KEGG   ENZYME: 6.2.1.53
Entry
EC 6.2.1.53                 Enzyme                                 

Name
L-proline---[L-prolyl-carrier protein] ligase;
pltF (gene name);
bmp4 (gene name);
pigI (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
L-proline:[L-prolyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-proline + holo-[L-prolyl-carrier protein] = AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] (overall reaction);
(1a) ATP + L-proline = diphosphate + (L-prolyl)adenylate [RN:R12056];
(1b) (L-prolyl)adenylate + holo-[L-prolyl-carrier protein] = AMP + L-prolyl-[L-prolyl-carrier protein]
Reaction(KEGG)
R12056;
(other) R06746 R12057
Substrate
ATP [CPD:C00002];
L-proline [CPD:C00148];
holo-[L-prolyl-carrier protein];
(L-prolyl)adenylate [CPD:C20656]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-prolyl-[L-prolyl-carrier protein];
(L-prolyl)adenylate [CPD:C20656]
Comment
The enzyme participates in the biosynthesis of several pyrrole-containing compounds, such as undecylprodigiosin, prodigiosin, pyoluteorin, and coumermycin A1. It catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein.
History
EC 6.2.1.53 created 2018
Pathway
ec00333  Prodigiosin biosynthesis
ec00401  Novobiocin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12719  L-proline---[L-prolyl-carrier protein] ligase
K21780  L-proline---[L-prolyl-carrier protein] ligase
Genes
SMW: SMWW4_v1c10960(pigI)
SRR: SerAS9_1745
SRS: SerAS12_1745
SRA: SerAS13_1746
SERF: L085_23070
SERA: Ser39006_020655
SERQ: CWC46_20660
SERM: CLM71_22570
SSUR: ATE40_002195
VGA: BSQ33_00995
HCH: HCH_06032
SCO: SCO5891(redM)
STRD: NI25_09775
 » show all
Reference
1  [PMID:11880032]
  Authors
Thomas MG, Burkart MD, Walsh CT.
  Title
Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis.
  Journal
Chem Biol 9:171-84 (2002)
DOI:10.1016/S1074-5521(02)00100-X
Reference
2  [PMID:15528645]
  Authors
Harris AK, Williamson NR, Slater H, Cox A, Abbasi S, Foulds I, Simonsen HT, Leeper FJ, Salmond GP
  Title
The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation.
  Journal
Microbiology 150:3547-60 (2004)
DOI:10.1099/mic.0.27222-0
  Sequence
Reference
3  [PMID:15853884]
  Authors
Williamson NR, Simonsen HT, Ahmed RA, Goldet G, Slater H, Woodley L, Leeper FJ, Salmond GP
  Title
Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces.
  Journal
Mol Microbiol 56:971-89 (2005)
DOI:10.1111/j.1365-2958.2005.04602.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.53
IUBMB Enzyme Nomenclature: 6.2.1.53
ExPASy - ENZYME nomenclature database: 6.2.1.53
BRENDA, the Enzyme Database: 6.2.1.53

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