KEGG   ENZYME: 6.2.1.41
Entry
EC 6.2.1.41                 Enzyme                                 
Name
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate---CoA ligase;
fadD3 (gene name);
HIP---CoA ligase
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate:CoA ligase (AMP-forming)
Reaction(IUBMB)
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA = AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA [RN:R10769]
Reaction(KEGG)
R10769
Substrate
ATP [CPD:C00002];
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate [CPD:C20199];
CoA [CPD:C00010]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA [CPD:C20838]
Comment
The enzyme, characterized from actinobacterium Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol and cholate. The enzyme is very specific for its substrate, and requires that the side chain at C17 is completely removed.
History
EC 6.2.1.41 created 2014
Pathway
ec00984  Steroid degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K18687  HIP---CoA ligase
Genes
RGLCS053_01725
VAFD1115_23235
PREPCA10_43630
PFUWKF707C_11910
PPUTL483_14110
PPUNPP4_27170
PPUUPputUW4_01676
PKCPKB_4307 PKB_4315
PFZAV641_10585
PLQAA042_20385
PPSYAOC04_07885
PSETTHL1_4405
PSILPMA3_11755
PKEDLD99_14685
PUMHGP31_20740
PPSHG5J76_13540
PLALFXN65_21115
PKHJLK41_05755
PAZEKSS91_19215
PPHABVH74_17915
PTAENCTC10697_02067(fadD_1)
PCAVD3880_17445
PPHNHU825_14705
PIZLAB08_R40810
SDEGGOM96_01710
HARRHV822_17930
SPLSpea_2126
SHLShal_2098
CBERB5D82_11310
PHAPSHAa2132
PTNPTRA_a2585
PNGPNIG_a2779
SNANI6N98_06920
ZALAZF00_05245
HALCEY643_14275
AFUSEYZ66_12945
KAKKalk_07825
MSECLN244_04845
MRZKDW95_00840
ENWMJO57_21325
SDFACG33_03745 ACG33_09100
SOKD0B54_05255
SINIGT972_08475
FCEJN531_005465
GPBHDN1F_27370
RINACS15_4389
REHH16_B0644(h16_B0644)
CNCCNE_2c05910(yngI) CNE_BB1p00170
CUHBJN34_30920
REUReut_A1547
CTIRALTA_B0493
CBWRR42_s2339
COXE0W60_08165
BURBcep18194_B1417
BCJBCAM1643
BCENDM39_6207
BCEOI35_5503
BCTGEM_4013 GEM_4107
BCEDDM42_6315
BCEPAPZ15_30840
BPYRABD05_27660
BCONNL30_03970
BDFWI26_21165 WI26_21350
BTEIWS51_05840
BPSLWS57_05870
BMECWJ16_26950
BSTLBBJ41_21505
BANNJFN94_25670 JFN94_26150
BARINLX30_30005
BURKDM992_32475
BFNOI25_4610
PVEUC34_06910
CABASBC2_48800(fadD3_3)
CTTCtCNB1_1360
CTESO987_07775
DIHG7047_09740
DOEDENOEST_1378(fadD)
AZDCDA09_09375
THUAC731_008835
AAKAA2016_1500 AA2016_3673
NTDEGO55_13795
CAENK5X80_14620
TSVDSM104635_02997(lcfB_4)
NARSaro_3767
NPPPP1Y_Mpl2929
NPNJI59_09720
NOTC7W88_20835
NGFFRF71_13155 FRF71_13260
SWISwit_0310 Swit_0384 Swit_2105 Swit_3306
SPHDHY78_09020 HY78_14990 HY78_26180 HY78_26565
RDICMV14_05515 CMV14_05925
SPHMG432_04705
SSANNX02_14695
SPKCKC8_01165
SPHAD3Y57_15520
SLANGV829_01960
SBINSBA_ch2_4510(fadD3)
SCHSphch_3617 Sphch_3631
SYBTZ53_05605 TZ53_05675
SYAA6768_12225 A6768_12295
SUFLFIL70_22020 FIL70_22090
SBARH5V43_18870 H5V43_18940
PHAOHF685_05620
PHZCHX26_05220
NAOY958_14665
MTURv3561(fadD3)
MTVRVBD_3561
MTCMT3666
MRAMRA_3600(fadD3)
MTFTBFG_13594
MTBTBMG_03600
MTKTBSG_03627
MTZTBXG_003576
MTGMRGA327_21990
MTIMRGA423_22505
MTECCDC5079_3302
MTURCFBS_3777(fadD3)
MTLCCDC5180_3254
MTOMTCTRI2_3625(fadD3)
MTDUDA_3561(fadD3)
MTNERDMAN_3906(fadD3)
MTJJ112_19175
MTUBMT7199_3623
MTUEJ114_19045
MTXM943_18305
MTUHI917_24990
MTULTBHG_03501
MTUTHKBT1_3762(fadD3)
MTUUHKBT2_3771(fadD3)
MTQHKBS1_3774(fadD3)
MBOBQ2027_MB3591(fadD3)
MBBBCG_3625(fadD3)
MBTJTY_3626(fadD3)
MBMBCGMEX_3623(fadD3)
MBKK60_036990
MBXBCGT_3427
MAFMAF_35730(fadD3)
MMICRN08_3938
MCEMCAN_35721(fadD3)
MCQBN44_110053(fadD)
MCVBN43_90059(fadD)
MCXBN42_90058(fadD)
MCZBN45_100060(fadD)
MPAMAP_0506c(fadD3) MAP_0780(fadD3)
MAOMAP4_3079 MAP4_3361
MAVIRC58_15265 RC58_16670
MAVURE97_15295 RE97_16705
MAVMAV_0600(fadD) MAV_0971
MITOCO_05090 OCO_08360
MIAOCU_05130 OCU_08440
MIDMIP_00953 MIP_01417
MYOOEM_05150 OEM_08450
MCHIAN480_02540 AN480_05365
MIROCQ_05240 OCQ_08520
MMALCKJ54_02480 CKJ54_04170
MLPMLM_0744
MMANMMAN_48040(fadD3) MMAN_51360
MSAMycsm_04438 Mycsm_05824
MULMUL_4126(fadD3)
MMIMMAR_4688(fadD3_2) MMAR_5050(fadD3)
MMAEMMARE11_45140(fadD3_2) MMARE11_48650(fadD3)
MLIMULP_05308(fadD3)
MPSEMPSD_48500 MPSD_52500(fadD3)
MSHOMSHO_05320(fadD3)
MMCMmcs_3655 Mmcs_4694
MKMMkms_3728 Mkms_4780
MJLMjls_3668 Mjls_5079
MMMW7S_02485 W7S_04130
MKNMKAN_09685 MKAN_12040
MYVG155_20365 G155_26340
MYEAB431_21360 AB431_26505
MGOAFA91_10715 AFA91_16715
MHADB586_04505
MDXBTO20_04540 BTO20_10895
MSHGMSG_04223 MSG_04547(fadD3)
MFJMFLOJ_40780(fadD3) MFLOJ_44090
MSTOMSTO_44750(fadD3) MSTO_48100
MSIMMSIM_29200(fadD3) MSIM_32550
MSAKMSAS_25700 MSAS_29040(fadD3)
MKUI2456_22325 I2456_25635
MGROFZ046_02880 FZ046_24070
MXEMYXE_05150(fadD3) MYXE_11440
MNVMNVI_09050 MNVI_12420(fadD3)
MPAGC0J29_25770 C0J29_27710
MNMMNVM_23560(fadD3) MNVM_26540
MGORH0P51_24020 H0P51_25765
MCOOMCOO_23770 MCOO_27190(fadD3)
MBAIMB901379_04092(lcfB_14) MB901379_04440(lcfB_16)
MSEOMSEO_31260 MSEO_34450(fadD3)
MHEKJMUB5695_00476(fadD3) JMUB5695_00690 JMUB5695_01061
MGAUMGALJ_25030 MGALJ_38370(fadD3)
MLJMLAC_47430(fadD3)
MBRDMBRA_20090(fadD3) MBRA_24120 MBRA_50720
MSHJMSHI_38740(fadD3)
MKRMKOR_35470(fadD3)
MDFK0O62_20330 K0O62_25855(fadD3)
MMAMK3U93_03775 K3U93_22640(fadD3)
MHOLK3U96_03160(fadD3) K3U96_08810
MHERK3U94_19405 K3U94_20970(fadD3)
MSENK3U95_09565 K3U95_25370(fadD3)
MPAEK0O64_20895 K0O64_26000(fadD3)
MSPGF6B93_20445
MOTLTS72_01860 LTS72_27960(fadD3)
MVMMJO54_19365 MJO54_20865(fadD3)
MRFMJO55_18665 MJO55_24065(fadD3)
MPAAMKK62_02450(fadD3) MKK62_04030
MCROMI149_21470 MI149_26310(fadD3)
MSMMSMEG_4772
MSGMSMEI_4650 MSMEI_5853(fadD3)
MSBLJ00_23605 LJ00_29730
MSNLI99_23610 LI99_29735
MSHLI98_23615 LI98_29740
MVAMvan_4133 Mvan_5286
MGIMflv_1480 Mflv_2525
MSPMspyr1_19520 Mspyr1_46870
MCBMycch_3565 Mycch_4656
MNED174_24625
MYNMyAD_24160
MFTXA26_41550 XA26_53340
MPHLMPHLCCUG_03698(lcfB_8)
MVQMYVA_3927 MYVA_5196
MLLB1R94_25345
MRHMycrhN_2265 MycrhN_4054
MTHN4412656_02988(fadD_12) 4412656_04084(fadD_18)
MHASMHAS_01072(fadD3_4) MHAS_03765(fadD3_6)
MDUMDUV_05460 MDUV_18420 MDUV_49150(fadD3)
MCHTMCHIJ_19460(fadD3) MCHIJ_29620
MAUUNCTC10437_03867(fadD_9) NCTC10437_05044(fadD_13)
MMAGMMAD_38850 MMAD_48440(fadD3)
MMORMMOR_11950(fadD3) MMOR_24330
MFXMFAL_01670 MFAL_32670(fadD3)
MAICMAIC_05290(fadD3) MAIC_37640
MIJMINS_00620(fadD3) MINS_34900
MALVMALV_42360(fadD3) MALV_53670
MTYMTOK_22130(fadD3)
MPSCMPSYJ_15990(fadD3) MPSYJ_26800
MARZMARA_47560(fadD3) MARA_58560
MGADMGAD_37450(fadD3) MGAD_57520
MHEVMHEL_04750 MHEL_27740(fadD3)
MSARMSAR_10910(fadD3) MSAR_47980
MANYMANY_13600 MANY_32920(fadD3)
MAUBMAUB_12420 MAUB_58040
MPOFMPOR_42530 MPOR_53740(fadD3)
MMUCC1S78_018185
MMATMMAGJ_00400 MMAGJ_34150(fadD3)
MBOKMBOE_04690(fadD3) MBOE_32280
MFGK6L26_05485(fadD3) K6L26_11395
MSEIMSEDJ_02390(fadD3) MSEDJ_50120
MFLVNCTC10271_00480(fadD_1) NCTC10271_01606(fadD_7)
MCEEMCEL_00580 MCEL_37860(fadD3)
MMONEWR22_18865 EWR22_25395
MABMAB_0596c
MMVMYCMA_0305
MABBMASS_0566
MABLMMASJCM_0585
MCHEBB28_02845
MIZBAB75_03595
MSTEMSTE_00554
MSAOMYCSP_02550
MSALDSM43276_00514
MJDJDM601_3424(fadD3_2) JDM601_3717(fadD3)
MTER4434518_03408(fadD3_2) 4434518_03719(fadD3)
MMINMMIN_12410(fadD3)
MHIBMHIB_28310 MHIB_31060(fadD3)
ASDAS9A_0944
MPAKMIU77_02035(fadD3) MIU77_10645
NFANFA_4830
NFRERS450000_03753(fadD_8)
NCYNOCYR_0513
NBRO3I_002775
NNONONO_c05120 NONO_c41750 NONO_c58970
NSLBOX37_02240 BOX37_18195
NSRNS506_01276(acsL) NS506_06831
NTPCRH09_03385
NOZDMB37_23675
NODFOH10_22725 FOH10_28625
NAHF5544_02710
NADNCTC11293_01257(fadD_1)
NWLNWFMUON74_05070 NWFMUON74_28700
NIEKV110_03105
NTCKHQ06_03580 KHQ06_35310
NHUH0264_03915 H0264_34430
NYALTV02_28805 LTV02_38505
NGPLTT66_15515 LTT66_27885 LTT66_28685
RHARHA1_ro04595
RERRER_08900
REYO5Y_03940
REBXU06_04180
RQIC1M55_04410
ROPROP_45400
ROAPd630_LPD01072
RPYY013_19130
RBYCEJ39_05495
RAVAAT18_04025
RHWBFN03_15060
RRZCS378_19670
RHQIM25_08980
RHODAOT96_14810
RRT4535765_00602(fadD_1) 4535765_04305(fadK_8)
RCRNCTC10994_02118(fadD_6) NCTC10994_03067(fadD_13)
RTMG4H71_07295
RKOJWS14_25305(fadD3)
RGOKYT97_13825 KYT97_23455 KYT97_24215
ROZCBI38_04305 CBI38_05550
RPSKJWS13_19230
RGORNMQ04_02615
REQREQ_07020
GBRGbro_3996
GPOGPOL_c43210
GORKTR9_3925(fadD3)
GOQACH46_17650
GTABCM27_20570
GITC6V83_03810
GRUGCWB2_20060(lcfB19)
GOMD7316_02344(fadD3_4) D7316_04486(fadD3_6)
GAVC5O27_08040
GODGKZ92_18990
GAMGII34_18975
GPDGII33_03785
GJIH1R19_18915
GOILK459_13625
GHNMVF96_19510
GAMIIHQ52_23040
GMGNWF22_06475
SPINKV203_08665 KV203_17315
TPRTpau_3836
TSMASU32_21855
TPULTPB0596_41760
DTMBJL86_0717
DITC3V38_09320
DIZCT688_03240
DPCA6048_03185
DLUA6035_03480
DKNNHB83_03040
TOYFO059_13900
SMASAVERM_3806(fadD16)
SGRSGR_3014
SGBWQO_20655
SCBSCAB_51911
SVLStrvi_0995
SRCM271_20830 M271_44415
SMALSMALA_4570
SSOII1A49_26910
SBHSBI_06310
SVESVEN_3097
SDVBN159_5012(fadD16)
SFISFUL_4340
SCIB446_16925
SVTSVTN_16190 SVTN_35955
STREGZL_01222 GZL_05169
SLDT261_4786
SLCSL103_02730
STRMM444_15600
SPRISPRI_4275
SCZABE83_14545
SCXAS200_26045
SRWTUE45_04305(lcfB_5)
SCLFBB341_11710
SGSAVL59_44295
STSIA4E84_17385
SALWCP975_15095
SGMGCM10017557_35940(fadD3)
SCADDN051_22275
SPHWNFX46_37245
SLSSLINC_3721
SNRSNOUR_23725
SALUDC74_3724 DC74_7422
SALLSAZ_19795 SAZ_38315
SPLULK06_018885
SAUOBV401_26700
SSIAA7J05_20260
SVUB1H20_20855
SPUNBFF78_24005
SGVB1H19_18045
SLAUSLA_3151
SALFSMD44_03682
SALJSMD11_3940
SLXSLAV_16680(lcfB3) SLAV_27185(fadD)
STROSTRMOE7_17095
SFKKY5_3519c
SNZDC008_14850
SGEDWG14_04793(fadD3_2)
SRJSRO_4198
SLKSLUN_17115
SDXC4B68_22860
SGDELQ87_22140
SQZFQU76_13315
SCYAEJ357_19865
STIRDDW44_13080
SKACP970_24690
SGZC0216_28150
SVNCP980_19310
SNKCP967_03170
SHAWCEB94_17835
SRKFGW37_13720
SFICEIZ62_18905
SGALCP966_15740
SSPODDQ41_12980
SFYGFH48_23150 GFH48_36350
SAQUEJC51_22160
SGFHEP81_04370
SCAVCVT27_20050
SBYH7H31_00530
SRIMCP984_22865
SSUBCP968_19240
SPHVF9278_20570
STSUB7R87_12920
SCHACP983_24260
SGXH4W23_17480 H4W23_38580
SCOECP976_19305
SSPBCP982_19280
SNFJYK04_03879(fadD3)
SPLACP981_16825
SBROGQF42_20345
STUIGCM10017668_30020
SATAC5746_24045
SHUNDWB77_04404(fadD3_2)
SCYGS1361_17585(lcfB6)
SFEUIM697_04450
SDWK7C20_14855 K7C20_16105
SCALI6J39_20675
SAUHSU9_014305
SPEUCGZ69_15455
SROIIAG44_23445
SXNIAG42_15145 IAG42_26605
SHKJ2N69_15530
SANUK7396_20725
STRYEQG64_20815
SGOBtest1122_09880
SINEKI385_19910
SLFJEQ17_20445
SDECL3078_19850
SAOVG3H79_21015
SDURM4V62_24190
SYANNRK68_14635
SRUGF0345_00695
SAKBK1J60_24945
STAALDH80_23025 LDH80_36450
SCTSCAT_2351
SCYSCATT_23360
KABB7C62_21185
STRIC7M71_000480
YIALO772_06015 LO772_06250 LO772_20310 LO772_32330
STRHGXP74_36245
MRNK8F61_15875
ARLAFL94_17015
ARYATC04_01260
ASUNKG104_08915
GPRJQN66_16930
PAEYKUF55_17940
NCANoca_2768
NDKI601_0619 I601_0635(lcfB_1)
NBEBack2_27300(fadD3)
NANOG5V58_09820
NAROCFH99_13485
NPIG7071_15905
NPCKUV85_04315
PSIMKR76_25120 KR76_25165
AEBC6I20_03325
NGVCDO52_09610
TCUTcur_3475
ACTWF7P10_03615 F7P10_29035 F7P10_38160
SROSros_2101
NOABKM31_34195
NCXNocox_09885(lcfB4)
NGNLCN96_11175
FREFranean1_4311
FRIFraEuI1c_4763
AMDAMED_3385
AMNRAM_17220
AMMAMES_3346
AMZB737_3346
AOIAORI_2499
AJAAJAP_26330
AMQAMETH_1197 AMETH_4459
AMYCCU254_23990
AMYBBKN51_05105
AABA4R43_16000
AMYYYIM_33170(lcfB8)
AORISD37_13550
AACDLWP59_29160
APRTMUY14_18565
AROONQK81_09855
ATHML1857_25360
PSEEFRP1_11590
PSEQAD006_19380
PECQAD017_27220
PHHAFB00_08730
PAUTPdca_08270(fadD3)
KALKALB_5110
KPHYAOZ06_42705
LEDBBK82_38875
ALOCRK57775
PMADBAY61_15980 BAY61_16235
SACEGIY23_13320
STPStrop_2660
SAQSare_2861
MICBMicB006_5685
AMSAMIS_19270
AIHAiant_18740
PSUUPsuf_059150 Psuf_059160
DVCDvina_21455
DFUDfulv_25890
DROSDrose_12930
DAURDaura_27560
DMATDmats_17090
CAICaci_8762
ABAIIMCC26256_111347
CWOCwoe_2360
IAMHC251_08970
 » show all
Reference
1  [PMID:16891113]
  Authors
Horinouchi M, Hayashi T, Koshino H, Kudo T
  Title
ORF18-disrupted mutant of Comamonas testosteroni TA441 accumulates significant amounts of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid and its derivatives after incubation with steroids.
  Journal
J Steroid Biochem Mol Biol 101:78-84 (2006)
DOI:10.1016/j.jsbmb.2006.06.006
  Sequence
Reference
2  [PMID:23146019]
  Authors
Casabon I, Crowe AM, Liu J, Eltis LD
  Title
FadD3 is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria.
  Journal
Mol Microbiol 87:269-83 (2013)
DOI:10.1111/mmi.12095
  Sequence
[mtu:Rv3561]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.41
IUBMB Enzyme Nomenclature: 6.2.1.41
ExPASy - ENZYME nomenclature database: 6.2.1.41
BRENDA, the Enzyme Database: 6.2.1.41

DBGET integrated database retrieval system