EC 22.214.171.124 Enzyme
ycaO (gene name) (ambiguous)
Forming carbon-sulfur bonds;
[methyl-coenzyme M reductase]-glycine---sulfur ligase (thioglycine-forming)
ATP + sulfide + a [methyl-coenzyme M reductase]-glycine = ADP + phosphate + a [methyl-coenzyme M reductase]-thioglycine
[methyl-coenzyme M reductase]-glycine
[methyl-coenzyme M reductase]-thioglycine
Requires Mg2+. The enzyme is found in anaerobic methanogenic and methanotrophic archaea, where it modifies a glycine residue in EC
, coenzyme-B sulfoethylthiotransferase (methyl-CoM reductase). Upon binding to its substrate, an external source of sulfide attacks the target amide bond generating a tetrahedral intermediate. The amide oxyanion attacks the gamma-phosphate of ATP, releasing ADP and forming a phosphorylated thiolate intermediate that collapses to form thioglycine and phosphate. In most organisms activity requires a second protein (TfuA) , which may allosterically activate this enzyme or assist in the delivery of sulfide to the substrate.
EC 126.96.36.199 created 2020
Nayak DD, Mahanta N, Mitchell DA, Metcalf WW
Post-translational thioamidation of methyl-coenzyme M reductase, a key enzyme in methanogenic and methanotrophic Archaea.
Elife 6 (2017)
Mahanta N, Liu A, Dong S, Nair SK, Mitchell DA
Enzymatic reconstitution of ribosomal peptide backbone thioamidation.
Proc Natl Acad Sci U S A 115:3030-3035 (2018)
Dong SH, Liu A, Mahanta N, Mitchell DA, Nair SK
Mechanistic Basis for Ribosomal Peptide Backbone Modifications.
ACS Cent Sci 5:842-851 (2019)
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