KEGG   ENZYME: 6.1.2.1
Entry
EC 6.1.2.1                  Enzyme                                 
Name
D-alanine---(R)-lactate ligase;
VanA;
VanB;
VanD
Class
Ligases;
Forming carbon-oxygen bonds;
acid-alcohol ligases (ester synthases)
Sysname
D-alanine:(R)-lactate ligase (ADP-forming)
Reaction(IUBMB)
D-alanine + (R)-lactate + ATP = D-alanyl-(R)-lactate + ADP + phosphate [RN:R09588]
Reaction(KEGG)
R09588
Substrate
D-alanine [CPD:C00133];
(R)-lactate [CPD:C00256];
ATP [CPD:C00002]
Product
D-alanyl-(R)-lactate [CPD:C19694];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
The product of this enzyme, the depsipeptide D-alanyl-(R)-lactate, can be incorporated into the peptidoglycan pentapeptide instead of the usual D-alanyl-D-alanine dipeptide, which is formed by EC 6.3.2.4, D-alanine---D-alanine ligase. The resulting peptidoglycan does not bind the glycopeptide antibiotics vancomycin and teicoplanin, conferring resistance on the bacteria.
History
EC 6.1.2.1 created 2010
Orthology
K15739  D-alanine---(R)-lactate ligase
Genes
ASEDIRT44_16970(vanA)
PLVERIC2_c07960(vanA)
PSONJI735_32490(vanA)
TVUAB849_010220(vanA)
LAHLA20533_00580
EFAEF2294(vanB)
EFCEFAU004_02775(vanB)
EFAUEFAU085_00735(vanB)
ESGEsVE80_p1-00080(vanA)
CARGRSJ17_20565
DSYDSY3690
DHDDhaf_1664
DDLDesdi_1473
FWADCMF_02700(vanB)
AMICAmi3637_13565(vanA)
LCGL3BBH23_19240(vanB)
NTPCRH09_30840(vanB)
NWLNWFMUON74_45700
TPRTpau_0523
TOYFO059_01075(vanA)
SCOSCO3595(vanB)
SLVSLIV_20320
SXISXIM_54620
STRCAA958_28375(vanB)
SLSSLINC_1844
SAQUEJC51_03785(vanA)
SBYH7H31_16175(vanA)
SRIMCP984_39910(vanA)
SSPBCP982_31320(vanA-Sc)
SROIIAG44_39585(vanA-Sc)
YIALO772_06590(vanA)
RTXTI83_01670(vanB)
RTCAPU90_04820(vanB)
RTNA6122_0299
RRYC1O28_00080(vanA)
RIAC7V51_10855(vanA)
AGGC1N71_08265
NAROCFH99_00255(vanB)
KFLKfla_3620
KQIF1D05_26435(vanA)
ACTWF7P10_09890(vanA)
FREFranean1_7137
AMDAMED_8289(ddlA)
AMNRAM_42565(vanB)
AMMAMES_8162(ddlA)
AMZB737_8163(ddlA)
AOIAORI_1472(ddl)
AJAAJAP_32170
AMYCCU254_05730(vanB)
AMYBBKN51_41395(vanB)
AABA4R43_21435(vanB)
AMYYYIM_36535(vanB)
AORISD37_33760(vanB)
SESPBN6_49760
SSYIEKG83_05355(vanA)
KALKALB_1172
KPHYAOZ06_08680(vanB) AOZ06_28865(vanB)
PSUUPsuf_054820
ATLAthai_15820
ASERAsera_58740
DVCDvina_26165(vanA)
CAICaci_5793
SNASnas_5356
GLYK3N28_22515(vanA)
CWOCwoe_1414
IAMHC251_07060
OLSOlsu_1201
TIOINP52_03930
KBSEPA93_09965(vanA)
 » show all
Reference
1  [PMID:1931965]
  Authors
Bugg TD, Wright GD, Dutka-Malen S, Arthur M, Courvalin P, Walsh CT
  Title
Molecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA.
  Journal
Biochemistry 30:10408-15 (1991)
DOI:10.1021/bi00107a007
  Sequence
Reference
2  [PMID:7957913]
  Authors
Meziane-Cherif D, Badet-Denisot MA, Evers S, Courvalin P, Badet B
  Title
Purification and characterization of the VanB ligase associated with type B vancomycin resistance in Enterococcus faecalis V583.
  Journal
FEBS Lett 354:140-2 (1994)
DOI:10.1016/0014-5793(94)01096-X
  Sequence
[efa:EF2294]
Reference
3  [PMID:9303405]
  Authors
Perichon B, Reynolds P, Courvalin P
  Title
VanD-type glycopeptide-resistant Enterococcus faecium BM4339.
  Journal
Antimicrob Agents Chemother 41:2016-8 (1997)
DOI:10.1128/AAC.41.9.2016
Other DBs
ExplorEnz - The Enzyme Database: 6.1.2.1
IUBMB Enzyme Nomenclature: 6.1.2.1
ExPASy - ENZYME nomenclature database: 6.1.2.1
BRENDA, the Enzyme Database: 6.1.2.1

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