KEGG   ENZYME: 6.2.2.1
Entry
EC 6.2.2.1                  Enzyme                                 

Name
thioglycine synthase;
ycaO (gene name) (ambiguous)
Class
Ligases;
Forming carbon-sulfur bonds;
Amide---thiol ligases
Sysname
[methyl-coenzyme M reductase]-glycine---sulfur ligase (thioglycine-forming)
Reaction(IUBMB)
ATP + sulfide + a [methyl-coenzyme M reductase]-glycine = ADP + phosphate + a [methyl-coenzyme M reductase]-thioglycine
Substrate
ATP [CPD:C00002];
sulfide [CPD:C00283];
[methyl-coenzyme M reductase]-glycine
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
[methyl-coenzyme M reductase]-thioglycine
Comment
Requires Mg2+. The enzyme is found in anaerobic methanogenic and methanotrophic archaea, where it modifies a glycine residue in EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase (methyl-CoM reductase). Upon binding to its substrate, an external source of sulfide attacks the target amide bond generating a tetrahedral intermediate. The amide oxyanion attacks the gamma-phosphate of ATP, releasing ADP and forming a phosphorylated thiolate intermediate that collapses to form thioglycine and phosphate. In most organisms activity requires a second protein (TfuA) , which may allosterically activate this enzyme or assist in the delivery of sulfide to the substrate.
History
EC 6.2.2.1 created 2020
Reference
1  [PMID:28880150]
  Authors
Nayak DD, Mahanta N, Mitchell DA, Metcalf WW
  Title
Post-translational thioamidation of methyl-coenzyme M reductase, a key enzyme in  methanogenic and methanotrophic Archaea.
  Journal
Elife 6 (2017)
  Sequence
Reference
2  [PMID:29507203]
  Authors
Mahanta N, Liu A, Dong S, Nair SK, Mitchell DA
  Title
Enzymatic reconstitution of ribosomal peptide backbone thioamidation.
  Journal
Proc Natl Acad Sci U S A 115:3030-3035 (2018)
  Sequence
[mac:MA_0165 MA_0164] [mka:MK0115]
Reference
3  [PMID:31139720]
  Authors
Dong SH, Liu A, Mahanta N, Mitchell DA, Nair SK
  Title
Mechanistic Basis for Ribosomal Peptide Backbone Modifications.
  Journal
ACS Cent Sci 5:842-851 (2019)
  Sequence
[mja:MJ_1094]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.2.1
IUBMB Enzyme Nomenclature: 6.2.2.1
ExPASy - ENZYME nomenclature database: 6.2.2.1
BRENDA, the Enzyme Database: 6.2.2.1

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