KEGG   ENZYME: 5.4.3.3
Entry
EC 5.4.3.3                  Enzyme                                 
Name
lysine 5,6-aminomutase;
beta-lysine 5,6-aminomutase;
beta-lysine mutase;
L-beta-lysine 5,6-aminomutase;
D-lysine 5,6-aminomutase;
D-alpha-lysine mutase;
adenosylcobalamin-dependent D-lysine 5,6-aminomutase
Class
Isomerases;
Intramolecular transferases;
Transferring amino groups
Sysname
(3S)-3,6-diaminohexanoate 5,6-aminomutase
Reaction(IUBMB)
(1) (3S)-3,6-diaminohexanoate = (3S,5S)-3,5-diaminohexanoate [RN:R03275];
(2) D-lysine = (2R,5S)-2,5-diaminohexanoate [RN:R02852]
Reaction(KEGG)
R02852 R03275
Substrate
(3S)-3,6-diaminohexanoate [CPD:C01142];
D-lysine [CPD:C00739]
Product
(3S,5S)-3,5-diaminohexanoate [CPD:C01186];
(2R,5S)-2,5-diaminohexanoate [CPD:C05161]
Comment
This enzyme is a member of the 'AdoMet radical' (radical SAM) family. It requires pyridoxal 5'-phosphate and adenosylcobalamin for activity. A 5'-deoxyadenosyl radical is generated during the reaction cycle by reductive cleavage of adenosylcobalamin, which is regenerated at the end of the reaction.
History
EC 5.4.3.3 created 1972 (EC 5.4.3.4 created 1972, incorporated 2017), modified 2017
Pathway
ec00310  Lysine degradation
ec00470  D-Amino acid metabolism
ec01100  Metabolic pathways
Orthology
K01844  beta-lysine 5,6-aminomutase alpha subunit
K18011  beta-lysine 5,6-aminomutase beta subunit
Genes
RFRRfer_3887 Rfer_3888
NOKFAY22_04155 FAY22_04160
ARESIWH25_08670 IWH25_08675
APETToN1_41370 ToN1_41380
AZAAZKH_1576 AZKH_1577
AZIAzCIB_1068 AzCIB_1069
DEKDSLASN_49400 DSLASN_49410
ADEAdeh_1483 Adeh_1485
ACPA2cp1_2470 A2cp1_2472
AFWAnae109_2347 Anae109_2349
ANKAnaeK_2382 AnaeK_2384
MXAMXAN_4386(kamE) MXAN_4388(kamD)
MSDMYSTI_04875 MYSTI_04877
MYMA176_002515 A176_002517
MFBMFUL124B02_26680 MFUL124B02_26690
CCXCOCOR_03525(kamD) COCOR_03527(kamE)
MFULILAB_30020 LILAB_30030
MMASMYMAC_004300 MYMAC_004302
SURSTAUR_4746(kamE) STAUR_4748(kamD)
AGEAA314_04546 AA314_04548
AVMJQX13_36710 JQX13_36720
MBDMEBOL_007833 MEBOL_007835
VINAKJ08_2038 AKJ08_2039
SCLsce2889(kamD) sce2891(kamE)
SCUSCE1572_17625 SCE1572_17630
CCROCMC5_058650 CMC5_058660
PAUUE8A73_024820 E8A73_024825
LLUAKJ09_09143 AKJ09_09144
MRMA7982_03845 A7982_03847
BSEDDN745_06625 DN745_14905
PCAYFRD00_11200 FRD00_21455
BBAEFRD01_07905 FRD01_18255
AFXJZ786_00820 JZ786_00825
ACURJZ785_19365 JZ785_19370
TUMCBW65_05610 CBW65_05615
TABCIG75_01555 CIG75_01560
LFBC1X05_16395 C1X05_16400
TVUAB849_005135 AB849_005140
KPULGXN76_15745 GXN76_15750
KEBGXN75_17080 GXN75_17085
PABSJIR001_31210 JIR001_31220
CLSCXIVA_06620 CXIVA_06630
CPATCLPA_c31440(kamE) CLPA_c31450(kamD)
CPAECPAST_c31440(kamE) CPAST_c31450(kamD)
CEUA7L45_04310 A7L45_04315
CARGRSJ17_19885 RSJ17_19890
CCAAKQH81_17185 KQH81_17190
CRWCROST_000220(kamD) CROST_000230(kamE)
CFBCLFE_002770(kamE) CLFE_002780(kamD)
AMTAmet_4548 Amet_4549
AOEClos_0421 Clos_0422
GFEGferi_11510 Gferi_11515
CRSFQB35_13830
CPRFK7H06_00045 K7H06_00050
FPLAA4U99_02910 A4U99_02915
VFAMM35RIKEN_07320 MM35RIKEN_07330
VCOPMM50RIKEN_12190 MM50RIKEN_12200
LUAD4A81_03630 D4A81_03635
CSTCLOST_1378(kamE) CLOST_1379(kamD)
TEBT8CH_2398(kamE) T8CH_2399
STHSTH37 STH38
DAIDesaci_1866 Desaci_1867
FWADCMF_21040 DCMF_21045
AACXDEACI_2074
VGUHYG85_18550 HYG85_18555
VPYHZI73_17975 HZI73_17980
ACAEHYG86_10315 HYG86_10320
IBUIB211_00981 IB211_00982
AMIJEQM06_11635 EQM06_11640
AMICAmi3637_09155 Ami3637_09160
ABUTAmi103574_14985 Ami103574_14990
ETMCE91St48_07420 CE91St48_07430
EUUADJ67_02650 ADJ67_02655
TTETTE0726 TTE0727
TWIThewi_0855 Thewi_0856
NTHNther_1905 Nther_1906
HPKHprae_1560 Hprae_1561
HALSD7D81_18055 D7D81_18060
IFNGM661_14620 GM661_14625
AFTBBF96_12065 BBF96_12070
KMEH0A61_00949 H0A61_00950(kamD)
SPOAEQM13_02805 EQM13_02810
KPARJL105_00845 JL105_00850
CAZOG3A45_10725 G3A45_10730
SEDZJYG23_11320 JYG23_11325
PUFUFO1_1640 UFO1_1641
PFTJBW_02724 JBW_02725
MANRMPAN_015970 MPAN_015980
MBJKQ51_00865(kamD) KQ51_00866(kamE)
TBMBK010_00755 BK010_00760
TBZBK011_00655 BK011_00660
TBKHF295_06590 HF295_06595
SCXAS200_02795
ICAIntca_1791 Intca_1792
ARSADJ73_12505 ADJ73_12510
TEHGKE56_04785 GKE56_04790
PHWG7075_06380 G7075_06385
PEIH9L10_06015
SERWFY030_07855 FY030_07860
ORNDV701_02935 DV701_02940
NCANoca_2663 Noca_2664
NOYEXE57_02160 EXE57_02165
NOIFCL41_08530 FCL41_08535
NOOFE634_10520 FE634_10525
NDPE2C04_09130 E2C04_09135
NSNEXE58_17595 EXE58_17600
NANOG5V58_11085 G5V58_11090
NMESH9L09_20180 H9L09_20185
NPIG7071_14780
NAQUENKNEFLB_02384(kamE) ENKNEFLB_02385(kamD)
NROK8W59_08665 K8W59_08670
NOQLN652_02045 LN652_02050
NCQK6T13_09125 K6T13_09130
KFLKfla_3601 Kfla_3602
KQIF1D05_03990 F1D05_03995
TCUTcur_2347
AGRAAGRA3207_000441 AGRA3207_000442
MMARMODMU_2631 MODMU_2632
SESPBN6_43170 BN6_43180
SSYIEKG83_24030 EKG83_24035
KALKALB_4418 KALB_4419
KPHYAOZ06_27985 AOZ06_27990
LEDBBK82_41115 BBK82_41120
ALOCRK56464 CRK56465
STPStrop_2265 Strop_2266
SAQSare_2384 Sare_2385
MAUMicau_3122 Micau_3123
MILML5_5272 ML5_5273
MICBMicB006_2176 MicB006_2177
MTUACSH63_09500 CSH63_09505
MICHFJK98_17890 FJK98_17895
MTEMGCE86_10830 GCE86_10835
MCABHXZ27_19850 HXZ27_19855
MSAGGCM10017556_54740 GCM10017556_54750
VMAVAB18032_20380 VAB18032_20385
ASEACPL_2802(kamE) ACPL_2803
AMSAMIS_47590 AMIS_47600
ACTNL083_3927 L083_3928(kamE)
AFSAFR_21805 AFR_21810
ACTSACWT_2743 ACWT_2744
AIHAiant_75460 Aiant_75470
PLKCIK06_14140 CIK06_14145
PLABC6361_03355
PLATC6W10_18270
PFLAPflav_024670 Pflav_024700
PSUUPsuf_079150 Psuf_079160
VERHUT12_17880 HUT12_17885
ATLAthai_29170 Athai_29180
ASERAsera_45360 Asera_45370
PRYPrubr_60570
CATICS0771_51100 CS0771_51110
DVCDvina_27770 Dvina_27775
NHYJQS43_13150 JQS43_13155
CCAZCOUCH_20865 COUCH_20870
SNASnas_3949 Snas_3950
NAVJQS30_07340 JQS30_07345
GLYK3N28_19455 K3N28_19460
FNUFN1862 FN1863
FNCHMPREF0946_01610 HMPREF0946_01611
FNTHMPREF0405_01367 HMPREF0405_01368
FUSHMPREF0409_01390 HMPREF0409_01391
FNEFSDG_01978 FSDG_01979
FHWRN87_00375 RN87_00380
FPDCTM68_06750 CTM68_06755
FVAFV113G1_13810 FV113G1_13820
FULC4N20_15435 C4N20_15440
FGOC4N16_05160 C4N16_05165
FNFBSQ88_03875 BSQ88_03880
FPEIC4N17_12385 C4N17_12390
FCII6I83_03030 I6I83_03035
PGIPG_1073(kamD) PG_1074(kamE)
PGNPGN_1169 PGN_1170
PGTPGTDC60_1134(kamE) PGTDC60_1135(kamD)
PAHPoras_0579 Poras_0580
PCRENCTC12858_01320 NCTC12858_01321
PCAGNCTC12856_01008 NCTC12856_01009
PENDLA319_06765 LA319_06770
PSOECE91St14_15540 CE91St14_15550
PETPEIBARAKI_4145 PEIBARAKI_4146
OSPOdosp_3176 Odosp_3177
BUYD8S85_10245 D8S85_10250
BVIRI6J59_05580 I6J59_05585
BACCBRDCF_p1067 BRDCF_p1068
MBASALGA_2260 ALGA_2261
CACICLOAM1503(kamE) CLOAM1504(kamD)
TLETlet_1712 Tlet_1713
PHYAJ81_06585 AJ81_06590
TMETmel_0196 Tmel_0197
TAFTHA_428 THA_429
THPBG95_02115 BG95_02120
THERY592_02120 Y592_02125
TFEJYK00_06010 JYK00_06015
FNOFnod_0202 Fnod_0203
FPEFerpe_0545 Ferpe_0546
FIANA23_03040 NA23_03045
OCYOSSY52_05610 OSSY52_05620
PMOPmob_1084 Pmob_1085
MPZMarpi_0482 Marpi_0483
MARNLN42_02785 LN42_02790
KOLKole_0958 Kole_0959
KPFIX53_04790 IX53_04795
MPGTheba_2106 Theba_2107
MINFMESINF_1640(kamD) MESINF_1641
ASACATHSA_1213 ATHSA_1214
PSYTDSAG12_00552(mtbC_2) DSAG12_00553
 » show all
Reference
1  [PMID:4229021]
  Authors
Stadtman TC, Tsai L.
  Title
A cobamide coenzyme dependent migration of the epsilon-amino group of D-lysine.
  Journal
Biochem Biophys Res Commun 28:920-6 (1967)
DOI:10.1016/0006-291X(67)90067-8
Reference
2  [PMID:5649516]
  Authors
Stadtman TC, Renz P.
  Title
Anaerobic degradation of lysine. V. Some properties of the cobamide coenzyme-dependent beta-lysine mutase of Clostridium sticklandii.
  Journal
Arch Biochem Biophys 125:226-39 (1968)
DOI:10.1016/0003-9861(68)90657-7
Reference
3  [PMID:5480154]
  Authors
Morley CG, Stadtman TC.
  Title
Studies on the fermentation of D-alpha-lysine. Purification and properties of an adenosine triphosphate regulated B 12-coenzyme-dependent D-alpha-lysine mutase complex from Clostridium sticklandii.
  Journal
Biochemistry 9:4890-900 (1970)
DOI:10.1021/bi00827a010
Reference
4
  Authors
Retey, J., Kunz, F., Arigoni, D. and Stadtman, T.C.
  Title
Zur Kenntnis der beta-Lysin-Mutase-Reaktion: mechanismus und sterischer Verlauf.
  Journal
Helv Chim Acta 61:2989-2998 (1978)
Reference
5  [PMID:10617592]
  Authors
Chang CH, Frey PA
  Title
Cloning, sequencing, heterologous expression, purification, and characterization  of adenosylcobalamin-dependent D-lysine 5, 6-aminomutase from Clostridium sticklandii.
  Journal
J Biol Chem 275:106-14 (2000)
DOI:10.1074/jbc.275.1.106
  Sequence
Reference
6  [PMID:12093296]
  Authors
Tang KH, Harms A, Frey PA
  Title
Identification of a novel pyridoxal 5'-phosphate binding site in adenosylcobalamin-dependent lysine 5,6-aminomutase from Porphyromonas gingivalis.
  Journal
Biochemistry 41:8767-76 (2002)
DOI:10.1021/bi020255k
Reference
7  [PMID:19634897]
  Authors
Tang KH, Mansoorabadi SO, Reed GH, Frey PA
  Title
Radical triplets and suicide inhibition in reactions of 4-thia-D- and 4-thia-L-lysine with lysine 5,6-aminomutase.
  Journal
Biochemistry 48:8151-60 (2009)
DOI:10.1021/bi900828f
Reference
8  [PMID:15514022]
  Authors
Berkovitch F, Behshad E, Tang KH, Enns EA, Frey PA, Drennan CL
  Title
A locking mechanism preventing radical damage in the absence of substrate, as revealed by the x-ray structure of lysine 5,6-aminomutase.
  Journal
Proc Natl Acad Sci U S A 101:15870-5 (2004)
DOI:10.1073/pnas.0407074101
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.4.3.3
IUBMB Enzyme Nomenclature: 5.4.3.3
ExPASy - ENZYME nomenclature database: 5.4.3.3
BRENDA, the Enzyme Database: 5.4.3.3
CAS: 9075-69-8

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