KEGG   ENZYME: 5.5.1.34
Entry
EC 5.5.1.34                 Enzyme                                 

Name
(+)-cis,trans-nepetalactol synthase;
NEPS1 (gene name);
NEPS2 (gene name)
Class
Isomerases;
Intramolecular lyases;
Intramolecular lyases (only sub-subclass identified to date)
Sysname
(S)-8-oxocitronellyl enol cyclase [(+)-cis,trans-nepetalactol-forming]
Reaction(IUBMB)
(S)-8-oxocitronellyl enol = (+)-cis,trans-nepetalactol [RN:R12520]
Reaction(KEGG)
R12520
Substrate
(S)-8-oxocitronellyl enol [CPD:C22225]
Product
(+)-cis,trans-nepetalactol [CPD:C09804]
Comment
The enzyme, characterized from the plant Nepeta mussinii, binds an NAD+ cofactor. The product is a precursor of (+)-cis,trans-nepetalactone, the primary ingredient responsible for the psychoactive effects catnip has on cats.
History
EC 5.5.1.34 created 2019
Pathway
ec00902  Monoterpenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K23862  (+)-cis,trans-nepetalactol synthase
K23990  nepetalactol dehydrogenase / (+)-cis,trans-nepetalactol synthase
Reference
1  [PMID:30531909]
  Authors
Lichman BR, Kamileen MO, Titchiner GR, Saalbach G, Stevenson CEM, Lawson DM, O'Connor SE
  Title
Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis.
  Journal
Nat Chem Biol 15:71-79 (2019)
DOI:10.1038/s41589-018-0185-2
  Sequence
Reference
2  [PMID:30664302]
  Authors
Lichman BR, O'Connor SE, Kries H
  Title
Biocatalytic Strategies towards [4+2] Cycloadditions.
  Journal
Chemistry 25:6864-6877 (2019)
DOI:10.1002/chem.201805412
Other DBs
ExplorEnz - The Enzyme Database: 5.5.1.34
IUBMB Enzyme Nomenclature: 5.5.1.34
ExPASy - ENZYME nomenclature database: 5.5.1.34
BRENDA, the Enzyme Database: 5.5.1.34

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