KEGG   ENZYME: 6.1.1.23Help
Entry
EC 6.1.1.23                 Enzyme                                 

Name
aspartate---tRNAAsn ligase;
nondiscriminating aspartyl-tRNA synthetase
Class
Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
BRITE hierarchy
Sysname
L-aspartate:tRNAAsx ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-aspartate + tRNAAsx = AMP + diphosphate + L-aspartyl-tRNAAsx [RN:R03647 R05577]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
L-aspartate [CPD:C00049];
tRNAAsx
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-aspartyl-tRNAAsx
Comment
When this enzyme acts on tRNAAsp, it catalyses the same reaction as EC 6.1.1.12, aspartate---tRNA ligase. It has, however, diminished discrimination, so that it can also form aspartyl-tRNAAsn. This relaxation of specificity has been found to result from the absence of a loop in the tRNA that specifically recognizes the third position of the anticodon [1]. This accounts for the ability of this enzyme in, for example, Thermus thermophilus, to recognize both tRNAAsp (GUC anticodon) and tRNAAsn (GUU anticodon). The aspartyl-tRNAAsn is not used in protein synthesis until it is converted by EC 6.3.5.6, asparaginyl-tRNA synthase (glutamine-hydrolysing), into asparaginyl-tRNAAsn.
History
EC 6.1.1.23 created 2002
Pathway
ec00970  Aminoacyl-tRNA biosynthesis
Orthology
K09759  nondiscriminating aspartyl-tRNA synthetase
Genes
BAN: BA_2186(aspS1)
BAR: GBAA_2186(aspS1)
BAT: BAS2031(aspC)
BAH: BAMEG_2410(aspS1)
BAI: BAA_2247(aspS1)
BCE: BC2169(aspC)
BCZ: BCE33L1981(aspS)
BCR: BCAH187_A2327(aspS)
BCB: BCB4264_A2191(aspS)
BCU: BCAH820_2205(aspS1)
BCG: BCG9842_B3128(aspS1)
BCQ: BCQ_2153(aspS-1)
BCX: BCA_2271(aspS1)
BTK: BT9727_1999(aspC)
BTL: BALH_1944(aspS)
CAC: CA_C2979(aspC) CA_C3564(aspC)
CPF: CPF_0770(aspS)
CBO: CBO1019(aspC)
CBA: CLB_1059(aspS-1)
CBH: CLC_1072(aspC)
CBY: CLM_1174(aspS)
CBL: CLK_0462(aspS)
CBK: CLL_A2506(aspS)
CBB: CLD_3547(aspS)
CBI: CLJ_B1064(aspS_1)
CBF: CLI_1101(aspS-1)
CBE: Cbei_2126
AMT: Amet_3390
RHO: RHOM_10080(aspC)
ROP: ROP_35810
BCV: Bcav_4171
NCA: Noca_1189
KFL: Kfla_5221
SRO: Sros_1870
AMD: AMED_1332(aspC)
MIL: ML5_3687
VMA: VAB18032_27436(aspC)
CAI: Caci_5912
SNA: Snas_4889
DRA: DR_1055(aspC)
DGE: Dgeo_0796
TRA: Trad_2070
TTH: TT_C1087
TTJ: TTHA1452(aspC)
MRB: Mrub_2287
PUV: PUV_24770(aspS-A)
MJA: MJ_1555
MMP: MMP1616(aspS)
MMD: GYY_08935(aspC)
MAE: Maeo_0028
MVO: Mvol_1504
MAC: MA_1684(aspS)
MBAR: MSBR2_0244
MBAK: MSBR3_0195
MMA: MM_0074
MMAC: MSMAC_2606
METM: MSMTP_1255
MTHE: MSTHC_2504
MTHR: MSTHT_0766
MHOR: MSHOH_2066
MBU: Mbur_0772
MMET: MCMEM_1059
MMH: Mmah_0849
MPY: Mpsy_0046
MTP: Mthe_0623
MCJ: MCON_1668(aspS)
MHI: Mhar_0069
MHU: Mhun_2446
MLA: Mlab_0039
MBG: BN140_1355(aspC1)
MEMA: MMAB1_1731(aspS)
MPI: Mpet_1892
MBN: Mboo_0105
MPL: Mpal_0276
MPD: MCP_0705(aspS)
MEZ: Mtc_1518(aspS)
RCI: RCIX42(aspS)
MTH: MTH_226
MMG: MTBMA_c06750(aspS)
METC: MTCT_0188
MWO: MWSIV6_0638(aspS)
MST: Msp_0135(aspS)
MSI: Msm_1236
MRU: mru_1014(aspS)
MEB: Abm4_0682(aspS)
MMIL: sm9_0043(aspS)
MEYE: TL18_10495(aspC)
MOL: YLM1_0558
METH: MBMB1_1065(aspS)
MFC: BRM9_1267(aspS)
MFI: DSM1535_1950(aspS)
MCUB: MCBB_0932(aspS)
MFV: Mfer_0591
MKA: MK0710(asnS)
AFU: AF_0920
FPL: Ferp_1526
GAC: GACE_1475
GAH: GAH_01056
HAL: VNG_0461G(aspS)
HSL: OE_1687F(aspS)
HHB: Hhub_1409(aspS)
HJE: HacjB3_13010(aspC)
HALH: HTSR_1476(aspC6)
HHSR: HSR6_1548(aspS)
HSU: HLASF_0582(aspS)
HSF: HLASA_0578(aspS)
HMA: rrnAC1657(aspS)
HHI: HAH_2198(aspS)
HHN: HISP_11200(aspC)
HAB: SG26_03570(aspC)
NPH: NP_1168A(aspS)
NMO: Nmlp_3492(aspS)
HUT: Huta_2727
HTI: HTIA_2475
HMU: Hmuk_3044
HWA: HQ_1270A(aspS)
HWC: Hqrw_1299(aspS)
HVO: HVO_0677(aspS)
HME: HFX_0640(aspS)
HGI: ABY42_03225(aspC)
HLA: Hlac_1657
HTU: Htur_2681
NMG: Nmag_1072(aspS)
NAT: NJ7G_1985
SALI: L593_04135(aspC)
HLR: HALLA_12915(aspC)
TAC: Ta0946
TVO: TVG1147365(TVG1147365)
PTO: PTO1214
FAI: FAD_0280(aspS)
CDIV: CPM_0581
MER: MMINT_11970(aspC)
MEAR: Mpt1_c07320(aspS2)
MARC: AR505_1150
ABI: Aboo_0465
APE: APE_2192.1(aspS)
ACJ: ACAM_1377(aspS)
SMR: Smar_0296
IHO: Igni_1435
IIS: EYM_06180(aspC)
DKA: DKAM_0911
TAG: Tagg_0704
IAG: Igag_0068
HBU: Hbut_0034
STO: STK_02050(aspS)
SSO: SSO0173(aspS)
SOL: Ssol_1151
SSOA: SULA_1187(aspC)
SSOL: SULB_1188(aspC)
SSOF: SULC_1186(aspC)
SAI: Saci_0768(aspS)
SACN: SacN8_03725(aspC)
SACR: SacRon12I_03710(aspC)
SACS: SUSAZ_03455(aspC)
SID: M164_1972
SII: LD85_2224
SIH: SiH_1909
SIR: SiRe_1837
SIC: SiL_1818
MSE: Msed_0205
MCN: Mcup_1868
AHO: Ahos_0632
PAI: PAE0703
PIS: Pisl_0679
PCL: Pcal_2119
PAS: Pars_2350
PYR: P186_2206
POG: Pogu_2516
TNE: Tneu_1987
CMA: Cmaq_0746
TUZ: TUZN_0620
TTN: TTX_0953(aspS)
VDI: Vdis_2301
VMO: VMUT_0615
TPE: Tpen_0628
ASC: ASAC_0458
NMR: Nmar_0871
NID: NPIRD3C_1327(aspS)
NIN: NADRNF5_1182(aspS)
NCT: NMSP_0847(aspS_2)
NGA: Ngar_c02730(aspS1)
NVN: NVIE_014140(aspS2)
NEV: NTE_02820
TAA: NMY3_02651(asnS_1)
NBV: T478_0773(aspS)
NDV: NDEV_1112(aspS)
NEQ: NEQ535
MARH: Mia14_0202
KCR: Kcr_1200
BARC: AOA65_0635(aspS)
 » show all
Taxonomy
Reference
1  [PMID:10966471]
  Authors
Ibba M, Soll D.
  Title
Aminoacyl-tRNA synthesis.
  Journal
Annu Rev Biochem 69:617-50 (2000)
DOI:10.1146/annurev.biochem.69.1.617
Reference
2  [PMID:9724658]
  Authors
Schmitt E, Moulinier L, Fujiwara S, Imanaka T, Thierry JC, Moras D.
  Title
Crystal structure of aspartyl-tRNA synthetase from Pyrococcus kodakaraensis KOD: archaeon specificity and catalytic mechanism of adenylate formation.
  Journal
EMBO J 17:5227-37 (1998)
DOI:10.1093/emboj/17.17.5227
Reference
3  [PMID:9789000]
  Authors
Becker HD, Kern D.
  Title
Thermus thermophilus: a link in evolution of the tRNA-dependent amino acid amidation pathways.
  Journal
Proc Natl Acad Sci U S A 95:12832-7 (1998)
DOI:10.1073/pnas.95.22.12832
Other DBs
ExplorEnz - The Enzyme Database: 6.1.1.23
IUBMB Enzyme Nomenclature: 6.1.1.23
ExPASy - ENZYME nomenclature database: 6.1.1.23
BRENDA, the Enzyme Database: 6.1.1.23
CAS: 9027-32-1

DBGET integrated database retrieval system