KEGG   ENZYME: 6.2.1.72
Entry
EC 6.2.1.72                 Enzyme                                 

Name
L-serine---[L-seryl-carrier protein] ligase;
entF (gene name);
zmaJ (gene name);
gdnB (gene name);
serine-activating enzyme
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
L-serine:[L-seryl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-serine + holo-[L-seryl-carrier protein] = AMP + diphosphate + L-seryl-[L-seryl-carrier protein] (overall reaction);
(1a) ATP + L-serine = diphosphate + (L-seryl)adenylate [RN:R12779];
(1b) (L-seryl)adenylate + holo-[L-seryl-carrier protein] = AMP + L-seryl-[L-seryl-carrier protein]
Reaction(KEGG)
R12779;
(other) R12780 R12781
Substrate
ATP [CPD:C00002];
L-serine [CPD:C00065];
holo-[L-seryl-carrier protein];
(L-seryl)adenylate [CPD:C05820]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-seryl-[L-seryl-carrier protein];
(L-seryl)adenylate [CPD:C05820]
Comment
The adenylation domain of the enzyme catalyses the activation of L-serine to (L-seryl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. The peptidyl-carrier protein domain may be part of the same protein, or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.72 created 2021
Pathway
ec01053  Biosynthesis of siderophore group nonribosomal peptides
Orthology
K02364  L-serine---[L-seryl-carrier protein] ligase
Genes
ECO: b0586(entF)
ECJ: JW0578(entF)
ECD: ECDH10B_0546(entF) ECDH10B_0654(entF)
EBW: BWG_0459(entF)
ECOK: ECMDS42_0447(entF)
ECE: Z0727(entF)
ECS: ECs0625(entF)
ECF: ECH74115_0671(entF)
ETW: ECSP_0640(entF)
ELX: CDCO157_0610(entF)
EOI: ECO111_0616(entF)
EOJ: ECO26_0661(entF)
EOH: ECO103_0594(entF)
ECOO: ECRM13514_0608(entF)
ECOH: ECRM13516_0552(entF)
ESL: O3K_18660(entF)
ESO: O3O_06635(entF)
ESM: O3M_18640(entF)
ECK: EC55989_0578(entF)
ECG: E2348C_0488(entF)
EOK: G2583_0749(entF)
ELR: ECO55CA74_03670(entF)
ELH: ETEC_0616
ECW: EcE24377A_0606(entF)
EUN: UMNK88_619(entF)
ECP: ECP_0618
ENA: ECNA114_0529(entF)
ECOS: EC958_0706(entF)
ECV: APECO1_1463(entF)
ECOA: APECO78_06510(entF)
ECX: EcHS_A0636(entF)
ECM: EcSMS35_0606(entF)
ECY: ECSE_0653
ECR: ECIAI1_0570(entF)
ECQ: ECED1_0582(entF)
EUM: ECUMN_0680(entF)
ECT: ECIAI39_0563(entF)
EOC: CE10_0586(entF)
EBR: ECB_00553(entF)
EBL: ECD_00553(entF)
EBE: B21_00542(entF)
EBD: ECBD_3069
ECI: UTI89_C0588(entF)
EIH: ECOK1_0598(entF)
ECZ: ECS88_0625(entF)
ECC: c0673(entF)
ELO: EC042_0624(entF)
ELN: NRG857_02655(entF)
ESE: ECSF_0527
EKF: KO11_20735(entF)
EAB: ECABU_c06360(entF)
EDJ: ECDH1ME8569_0556(entF)
ELU: UM146_14575(entF)
ELW: ECW_m0641(entF)
ELL: WFL_03185(entF)
ELC: i14_0647(entF)
ELD: i02_0647(entF)
ELP: P12B_c0571(entF)
ELF: LF82_0568(entF)
ECOL: LY180_03180(entF)
ECOI: ECOPMV1_00604(entF)
ECOJ: P423_02875(entF)
EFE: EFER_2508(entF)
EAL: EAKF1_ch0875c(entF)
EMA: C1192_09780(entF)
ESZ: FEM44_17365(entF)
STY: STY0631(entF)
STT: t2280(entF)
SENT: TY21A_11570(entF)
STM: STM0588(entF)
SEO: STM14_0686(entF)
SEY: SL1344_0576(entF)
SEM: STMDT12_C06500(entF)
SEJ: STMUK_0593(entF)
SEB: STM474_0608(entF)
SEF: UMN798_0637(entF)
SETU: STU288_11435(entF)
SETC: CFSAN001921_14095(entF)
SENR: STMDT2_05791(entF)
SEND: DT104_06161(entF)
SENI: CY43_03225(entF)
SEEN: SE451236_08960(entF)
SPT: SPA2146(entF)
SEK: SSPA1996
SEI: SPC_0600(entF)
SEC: SCH_0619(entF)
SHB: SU5_01278
SENH: CFSAN002069_05905(entF)
SEEH: SEEH1578_12370(entF)
SENS: Q786_02870(entF)
SED: SeD_A0684
SEG: SG0592(entF)
SEL: SPUL_2378(entF)
SEGA: SPUCDC_2364(entF)
SET: SEN0557(entF)
SENA: AU38_02850(entF)
SENO: AU37_02845(entF)
SENV: AU39_02850(entF)
SENQ: AU40_03135(entF)
SENL: IY59_02905(entF)
SEEC: CFSAN002050_09550(entF)
SEEB: SEEB0189_016340(entF)
SENB: BN855_5800
SENE: IA1_03085(entF)
SENC: SEET0819_09905(entF)
SBG: SBG_0496(entF)
SBZ: A464_561
SALZ: EOS98_16185(entF)
SFL: SF0498(entF)
SFX: S0504(entF)
SFV: SFV_0532(entF)
SFE: SFxv_0552(entF)
SFN: SFy_0646
SFS: SFyv_0686
SFT: NCTC1_00519(entF)
SSN: SSON_0537(entF)
SBO: SBO_0447(entF)
SBC: SbBS512_E0488(entF)
SDY: SDY_0515(entF)
SHQ: A0259_06610(entF)
ENC: ECL_03114(entF)
ENL: A3UG_05990(entF)
ECLE: ECNIH2_06650(entF)
ECLI: ECNIH5_05770(entF)
ECLX: LI66_05640(entF)
ECLY: LI62_06550(entF)
ECLZ: LI64_06145(entF)
ECLO: ENC_21460
EHM: AB284_19240(entF)
EXF: BFV63_05770(entF)
ECLA: ECNIH3_05780(entF)
ECLC: ECR091_05760(entF)
EAU: DI57_12880(entF)
EEC: EcWSU1_01162(entF)
ELG: BH714_01925(entF)
ECAN: CWI88_16475(entF)
ERN: BFV67_05470(entF)
ECLS: LI67_006740(entF)
ECHG: FY206_07055(entF)
ESH: C1N69_05815(entF)
ENR: H650_21565(entF)
ENX: NI40_005485(entF)
ENF: AKI40_3702(entF)
LNI: CWR52_06485(entF)
EBG: FAI37_00750(entF)
END: A4308_09790(entF)
ESA: ESA_02727
CSK: ES15_2797(entF)
CSZ: CSSP291_12860(entF)
CCON: AFK62_05635(entF)
CDM: AFK67_05840(entF)
CMJ: AFK66_014070(entF)
CUI: AFK65_05615(entF)
CMW: AFK63_13045(entF)
CTU: CTU_12350(entF)
KPN: KPN_00605(entF)
KPU: KP1_1550(entF)
KPP: A79E_3635
KPH: KPNIH24_21605(entF)
KPZ: KPNIH27_06670(entF)
KPV: KPNIH29_07360(entF)
KPW: KPNIH30_07400(entF)
KPY: KPNIH31_07275(entF)
KPG: KPNIH32_07505(entF)
KPC: KPNIH10_07185(entF)
KPQ: KPR0928_07095(entF)
KPT: VK055_1931(entF)
KPO: KPN2242_05885(entF)
KPJ: N559_3721
KPI: D364_03175(entF)
KPX: PMK1_02936(entF)
KPB: FH42_24055(entF)
KPNE: KU54_019255(entF)
KPNU: LI86_19080(entF)
KPNK: BN49_1661(entF)
KVA: Kvar_3763
KPE: KPK_3973(entF)
KVD: KR75_14940(entF)
KVQ: SP68_21570(entF)
KOX: KOX_13905(entF)
KOE: A225_1600
KOY: J415_23630(entF)
KOM: HR38_12650(entF)
KOK: KONIH1_07855(entF)
KOC: AB185_28265(entF)
KQU: AVR78_11690(entF)
EAE: EAE_13615(entF)
EAR: CCG31248
KQV: B8P98_20660(entF)
KLL: BJF97_08240(entF)
KLW: DA718_20495(entF)
ROR: RORB6_12060(entF)
RON: TE10_08850(entF)
RPLN: B1209_19755(entF)
RAO: DSD31_18605(entF)
RTG: NCTC13098_05440(entF_1) NCTC13098_05441(entF_2)
REE: electrica_03713(entF)
CRO: ROD_05961(entF)
CKO: CKO_02575
CFD: CFNIH1_13030(entF)
CBRA: A6J81_10160(entF)
CWE: CO701_15835(entF)
CYO: CD187_17305(entF)
CPOT: FOB25_04795(entF)
CFQ: C2U38_06880(entF)
CAMA: F384_02585(entF)
CAF: AL524_19705(entF)
CIF: AL515_07590(entF)
CFAR: CI104_07295(entF)
CIR: C2U53_16490(entF)
CIE: AN232_23395(entF)
CPAR: CUC49_02720(entF)
CTEL: GBC03_23000(entF)
CITZ: E4Z61_10660(entF)
CARS: E1B03_08095(entF)
EBT: EBL_c13840(entF)
PGE: LG71_22020(entF)
KLE: AO703_06205(entF)
KSA: C813_08900(entF)
KOR: AWR26_18325(entF)
KRD: A3780_05955(entF)
KCO: BWI95_11715(entF)
KOT: EH164_16375(entF)
KPSE: IP581_05990(entF)
KIE: NCTC12125_02379(entF_1) NCTC12125_02380(entF_2)
KAS: KATP_32740(entF)
LAX: APT61_16660(entF)
LEI: C2U54_10800(entF)
LEH: C3F35_05670(entF)
LEE: DVA44_17800(entF)
LER: GNG29_06015(entF)
LEA: GNG26_05695(entF)
LAZ: A8A57_05885(entF)
LEW: DAI21_20920(entF)
METY: MRY16398_41550(entF)
AHN: NCTC12129_03608(entF_1)
YRE: HEC60_00225(entF)
SGOE: A8O29_016635(entF)
KIN: AB182_18220(entF)
PDZ: HHA33_20765(entF)
EBF: D782_3252
EBC: C2U52_29500(entF)
EBU: CUC76_08380(entF)
SMAR: SM39_4660 SM39_4670(entF)
SRL: SOD_c08290(dhbF1) SOD_c33480(entF)
SPLY: Q5A_004410(dhbF_1) Q5A_018140(entF)
SMAF: D781_3213
SERF: L085_02490(entF) L085_02540 L085_10400(entF)
SERM: CLM71_16985(entF)
SFO: Z042_25835(entF)
EGE: EM595_0490(entF)
PVA: Pvag_3663(entF)
PAGG: AL522_06445(entF)
KLN: LH22_19795(entF)
TCI: A7K98_01195(entF)
TPTY: NCTC11468_00309(entF)
PAY: PAU_00883(phbG)
XCC: XCC3867(entF)
XCB: XC_3952
XCP: XCR_0420
XAX: XACM_3816
XAC: XAC3922(entF)
XCI: XCAW_00356(entF)
XPH: XppCFBP6546_14790(XppCFBP6546P_14790)
SML: Smlt2818(entF)
SMT: Smal_2272
SMZ: SMD_2464(entF)
SINC: DAIF1_24160(dhbF)
LAB: LA76x_0319(dhbF)
LAQ: GLA29479_4135(dhbF)
LCP: LC55x_5004(dhbF)
LGU: LG3211_4985(dhbF)
LEZ: GLE_4894
LEM: LEN_4535
VCH: VC_1579
VTA: B1382
POR: APT59_14295(entF)
PSYR: N018_14090
PEN: PSEEN2342
PPSL: BJP27_05275(entF)
METL: U737_11050
MMAI: sS8_5016
ABO: ABO_1784
AVR: B565_2633
CHAE: CH06BL_14950(entF)
LMIR: NCTC12852_01585(dhbF)
PUT: PT7_0941
AFQ: AFA_01440
ARA: Arad_8972(vbsS)
AVI: Avi_3342
BME: BMEII0076
BMEL: DK63_3169
BMEE: DK62_2230
BMF: BAB2_0016
BABO: DK55_3126
BABR: DO74_2425
BABT: DK49_2129
BABB: DK48_2671
BABU: DK53_3113
BABS: DK51_2598
BABC: DO78_3044
BMS: BRA0017
BSZ: DK67_2052
BOV: BOV_A0014
BCS: BCAN_B0019(tycC)
BCAR: DK60_2907
BCAS: DA85_10605
BMR: BMI_II17(entF)
BPP: BPI_II17(entF)
BPV: DK65_2439
BVL: BF3285c2_0781(entF1)
BIO: BR141012304_20996(entF)
SNO: Snov_3330
LABT: FIU93_28955(dhbF1)
BVY: NCTC9239_02597(dhbF)
KVL: KVU_1314(vbsS)
KVU: EIO_1848
KRO: BVG79_01514(dhbF)
CID: P73_2107(entF)
SPSE: SULPSESMR1_03056(dhbF)
PAMN: JCM7685_0408(entF1) JCM7685_2850(entF3)
RSU: NHU_04515
PALW: PSAL_031170(dhbF)
ASZ: ASN_1062(entF)
TMO: TMO_0595(dhbF)
CPHO: CPHO_11125
CAQU: CAQU_04490
CPEG: CPELA_09420(entF)
CGK: CGERO_09140(entF)
CPSO: CPPEL_09595(entF)
CCYS: SAMEA4530656_2119(nrpS4)
NAD: NCTC11293_06435(entF)
NWL: NWFMUON74_31280(entF)
ROP: ROP_04690
GBR: Gbro_0642
SMAL: SMALA_3025
SBH: SBI_09312
SVE: SVEN_7440
SALU: DC74_1183
SALL: SAZ_06410
STRE: GZL_07306
SLD: T261_7328
SALJ: SMD11_1311(entF)
SFK: KY5_1051c
MPH: MLP_27470
GAU: GAU_3218
 » show all
Reference
1  [PMID:3040679]
  Authors
Pettis GS, McIntosh MA
  Title
Molecular characterization of the Escherichia coli enterobactin cistron entF and  coupled expression of entF and the fes gene.
  Journal
J Bacteriol 169:4154-62 (1987)
DOI:10.1128/jb.169.9.4154-4162.1987
Reference
2  [PMID:1826089]
  Authors
Rusnak F, Sakaitani M, Drueckhammer D, Reichert J, Walsh CT
  Title
Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine.
  Journal
Biochemistry 30:2916-27 (1991)
DOI:10.1021/bi00225a027
  Sequence
[eco:b0586]
Reference
3  [PMID:1338974]
  Authors
Reichert J, Sakaitani M, Walsh CT.
  Title
Characterization of EntF as a serine-activating enzyme.
  Journal
Protein Sci 1:549-56 (1992)
DOI:10.1002/pro.5560010410
Reference
4  [PMID:10688898]
  Authors
Ehmann DE, Shaw-Reid CA, Losey HC, Walsh CT.
  Title
The EntF and EntE adenylation domains of Escherichia coli enterobactin synthetase: sequestration and selectivity in acyl-AMP transfers to thiolation domain cosubstrates.
  Journal
Proc Natl Acad Sci U S A 97:2509-14 (2000)
DOI:10.1073/pnas.040572897
Reference
5  [PMID:16983083]
  Authors
Chan YA, Boyne MT 2nd, Podevels AM, Klimowicz AK, Handelsman J, Kelleher NL, Thomas MG.
  Title
Hydroxymalonyl-acyl carrier protein (ACP) and aminomalonyl-ACP are two additional type I polyketide synthase extender units.
  Journal
Proc Natl Acad Sci U S A 103:14349-54 (2006)
DOI:10.1073/pnas.0603748103
Reference
6  [PMID:18704088]
  Authors
Frueh DP, Arthanari H, Koglin A, Vosburg DA, Bennett AE, Walsh CT, Wagner G.
  Title
Dynamic thiolation-thioesterase structure of a non-ribosomal peptide synthetase.
  Journal
Nature 454:903-6 (2008)
DOI:10.1038/nature07162
  Sequence
[eco:b0586]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.72
IUBMB Enzyme Nomenclature: 6.2.1.72
ExPASy - ENZYME nomenclature database: 6.2.1.72
BRENDA, the Enzyme Database: 6.2.1.72

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