KEGG   ENZYME: 6.2.1.67
Entry
EC 6.2.1.67                 Enzyme                                 

Name
L-alanine---[L-alanyl-carrier protein] ligase;
ambB (gene name);
phsB (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
L-alanine:[L-alanyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-alanine + holo-[L-alanyl-carrier protein] = AMP + diphosphate + L-alanyl-[L-alanyl-carrier protein] (overall reaction);
(1a) ATP + L-alanine = diphosphate + (L-alanyl)adenylate [RN:R12725];
(1b) (L-alanyl)adenylate + holo-[L-alanyl-carrier protein] = AMP + L-alanyl-[L-alanyl-carrier protein]
Reaction(KEGG)
R12725;
(other) R12726 R12727
Substrate
ATP [CPD:C00002];
L-alanine [CPD:C00041];
holo-[L-alanyl-carrier protein];
(L-alanyl)adenylate [CPD:C22363]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-alanyl-[L-alanyl-carrier protein];
(L-alanyl)adenylate [CPD:C22363]
Comment
The adenylation domain of the enzyme catalyses the activation of L-alanine to (L-alanyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. The peptidyl-carrier protein domain may be part of the same protein, or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.67 created 2021
Orthology
K12912  phosphinothricin tripeptide synthetase phsB
K25233  L-alanine---[L-alanyl-carrier protein] ligase
Genes
PAE: PA2305(ambB)
PAEV: N297_2378(lgrD)
PAEI: N296_2378(lgrD)
PAU: PA14_34810(mxaA)
PAG: PLES_29991
PAF: PAM18_2735
PNC: NCGM2_3315(mxaA)
PAEB: NCGM1900_4225
PDK: PADK2_13745
PSG: G655_13525
PAEP: PA1S_14095
PAEM: U769_13690
PAEL: T223_15325
PAEG: AI22_19760
PAEC: M802_2375
PAEO: M801_2377
SECH: B18_23600
 » show all
Reference
1  [PMID:16251301]
  Authors
Schwartz D, Grammel N, Heinzelmann E, Keller U, Wohlleben W.
  Title
Phosphinothricin tripeptide synthetases in Streptomyces viridochromogenes Tu494.
  Journal
Antimicrob Agents Chemother 49:4598-607 (2005)
DOI:10.1128/AAC.49.11.4598-4607.2005
  Sequence
Reference
2  [PMID:25814981]
  Authors
Rojas Murcia N, Lee X, Waridel P, Maspoli A, Imker HJ, Chai T, Walsh CT, Reimmann C.
  Title
The Pseudomonas aeruginosa antimetabolite L -2-amino-4-methoxy-trans-3-butenoic acid (AMB) is made from glutamate and two alanine residues via a thiotemplate-linked tripeptide precursor.
  Journal
Front Microbiol 6:170 (2015)
DOI:10.3389/fmicb.2015.00170
  Sequence
[pae:PA2305]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.67
IUBMB Enzyme Nomenclature: 6.2.1.67
ExPASy - ENZYME nomenclature database: 6.2.1.67
BRENDA, the Enzyme Database: 6.2.1.67

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