| Entry |
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| Name |
[butirosin acyl-carrier protein]---L-glutamate ligase;
[BtrI acyl-carrier protein]---L-glutamate ligase;
BtrJ
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| Class |
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
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| Sysname |
[BtrI acyl-carrier protein]:L-glutamate ligase (ADP-forming)
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| Reaction(IUBMB) |
(1) ATP + L-glutamate + BtrI acyl-carrier protein = ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein] [RN: R08905];
(2) ATP + L-glutamate + 4-amino butanoyl-[BtrI acyl-carrier protein] = ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] [RN: R08906]
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| Reaction(KEGG) |
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| Substrate |
ATP [CPD: C00002];
L-glutamate [CPD: C00025];
BtrI acyl-carrier protein;
4-amino butanoyl-[BtrI acyl-carrier protein]
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| Product |
ADP [CPD: C00008];
phosphate [CPD: C00009];
L-glutamyl-[BtrI acyl-carrier protein];
4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
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| Comment |
Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L-glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC 4.1.1.95, L-glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L-glutamate molecule.
Requires Mg2+ or Mn2+, and activity is enhanced in the presence of Mn2+.
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| History |
EC 6.2.1.39 created 2012
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| Pathway |
| ec00524 | Neomycin, kanamycin and gentamicin biosynthesis |
| ec01130 | Biosynthesis of antibiotics |
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| Orthology |
| K13559 | [BtrI acyl-carrier protein]--L-glutamate ligase |
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| Reference |
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| Authors |
Li Y, Llewellyn NM, Giri R, Huang F, Spencer JB |
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| Title |
Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 6.2.1.39 |
| ExPASy - ENZYME nomenclature database: | 6.2.1.39 |
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