EC                 Enzyme                                 

long-chain fatty acid adenylyltransferase FadD28;
fadD28 (gene name)
Forming carbon-sulfur bonds;
Acid-thiol ligases
long-chain fatty acid:holo-[mycocerosate synthase] ligase (AMP-forming)
ATP + a long-chain fatty acid + holo-[mycocerosate synthase] = AMP + diphosphate + a long-chain acyl-[mycocerosate synthase] (overall reaction);
(1a) ATP + a long-chain fatty acid = diphosphate + a long-chain acyl-adenylate ester;
(1b) a long-chain acyl-adenylate ester + holo-[mycocerosate synthase] = AMP + a long-chain acyl-[mycocerosate synthase]
ATP [CPD:C00002];
long-chain fatty acid [CPD:C00638];
holo-[mycocerosate synthase];
long-chain acyl-adenylate ester
AMP [CPD:C00020];
diphosphate [CPD:C00013];
long-chain acyl-[mycocerosate synthase];
long-chain acyl-adenylate ester
The enzyme, found in certain mycobacteria, activates long-chain fatty acids by adenylation and transfers them to EC, mycocerosate synthase. The enzyme participates in the biosynthesis of the virulent lipids dimycocerosates (DIM) and dimycocerosyl triglycosyl phenolphthiocerol (PGL).
EC created 2016 as EC, transferred 2017 to EC
K12427  long-chain fatty acid adenylyltransferase FadD28
MTU: Rv2941(fadD28)
MTV: RVBD_1521 RVBD_1529 RVBD_2941
MTC: MT3011
MRA: MRA_1533(fadD25) MRA_1541(fadD24) MRA_2967(fadD28)
MTF: TBFG_11554 TBFG_11562 TBFG_12955
MTB: TBMG_01031 TBMG_02448 TBMG_02456
MTK: TBSG_01039 TBSG_02460 TBSG_02468
MTZ: TBXG_001019 TBXG_002429 TBXG_002437
MTG: MRGA327_09540 MRGA327_09595 MRGA327_18075
MTI: MRGA423_09540 MRGA423_09585
MTUR: CFBS_1622(fadD25) CFBS_1630(fadD24) CFBS_3099(fadD28)
MTO: MTCTRI2_1564(fadD25) MTCTRI2_1572(fadD24) MTCTRI2_2998(fadD28)
MTD: UDA_1521(fadD25) UDA_1529(fadD24) UDA_2941(fadD28)
MTN: ERDMAN_1696(fadD25) ERDMAN_1705(fadD24) ERDMAN_3223(fadD28)
MTUT: HKBT1_1622(fadD25) HKBT1_1630(fadD24) HKBT1_3087(fadD28)
MTUU: HKBT2_1629(fadD25) HKBT2_1637(fadD24) HKBT2_3092(fadD28)
MTQ: HKBS1_1626(fadD25) HKBS1_1634(fadD24) HKBS1_3094(fadD28)
MBO: BQ2027_MB1548(fadD25) BQ2027_MB1556(fadD24) BQ2027_MB2966(fadD28)
MBB: BCG_2963(fadD28)
MBT: JTY_1548(fadD25) JTY_1556(fadD24) JTY_2958(fadD28)
MBM: BCGMEX_1545(fadD25) BCGMEX_1553(fadD24) BCGMEX_2958(fadD28)
MAF: MAF_15480(fadD25) MAF_15560(fadD24) MAF_29460(fadD28)
MCE: MCAN_15421(fadD25) MCAN_15521(fadD24) MCAN_29631(fadD28)
MCQ: BN44_20085(fadD) BN44_20093(fadD) BN44_60418(fadD)
MCV: BN43_30642(fadD) BN43_30655(fadD) BN43_40650(fadD)
MCX: BN42_21451(fadD) BN42_21460(fadD) BN42_40940(fadD)
MCZ: BN45_30628(fadD) BN45_40004(fadD) BN45_51353(fadD)
MLE: ML0138(fadD28)
MLB: MLBr00138(fadD28)
MPA: MAP_3752(fadD28)
MAO: MAP4_0017
MAVI: RC58_00085
MAVU: RE97_00085
MIT: OCO_23760
MIA: OCU_23640
MID: MIP_03280
MYO: OEM_22180
MIR: OCQ_22290
MMAN: MMAN_15980(fadD28)
MUL: MUL_1432(fadD28_1) MUL_2008(fadD28)
MMI: MMAR_1765(fadD28) MMAR_2341(fadD25)
MMAE: MMARE11_16840(fadD28) MMARE11_22630(fadD25)
MMM: W7S_11460
MLI: MULP_01920(fadD28)
MSHG: MSG_02209(fadD28)
MSAK: MSAS_03050 MSAS_29300(fadD28)
MXE: MYXE_02300(fadD28)
MNV: MNVI_16080(fadD28)
MCOO: MCOO_24850(fadD28)
MPSE: MPSD_13330 MPSD_40710(fadD28)
MSHO: MSHO_54770(fadD28)
MGAU: MGALJ_24050 MGALJ_58710(fadD28)
MLJ: MLAC_13800(fadD25) MLAC_14030(fadD24) MLAC_28790(fadD28)
MBRD: MBRA_32650(fadD28)
MSHJ: MSHI_07260(fadD28) MSHI_16700(fadD24)
MHAS: MHAS_03481
MCHT: MCHIJ_19900(fadD24)
MAUU: NCTC10437_02878(fadD28)
MMAG: MMAD_24270(fadD28)
MAIC: MAIC_39590(fadD28)
MALV: MALV_42820(fadD28)
MTY: MTOK_49720(fadD28)
MGAD: MGAD_24310(fadD28)
MHEV: MHEL_06730(fadD28)
MSAR: MSAR_29630(fadD28)
MANY: MANY_23040(fadD28)
MBOK: MBOE_03930(fadD28_1)
 » show all
1  [PMID:9098059]
Fitzmaurice AM, Kolattukudy PE
Open reading frame 3, which is adjacent to the mycocerosic acid synthase gene, is expressed as an acyl coenzyme A synthase in Mycobacterium bovis BCG.
J Bacteriol 179:2608-15 (1997)
2  [PMID:16582482]
Goyal A, Yousuf M, Rajakumara E, Arora P, Gokhale RS, Sankaranarayanan R
Crystallization and preliminary X-ray crystallographic studies of the N-terminal  domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis.
Acta Crystallogr Sect F Struct Biol Cryst Commun 62:350-2 (2006)
3  [PMID:19182784]
Arora P, Goyal A, Natarajan VT, Rajakumara E, Verma P, Gupta R, Yousuf M, Trivedi OA, Mohanty D, Tyagi A, Sankaranarayanan R, Gokhale RS
Mechanistic and functional insights into fatty acid activation in Mycobacterium tuberculosis.
Nat Chem Biol 5:166-73 (2009)
4  [PMID:24831705]
Menendez-Bravo S, Comba S, Sabatini M, Arabolaza A, Gramajo H
Expanding the chemical diversity of natural esters by engineering a polyketide-derived pathway into Escherichia coli.
Metab Eng 24:97-106 (2014)
5  [PMID:25561717]
Vergnolle O, Chavadi SS, Edupuganti UR, Mohandas P, Chan C, Zeng J, Kopylov M, Angelo NG, Warren JD, Soll CE, Quadri LE
Biosynthesis of cell envelope-associated phenolic glycolipids in Mycobacterium marinum.
J Bacteriol 197:1040-50 (2015)
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