KEGG   ENZYME: 6.2.1.53
Entry
EC 6.2.1.53                 Enzyme                                 
Name
L-proline---[L-prolyl-carrier protein] ligase;
pltF (gene name);
bmp4 (gene name);
pigI (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
L-proline:[L-prolyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-proline + holo-[L-prolyl-carrier protein] = AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] (overall reaction);
(1a) ATP + L-proline = diphosphate + (L-prolyl)adenylate [RN:R12056];
(1b) (L-prolyl)adenylate + holo-[L-prolyl-carrier protein] = AMP + L-prolyl-[L-prolyl-carrier protein]
Reaction(KEGG)
R12056;
(other) R06746 R12057
Substrate
ATP [CPD:C00002];
L-proline [CPD:C00148];
holo-[L-prolyl-carrier protein];
(L-prolyl)adenylate [CPD:C20656]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-prolyl-[L-prolyl-carrier protein];
(L-prolyl)adenylate [CPD:C20656]
Comment
The enzyme participates in the biosynthesis of several pyrrole-containing compounds, such as undecylprodigiosin, prodigiosin, pyoluteorin, and coumermycin A1. It catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein.
History
EC 6.2.1.53 created 2018
Pathway
ec00333  Prodigiosin biosynthesis
ec00401  Novobiocin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12719  L-proline---[L-prolyl-carrier protein] ligase
K21780  L-proline---[L-prolyl-carrier protein] ligase
Genes
SMWSMWW4_v1c10960(pigI)
SRRSerAS9_1745
SRSSerAS12_1745
SRASerAS13_1746
SERFL085_23070
SERASer39006_020655
SERQCWC46_20660
SERMCLM71_22570
SSURATE40_002195
SNEMNLX84_05465
VGABSQ33_00995
VSRVspart_01686(pigI)
PRRAT705_06620
PTUPTUN_a2940
PVBJ5X90_18250
PSAZPA25_35560
LANTTUM19329_13350
HCHHCH_06032
HAHEENC22_25985
HPROLMS44_05405
CCUPBKK81_22625
CARIFNU76_06130
KIAG8A07_12235
MDRMDOR_11230
NTPCRH09_26890
NAHF5544_12760
SCOSCO5891(redM)
SLVSLIV_09150
SGMGCM10017557_78140 GCM10017557_82750
STRDNI25_09775
SSIAA7J05_21360
SALFSMD44_07462
SRKFGW37_26810
SPADDVK44_30970
SRIMCP984_39845
SSPBCP982_34450
SFEUIM697_30930
SLFJEQ17_02545
SAOVG3H79_04215
ARUBJ5A65_13345
NGVCDO52_00495
PSUUPsuf_018720
CATICS0771_15580
 » show all
Reference
1  [PMID:11880032]
  Authors
Thomas MG, Burkart MD, Walsh CT.
  Title
Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis.
  Journal
Chem Biol 9:171-84 (2002)
DOI:10.1016/S1074-5521(02)00100-X
Reference
2  [PMID:15528645]
  Authors
Harris AK, Williamson NR, Slater H, Cox A, Abbasi S, Foulds I, Simonsen HT, Leeper FJ, Salmond GP
  Title
The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation.
  Journal
Microbiology 150:3547-60 (2004)
DOI:10.1099/mic.0.27222-0
  Sequence
Reference
3  [PMID:15853884]
  Authors
Williamson NR, Simonsen HT, Ahmed RA, Goldet G, Slater H, Woodley L, Leeper FJ, Salmond GP
  Title
Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces.
  Journal
Mol Microbiol 56:971-89 (2005)
DOI:10.1111/j.1365-2958.2005.04602.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.53
IUBMB Enzyme Nomenclature: 6.2.1.53
ExPASy - ENZYME nomenclature database: 6.2.1.53
BRENDA, the Enzyme Database: 6.2.1.53

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