KEGG   ENZYME: 5.4.99.64
Entry
EC 5.4.99.64                Enzyme                                 

Name
2-hydroxyisobutanoyl-CoA mutase;
hcmAB (gene names)
Class
Isomerases;
Intramolecular transferases;
Transferring other groups
Sysname
2-hydroxy-2-methylpropanoyl-CoA mutase
Reaction(IUBMB)
2-hydroxy-2-methylpropanoyl-CoA = (S)-3-hydroxybutanoyl-CoA [RN:R11334]
Reaction(KEGG)
R11334
Substrate
2-hydroxy-2-methylpropanoyl-CoA [CPD:C21287]
Product
(S)-3-hydroxybutanoyl-CoA [CPD:C01144]
Comment
The enzyme, characterized from the bacterium Aquincola tertiaricarbonis, uses radical chemistry to rearrange the positions of both a methyl group and a hydroxyl group. It consists of two subunits, the smaller one containing a cobalamin cofactor. It plays a central role in the degradation of assorted substrates containing a tert-butyl moiety.
History
EC 5.4.99.64 created 2016 as EC 5.3.3.20, transferred 2017 to EC 5.4.99.64
Orthology
K20906  2-hydroxyisobutanoyl-CoA mutase large subunit
K20907  2-hydroxyisobutanoyl-CoA mutase small subunit
Genes
MSR: AU15_14560 AU15_14575
MPT: Mpe_B0538 Mpe_B0541
XAU: Xaut_5018 Xaut_5021
SNO: Snov_2770 Snov_2773
RSH: Rsph17029_3654 Rsph17029_3657
RSK: RSKD131_3113 RSKD131_3116
Reference
1  [PMID:22433853]
  Authors
Yaneva N, Schuster J, Schafer F, Lede V, Przybylski D, Paproth T, Harms H, Muller RH, Rohwerder T
  Title
Bacterial acyl-CoA mutase specifically catalyzes coenzyme B12-dependent isomerization of 2-hydroxyisobutyryl-CoA and (S)-3-hydroxybutyryl-CoA.
  Journal
J Biol Chem 287:15502-11 (2012)
DOI:10.1074/jbc.M111.314690
  Sequence
Reference
2  [PMID:25720495]
  Authors
Kurteva-Yaneva N, Zahn M, Weichler MT, Starke R, Harms H, Muller RH, Strater N, Rohwerder T
  Title
Structural basis of the stereospecificity of bacterial B12-dependent 2-hydroxyisobutyryl-CoA mutase.
  Journal
J Biol Chem 290:9727-37 (2015)
DOI:10.1074/jbc.M115.645689
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.4.99.64
IUBMB Enzyme Nomenclature: 5.4.99.64
ExPASy - ENZYME nomenclature database: 5.4.99.64
BRENDA, the Enzyme Database: 5.4.99.64

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