KEGG   ENZYME: 6.2.1.41
Entry
EC 6.2.1.41                 Enzyme                                 

Name
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate---CoA ligase;
fadD3 (gene name);
HIP---CoA ligase
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate:CoA ligase (AMP-forming)
Reaction(IUBMB)
ATP + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate + CoA = AMP + diphosphate + 3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA [RN:R10769]
Reaction(KEGG)
R10769
Substrate
ATP [CPD:C00002];
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate [CPD:C20199];
CoA [CPD:C00010]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoyl-CoA [CPD:C20838]
Comment
The enzyme, characterized from actinobacterium Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol and cholate. The enzyme is very specific for its substrate, and requires that the side chain at C17 is completely removed.
History
EC 6.2.1.41 created 2014
Pathway
ec00984  Steroid degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K18687  HIP---CoA ligase
Genes
RGL: CS053_01725
VAF: D1115_23235
PRE: PCA10_43630
PFUW: KF707C_11910
PPUT: L483_14110
PPUN: PP4_27170
PPUU: PputUW4_01676
PKC: PKB_4307 PKB_4315
PFZ: AV641_10585
PLQ: AA042_20385
PSET: THL1_4405
PSIL: PMA3_11755
SPL: Spea_2126
SHL: Shal_2098
PHA: PSHAa2132
REH: H16_B0644(h16_B0644)
BCJ: BCAM1643
BCEN: DM39_6207
BCEO: I35_5503
BCED: DM42_6315
BCON: NL30_03970
BTEI: WS51_05840
BPSL: WS57_05870
BMEC: WJ16_26950
BFN: OI25_4610
CABA: SBC2_48800(fadD3_3)
CTES: O987_07775
DOE: DENOEST_1378(fadD)
TSV: DSM104635_02997(lcfB_4)
NAR: Saro_3767
SPHM: G432_04705
SSAN: NX02_14695
SPKC: KC8_01165
MTU: Rv3561(fadD3)
MTV: RVBD_3561
MTC: MT3666
MRA: MRA_3600(fadD3)
MTUR: CFBS_3777(fadD3)
MTO: MTCTRI2_3625(fadD3)
MTD: UDA_3561(fadD3)
MTN: ERDMAN_3906(fadD3)
MTUE: J114_19045
MTUH: I917_24990
MTUL: TBHG_03501
MTUT: HKBT1_3762(fadD3)
MTUU: HKBT2_3771(fadD3)
MTQ: HKBS1_3774(fadD3)
MBO: BQ2027_MB3591(fadD3)
MBB: BCG_3625(fadD3)
MBT: JTY_3626(fadD3)
MBM: BCGMEX_3623(fadD3)
MBX: BCGT_3427
MAF: MAF_35730(fadD3)
MMIC: RN08_3938
MCE: MCAN_35721(fadD3)
MCQ: BN44_110053(fadD)
MCV: BN43_90059(fadD)
MCX: BN42_90058(fadD)
MCZ: BN45_100060(fadD)
MPA: MAP_0506c(fadD3) MAP_0780(fadD3)
MAV: MAV_0600(fadD) MAV_0971
MLP: MLM_0744
MMAN: MMAN_48040(fadD3) MMAN_51360
MUL: MUL_4126(fadD3)
MMI: MMAR_4688(fadD3_2) MMAR_5050(fadD3)
MMAE: MMARE11_45140(fadD3_2) MMARE11_48650(fadD3)
MLI: MULP_05308(fadD3)
MHAD: B586_04505
MSHG: MSG_04223 MSG_04547(fadD3)
MSTO: MSTO_44750(fadD3) MSTO_48100
MSIM: MSIM_29200(fadD3) MSIM_32550
MSAK: MSAS_25700 MSAS_29040(fadD3)
MXE: MYXE_05150(fadD3) MYXE_11440
MNV: MNVI_09050 MNVI_12420(fadD3)
MNM: MNVM_23560(fadD3) MNVM_26540
MCOO: MCOO_23770 MCOO_27190(fadD3)
MBAI: MB901379_04092(lcfB_14) MB901379_04440(lcfB_16)
MSEO: MSEO_31260 MSEO_34450(fadD3)
MPSE: MPSD_48500 MPSD_52500(fadD3)
MSHO: MSHO_05320(fadD3)
MGAU: MGALJ_25030 MGALJ_38370(fadD3)
MLJ: MLAC_47430(fadD3)
MSHJ: MSHI_38740(fadD3)
MKR: MKOR_35470(fadD3)
MMAM: K3U93_03775 K3U93_22640(fadD3)
MHOL: K3U96_03160(fadD3) K3U96_08810
MHER: K3U94_19405 K3U94_20970(fadD3)
MSEN: K3U95_09565 K3U95_25370(fadD3)
MPAE: K0O64_20895 K0O64_26000(fadD3)
MSG: MSMEI_4650 MSMEI_5853(fadD3)
MPHL: MPHLCCUG_03698(lcfB_8)
MTHN: 4412656_02988(fadD_12) 4412656_04084(fadD_18)
MHAS: MHAS_01072(fadD3_4) MHAS_03765(fadD3_6)
MCHT: MCHIJ_19460(fadD3) MCHIJ_29620
MAUU: NCTC10437_03867(fadD_9) NCTC10437_05044(fadD_13)
MMAG: MMAD_38850 MMAD_48440(fadD3)
MMOR: MMOR_11950(fadD3) MMOR_24330
MFX: MFAL_01670 MFAL_32670(fadD3)
MAIC: MAIC_05290(fadD3) MAIC_37640
MIJ: MINS_00620(fadD3) MINS_34900
MALV: MALV_42360(fadD3) MALV_53670
MTY: MTOK_22130(fadD3)
MPSC: MPSYJ_15990(fadD3) MPSYJ_26800
MARZ: MARA_47560(fadD3) MARA_58560
MGAD: MGAD_37450(fadD3) MGAD_57520
MHEV: MHEL_04750 MHEL_27740(fadD3)
MSAR: MSAR_10910(fadD3) MSAR_47980
MANY: MANY_13600 MANY_32920(fadD3)
MPOF: MPOR_42530 MPOR_53740(fadD3)
MMAT: MMAGJ_00400 MMAGJ_34150(fadD3)
MBOK: MBOE_04690(fadD3) MBOE_32280
MSEI: MSEDJ_02390(fadD3) MSEDJ_50120
MAB: MAB_0596c
MABB: MASS_0566
MCHE: BB28_02845
MSTE: MSTE_00554
MJD: JDM601_3424(fadD3_2) JDM601_3717(fadD3)
MTER: 4434518_03408(fadD3_2) 4434518_03719(fadD3)
MMIN: MMIN_12410(fadD3)
MHIB: MHIB_28310 MHIB_31060(fadD3)
ASD: AS9A_0944
NFA: NFA_4830
NFR: ERS450000_03753(fadD_8)
NAD: NCTC11293_01257(fadD_1)
RER: RER_08900
REY: O5Y_03940
ROP: ROP_45400
REQ: REQ_07020
RRT: 4535765_00602(fadD_1) 4535765_04305(fadK_8)
RCR: NCTC10994_02118(fadD_6) NCTC10994_03067(fadD_13)
RKO: JWS14_25305(fadD3)
GBR: Gbro_3996
GOR: KTR9_3925(fadD3)
GRU: GCWB2_20060(lcfB19)
GOM: D7316_02344(fadD3_4) D7316_04486(fadD3_6)
TPR: Tpau_3836
SMA: SAVERM_3806(fadD16)
SGR: SGR_3014
SGB: WQO_20655
SMAL: SMALA_4570
SCT: SCAT_2351
SBH: SBI_06310
SVE: SVEN_3097
SDV: BN159_5012(fadD16)
SFI: SFUL_4340
SLD: T261_4786
STRM: M444_15600
SPRI: SPRI_4275
SRW: TUE45_04305(lcfB_5)
SGM: GCM10017557_35940(fadD3)
SLAU: SLA_3151
SALJ: SMD11_3940
SLX: SLAV_16680(lcfB3) SLAV_27185(fadD)
SFK: KY5_3519c
SGE: DWG14_04793(fadD3_2)
SRJ: SRO_4198
SNF: JYK04_03879(fadD3)
SHUN: DWB77_04404(fadD3_2)
SCYG: S1361_17585(lcfB6)
SAUH: SU9_014305
NCA: Noca_2768
NDK: I601_0619 I601_0635(lcfB_1)
NBE: Back2_27300(fadD3)
TCU: Tcur_3475
SRO: Sros_2101
NCX: Nocox_09885(lcfB4)
AMD: AMED_3385
AMN: RAM_17220
AMM: AMES_3346
AMZ: B737_3346
AOI: AORI_2499
AMYY: YIM_33170(lcfB8)
AORI: SD37_13550
PSEE: FRP1_11590
PAUT: Pdca_08270(fadD3)
KAL: KALB_5110
ALO: CRK57775
SAQ: Sare_2861
CAI: Caci_8762
CWO: Cwoe_2360
 » show all
Reference
1  [PMID:16891113]
  Authors
Horinouchi M, Hayashi T, Koshino H, Kudo T
  Title
ORF18-disrupted mutant of Comamonas testosteroni TA441 accumulates significant amounts of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid and its derivatives after incubation with steroids.
  Journal
J Steroid Biochem Mol Biol 101:78-84 (2006)
DOI:10.1016/j.jsbmb.2006.06.006
  Sequence
Reference
2  [PMID:23146019]
  Authors
Casabon I, Crowe AM, Liu J, Eltis LD
  Title
FadD3 is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria.
  Journal
Mol Microbiol 87:269-83 (2013)
DOI:10.1111/mmi.12095
  Sequence
[mtu:Rv3561]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.41
IUBMB Enzyme Nomenclature: 6.2.1.41
ExPASy - ENZYME nomenclature database: 6.2.1.41
BRENDA, the Enzyme Database: 6.2.1.41

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