KEGG   ENZYME: 6.2.1.54
Entry
EC 6.2.1.54                 Enzyme                                 

Name
D-alanine---[D-alanyl-carrier protein] ligase;
dltA (gene name);
Dcl
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
D-alanine:[D-alanyl-carrier protein] ligase
Reaction(IUBMB)
ATP + D-alanine + holo-[D-alanyl-carrier protein] = AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein] (overall reaction);
(1a) ATP + D-alanine = (D-alanyl)adenylate + diphosphate;
(1b) (D-alanyl)adenylate + holo-[D-alanyl-carrier protein] = AMP + D-alanyl-[D-alanyl-carrier protein]
Substrate
ATP [CPD:C00002];
D-alanine [CPD:C00133];
holo-[D-alanyl-carrier protein];
(D-alanyl)adenylate
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
D-alanyl-[D-alanyl-carrier protein];
(D-alanyl)adenylate
Comment
The enzyme is involved in the modification of wall teichoic acids, as well as type I and IV lipoteichoic acids, with D-alanine residues. It activates D-alanine using ATP to form a high-energy (D-alanyl)adenylate intermediate and subsequently transfers the alanyl moiety to the phosphopantheinyl prosthetic group of a D-alanyl-carrier protein (DltC).
History
EC 6.2.1.54 created 2018
Reference
1  [PMID:7797557]
  Authors
Perego M, Glaser P, Minutello A, Strauch MA, Leopold K, Fischer W
  Title
Incorporation of D-alanine into lipoteichoic acid and wall teichoic acid in Bacillus subtilis. Identification of genes and regulation.
  Journal
J Biol Chem 270:15598-606 (1995)
DOI:10.1074/jbc.270.26.15598
  Sequence
[bsu:BSU38500]
Reference
2  [PMID:18784082]
  Authors
Yonus H, Neumann P, Zimmermann S, May JJ, Marahiel MA, Stubbs MT
  Title
Crystal structure of DltA. Implications for the reaction mechanism of non-ribosomal peptide synthetase adenylation domains.
  Journal
J Biol Chem 283:32484-91 (2008)
DOI:10.1074/jbc.M800557200
  Sequence
[bsu:BSU38500]
Reference
3  [PMID:18847223]
  Authors
Du L, He Y, Luo Y
  Title
Crystal structure and enantiomer selection by D-alanyl carrier protein ligase DltA from Bacillus cereus.
  Journal
Biochemistry 47:11473-80 (2008)
DOI:10.1021/bi801363b
  Sequence
[bce:BC1372]
Reference
4  [PMID:19324056]
  Authors
Osman KT, Du L, He Y, Luo Y
  Title
Crystal structure of Bacillus cereus D-alanyl carrier protein ligase (DltA) in complex with ATP.
  Journal
J Mol Biol 388:345-55 (2009)
DOI:10.1016/j.jmb.2009.03.040
  Sequence
[bce:BC1372]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.54
IUBMB Enzyme Nomenclature: 6.2.1.54
ExPASy - ENZYME nomenclature database: 6.2.1.54
BRENDA, the Enzyme Database: 6.2.1.54

DBGET integrated database retrieval system