KEGG   ENZYME: 5.5.1.27
Entry
EC 5.5.1.27                 Enzyme                                 

Name
D-galactarolactone cycloisomerase;
GCI
Class
Isomerases;
Intramolecular lyases;
Intramolecular lyases (only sub-subclass identified to date)
Sysname
D-galactaro-1,4-lactone lyase (ring-opening)
Reaction(IUBMB)
(1) D-galactaro-1,4-lactone = 5-dehydro-4-deoxy-D-glucarate [RN:R10847];
(2) D-glucaro-1,4-lactone = 5-dehydro-4-deoxy-D-glucarate [RN:R11083]
Reaction(KEGG)
R10847 R11083
Substrate
D-galactaro-1,4-lactone [CPD:C20896];
D-glucaro-1,4-lactone [CPD:C21095]
Product
5-dehydro-4-deoxy-D-glucarate [CPD:C00679]
Comment
The enzyme, characterized from the bacterium Agrobacterium fabrum strain C58, is involved in degradation of D-galacturonate and D-glucuronate. Activity with D-galactaro-1,4-lactone is 4-fold higher than with D-glucaro-1,4-lactone.
History
EC 5.5.1.27 created 2015
Pathway
ec00053  Ascorbate and aldarate metabolism
ec01100  Metabolic pathways
Orthology
K18983  D-galactarolactone cycloisomerase
Genes
EAL: EAKF1_ch1998c
CAMA: F384_17470
GQU: AWC35_04195
IZH: FEM41_12900
SOD: Sant_1026 Sant_2261
PAO: Pat9b_4431
KLN: LH22_06000
PANS: FCN45_23420
PSI: S70_13550
PSX: DR96_551
PRG: RB151_022700(rhmD)
VSC: VSVS12_03474(lra3)
SPL: Spea_3858
SHL: Shal_0409
PAT: Patl_0897
FBL: Fbal_0608
GAI: IMCC3135_03235(gci_1) IMCC3135_28780(gci_2)
CSA: Csal_2476
HEL: HELO_3407(gci)
HAM: HALO3643
ADI: B5T_03504
SALN: SALB1_0470
CGD: CR3_3860
BPAR: BN117_4287
BPA: BPP1390
BPT: Bpet2903
ODI: ODI_R1804
POL: Bpro_1862
DAT: HRM2_22830(mdlA)
MES: Meso_1946
ATU: Atu3139
ATF: Ach5_29940(gci)
BRS: S23_45390
BRAD: BF49_1895
RPB: RPB_4061
VGO: GJW-30_1_00576(rhmD)
XAU: Xaut_3370
PHL: KKY_3040
SIL: SPO2414
RUT: FIU92_10665(yitF)
RDE: RD1_0679
RHC: RGUI_3938
SPSE: SULPSESMR1_04919(gci)
SSAN: NX02_16030
SHUM: STHU_30510(gci_2) STHU_32630
SVC: STVA_18940 STVA_20350(gci_2)
BSS: BSUW23_05560(yitF)
BST: GYO_1403(yitF)
BLI: BL01977(yitF)
BLD: BLi02157(yitF)
BLH: BaLi_c22320(yitF)
BGY: BGLY_2291
BBE: BBR47_38150(yitF)
BLR: BRLA_c034680(yitF)
MANA: MAMMFC1_04136(yitF)
SBH: SBI_00752
SMAL: SMALA_7628
MOY: CVS54_01196(gci)
BCV: Bcav_0644
KFL: Kfla_2398
PSIM: KR76_00300
SRO: Sros_5741
SEN: SACE_2582
PAUT: Pdca_64030
ACTI: UA75_20035
ACAD: UA74_19545
TTR: Tter_2214
PLS: VT03_23450(yitF)
PLH: VT85_12270(yitF)
FMR: Fuma_05789(yitF)
GMR: GmarT_18570(yitF)
GES: VT84_20960(rhmD)
SACI: Sinac_0917
AGV: OJF2_00180(yitF)
SUS: Acid_7728
ABAC: LuPra_02649(dgoD_3)
MBAS: ALGA_2221
MARM: YQ22_09895
CBAL: M667_19555
CBAT: M666_19475
ZGA: ZOBELLIA_3156(dgoD)
KAN: IMCC3317_46240(gci)
HTU: Htur_4435
VDI: Vdis_0390
 » show all
Reference
1  [PMID:22493433]
  Authors
Andberg M, Maaheimo H, Boer H, Penttila M, Koivula A, Richard P
  Title
Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate.
  Journal
J Biol Chem 287:17662-71 (2012)
DOI:10.1074/jbc.M111.335240
  Sequence
[atu:Atu3139]
Reference
2  [PMID:24450804]
  Authors
Bouvier JT, Groninger-Poe FP, Vetting M, Almo SC, Gerlt JA
  Title
Galactaro delta-lactone isomerase: lactone isomerization by a member of the amidohydrolase superfamily.
  Journal
Biochemistry 53:614-6 (2014)
DOI:10.1021/bi5000492
  Sequence
[atu:Atu3139]
Other DBs
ExplorEnz - The Enzyme Database: 5.5.1.27
IUBMB Enzyme Nomenclature: 5.5.1.27
ExPASy - ENZYME nomenclature database: 5.5.1.27
BRENDA, the Enzyme Database: 5.5.1.27

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