KEGG   ENZYME: 6.2.1.42
Entry
EC 6.2.1.42                 Enzyme                                 

Name
3-oxocholest-4-en-26-oate---CoA ligase;
fadD19 (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
(25S)-3-oxocholest-4-en-26-oate:CoA ligase (AMP-forming)
Reaction(IUBMB)
ATP + (25S)-3-oxocholest-4-en-26-oate + CoA = AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA [RN:R10770]
Reaction(KEGG)
R10770
Substrate
ATP [CPD:C00002];
(25S)-3-oxocholest-4-en-26-oate [CPD:C21076];
CoA [CPD:C00010]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
(25S)-3-oxocholest-4-en-26-oyl-CoA [CPD:C20840]
Comment
The enzyme, characterized from actinobacterium Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol. It is responsible for the activation of the C8 side chain. 3beta-hydroxycholest-5-en-26-oate can also be used as substrate.
History
EC 6.2.1.42 created 2014
Orthology
K18688  3-oxocholest-4-en-26-oate---CoA ligase
Genes
SPOI: IMCC21906_02605
ZAL: AZF00_12285
OSG: BST96_09090
HJA: BST95_13295
HALC: EY643_09180
CTES: O987_07490
HYN: F9K07_06005
DOE: DENOEST_1059(fadD) DENOEST_2572(fadD)
AZD: CDA09_09500
THU: AC731_008140
PLA: Plav_2206
RBM: TEF_02185
CAK: Caul_5298
PZU: PHZ_c2065
HNE: HNE_1963
BLAS: BSY18_3835
MTU: Rv3515c(fadD19)
MTC: MT3616
MRA: MRA_3554(fadD19)
MTUR: CFBS_3731(fadD19)
MTO: MTCTRI2_3579(fadD19)
MTN: ERDMAN_3854(fadD19)
MTUC: J113_24580
MTUE: J114_18795
MTUH: I917_24660
MTUL: TBHG_03455
MTUT: HKBT1_3716(fadD19)
MTUU: HKBT2_3725(fadD19)
MTQ: HKBS1_3728(fadD19)
MBO: BQ2027_MB3544C(fadD19)
MBB: BCG_3578c(fadD19)
MBT: JTY_3579(fadD19)
MBM: BCGMEX_3576c(fadD19)
MAF: MAF_35270(fadD19)
MMIC: RN08_3889
MCE: MCAN_35261(fadD19)
MCQ: BN44_110003(fadD)
MCV: BN43_90008(fadD)
MCX: BN42_90006(fadD)
MCZ: BN45_100005(fadD)
MPA: MAP_0550(fadD19) MAP_2380(fadD19) MAP_2388c(fadD19)
MLP: MLM_0787
MUL: MUL_3279 MUL_3870(fadD19_2) MUL_4075(fadD19_1)
MMI: MMAR_2064 MMAR_4007(fadD19_2) MMAR_5001(fadD19_1)
MMAE: MMARE11_20070 MMARE11_38140(fadD19_2) MMARE11_48110(fadD19_1)
MLI: MULP_02244 MULP_04172(fadD19_2) MULP_04178 MULP_05249(fadD19_1)
MHAD: B586_04730
MXE: MYXE_06320(fadD19) MYXE_17040
MVQ: MYVA_1893 MYVA_1909 MYVA_5099(fadD19_1)
MAB: MAB_4163c
MABB: MASS_4162
MCHE: BB28_21435
MSTE: MSTE_04338
ASD: AS9A_0991
MKR: MKOR_02430 MKOR_36020(fadD19)
NFA: NFA_5290
RER: RER_04580(fadD) RER_09360(fadD19) RER_33730 RER_51730(fadD) RER_53370
ROP: ROP_47740(fadD) ROP_47880(fadD19)
RRT: 4535765_00661(fadD19)
RCR: NCTC10994_02062(fadD19)
GOR: KTR9_0795 KTR9_3892(fadD19)
TPR: Tpau_2118
SMA: SAVERM_1246(fadD4)
SGR: SGR_6831
SGB: WQO_01045
SCB: SCAB_84891(fadD19)
SCT: SCAT_0755(fadD)
SBH: SBI_01089
SVE: SVEN_0280
SDV: BN159_8179(fadD19)
SFI: SFUL_326
SALU: DC74_1726
SALL: SAZ_09160
STRE: GZL_07420
SLD: T261_7158
STRM: M444_05970
SPRI: SPRI_6957
SNR: SNOUR_34120(fadD19)
SMAL: SMALA_0723
SLAU: SLA_0181
SALJ: SMD11_5900
SFK: KY5_7677c
SRJ: SRO_6654
CFL: Cfla_0290
NCA: Noca_2725
NDK: I601_3453
TFU: Tfu_2248
NAL: B005_3504
SRO: Sros_1485
MMAR: MODMU_2941
AOI: AORI_2536
AJA: AJAP_26145(fadD19) AJAP_28550
AMYY: YIM_14560
AORI: SD37_13735
PDX: Psed_2922
PSEA: WY02_25055
KAL: KALB_3405
ALO: CRK59571
SAQ: Sare_2836
CAI: Caci_2174
 » show all
Reference
1  [PMID:21602385]
  Authors
Wilbrink MH, Petrusma M, Dijkhuizen L, van der Geize R
  Title
FadD19 of Rhodococcus rhodochrous DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains.
  Journal
Appl Environ Microbiol 77:4455-64 (2011)
DOI:10.1128/AEM.00380-11
  Sequence
Reference
2  [PMID:24244004]
  Authors
Casabon I, Swain K, Crowe AM, Eltis LD, Mohn WW
  Title
Actinobacterial acyl coenzyme A synthetases involved in steroid side-chain catabolism.
  Journal
J Bacteriol 196:579-87 (2014)
DOI:10.1128/JB.01012-13
  Sequence
[rha:RHA1_ro04689] [mtu:Rv3515c]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.42
IUBMB Enzyme Nomenclature: 6.2.1.42
ExPASy - ENZYME nomenclature database: 6.2.1.42
BRENDA, the Enzyme Database: 6.2.1.42

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