KEGG   ENZYME: 6.2.1.52Help
Entry
EC 6.2.1.52                 Enzyme                                 

Name
L-firefly luciferin---CoA ligase;
LUC
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
(R)-4,5-dihydro-2-(6-hydroxy-1,3-benzothiazol-2-yl)thiazole-4-carboxylate:CoA ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-firefly luciferin + CoA = AMP + diphosphate + L-firefly luciferyl-CoA [RN:R11926]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
L-firefly luciferin [CPD:C21771];
CoA [CPD:C00010]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-firefly luciferyl-CoA [CPD:C21772]
Comment
This is an alternative activity of the firefly luciferase (EC 1.13.12.7), which the enzyme exhibits under normal conditions only when acting on the L-enantiomer of its substrate. The D-isomer can act as a substrate for the CoA---ligase activity in vitro only under low oxygen conditions that are not found in vivo. The activation of L-firefly luciferin to a CoA ester is a step in a recycling pathway that results in its epimerization to the D enantiomer, which is the only substrate whose oxygenation results in light emission.
History
EC 6.2.1.52 created 2017
Reference
1  [PMID:14695520]
  Authors
Fraga H, Esteves da Silva JC, Fontes R
  Title
Identification of luciferyl adenylate and luciferyl coenzyme a synthesized by firefly luciferase.
  Journal
Chembiochem 5:110-5 (2004)
DOI:10.1002/cbic.200300735
Reference
2  [PMID:15850783]
  Authors
Nakamura M, Maki S, Amano Y, Ohkita Y, Niwa K, Hirano T, Ohmiya Y, Niwa H
  Title
Firefly luciferase exhibits bimodal action depending on the luciferin chirality.
  Journal
Biochem Biophys Res Commun 331:471-5 (2005)
DOI:10.1016/j.bbrc.2005.03.202
Reference
3  [PMID:20526507]
  Authors
Viviani VR, Scorsato V, Prado RA, Pereira JG, Niwa K, Ohmiya Y, Barbosa JA
  Title
The origin of luciferase activity in Zophobas mealworm AMP/CoA-ligase (protoluciferase): luciferin stereoselectivity as a switch for the oxygenase activity.
  Journal
Photochem Photobiol Sci 9:1111-9 (2010)
DOI:10.1039/c0pp00080a
Reference
4  [PMID:28454735]
  Authors
Maeda J, Kato DI, Okuda M, Takeo M, Negoro S, Arima K, Ito Y, Niwa K
  Title
Biosynthesis-inspired deracemizative production of d-luciferin by combining luciferase and thioesterase.
  Journal
Biochim Biophys Acta 1861:2112-2118 (2017)
DOI:10.1016/j.bbagen.2017.04.010
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.52
IUBMB Enzyme Nomenclature: 6.2.1.52
ExPASy - ENZYME nomenclature database: 6.2.1.52
BRENDA, the Enzyme Database: 6.2.1.52

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