:	PSPPH_1113(algG)

PSAV:

PSA3335_09070

PAMG:

BKM19_008060

PCI:	PCH70_10080

PAVL:

BKM03_06995

PVD:	CFBP1590__4282(algG)

PCAB:

JGS08_19550(algG)

PCOF:

POR16_18950

PTRE:

I9H09_05065(algG)

PSYI:

MME58_05250(algG)

PAST:

N015_05960

PLIJ:

KQP88_04830(algG)

PFL:	PFL_1019(algG)

PPRC:

PFLCHA0_c10390(algG)

PPRO:

PPC_1057

PFE:	PSF113_4757

PFN:	HZ99_11940

PFO:	Pfl01_0955(algG)

PFS:	PFLU_0985(algG)

PFC:	PflA506_0965(algG)

PPZ:	H045_01665

PFB:

VO64_4066

PMAN:

OU5_2452

PTV:	AA957_09070

PCG:	AXG94_10000

PVR:	PverR02_05200

PAZO:

AYR47_12205

POI:	BOP93_04710

PRX:	HRH33_05780

PMED:

E3Z27_23145

PKR:	AYO71_07515

PMUD:

NCTC8068_04326(algG)

PTOL:

I7845_05020(algG)

PFW:	PF1751_v1c09430(algG)

PFF:	PFLUOLIPICF703415

PFX:	A7318_04265

PEN:	PSEEN3463 PSEEN4540(algG)

PBA:	PSEBR_a4595

PBC:	CD58_24330

PPUU:

PputUW4_04476(algG)

PDR:	H681_19450

PSV:	PVLB_04210 PVLB_15520

PSK:	U771_06100

PKC:	PKB_0963(algG)

PCH:	EY04_04495

PCZ:	PCL1606_50760

PCP:	JM49_25150

PCHP:

C4K32_1098

PFZ:	AV641_18535

PLQ:	AA042_15125

PALK:

PSAKL28_08690 PSAKL28_31910

PPV:	NJ69_13855

PSES:

PSCI_0726

PSEM:

TO66_05185

PSEC:

CCOS191_0907(algG) CCOS191_2019

PPSY:

AOC04_15755

PSOS:

POS17_1025

PFK:	PFAS1_06355

PANR:

A7J50_0987

PSET:

THL1_1263 THL1_3799

PSIL:

PMA3_24610

PYM:	AK972_0833

PKE:	DLD99_05135

PALL:

UYA_04860 UYA_15375

PUM:	HGP31_04950

POJ:	PtoMrB4_25890 PtoMrB4_46240(algG)

PGG:	FX982_00692

PGY:	AWU82_19105 AWU82_28980

PLAL:

FXN65_05530

PSEJ:

HNQ25_10115 HNQ25_19305

PVK:	EPZ47_24355

PEZ:	HWQ56_05025

PBZ:	GN234_23990

PKH:	JLK41_05195(algG)

PTY:	JWV26_01425 JWV26_15940(algG)

PATA:

JWU58_22775(algG)

PDW:	BV82_4695(algG)

PZE:	HU754_006245(algG)

PMOE:

HV782_005825(algG)

PCAM:

HNE05_19920

PSEP:

C4K39_1036

PPII:

QL104_05150(algG)

PVW:	HU752_027455(algG)

PTRT:

HU722_0006140(algG)

PXA:	KSS93_07765(algG)

PSAM:

HU731_020980(algG)

PTZ:	HU718_005885(algG)

PQI:	KH389_10430 KH389_22270(algG)

PSHH:

HU773_022860(algG)

PXN:	HU772_004395(algG) HU772_009380

PHV:	HU739_012735(algG)

PPRG:

HU725_004060(algG) HU725_014450

PANH:

HU763_020355(algG)

PASG:

KSS96_05670(algG)

PFAK:

KSS94_04590(algG)

PMUY:

KSS95_07450(algG) KSS95_11710

PMAM:

KSS90_04810(algG) KSS90_09855

PAZE:

KSS91_23870(algG)

PALV:

KSS97_06270(algG)

PORY:

EJA05_04775

PWZ:	J7655_04665(algG)

PTK:	EXN22_22980

PTW:	TUM18999_50170(algG)

PZA:	HU749_024165(algG)

PIE:	HU724_005645(algG)

PWY:	HU734_005455(algG)

PGF:	J0G10_06050(algG)

PCAV:

D3880_05520 D3880_05585

PJU:	L1P09_20875(algG)

PHF:	NLY38_05215(algG) NLY38_16770

PIZ:	LAB08_R50460(algG)

PPEG:

KUA23_05125(algG)

PSII:

NF676_05320(algG)

PSIH:

LOY51_04680(algG)

PCAS:

LOY40_05205(algG)

PFIT:

KJY40_05910(algG)

PASI:

LG197_00340(algG)

PKM:	PZ739_23475(algG)

PNB:	NK667_22360(algG)

PSOA:

PSm6_09970(algG)

PTAI:

ICN73_10925(algG)

PKK:	QQ992_23620(algG)

PKJ:	Q1W70_05495(algG) Q1W70_21890(algG)

PHYG:

JTY93_21640(algG)

PHOM:

KJF94_01525(algG) KJF94_13620(algG)

PCHE:

QYM18_12780(algG) QYM18_23060

PCUC:

PSH97_04615(algG)

PTRL:

OU419_22570(algG)

PPAE:

LDL65_16225(algG)

PPAO:

K3169_23675(algG)

PRHZ:

CRX69_09195

PSJY:

AA098_05000

PSKU:

KUIN1_10990

AVN:	Avin_10920(algG)

AVL:	AvCA_10920(algG)

AVD:	AvCA6_10920(algG)

ACX:	Achr_31230(algG)

PDEN:

F1C79_17975

PPHA:

BVH74_05265

MSR:	AU15_13695

HMI:	soil367_08840

ALKM:

NKI27_00770

CEG:	D0C16_10980

GIL:	NHM04_15525

ABO:	ABO_0394(algG)

ALN:	AS19_04070

GSN:	YC6258_00968

OME:	OLMES_4482

TAU:	Tola_1411 Tola_1417

REV:	HUE57_13025

PLG:	NCTC10937_04288(algG)

ARUI:

G6M88_17190

RHL:	LPU83_pLPU83d1240

MAAD:

AZF01_02695

MMED:

Mame_02308(algG)

MLUT:

JET14_03745

MALG:

MALG_02780

YPAC:

CEW88_18620

YAN:	AYJ57_24975

TOM:	BWR18_20755

THAA:

CFI11_06510

LSAL:

KBK07_17235

SECH:

B18_00690

NIS:

NIS_0188

CMED:

FE773_08540

CPAF:

C6V80_01905

PARH:

I5S86_05455(algG)

SPIN:

KV203_05825

FRE:	Franean1_6675

FRI:	FraEuI1c_0157 FraEuI1c_0161

SEN:

SACE_3182

SACG:

FDZ84_26120

PND:	Pla175_18190(algG)

CAO:	Celal_3935

CBAL:

M667_16165

CBAT:

M666_16150

TAM:	Theam_0793

NVN:	NVIE_010460

NEV:

NTE_02401

TAA:	NMY3_01609(algG)

NFN:	NFRAN_2449(algG)

» show all

Reference

1 [PMID:8144447]

Authors

Franklin MJ, Chitnis CE, Gacesa P, Sonesson A, White DC, Ohman DE

Title

Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase.

Journal

J Bacteriol 176:1821-30 (1994)
DOI:10.1128/JB.176.7.1821-1830.1994

Sequence

[pae:PA3545]

Reference

2 [PMID:11707327]

Authors

Morea A, Mathee K, Franklin MJ, Giacomini A, O'Regan M, Ohman DE

Title

Characterization of algG encoding C5-epimerase in the alginate biosynthetic gene cluster of Pseudomonas fluorescens.

Journal

Gene 278:107-14 (2001)
DOI:10.1016/S0378-1119(01)00685-0

Reference

3 [PMID:14526115]

Authors

Nyvall P, Corre E, Boisset C, Barbeyron T, Rousvoal S, Scornet D, Kloareg B, Boyen C

Title

Characterization of mannuronan C-5-epimerase genes from the brown alga Laminaria digitata.

Journal

Plant Physiol 133:726-35 (2003)
DOI:10.1104/pp.103.025981

Reference

4 [PMID:12581364]

Authors

Jain S, Franklin MJ, Ertesvag H, Valla S, Ohman DE

Title

The dual roles of AlgG in C-5-epimerization and secretion of alginate polymers in Pseudomonas aeruginosa.

Journal

Mol Microbiol 47:1123-33 (2003)
DOI:10.1046/j.1365-2958.2003.03361.x

Reference

5 [PMID:15968068]

Authors

Douthit SA, Dlakic M, Ohman DE, Franklin MJ

Title

Epimerase active domain of Pseudomonas aeruginosa AlgG, a protein that contains a right-handed beta-helix.

Journal

J Bacteriol 187:4573-83 (2005)
DOI:10.1128/JB.187.13.4573-4583.2005

Reference

6 [PMID:24398681]

Authors

Wolfram F, Kitova EN, Robinson H, Walvoort MT, Codee JD, Klassen JS, Howell PL

Title

Catalytic mechanism and mode of action of the periplasmic alginate epimerase AlgG.

Journal

J Biol Chem 289:6006-19 (2014)
DOI:10.1074/jbc.M113.533158

Sequence

[pst:PSPTO_1238]

Other DBs

ExplorEnz - The Enzyme Database:

5.1.3.37

IUBMB Enzyme Nomenclature:

5.1.3.37

ExPASy - ENZYME nomenclature database:

5.1.3.37

BRENDA, the Enzyme Database:

5.1.3.37

DBGET integrated database retrieval system