KEGG   ENZYME: 6.2.1.42
Entry
EC 6.2.1.42                 Enzyme                                 
Name
3-oxocholest-4-en-26-oate---CoA ligase;
fadD19 (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
(25S)-3-oxocholest-4-en-26-oate:CoA ligase (AMP-forming)
Reaction(IUBMB)
ATP + (25S)-3-oxocholest-4-en-26-oate + CoA = AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA [RN:R10770]
Reaction(KEGG)
R10770
Substrate
ATP [CPD:C00002];
(25S)-3-oxocholest-4-en-26-oate [CPD:C21076];
CoA [CPD:C00010]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
(25S)-3-oxocholest-4-en-26-oyl-CoA [CPD:C20840]
Comment
The enzyme, characterized from actinobacterium Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol. It is responsible for the activation of the C8 side chain. 3beta-hydroxycholest-5-en-26-oate can also be used as substrate.
History
EC 6.2.1.42 created 2014
Orthology
K18688  3-oxocholest-4-en-26-oate---CoA ligase
Genes
SPOIIMCC21906_02605
SNANI6N98_12085
STAWNCG89_15940
ZALAZF00_12285
OSGBST96_09090
HJABST95_13295
HALCEY643_09180
AFUSEYZ66_05315
CTESO987_07490
HYNF9K07_06005
DOEDENOEST_1059(fadD) DENOEST_2572(fadD)
AZDCDA09_09500
THUAC731_008140
MSDMYSTI_04820
PLAPlav_2206
PMOBHG718_02530
RBSRHODOSMS8_02089
RBMTEF_02185
CAKCaul_5298
CMBCSW64_01995
CAUFCSW63_04890
CAULKCG34_06270
PZUPHZ_c2065
PHBHYN04_07985 HYN04_08445
HNEHNE_1963
NARSaro_1531 Saro_1563
SPHKSKP52_13335 SKP52_24420
SMAGAN936_14240 AN936_23785
STERAOA14_00610
SGISGRAN_0469 SGRAN_1179 SGRAN_1605(fadD19)
SPHOC3E99_01885 C3E99_02355
SPHXE5675_06100
SLANGV829_01790
SPAPH3Z74_23305
SPAIFPZ24_01035
SCLOSCLO_2002520
SPHGAZE99_15830
SFLASPHFLASMR4Y_00423(prpE) SPHFLASMR4Y_02314(prpE)
SPHYCHN51_00265 CHN51_05335
BLASBSY18_3835
PALGHFP57_10510
PARJJ4G78_10665
PHAOHF685_10655
SANDH3309_06030
POQKZX46_15590
PMEAKTC28_13775
ALHG6N82_09315
PMASNCF86_10465
TFVIDJ81_05095
CNAAB433_17920
EFVCHH26_02780
QCINCF85_12245
ELIELI_02000 ELI_14985
ELQGa0102493_111071
ERFFIU90_01740
PNSA9D12_05800
PORLBG023_11310
PHZCHX26_03665 CHX26_05405
POTE2E27_04720 E2E27_05245
MTURv3515c(fadD19)
MTVRVBD_3515c
MTCMT3616
MRAMRA_3554(fadD19)
MTFTBFG_13548
MTBTBMG_03554
MTKTBSG_03581
MTZTBXG_003530
MTECCDC5079_3255
MTURCFBS_3731(fadD19)
MTLCCDC5180_3208
MTOMTCTRI2_3579(fadD19)
MTNERDMAN_3854(fadD19)
MTJJ112_18925
MTUBMT7199_3575
MTUCJ113_24580
MTUEJ114_18795
MTXM943_18075
MTUHI917_24660
MTULTBHG_03455
MTUTHKBT1_3716(fadD19)
MTUUHKBT2_3725(fadD19)
MTQHKBS1_3728(fadD19)
MBOBQ2027_MB3544C(fadD19)
MBBBCG_3578c(fadD19)
MBTJTY_3579(fadD19)
MBMBCGMEX_3576c(fadD19)
MBKK60_036490
MAFMAF_35270(fadD19)
MMICRN08_3889
MCEMCAN_35261(fadD19)
MCQBN44_110003(fadD)
MCVBN43_90008(fadD)
MCXBN42_90006(fadD)
MCZBN45_100005(fadD)
MPAMAP_0550(fadD19) MAP_2380(fadD19) MAP_2388c(fadD19)
MAOMAP4_1435 MAP4_1443 MAP4_3317
MAVIRC58_07115 RC58_07155 RC58_16450
MAVURE97_07115 RE97_07155 RE97_16485
MAVMAV_0644 MAV_1592 MAV_1600
MITOCO_05530 OCO_16570 OCO_16690
MIAOCU_05580 OCU_16770 OCU_16890
MIDMIP_01013 MIP_02290 MIP_02304
MYOOEM_05610 OEM_14570 OEM_14690
MCHIAN480_02765 AN480_08155 AN480_08215
MIROCQ_05690 OCQ_14250 OCQ_14370
MMALCKJ54_02695 CKJ54_07015 CKJ54_07075
MLPMLM_0787
MMANMMAN_25030 MMAN_25160 MMAN_48490(fadD19)
MSAMycsm_02113 Mycsm_02128 Mycsm_05748
MULMUL_3279 MUL_3870(fadD19_2) MUL_4075(fadD19_1)
MMIMMAR_2064 MMAR_4007(fadD19_2) MMAR_5001(fadD19_1)
MMAEMMARE11_20070 MMARE11_38140(fadD19_2) MMARE11_48110(fadD19_1)
MLIMULP_02244 MULP_04172(fadD19_2) MULP_04178 MULP_05249(fadD19_1)
MPSEMPSD_20960 MPSD_39710 MPSD_51990(fadD19)
MSHOMSHO_04670(fadD19) MSHO_51040
MMCMmcs_1775 Mmcs_1786 Mmcs_2891 Mmcs_4627
MKMMkms_1822 Mkms_1833 Mkms_2935 Mkms_4715
MJLMjls_1756 Mjls_1767 Mjls_2921 Mjls_5009
MMMW7S_02715 W7S_06955 W7S_07015
MKNMKAN_06070 MKAN_09620 MKAN_11735 MKAN_22955 MKAN_25740
MYVG155_04840 G155_10770 G155_10840 G155_15435 G155_16740 G155_23265
MYEAB431_00500 AB431_25020 AB431_26250
MGOAFA91_11180 AFA91_21470
MHADB586_04730
MDXBTO20_15255 BTO20_24115 BTO20_24170 BTO20_31085
MSHGMSG_01920 MSG_03595 MSG_03606 MSG_04501(fadD19)
MFJMFLOJ_07140 MFLOJ_13380 MFLOJ_41220(fadD19) MFLOJ_50790 MFLOJ_50890
MSTOMSTO_08490 MSTO_14790 MSTO_45210(fadD19) MSTO_54980
MSIMMSIM_04310 MSIM_29610(fadD19) MSIM_40500
MSAKMSAS_00180 MSAS_18570 MSAS_28580(fadD19) MSAS_57080
MKUI2456_10195 I2456_19045 I2456_25395
MGROFZ046_02510 FZ046_19675
MXEMYXE_06320(fadD19) MYXE_17040
MNVMNVI_02850 MNVI_02930 MNVI_11970(fadD19)
MPAGC0J29_11885 C0J29_22035 C0J29_22085 C0J29_27440
MNMMNVM_24320 MNVM_26850 MNVM_27150 MNVM_36400
MGORH0P51_07365 H0P51_20010 H0P51_20065 H0P51_21800 H0P51_25490 H0P51_27805 H0P51_27835
MCOOMCOO_16490 MCOO_16660 MCOO_26520(fadD19)
MBAIMB901379_01889 MB901379_03486 MB901379_03494 MB901379_04394
MSEOMSEO_07150 MSEO_23860 MSEO_23970 MSEO_33970(fadD19)
MHEKJMUB5695_00531(fadD19) JMUB5695_01773
MGAUMGALJ_04390 MGALJ_04520 MGALJ_37760
MLJMLAC_00270(fadD19)
MBRDMBRA_02250 MBRA_20570(fadD19) MBRA_31650 MBRA_31790 MBRA_58070 MBRA_58170
MSHJMSHI_39250(fadD19)
MKRMKOR_02430 MKOR_36020(fadD19)
MDFK0O62_25310
MMAMK3U93_17130 K3U93_17170 K3U93_21425 K3U93_22370
MHOLK3U96_03425 K3U96_17800 K3U96_17885
MHERK3U94_13635 K3U94_19160 K3U94_19285 K3U94_20630
MSENK3U95_07545 K3U95_24985
MPAEK0O64_00480 K0O64_24715 K0O64_25740
MSPGF6B93_20195
MOTLTS72_00095 LTS72_14290 LTS72_14450 LTS72_17135
MVMMJO54_13425 MJO54_19120 MJO54_19240 MJO54_20560
MRFMJO55_23795
MPAAMKK62_02700 MKK62_07655 MKK62_07720
MCROMI149_01005 MI149_26050
MSMMSMEG_5914
MSGMSMEI_2177(fadD19) MSMEI_2188 MSMEI_3600 MSMEI_5754(fadD19)
MSBLJ00_11105 LJ00_11160 LJ00_18330 LJ00_29245
MSNLI99_11105 LI99_11160 LI99_18335 LI99_29250
MSHLI98_11110 LI98_11165 LI98_18340 LI98_29255
MVAMvan_1995 Mvan_2010 Mvan_5215
MGIMflv_1543 Mflv_4335 Mflv_4349
MSPMspyr1_09270
MCBMycch_1739 Mycch_1752 Mycch_4576
MNED174_03230 D174_16020 D174_24120
MYNMyAD_03155 MyAD_15740 MyAD_23660
MFTXA26_09300 XA26_22160 XA26_22300 XA26_34290
MPHLMPHLCCUG_00593 MPHLCCUG_01907 MPHLCCUG_01924
MVQMYVA_1893 MYVA_1909 MYVA_5099(fadD19_1)
MLLB1R94_00535 B1R94_24780
MRHMycrhN_2319 MycrhN_6046 MycrhN_6062
MTHN4412656_01433 4412656_01448 4412656_02213 4412656_04032
MHASMHAS_00825 MHAS_00842 MHAS_03700
MDUMDUV_24900 MDUV_25050 MDUV_49930
MCHTMCHIJ_09990 MCHIJ_20160 MCHIJ_21060 MCHIJ_21200
MAUUNCTC10437_01838 NCTC10437_01867 NCTC10437_04977
MMAGMMAD_30880 MMAD_47750
MMORMMOR_01320 MMOR_01410 MMOR_12500 MMOR_44850 MMOR_44980
MFXMFAL_25980 MFAL_33790 MFAL_34760
MAICMAIC_05980 MAIC_07610 MAIC_55610
MIJMINS_07000 MINS_42160 MINS_43030
MALVMALV_05730 MALV_16820 MALV_16960 MALV_43050
MTYMTOK_12820 MTOK_23130
MPSCMPSYJ_16510 MPSYJ_46350
MARZMARA_10370 MARA_48680
MGADMGAD_19580 MGAD_19730 MGAD_36840
MHEVMHEL_21070 MHEL_28440(fadD19) MHEL_29920 MHEL_39580
MSARMSAR_10340 MSAR_16920
MANYMANY_26380 MANY_32380 MANY_37310
MAUBMAUB_12020 MAUB_13890 MAUB_26050
MPOFMPOR_23270 MPOR_23450 MPOR_53160
MPHUMPHO_00790 MPHO_44290
MMUCC1S78_017980 C1S78_025140
MMATMMAGJ_35090
MBOKMBOE_03690
MFGK6L26_05845
MSEIMSEDJ_01730
MFLVNCTC10271_00537 NCTC10271_03527 NCTC10271_03592
MCEEMCEL_17730 MCEL_38530
MMONEWR22_25025
MABMAB_4163c
MMVMYCMA_2317
MABBMASS_4162
MABLMMASJCM_4248
MCHEBB28_21435
MIZBAB75_22885 BAB75_23830
MSTEMSTE_04338
MSAOMYCSP_19470
MSALDSM43276_04003
MJDJDM601_2408 JDM601_3360(fadD19_2) JDM601_3390 JDM601_3648(fadD19_1)
MTER4434518_02338 4434518_03346(fadD19_2) 4434518_03376 4434518_03622(fadD19_1)
MMINMMIN_01140 MMIN_10540 MMIN_11770
MHIBMHIB_18610 MHIB_27810 MHIB_28060 MHIB_30460
ASDAS9A_0991
MPAKMIU77_02300 MIU77_05470 MIU77_10410
NFANFA_5290
NFRERS450000_03702
NCYNOCYR_0542
NBRO3I_002930 O3I_020185
NNONONO_c03470 NONO_c05390 NONO_c32970 NONO_c33060 NONO_c38820 NONO_c43920
NSLBOX37_02385 BOX37_22105
NSRNS506_00416 NS506_01306 NS506_01309 NS506_02683 NS506_02684
NTPCRH09_03510
NOZDMB37_00305 DMB37_23825
NODFOH10_28465 FOH10_29795
NAHF5544_02860
NADNCTC11293_01290 NCTC11293_04157
NWLNWFMUON74_05350(fadD19) NWFMUON74_28880
NIEKV110_03245 KV110_20615
NTCKHQ06_03420 KHQ06_07775
NHUH0264_02980 H0264_04070 H0264_04085
NYALTV02_28550 LTV02_28670 LTV02_29765
NGPLTT66_00995 LTT66_15740
RHARHA1_ro04675 RHA1_ro04689(fadD19) RHA1_ro10299
RERRER_04580(fadD) RER_09360(fadD19) RER_33730 RER_51730(fadD) RER_53370
REYO5Y_02195 O5Y_04175 O5Y_15540 O5Y_24465 O5Y_25335
REBXU06_02400 XU06_04420 XU06_15775 XU06_24885 XU06_25685
RQIC1M55_02495 C1M55_04655 C1M55_16790 C1M55_26085 C1M55_26885
ROPROP_47740(fadD) ROP_47880(fadD19)
ROAPd630_LPD01181 Pd630_LPD01195
RPYY013_19435
RBYCEJ39_05795
RAVAAT18_04190 AAT18_22230
RHWBFN03_14680
RRZCS378_12020 CS378_19820
RHQIM25_09185
RHODAOT96_05895 AOT96_06880 AOT96_12950 AOT96_15060 AOT96_26975
RRT4535765_00661(fadD19)
RCRNCTC10994_02062(fadD19)
RTMG4H71_06990
RKOJWS14_25900 JWS14_25970
RGOKYT97_05630 KYT97_15720 KYT97_21905 KYT97_24380
ROZCBI38_04940 CBI38_05005
RPSKJWS13_18530 JWS13_18600
RGORNMQ04_02915 NMQ04_08035
REQREQ_07350 REQ_13860 REQ_36170 REQ_43880
GBRGbro_0888 Gbro_3959
GPOGPOL_c25330 GPOL_c41480 GPOL_c42730
GORKTR9_0795 KTR9_3892(fadD19)
GOQACH46_03155 ACH46_17425
GTABCM27_04360 BCM27_20410
GOCCXX93_06325
GITC6V83_04180
GRUGCWB2_04070 GCWB2_19895
GOMD7316_02594 D7316_04524
GAVC5O27_00970 C5O27_08225
GODGKZ92_03820 GKZ92_18815
GAMGII34_06215 GII34_19560 GII34_19850 GII34_20150 GII34_20230
GPDGII33_02620 GII33_02645 GII33_02945 GII33_03300
GJIH1R19_03770 H1R19_18755
GOILK459_05245 LK459_13855
GHNMVF96_04135 MVF96_19330
GAMIIHQ52_06710 IHQ52_22830
GMGNWF22_06295 NWF22_15315
SPINKV203_17145
TPRTpau_2118
TSMASU32_11520
TPULTPB0596_24700
DTMBJL86_0674 BJL86_0678 BJL86_2458 BJL86_3087
DITC3V38_09180
DIZCT688_03095 CT688_12035
DPCA6048_03040 A6048_03395 A6048_12575
DLUA6035_03340 A6035_03645 A6035_11660
DKNNHB83_02890 NHB83_03225 NHB83_12025
TOYFO059_01365 FO059_05020 FO059_14030
SMASAVERM_1246(fadD4)
SGRSGR_6831
SGBWQO_01045
SCBSCAB_84891(fadD19)
SVLStrvi_5755
SRCM271_43355
SMALSMALA_0723
SSOII1A49_07210
SBHSBI_01089
SVESVEN_0280
SDVBN159_8179(fadD19)
SFISFUL_326
SCIB446_04725
SVTSVTN_02935
STREGZL_07420
SLDT261_7158
SLCSL103_13405
STRMM444_05970
SPRISPRI_6957
SCZABE83_34015
SCXAS200_40345
SRWTUE45_00992
SCLFBB341_26935
SGSAVL59_27795
STSIA4E84_02705
SALWCP975_32720
SGMGCM10017557_76110
SCADDN051_34325
SPHWNFX46_24145
SLSSLINC_0577 SLINC_7434
SNRSNOUR_34120(fadD19)
SALUDC74_1726
SALLSAZ_09160
SPLULK06_030440
SAUOBV401_07465
SSIAA7J05_03655
SVUB1H20_00635
SPUNBFF78_38690
SGVB1H19_07980
SLAUSLA_0181
SALFSMD44_00716
SALJSMD11_5900
SLXSLAV_32970
STROSTRMOE7_06595
SFKKY5_7677c
SNZDC008_02735
SGEDWG14_07807
SRJSRO_6654
SLKSLUN_03045
SDXC4B68_37045
SGDELQ87_36515
SQZFQU76_00870
SCYAEJ357_44110
STIRDDW44_29665
SKACP970_01125
SGZC0216_18560
SVNCP980_29910
SHAWCEB94_03200
SRKFGW37_03535
SFICEIZ62_31135
SGALCP966_01335
SFYGFH48_37065
SAQUEJC51_06310
SGFHEP81_06997
SCAVCVT27_00500
SRIMCP984_35315
SSUBCP968_29940
SPHVF9278_02275
STSUB7R87_32650
SCHACP983_40470
SGXH4W23_03730
SCOECP976_01060
SSPBCP982_39155
SNFJYK04_01256
SPLACP981_05200
SBROGQF42_06215
STUIGCM10017668_65560
SATAC5746_02995
SHUNDWB77_06660
SCYGS1361_03550
SFEUIM697_26065 IM697_28310
SDWK7C20_04585
SVDCP969_01180
SCALI6J39_00760
SAUHSU9_003860
SPEUCGZ69_02445
SROIIAG44_02855
SXNIAG42_32530
SHKJ2N69_34790
SANUK7396_30885
SGOBtest1122_25420
SINEKI385_07670
SLFJEQ17_44295
SDECL3078_02705
SAOVG3H79_34970
SDURM4V62_38025
SYANNRK68_03425
SAKBK1J60_39165 K1J60_42665
STAALDH80_05365
SCTSCAT_0755(fadD)
SCYSCATT_07570
KABB7C62_00540
YIALO772_25930 LO772_27755
STRHGXP74_24155
MLVCVS47_02988
MWAE4K62_16790 E4K62_17580
MPRTET475_01820
MCAWF6J84_13340 F6J84_14295
MLZF6J85_16665
MBINLXM64_14095 LXM64_15015
CFLCfla_0290
NCANoca_2725
NDKI601_3453
NBEBack2_27850
NANOG5V58_10050
NAROCFH99_06225 CFH99_13295
NPIG7071_00410
NPCKUV85_04460 KUV85_17150
PSIMKR76_14210 KR76_21465
AEBC6I20_03475
TFUTfu_2248
THAONI17_014010
TALXFOF52_11320
NALB005_3504
NGVCDO52_23705
NEXNE857_10065
STRREKD16_07035
SNAHOUQ99_24150
TCUTcur_0691 Tcur_3496 Tcur_3980 Tcur_4748
ACTWF7P10_13525 F7P10_22090 F7P10_26225
AGRAAGRA3207_002837 AGRA3207_004371
SROSros_1485
NOABKM31_38170
NOWGBF35_07150
NCXNocox_06585
NGNLCN96_07205
FRAFrancci3_1806
FRIFraEuI1c_1369 FraEuI1c_1935
MMARMODMU_2941
SACGFDZ84_22820
AMDAMED_2559 AMED_3881
AMNRAM_13005 RAM_19765
AMMAMES_2531 AMES_3836
AMZB737_2532 B737_3836
AOIAORI_2536
AJAAJAP_26145(fadD19) AJAP_28550
AMQAMETH_3173 AMETH_3762 AMETH_4814
AMYCCU254_10595
AMYBBKN51_05290
AABA4R43_15885
AMYYYIM_14560
AORISD37_13735
AACDLWP59_22695 LWP59_28995
APRTMUY14_23615
AROONQK81_09665
ATHML1857_26815
PDXPsed_2922
PSEAWY02_25055
PAUTPdca_48200 Pdca_65400
PPELH6H00_29265
KALKALB_3405
KPHYAOZ06_42485
LEDBBK82_38740
ALOCRK59571
PMADBAY61_11030
SACEGIY23_13265
STPStrop_2639
SAQSare_2836
AMSAMIS_19450
AIHAiant_18570
PFLAPflav_030620
PSUUPsuf_082940
CAICaci_2174
ABAIIMCC26256_111261 IMCC26256_111379 IMCC26256_111846 IMCC26256_112054
AYMYM304_15600 YM304_31200 YM304_35190
ATQGH723_02270 GH723_02375 GH723_02405 GH723_06495 GH723_09705 GH723_10650
AQZKSP35_00645 KSP35_01185 KSP35_04590 KSP35_07195 KSP35_07205 KSP35_09110 KSP35_09215 KSP35_13515
IAMHC251_05225 HC251_13675 HC251_15155 HC251_16360 HC251_17885 HC251_17955 HC251_19690 HC251_19840
 » show all
Reference
1  [PMID:21602385]
  Authors
Wilbrink MH, Petrusma M, Dijkhuizen L, van der Geize R
  Title
FadD19 of Rhodococcus rhodochrous DSM43269, a steroid-coenzyme A ligase essential for degradation of C-24 branched sterol side chains.
  Journal
Appl Environ Microbiol 77:4455-64 (2011)
DOI:10.1128/AEM.00380-11
  Sequence
Reference
2  [PMID:24244004]
  Authors
Casabon I, Swain K, Crowe AM, Eltis LD, Mohn WW
  Title
Actinobacterial acyl coenzyme A synthetases involved in steroid side-chain catabolism.
  Journal
J Bacteriol 196:579-87 (2014)
DOI:10.1128/JB.01012-13
  Sequence
[rha:RHA1_ro04689] [mtu:Rv3515c]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.42
IUBMB Enzyme Nomenclature: 6.2.1.42
ExPASy - ENZYME nomenclature database: 6.2.1.42
BRENDA, the Enzyme Database: 6.2.1.42

DBGET integrated database retrieval system