KEGG ENZYME: 5.6.1.4

ENZYME: 5.6.1.4

Entry

EC 5.6.1.4 Enzyme

Name

minus-end-directed kinesin ATPase;
non-claret disjunctional;
ncd (gene name)

Class

Isomerases;
Isomerases altering macromolecular conformation;
Enzymes altering polypeptide conformation or assembly

Sysname

kinesin ATP phosphohydrolase (minus-end-directed)

Reaction(IUBMB)

ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)

Substrate

ATP [CPD:C00002];
H2O [CPD:C00001];
kinesin associated with a microtubule at position n

Product

ADP [CPD:C00008];
phosphate [CPD:C00009];
kinesin associated with a microtubule at position n-1 (toward the minus end)

Comment

Kinesins are a family of motor proteins that move unidirectionally along microtubules as they hydrolyse ATP and are involved in organelle movement. This enzyme is similar to EC 5.6.1.3, plus-end-directed kinesin ATPase, but the organization of the different domains differs, resulting in movement in the opposite direction along the microtubules.

History

EC 5.6.1.4 created 2000, as 3.6.4.5, transferred 2018 to EC 5.6.1.4

Reference

1 [PMID:2261638]

Authors

McDonald HB, Stewart RJ, Goldstein LS

Title

The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor.

Journal

Cell 63:1159-65 (1990)
DOI:10.1016/0092-8674(90)90412-8

Sequence

[dme:Dmel_CG7831]

Reference

2 [PMID:8473343]

Authors

Chandra R, Salmon ED, Erickson HP, Lockhart A, Endow SA

Title

Structural and functional domains of the Drosophila ncd microtubule motor protein.

Journal

J Biol Chem 268:9005-13 (1993)

Sequence

[dme:Dmel_CG7831]

Reference

3 [PMID:8112290]

Authors

Lockhart A, Cross RA

Title

Origins of reversed directionality in the ncd molecular motor.

Journal

EMBO J 13:751-7 (1994)
DOI:10.1002/j.1460-2075.1994.tb06317.x

Sequence

[dme:Dmel_CG7831]

Reference

4 [PMID:9288974]

Authors

Henningsen U, Schliwa M.

Title

Reversal in the direction of movement of a molecular motor.

Journal

Nature 389:93-6 (1997)
DOI:10.1038/38022

Reference

5 [PMID:9796817]

Authors

Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ.

Title

Direction determination in the minus-end-directed kinesin motor ncd.

Journal

Nature 395:813-6 (1998)
DOI:10.1038/27463

Other DBs

ExplorEnz - The Enzyme Database:

5.6.1.4

IUBMB Enzyme Nomenclature:

5.6.1.4

ExPASy - ENZYME nomenclature database:

5.6.1.4

BRENDA, the Enzyme Database:

5.6.1.4

All links

Protein sequence (67)   
   UniProt (35)   
   RefSeq(pep) (32)   
DNA sequence (45)   
   RefSeq(nuc) (38)   
   GenBank (6)   
   EMBL (1)   
All databases (112)   

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