KEGG   ENZYME: 6.1.2.1Help
Entry
EC 6.1.2.1                  Enzyme                                 

Name
D-alanine---(R)-lactate ligase;
VanA;
VanB;
VanD
Class
Ligases;
Forming carbon-oxygen bonds;
Acid-alcohol ligases (ester synthases)
BRITE hierarchy
Sysname
D-alanine:(R)-lactate ligase (ADP-forming)
Reaction(IUBMB)
D-alanine + (R)-lactate + ATP = D-alanyl-(R)-lactate + ADP + phosphate [RN:R09588]
Reaction(KEGG)
Substrate
D-alanine [CPD:C00133];
(R)-lactate [CPD:C00256];
ATP [CPD:C00002]
Product
D-alanyl-(R)-lactate [CPD:C19694];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
The product of this enzyme, the depsipeptide D-alanyl-(R)-lactate, can be incorporated into the peptidoglycan pentapeptide instead of the usual D-alanyl-D-alanine dipeptide, which is formed by EC 6.3.2.4, D-alanine---D-alanine ligase. The resulting peptidoglycan does not bind the glycopeptide antibiotics vancomycin and teicoplanin, conferring resistance on the bacteria.
History
EC 6.1.2.1 created 2010
Orthology
K15739  D-alanine---(R)-lactate ligase
Genes
PLV: ERIC2_c07960(vanA)
LAH: LA20533_00580
EFA: EF2294(vanB)
EFC: EFAU004_02775(vanB)
EFAU: EFAU085_00735(vanB)
CARG: RSJ17_20565
DSY: DSY3690
DHD: Dhaf_1664
DDL: Desdi_1473
NTP: CRH09_30840(vanB)
TPR: Tpau_0523
SCO: SCO3595(vanB)
STRC: AA958_28375(vanB)
RTX: TI83_01670(vanB)
RTC: APU90_04820(vanB)
KFL: Kfla_3620
AMD: AMED_8289(ddlA)
AMN: RAM_42565(vanB)
AMM: AMES_8162(ddlA)
AMZ: B737_8163(ddlA)
AOI: AORI_1472(ddl)
AMYC: CU254_05730(vanB)
AMYB: BKN51_41395(vanB)
SESP: BN6_49760
KAL: KALB_1172
KPHY: AOZ06_08680(vanB) AOZ06_28865(vanB)
CAI: Caci_5793
SNA: Snas_5356
CWO: Cwoe_1414
OLS: Olsu_1201
 » show all
Taxonomy
Reference
1  [PMID:1931965]
  Authors
Bugg TD, Wright GD, Dutka-Malen S, Arthur M, Courvalin P, Walsh CT
  Title
Molecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA.
  Journal
Biochemistry 30:10408-15 (1991)
  Sequence
Reference
2  [PMID:7957913]
  Authors
Meziane-Cherif D, Badet-Denisot MA, Evers S, Courvalin P, Badet B
  Title
Purification and characterization of the VanB ligase associated with type B vancomycin resistance in Enterococcus faecalis V583.
  Journal
FEBS Lett 354:140-2 (1994)
DOI:10.1016/0014-5793(94)01096-X
  Sequence
[efa:EF2294]
Reference
3  [PMID:9303405]
  Authors
Perichon B, Reynolds P, Courvalin P
  Title
VanD-type glycopeptide-resistant Enterococcus faecium BM4339.
  Journal
Antimicrob Agents Chemother 41:2016-8 (1997)
Other DBs
ExplorEnz - The Enzyme Database: 6.1.2.1
IUBMB Enzyme Nomenclature: 6.1.2.1
ExPASy - ENZYME nomenclature database: 6.1.2.1
BRENDA, the Enzyme Database: 6.1.2.1

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