KEGG ENZYME: 5.5.1.27

ENZYME: 5.5.1.27

Entry

EC 5.5.1.27                 Enzyme

Name

D-galactarolactone cycloisomerase;
GCI

Class

Isomerases;
Intramolecular lyases;
Intramolecular lyases (only sub-subclass identified to date)

Sysname

D-galactaro-1,4-lactone lyase (ring-opening)

Reaction(IUBMB)

(1) D-galactaro-1,4-lactone = 5-dehydro-4-deoxy-D-glucarate [RN:R10847];
(2) D-glucaro-1,4-lactone = 5-dehydro-4-deoxy-D-glucarate [RN:R11083]

Reaction(KEGG)

R10847 R11083

Substrate

D-galactaro-1,4-lactone [CPD:C20896];
D-glucaro-1,4-lactone [CPD:C21095]

Product

5-dehydro-4-deoxy-D-glucarate [CPD:C00679]

Comment

The enzyme, characterized from the bacterium Agrobacterium fabrum strain C58, is involved in degradation of D-galacturonate and D-glucuronate. Activity with D-galactaro-1,4-lactone is 4-fold higher than with D-glucaro-1,4-lactone.

History

EC 5.5.1.27 created 2015

Pathway

ec00053

Ascorbate and aldarate metabolism

ec01100

Metabolic pathways

Orthology

K18983

D-galactarolactone cycloisomerase

Genes

CAMA:

F384_17470

CTEL:

GBC03_28085

IZH:	FEM41_12900

SUPE:

P0H77_06750

TOE:	QMG90_04975

GQU:	AWC35_04195

SOD:	Sant_1026 Sant_2261

SLIG:

GTU79_12705

PAO:	Pat9b_4431

KLN:	LH22_06000

PANS:

FCN45_23420

PSI:

S70_13550

PSX:

DR96_551

PSTA:

BGK56_16010

PRG:	RB151_022700(rhmD)

PRQ:	CYG50_05295

PVC:	G3341_16965

PTHA:

OI982_10105

VNI:	VIBNI_B0980

VSC:	VSVS12_03474(lra3)

VAF:	D1115_10435

VAS:	GT360_19110

PGB:	H744_1c0304

SPL:

Spea_3858

SHL:

Shal_0409

SJA:	SJ2017_1939

COLW:

A3Q33_18040

THT:	E2K93_07385

PBW:	D172_007745

PCAR:

PC2016_3905

PMES:

FX988_01827

PAT:

Patl_0897

CATE:

C2869_00665

FBL:

Fbal_0608

MTHD:

A3224_10175

MAGA:

Mag101_05735

SROS:

BBH56_04565

GAI:	IMCC3135_03235(gci_1) IMCC3135_28780(gci_2)

CSA:

Csal_2476

HEL:	HELO_3407(gci)

HAK:	KO116_03370

HAM:

HALO3643

HOL:	HORIV_64370

HTT:	HZS52_07800

HCAM:

I4484_00185

HSX:	HNO51_00185

HSV:	HNO53_00185

HTX:	EKK97_00190

HHAO:

QWG60_12690

KUY:	FY550_00795

ADI:

B5T_03504

MME:	Marme_0065

MPC:	Mar181_2906

MARS:

A8C75_18900

LLP:	GH975_04805 GH975_07765

SALN:

SALB1_0470

CGD:

CR3_3860

PGP:	CUJ91_31590

PTRO:

G5S35_31625

PACS:

FAZ98_10555 FAZ98_30730

BPAR:

BN117_4287

BPA:

BPP1390

BBH:	BN112_0281 BN112_3736

BBR:	BB2459 BB4687

BBM:	BN115_2658

BBX:	BBS798_2295 BBS798_4463

BPT:

Bpet2903

BBRO:

BAU06_00140 BAU06_14035 BAU06_14065

BFZ:	BAU07_12100 BAU07_12145

BOH:	AKI39_13245 AKI39_13285 AKI39_13430

BGV:	CAL12_14790

PUS:	CKA81_13890

AKA:	TKWG_00290

ODI:

ODI_R1804

PARK:

LSG25_04810

VAP:	Vapar_5519

VPD:	VAPA_2c05200

DRG:	H9K76_08570

XYK:	GT347_26065

ATW:	C0099_01355

MESW:

A9K65_013755

MESP:

C1M53_17420

MES:

Meso_1946

RPOD:

E0E05_16250

NKI:	KW403_06520

RHI:	NGR_b14290

SFH:	SFHH103_05618

EMX:	FKV68_21235

EAD:	OV14_a1863 OV14_b0357

EAH:	FA04_25485 FA04_30525

ECAA:

J3R84_28135 J3R84_35615

ENP:	JVX98_06870

ATU:

Atu3139

ATA:	AWN88_10710

AGR:	AGROH133_10632

ATF:	Ach5_29940(gci)

AGC:	BSY240_2211

AGT:	EYD00_14440

ALF:	CFBP5473_09270

ARUI:

G6M88_13790

AVQ:	HRR99_06015

ASAL:

CFBP5507_23260

RIR:	BN877_II0123

RPUS:

CFBP5875_14590

ACUC:

KZ699_15130

ALEG:

CFBP4996_14970

RLB:	RLEG3_27710

RHV:	BA939_26325

RJG:	CCGE525_24245 CCGE525_30040

RHR:	CKA34_27150 CKA34_27745

ROY:	G3A56_17325

RII:	FFM53_017765

RROS:

D4A92_09815

RRHO:

PR018_24155

RTU:	PR017_24995

ARO:	B0909_22900

NGL:	RG1141_CH22490

NGG:	RG540_CH23330

NEO:	CYG48_08760

NEN:	NCHU2750_21870(gci) NCHU2750_42880 NCHU2750_47880 NCHU2750_53580

NPM:	QEO92_11840

SHZ:

shn_28795

SZO:	K8M09_21620

SOJ:	K6301_22790

PDES:

FE840_018515

BJU:	BJ6T_69710

BJP:	RN69_33820

BRC:	BCCGELA001_12525

BRAD:

BF49_1895

BIC:	LMTR13_17465

BOT:	CIT37_35350

BGQ:	X265_26885

BARH:

WN72_15330

BBAN:

J4G43_016105

BCAN:

BcanWSM471_13320

BBRA:

QA636_34465

BGK:	IC762_21520

BCOU:

IC761_25335

BXN:	I3J27_10320

RBK:	E0H22_13950

VGO:	GJW-30_1_00576(rhmD)

BOP:	AXW83_04155 AXW83_25440 AXW83_26310

BVV:	BHK69_06615 BHK69_14610

BOI:	BLM15_17215 BLM15_23225

BOF:	FQV39_20260

BVES:

QO058_13540

XAU:

Xaut_3370

XDI:	EZH22_23385

SNO:	Snov_4076 Snov_4090

STAR:

G3545_11175 G3545_16275 G3545_16295 G3545_27185

LNE:	FZC33_19190

APRA:

G3A50_04735

MMES:

MMSR116_04700

MOC:	BB934_24410

CHEL:

AL346_24405

CDQ:	BOQ54_18660

PHL:

KKY_3040

DEQ:	XM25_20980

DEA:	FPZ08_20120

YTI:	FNA67_02305

JIE:	OH818_20375

NOH:	G5V57_20170

PSTG:

E8M01_20230

ACUT:

MRB58_08345 MRB58_11990

SIL:

SPO2414

RUA:	D1823_06565

RUT:	FIU92_10665(yitF)

RCON:

K3740_04780

RDE:

RD1_0679

RLI:	RLO149_c038620

OAT:	OAN307_c19320

OAR:	OA238_c47540 OA238_c47610

OCT:	FTO60_03720

MALG:

MALG_00963 MALG_02603

YPAC:

CEW88_05335

YAN:	AYJ57_18565

TPRO:

Ga0080559_TMP359 Ga0080559_TMP419

SULZ:

C1J03_24785

SUAL:

KDD17_17655

SPSE:

SULPSESMR1_04919(gci)

AHT:	ANTHELSMS3_02587

RSH:	Rsph17029_4153

PSEW:

JHW44_16960

PAEZ:

PAE61_04995

PTP:	RCA23_c23230 RCA23_c23350

RHC:

RGUI_3938

LIT:	FPZ52_00260

PPRU:

FDP22_06925

PSHQ:

F3W81_11305

PALW:

PSAL_035960(gci)

HDH:	G5B40_00590 G5B40_03710 G5B40_08685

CMAG:

CBW24_05885

SSAN:

NX02_16030

SPLM:

BXU08_01130

SPHC:

CVN68_11675

SPHL:

LPB140_03495

EGN:	BMF35_a0049 BMF35_a0055 BMF35_a0333

PHZ:	CHX26_04735

RCV:	PFY06_07215

SRF:	LHU95_06515

RAP:

RHOA_4870

SHUM:

STHU_30510(gci_2) STHU_32630

SVC:	STVA_18940 STVA_20350(gci_2)

STEL:

STAQ_17870(gci) STAQ_29340

ABS:	AZOBR_p310126

ABQ:	ABAZ39_25585

AZT:	TSH58p_01545

AARE:

D3093_24830

NACI:

NUH88_05275

DFI:	AXF13_00735

BSS:	BSUW23_05560(yitF)

BST:	GYO_1403(yitF)

BIT:	BIS30_16195

BLI:	BL01977(yitF)

BLD:	BLi02157(yitF)

BLH:	BaLi_c22320(yitF)

BAE:	BATR1942_03120

BGY:

BGLY_2291

BBE:	BBR47_38150(yitF)

BLR:	BRLA_c034680(yitF)

BFM:	BP422_14390

BAGR:

BA6348_12065

BRW:	GOP56_09425

BCHS:

JNE38_19360

PNP:	IJ22_01170 IJ22_15690

PALO:

E6C60_0203

NTR:	B0W44_15605

KPUL:

GXN76_07985

PABS:

JIR001_24760(yitF)

DRU:	Desru_2534

TFR:	BR63_01905 BR63_13330

FWA:	DCMF_06330

VPY:	HZI73_07910

GEK:	kuro4_13840(gci)

LPIL:

LIP_0420 LIP_0509 LIP_1982

ERZ:	ER308_01805 ER308_15350

SVL:	Strvi_4313

SRC:	M271_03750

SMAL:

SMALA_7628

SBH:

SBI_00752

SXI:	SXIM_20370

SCHA:

CP983_06225

SGX:	H4W23_01580

SHAR:

HUT13_08940

MOY:	CVS54_01196(gci)

AGY:	ATC03_14870

SALA:

ESZ53_09205

HEA:	HL652_18515

ARZ:	AUT26_19340

ARN:	CGK93_10790

ARX:	ARZXY2_4041

PUE:	FV140_21085

GAR:	AOZ07_00220

SATK:

SA2016_0899

BCV:

Bcav_0644

BRX:	BH708_16440 BH708_16790

BGG:	CFK41_13980

CET:	B8281_01575

MIK:	FOE78_04480

MICG:

GJV80_15955

PROP:

QQ658_00260

BROO:

brsh051_13670

PSIM:

KR76_00300

KFL:

Kfla_2398

NOA:	BKM31_21020

NAK:	EH165_12545

SEN:

SACE_2582

PAUT:

Pdca_64030

ACTI:

UA75_20035

ACAD:

UA74_19545

PMAD:

BAY61_16475

MTEM:

GCE86_03285

PSUU:

Psuf_090430

ATL:	Athai_20320 Athai_45540

PVS:	A1sIA79_01995 A1sIA79_06480

TTR:

Tter_2214

PPHT:

GA004_05350

VBS:	EGM51_06115

AAGG:

ETAA8_24950(rhmD)

AMOB:

HG15A2_17800(yitF)

PLS:	VT03_23450(yitF)

PLH:	VT85_12270(yitF)

FMR:	Fuma_05789(yitF)

GMR:	GmarT_18570(yitF)

MRI:	Mal4_04010

SDYN:

Mal52_00340(yitF)

GES:	VT84_20960(rhmD)

GMS:	SOIL9_12620

TIM:	GMBLW1_25140

ULI:	ETAA1_22390(rhmD) ETAA1_55000(dgoD_4)

SACI:

Sinac_0917

AGV:	OJF2_00180(yitF)

PFER:

IRI77_24375

ABAC:

LuPra_02649(dgoD_3)

MCOS:

GM418_07785

MBAS:

ALGA_2221

CHIT:

FW415_11695

COY:	HF329_06185

HHY:	Halhy_2207

PGS:	CPT03_08685

SHT:	KO02_11610 KO02_15740

SPHN:

BV902_21785

STHA:

NCTC11429_00215

MMAB:

HQ865_19795

SBX:	CA265_12255

CMR:	Cycma_3933

EOL:	Emtol_1245

MAQA:

LAG90_11920

FLM:	MY04_5029 MY04_5048

FLL:	EI427_16820 EI427_21965

FYA:	KMW28_27615 KMW28_27710

FKA:	KM029_22790 KM029_26030

TPEL:

P0M28_08485

FFA:	FFWV33_17870

MARM:

YQ22_09895

MART:

BTR34_09960

MARB:

CJ263_06230

MARE:

EJ994_11795

MLIO:

NYZ99_01230

CAO:	Celal_0417

CLY:	Celly_1807

CLH:	IX49_08920

CBAL:

M667_19555

CBAT:

M666_19475

COMN:

PBN93_08945

ZGA:	ZOBELLIA_3156(dgoD)

ZLA:	Q5W13_15625

PUN:	NQP51_16915

TJE:	TJEJU_2926

WFU:	AXE80_10120

FOH:	FORMA_02450

TAJ:	C1A40_08335

KAN:	IMCC3317_46240(gci)

GAA:	HX109_10180

FBE:	FF125_03875

HALX:

M0R89_21380

HALJ:

G9465_20630

HPEL:

HZS54_10535

HGI:	ABY42_15285

HAQ:	DU484_06805

HAJ:	DU500_00255 DU500_07075

HAER:

DU502_04740

HRA:	EI982_05865 EI982_09005

HALQ:

Hrr1229_004955

SRUB:

C2R22_00735

HALG:

HUG10_06970 HUG10_07125 HUG10_11805 HUG10_15365

HALU:

HUG12_05215 HUG12_08520 HUG12_17675

HMP:	K6T50_03860

HRE:	K6T36_17035

HTU:

Htur_4435

HSAL:

JMJ58_23585

HXA:	Halxa_0385

NOU:	Natoc_1246

HLR:	HALLA_00610 HALLA_04440

VDI:

Vdis_0390

» show all

Reference

1 [PMID:22493433]

Authors

Andberg M, Maaheimo H, Boer H, Penttila M, Koivula A, Richard P

Title

Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate.

Journal

J Biol Chem 287:17662-71 (2012)
DOI:10.1074/jbc.M111.335240

Sequence

[atu:Atu3139]

Reference

2 [PMID:24450804]

Authors

Bouvier JT, Groninger-Poe FP, Vetting M, Almo SC, Gerlt JA

Title

Galactaro delta-lactone isomerase: lactone isomerization by a member of the amidohydrolase superfamily.

Journal

Biochemistry 53:614-6 (2014)
DOI:10.1021/bi5000492