Entry
Name
3,4-dihydroxybenzoate---[aryl-carrier protein] ligase;
asbC (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
3,4-dihydroxybenzoate:[aryl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + 3,4-dihydroxybenzoate + holo-[aryl-carrier protein] = AMP + diphosphate + 3,4-dihydroxybenzoyl-[aryl-carrier protein] (overall reaction) [RN:
R12575 ];
(1a) ATP + 3,4-dihydroxybenzoate = diphosphate + (3,4-dihydroxybenzoyl)adenylate [RN:
R12576 ];
(1b) (3,4-dihydroxybenzoyl)adenylate + holo-[aryl-carrier protein] = AMP + 3,4-dihydroxybenzoyl-[aryl-carrier protein] [RN:
R12577 ]
Reaction(KEGG)
Substrate
ATP [CPD:
C00002 ];
3,4-dihydroxybenzoate [CPD:
C00230 ];
holo-[aryl-carrier protein] [CPD:
C20665 ];
(3,4-dihydroxybenzoyl)adenylate [CPD:
C20884 ]
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
3,4-dihydroxybenzoyl-[aryl-carrier protein] [CPD:
C20883 ];
(3,4-dihydroxybenzoyl)adenylate [CPD:
C20884 ]
Comment
The adenylation domain of the enzyme catalyses the activation of 3,4-dihydroxybenzoate to (3,4-dihydroxybenzoyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of an aryl-carrier protein domain. The aryl-carrier protein domain may be part of the same protein, or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.62 created 2020
Pathway
ec01053 Biosynthesis of siderophore group nonribosomal peptides
Orthology
K24110 3,4-dihydroxybenzoate---[aryl-carrier protein] ligase
Genes
AMIH : CO731_00651(fadD_1)
BAH : BAMEG_2607 BAMEG_2608(asbC)
PALR : HGI30_15375 HGI30_17890
CCHL : FPL14_04890 FPL14_24320
» show all
Taxonomy
Reference
Authors
Pfleger BF, Lee JY, Somu RV, Aldrich CC, Hanna PC, Sherman DH
Title
Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.62
ExPASy - ENZYME nomenclature database: 6.2.1.62