KEGG   ENZYME: 6.2.1.69
Entry
EC 6.2.1.69                 Enzyme                                 
Name
L-cysteine---[L-cysteinyl-carrier protein] ligase;
pchE (gene name);
pchF (gene name);
angR (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
L-cysteine:[L-cysteinyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-cysteine + holo-[L-cysteinyl-carrier protein] = AMP + diphosphate + L-cysteinyl-[L-cysteinyl-carrier protein] (overall reaction);
(1a) ATP + L-cysteine = diphosphate + (L-cysteinyl)adenylate [RN:R12765];
(1b) (L-cysteinyl)adenylate + holo-[L-cysteinyl-carrier protein] = AMP + L-cysteinyl-[L-cysteinyl-carrier protein]
Reaction(KEGG)
R12765;
(other) R12766 R12767
Substrate
ATP [CPD:C00002];
L-cysteine [CPD:C00097];
holo-[L-cysteinyl-carrier protein];
(L-cysteinyl)adenylate [CPD:C22385]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-cysteinyl-[L-cysteinyl-carrier protein];
(L-cysteinyl)adenylate [CPD:C22385]
Comment
The adenylation domain of the enzyme catalyses the activation of L-cysteine to (L-cysteinyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. The peptidyl-carrier protein domain may be part of the same protein, or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.69 created 2021
Pathway
ec01053  Biosynthesis of siderophore group nonribosomal peptides
Orthology
K12239  L-cysteine---[L-cysteinyl-carrier protein] ligase PchE
K12240  L-cysteine---[L-cysteinyl-carrier protein] ligase PchF
Genes
KMIVW41_14180
PGELG71_13430 LG71_26195
SYMK6K13_09575
YPEYPO0776
YPAYPA_3374
YPNYPN_3216
YPMYP_3425(entF3)
YPZYPZ3_0722
YPTA1122_01155
YPDYPD4_0678
YPXYPD8_0678
YPWCH59_1087
YPJCH55_3520
YPVBZ15_2798
YPLCH46_140
YPSYPTB3296
YPOBZ17_3313
YPIYpsIP31758_0684
YPYYPK_0752
YPBYPTS_3434
YPQDJ40_3145
YPUBZ21_2577
YPRBZ20_2822
YPCBZ23_2860
YPFBZ19_2700
YSIBF17_03615
YFRAW19_774
YKRCH54_888
PCACOI450_11540
PAOPat9b_4773
PTTVY86_04720
XBVXBW1_1484
LANHKR767_17535
PAEPA4225(pchF) PA4226(pchE)
PAEVN297_4357 N297_4358
PAEIN296_4357 N296_4358
PAUPA14_09270(pchE) PA14_09280(pchF) PA14_54920
PAPPSPA7_0874(pchE) PSPA7_0875(pchF)
PAGPLES_07011(pchE) PLES_07021(pchF)
PAFPAM18_0711(pchE) PAM18_0712(pchF)
PNCNCGM2_5470(pchF) NCGM2_5471(pchE)
PAEBNCGM1900_0733(pchE) NCGM1900_0734(pchF)
PDKPADK2_03540 PADK2_03545
PSGG655_03575 G655_03580
PRPM062_22260 M062_22265
PAEPPA1S_03690 PA1S_03695
PAERPA1R_gp2116 PA1R_gp2117
PAEMU769_03720 U769_03725
PAELT223_03620 T223_03625
PAESSCV20265_0735 SCV20265_0736
PAEUBN889_04699(pchF) BN889_04700(pchE_1) BN889_04701(pchE_2)
PAEGAI22_00215 AI22_00220
PAECM802_4355 M802_4356
PAEOM801_4223 M801_4224
PPAAB7D75_03630
PFLPFL_3492(pchF) PFL_3493(pchE)
PPRCPFLCHA0_c35330(irp1) PFLCHA0_c35340(irp2)
PPROPPC_3646(pchF) PPC_3647(pchE)
PFBVO64_5522
PVRPverR02_15520 PverR02_15525
PAZOAYR47_21960
PFWPF1751_v1c25800
PFFPFLUOLIPICF724130
PCZPCL1606_27860 PCL1606_27870
PSEMTO66_16985 TO66_16990
PSOSPOS17_3635(pchF) POS17_3636(pchE)
PSEPC4K39_5485 C4K39_5486
PSAMHU731_027795
PZAHU749_012685
EMODM558_05695
PSENPNC201_20480(mbtB)
MIIBTJ40_11110
MECQ7C_386
MPURMARPU_03050
ADIB5T_02512
CHROCXB49_08490
AQLBXU06_11810
RSORSc1811
RSLRPSI07_1867
RSMCMR15_11817
RSEF504_1578
RPUCDC45_09320
BPSBPSS0586(pchE) BPSS0587(pchF)
BPMBURPS1710b_A2150(pchE) BURPS1710b_A2151(pchF)
BPLBURPS1106A_A0787 BURPS1106A_A0788
BPDBURPS668_A0877 BURPS668_A0878
BPSEBDL_3815 BDL_3816
BPSMBBQ_5606 BBQ_5607
BPSUBBN_3987 BBN_3988
BPSDBBX_5652 BBX_5653
BPZBP1026B_II0646(pchE) BP1026B_II0647(pchF)
BPQBPC006_II0832 BPC006_II0833
BPKBBK_3903 BBK_3904
BPSHDR55_4763 DR55_4764
BPSABBU_5458 BBU_5459
BPSOX996_3767 X996_3768
BUTX994_4031 X994_4032
BTEBTH_II1827 BTH_II1828(pchE)
BTQBTQ_5111 BTQ_5112
BTJBTJ_3739 BTJ_3740
BTZBTL_4597 BTL_4598
BTVBTHA_5631 BTHA_5632
BTHEBTN_4236 BTN_4237
BTHMBTRA_3856 BTRA_3857
BTHADR62_4285 DR62_4286
BTHLBG87_4568 BG87_4569
BURBcep18194_B0672 Bcep18194_B0673
BCMBcenmc03_5289 Bcenmc03_5290
BCHBcen2424_4997 Bcen2424_4998
BCJBCAM2230(pchE)
BCEOI35_6120(pchF) I35_6121(pchE)
BCEDDM42_5764 DM42_5765
BCEPAPZ15_27910 APZ15_27915
BSEMWJ12_31050 WJ12_31055
BSTLBBJ41_24870 BBJ41_24875
BARINLX30_26105 NLX30_26110
BGDbgla_4p2140
BGPBGL_1c20570(pchF) BGL_1c20580(pchE)
BPLAbpln_1g19090 bpln_1g19100
OTDJ1M35_10305
HRBHrubri_2237
DEOCAY53_00285
SCUSCE1572_43415
BRKCWS35_16345
RPCRPC_3032
SECHB18_04270 B18_04275
ALIAZOLI_p20160(pchE) AZOLI_p20161(pchF)
HJOAY555_10640
LYBC3943_18865
LPAKGDS87_10285
LMACI6G82_03570
BLRBRLA_c037020
BFMBP422_17845
BAGRBA6348_08945
BRWGOP56_10725
SANCSANR_1291
CKLCKL_1511
CKRCKR_1405
CSBCLSA_c16940(irp2)
CLDCLSPO_c23060
CAUNCLAUR_026160(dltA_6)
RALRumal_0885
BPROPMF13cell1_00657(mbtB_1)
ACACEYQ97_15755
EHLEHLA_1441
RTORTO_11580 RTO_12320
CBOLCGC65_09170
SINTK5I26_14510
DSYDSY2821
DHDDhaf_3965
DAEDtox_2674
VGUHYG85_16695
AVGI6H45_03220
PFTJBW_03609 JBW_03612
MANAMAMMFC1_02828(mbtB_2)
STEDSPTER_12030(mbtB_1)
MPAMAP_3742
MAVIRC58_00135
MAVURE97_00135
MPAGC0J29_21250
CDHCDB402_1084(irp2C)
CDZCD31A_2083(irp2C)
CDSCDC7B_2042(irp2C)
CDDCDCE8392_1964(irp2C)
CDIPERS451417_02094(irp2C)
CSXCSING_01210(irp2C)
CSPWM42_1816
NFRERS450000_00763(mbtB_1)
NCYNOCYR_2585
NNONONO_c49320
NODFOH10_15025
NWLNWFMUON74_24310
NIEKV110_22875 KV110_22880 KV110_35845
CBQAL705_04305
SCOSCO7683(SC4C2.18)
SCBSCAB_1471 SCAB_1481
SSXSACTE_0740
SRCM271_09930 M271_09960
SVESVEN_0512 SVEN_0517
SLVSLIV_00885
SGUSGLAU_04465(nrps1B) SGLAU_04470(nrps1C) SGLAU_04640(nrps1D) SGLAU_04650(nrps1E)
STREGZL_p00202
SPHWNFX46_19160 NFX46_19185
SLSSLINC_1332
STRDNI25_03970
SNQCP978_29875
SPADDVK44_09635 DVK44_09640
SPACB1H29_04830
SHUNDWB77_06824(mbtB_1)
SHARHUT13_23935
SFEUIM697_24270
SROIIAG44_38705
SDURM4V62_41900
YIALO772_01875
CMICMM_0330(npsB)
CMCCMN_00301(npsB)
CMHVO01_01490
CCAFFGD68_03585
DCOSAMEA4475696_1592(mbtB_3)
PACDEGX94_02945
PAUSNCTC13651_00740(mbtB_1)
THAONI17_005435
TALXFOF52_04705
NDANdas_1030 Ndas_1761
NEXNE857_12315
SNAHOUQ99_18135
ACTWF7P10_30275
SROSros_3767
SACGFDZ84_24490
SACCEYD13_12025(mbtB1)
AORISD37_07330 SD37_10150
PHHAFB00_19555
PAUTPdca_37550
AHMTL08_14435
ACTUActkin_03287(angR)
STPStrop_2820
VMAVAB18032_07015
MCRAID554_27815
VERHUT12_19180
ASEACPL_6139(nrps2A) ACPL_6158(nrps2C)
ACTSACWT_6007 ACWT_6026
PRYPrubr_62260
EYYEGYY_00410(EntF) EGYY_29280(EntF)
AEQAEQU_0072 AEQU_0074
AHATADCFC_02020
 » show all
Reference
1  [PMID:10555976]
  Authors
Quadri LE, Keating TA, Patel HM, Walsh CT
  Title
Assembly of the Pseudomonas aeruginosa nonribosomal peptide siderophore pyochelin: In vitro reconstitution of aryl-4, 2-bisthiazoline synthetase activity from PchD, PchE, and PchF.
  Journal
Biochemistry 38:14941-54 (1999)
DOI:10.1021/bi991787c
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.69
IUBMB Enzyme Nomenclature: 6.2.1.69
ExPASy - ENZYME nomenclature database: 6.2.1.69
BRENDA, the Enzyme Database: 6.2.1.69

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