KEGG   ENZYME: 6.2.1.73
Entry
EC 6.2.1.73                 Enzyme                                 

Name
L-tryptophan---[L-tryptophyl-carrier protein] ligase;
ecm13 (gene name);
swb11 (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
L-tryptophan:[L-tryptophyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-tryptophan + holo-[L-tryptophyl-carrier protein] = AMP + diphosphate + -L-tryptophyl-[L-tryptophyl-carrier protein] (overall reaction);
(1a) ATP + tryptophan = diphosphate + (L-tryptophyl)adenylate [RN:R12782];
(1b) (L-tryptophyl)adenylate + holo-[L-tryptophyl-carrier protein] = AMP + L-tryptophyl-[L-tryptophyl-carrier protein]
Reaction(KEGG)
R12782;
(other) R12783 R12784
Substrate
ATP [CPD:C00002];
L-tryptophan [CPD:C00078];
holo-[L-tryptophyl-carrier protein];
tryptophan [CPD:C00078];
(L-tryptophyl)adenylate [CPD:C22391]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
-L-tryptophyl-[L-tryptophyl-carrier protein];
(L-tryptophyl)adenylate [CPD:C22391];
L-tryptophyl-[L-tryptophyl-carrier protein]
Comment
The adenylation domain of the enzyme catalyses the activation of L-tryptophan to (L-tryptophyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. The peptidyl-carrier protein domain may be part of the same protein, or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.73 created 2021
Orthology
K25254  L-tryptophan---[L-tryptophyl-carrier protein] ligase
Genes
SGU: SGLAU_04475
SCLF: BB341_00365
SPUN: BFF78_15605
SALF: SMD44_06523
SSPO: DDQ41_25595
SPAD: DVK44_09690
KQI: F1D05_02770
SSYI: EKG83_25950
Reference
1  [PMID:23437232]
  Authors
Zhang C, Kong L, Liu Q, Lei X, Zhu T, Yin J, Lin B, Deng Z, You D.
  Title
In vitro characterization of echinomycin biosynthesis: formation and hydroxylation of L-tryptophanyl-S-enzyme and oxidation of (2S,3S) beta-hydroxytryptophan.
  Journal
PLoS One 8:e56772 (2013)
DOI:10.1371/journal.pone.0056772
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.73
IUBMB Enzyme Nomenclature: 6.2.1.73
ExPASy - ENZYME nomenclature database: 6.2.1.73
BRENDA, the Enzyme Database: 6.2.1.73

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