KEGG   ENZYME: 6.3.1.12
Entry
EC 6.3.1.12                 Enzyme                                 
Name
D-aspartate ligase;
Aslfm;
UDP-MurNAc-pentapeptide:D-aspartate ligase;
D-aspartic acid-activating enzyme
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)
Sysname
D-aspartate:[beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n ligase (ADP-forming)
Reaction(IUBMB)
ATP + D-aspartate + [beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n = [beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n + ADP + phosphate [RN:R09590]
Reaction(KEGG)
R09590
Substrate
ATP [CPD:C00002];
D-aspartate [CPD:C00402];
[beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n [CPD:C19695]
Product
[beta-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-6-N-(beta-D-Asp)-L-Lys-D-Ala-D-Ala)]n [CPD:C19696];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
This enzyme forms part of the peptidoglycan assembly pathway of Gram-positive bacteria grown in medium containing D-Asp. Normally, the side chains the acylate the 6-amino group of the L-lysine residue contain L-Ala-L-Ala but these amino acids are replaced by D-Asp when D-Asp is included in the medium. Hybrid chains containing L-Ala-D-Asp, L-Ala-L-Ala-D-Asp or D-Asp-L-Ala are not formed [4]. The enzyme belongs in the ATP-grasp protein superfamily [3,4]. The enzyme is highly specific for D-aspartate, as L-aspartate, D-glutamate, D-alanine, D-iso-asparagine and D-malic acid are not substrates [4]. In Enterococcus faecium, the substrate D-aspartate is produced by EC 5.1.1.13, aspartate racemase [4]
History
EC 6.3.1.12 created 2006
Orthology
K17810  D-aspartate ligase
Genes
LMBC9I47_2192
RHDR2APBS1_3073
RTHLRK53_15370
LPYFIV34_04795
THTE2K93_07160
SDESde_0164
HJABST95_00395
RHHE0Z06_08805
THIPN838_30085
THIMKFB96_07770
HHCM911_05695
APRSBI364_11655
WMAWM2015_2390
SDFACG33_04750
SALNSALB1_0089
EPSL0Y14_08265
CNCCNE_2c00200
BAMBamb_6484
BACBamMC406_1119
BGEBC1002_0557
PSPWBJG93_10125
RHGEXZ61_09950
UPVEJN92_09595
UPIEJG51_012490
SNNEWH46_02265 EWH46_02275 EWH46_02690
NLCEBAPG3_001255
EBAebA4298
PCAPcar_0630
PACEA6070_12575
PEFA7E78_03215
PPDPpro_2168
DBKDGMP_01180
DATHRM2_19890
DTOTOL2_C23170
DEKDSLASN_27270
MXAMXAN_2357
MSDMYSTI_02726
MYMA176_004686
MFBMFUL124B02_14985
CCXCOCOR_05710
MFULILAB_19490
MMASMYMAC_002325
AGEAA314_00657
AVMJQX13_13260
MBDMEBOL_003489
CFUSCYFUS_000124
SCUSCE1572_28190
CCROCMC5_002470
MRMA7982_05915
SFUSfum_0958
AGRAGROH133_12338
ATFAch5_36420
NHANham_0189
OCAOCAR_5670
OCGOCA5_c23360
OCOOCA4_c23350
METM446_1460
MNOMnod_6859
MHEYH2LOC_001225
CMAGCBW24_18010
SPHUSPPYR_1652
SMICSmB9_22590
AEPAMC99_02605
ALIAZOLI_p10609
ABSAZOBR_p150058
ABQABAZ39_21065
ABFAMK58_19970
ATIAL072_19760
AZTTSH58p_25200
AZMDM194_13535 DM194_14170
AOZHUE56_24400
SKTIGS68_12975
SMUCJL100_014220
SROEJL101_011065
BAYRBAM_037540
BAQBACAU_3760
BYABANAU_3930
BAMLBAM5036_3671
BAMARBAU_3874
BAMNBASU_3659
BAMBBAPNAU_3940
BAMTAJ82_21180
BAMYV529_40160
BMPNG74_03931
BAOBAMF_3868(RBAM_037540)
BAZBAMTA208_20405
BQLLL3_04196
BXHBAXH7_04185
BAMIKSO_000310
BAMCU471_39100
BAMFU722_19960
BSIACWD84_00465
BVMB9C48_19160
BMYCDJ92_517
BNTGSN03_27670
BDAFSZ17_01460 FSZ17_02605
BACPSB24_10015
BACBOY17_02440
BACOOXB_0273
BACSAUL54_11065
BCOHBC6307_22585
BMQBMQ_pBM60036
BMHBMWSH_p10034
BFXBC359_11945
OCNCUC15_02730
OCBCV093_02800
LSPBsph_0276 Bsph_1651 Bsph_4214
LGYT479_17810 T479_20575
LFUHR49_03685 HR49_06090 HR49_17980
LYSLBYS11_05525 LBYS11_17280 LBYS11_19795
LYBC3943_20290 C3943_23425
LYZDCE79_14420
LYGC1N55_17360
LPAKGDS87_18285 GDS87_20965
LAGRFJQ98_21985 FJQ98_22815
LMACI6G82_02235 I6G82_23225
HHDHBHAL_4977
HMNHM131_02520
HLIHLI_13120
VILCFK37_06100
VNECFK40_01590
SJEAAV35_012250
PSYHD0S48_19895
PSYOPB01_13000
MACRBHM04_09800
MBOEHT586_01125
MBRUMGG13_11180
PALBEJC50_17645
PRZGZH47_25620
PLYCGXP70_22045
PALOE6C60_2172
ASOCCB4_02475
COHEAV92_12470 EAV92_21205
SIVSSIL_1018
SSILSOLI23_13395
SOBCSE16_17060
PLNPlano_1114
PKUAUO94_10490
PRTAUC31_03440
PLLI858_001315
PANABBH88_14320
PDGBCM40_09895
PHCBBI08_02295
PMARB0X71_04640
PPLABBI15_02310
PFAEAJGP001_06780
PLXCW734_08050
PMATBBI11_07460
PDECH1Q58_06620
PGQFK545_14970
KURASO14_443
KZONCTC404_01309
SPSYAZE41_03490
SPORSporoP33_05395
SPOPSporoP37_05885
SURESporoP32a_05905
SPOSDV702_09595
SPAEE2C16_00320
SPOOJ3U78_09315
RSTATY39_11200
PAEKD3873_10530
PANCE2636_03840
PLAYDNR44_014175
VIJJNUCC6_11950
LLAL93420(yxbA)
LLKLLKF_2494(yxbA)
LLTCVCAS_2270(yxbA)
LLSlilo_2195(yxbA)
LLDP620_13190
LLXNCDO2118_2348
LLJLG36_2164(yxbA)
LLMllmg_2505
LLCLACR_2532
LLRllh_12965
LLNLLNZ_12940
LLIuc509_2198
LLWkw2_2286
LACTD7I46_09760
LACKFLP15_02180
LTIJW886_00800
LJOLJ_0136
LJFFI9785_203
LJHLJP_0142
LJNT285_00755
LACLBA0159
LADLA14_0159
LAFSD55_0156
LDBLdb0210 Ldb0307
LBULBUL_0184 LBUL_0265
LDELDBND_0201 LDBND_0269
LDLLBU_0164 LBU_0244
LGALGAS_0136
LHElhv_0165
LHLLBHH_0166
LHRR0052_00895
LHVlhe_0172
LHHLBH_0137
LHDHUO_01800
LCRLCRIS_00154
LAMLA2_00865
LAILAC30SC_00730
LAYLAB52_00810
LKEWANG_1714
LAWLACWKB8_1618
LAELBAT_0182
LGLAO203_02105
LJEBUE77_06145
LAMYB1745_00790
LPWLpgJCM5343_01370
LKLDKL58_08335
LAPIDKL56_07145
LHSDLD54_07715
LTAH1A07_00745
LUUH4B44_00895
LPAIGYM71_09235
LIDGYK47_05865
LCALSEI_2148
LCZLCAZH_2108
LCSLCBD_2316
LCELC2W_2298
LCWBN194_22880
LCLLOCK919_2327
LPQAF91_03020
LPILBPG_02069
LPAPLBPC_2084
LCBLCABL_23300(yxbA)
LCXLCA12A_0691
LRHLGG_02152(yxbA)
LRGLRHM_2069
LRLLC705_02159(yxbA)
LRALRHK_2161(yxbA)
LROLOCK900_2113
LRCLOCK908_2222
LCHIKG086_09460
LZEKG087_10060
LRELreu_1936
LRFLAR_1812
LRUHMPREF0538_21143
LRTLRI_1883
LRRN134_10265
LFELAF_1396
LFRLC40_0892
LFFLBFF_1517
LMULBLM1_00040
LORAYI71_11845
LVALV515_09240
LFNLF145_01505
LPONLP475_08565
LGASLG045_09285
LPASKZE55_02225
LLEAYR59_06085
LFVLF543_02700
FIXM8332_02925
LBHLbuc_1239
LBNLBUCD034_1363
LPARFAM21731_02225
LCUPL11_008345
LKFDNL43_10650
LHILG8J22_01645
LLAOLHL71_02325
LBRLVIS_1500
LBKLVISKB_1449
LKOABN16_11035
LZYLZ3411_0911
LSUAH3M12_07790
LJIELX58_06090
LSLLSL_0017
LSIHN6_00013
LSJLSJ_0020
LACALAC1533_2084
LANIFAX13_00230
LMURCPS94_09050
LBTAYR52_00460
LCYLC20004_04495
LNNF0161_11005
LKUAPS55_07070
ABOMD7I45_04080
LPDAYR62_07300
LMAEFGL90_00390
PPEPEPE_0661
PPENT256_03525
PCEPECL_1167(yxbA)
PDMADU72_1896
PACIA4V11_10130
PIOPI20285_03235
LROSLROSL1_0933
LGNABM34_06990
LHIJP39_10020
LCTBI355_1893
LALWBTM29_04605
LALILA20249_09830
LFMLF20184_01890
LZHD1B17_10870
LFTFG051_01500
CPABG6534_00420
LSALCA_0098
LCVFBA2_01665
LGMLG542_07715
LAHLA20533_03115 LA20533_07885
LBMDS830_08340
LHBD1010_13210
LDXLH506_08525 LH506_08535
WEIEQG49_04115 EQG49_09455
EFCEFAU004_01690
EFAUEFAU085_01713
EFUHMPREF0351_11643(carB2)
EFMM7W_1232
EFTM395_05775
EHREHR_10605
ECASECBG_00820
EMUEMQU_1641(carB2)
EDULIU_09540
EGAAL523_14400
ETHCK496_05070
EGVEGCR1_06315
EAVEH197_16290
ESGEsVE80_13510(carB2)
ELACI4Q40_05425
ECECNCTC12421_00981
ERAFJ9537_08570
EIEENLAB_02600
THLTEH_12780
TEYGLW17_00360
TOOC7K38_08910
TKRC7K43_02575
VTEBHY08_03300
VPIBW732_06710
VAOFA707_05560
VAHG7081_05760
VCPH9L18_10850
VHYG7082_03770
VFVK5K99_06810
VLUM2919_01610
ASANAWM72_02745
CRNCAR_c05710
CVDLHA31_04180
MARRBKP56_02300
JEPBW721_08140
JDABW727_101893
JEHEJN90_06350
JARG7057_05660
JPOG7058_05750
DPMFNV33_00965
AOEClos_0297
FPLAA4U99_01395
FPAFPR_02520
CCTCC1_14460
CCOMI6K69_13985
SINTK5I26_07860
CSTCLOST_1593
FAAHMPREF0389_00597
ROCHF520_10160
TEMJW646_03510
TGCL0P85_04790
PBQC3V37_04125
ACAEHYG86_16420
IBUIB211_00146
LASAL9O85_14670
FMSM1R53_00960
AMIJEQM06_07545
AMICAmi3637_13535
ABUTAmi103574_10645
NTHNther_2881
AVGI6H45_05550 I6H45_08405
AOBI6H46_02470 I6H46_08855
AMEILV469_06570
PMICNW74_01810
PEDING2D1G_0040
PHARNCTC13077_00771
PIVNCTC13079_00037 NCTC13079_00524
MHAPJFY71_08255
ECOUM1R54_05490
AARGAargi30884_14020(yxbA)
ABSIA9CBEGH2_14630(yxbA)
LCGL3BBH23_04820(yxbA)
MSARMSAR_28050
MANYMANY_20900
SVLStrvi_0793
SRCM271_22040
SMALSMALA_4378
SBHSBI_06116
SCXAS200_20865
SRWTUE45_05135
SCADDN051_17270
SAUOBV401_25730
SPUNBFF78_17960
SALFSMD44_05145
SALJSMD11_3779
SFKKY5_4723
SGEDWG14_03541
SLKSLUN_02465
SGDELQ87_16320
SNQCP978_19640
SKACP970_17810
SFYGFH48_17860
SAQUEJC51_28645
SRIMCP984_21920
SPHVF9278_30135
SGXH4W23_17600
SSPBCP982_24815
KITCFP65_6464
LXLKDY119_01945
PSICJ4E96_13085
AMINAUMI_17200
AUWAURUGA1_00659
ARLAFL94_15125
ARYATC04_02815
GARAOZ07_15455
GCRGcLGCM259_2601
GLUF0M17_14750
GPRJQN66_15175
NAEBHE16_08035
AULDCC27_000305
PAEYKUF55_17495
BFABfae_12240
BRXBH708_00515 BH708_00525
BRVCFK39_14070 CFK39_14080
BGGCFK41_10820 CFK41_10825
BRZCFK38_13405 CFK38_13410
BSAUDWV08_03385 DWV08_03390
BRRC1N80_12840 C1N80_12845
DVADAD186_00630 DAD186_08110
DJJCOP05_00035 COP05_06875 COP05_10850
DAYFV141_09515
LMOIVV02_09855
YIMJ5M86_04305
ALXLVQ62_16995
XCEXcel_1887 Xcel_1888
XYLET495_05720
XYAET471_15245 ET471_15250
IVAIsova_1754 Isova_1755
IDOI598_0464 I598_0465
CETB8281_13815
CCEUCBR64_17630
SKESked_16420
SANWG7063_08365
CFLCfla_1730
CFICelf_2121
CGACelgi_1490
CEZCBP52_05665
CELZE5225_09610
CEJGC089_09945
CELHGXP71_07430
CIRALFM56_09660
OEKFFI11_014330
PSEIGCE65_07120
PHWG7075_19060
PEIH9L10_00730
SERJSGUI_1445
SERWFY030_01830
ORNDV701_09085
ORZFNH13_03445
GEZFE251_08590
GYUFE374_08645
HALTIM660_10150
RHALLQF10_09825
RSUALQF12_08630
DEMLGT36_012105
SENSACE_3919
SACGFDZ84_36300
PDXPsed_1873
ATLAthai_17740
CAICaci_6401
AHEArch_0165
ARCAHC352_00790
APHOJTE88_00780
TPYCQ11_07645
TPYOX956_00020
TBWNCTC13354_01302
TPECHLG82_08485
ASGFB03_01015
ACTTDDD63_07190
AMYADJ76_02605
SOOFBF35_05935
STUCG5S47_04265
AOSAXE84_10535
ARDAXF14_10095
ACTPB6G06_04060
ACTCCHIBA101_1372
ACTOC3V41_09295
ANEAN27039_2527
AHWNCTC11636_00253 NCTC11636_00260
ACTZCWT12_06080
AIRNCTC12972_00748
ASLANCTC11923_02022 NCTC11923_02029
AVCNCTC10951_00661
AWEJG540_04820
AMAMHPC72_05665
AREPID810_05795
AVUBK816_04640
FSLEJO69_08730
FLHEJ997_10045
WIKH8R18_04075 H8R18_04515
FVGI6G91_05215
BADBAD_1086
BADLBADO_1137
BADOBBMN23_1231
BDEBDP_1523
BDNBBDE_1447
BBPBBPR_0578
BBIBBIF_0602
BBFBBB_0558
BCORBCOR_0477
BIIBINDI_0416
BANGBBAG_0555
BACTAB656_03070
BGXESN35_03540
BSUEBS3272_03765
GVAHMPREF0424_0466
SIJSCIP_0865
PDOPSDT_0275
AEYCDG81_08990
EGDGS424_012035
AEQAEQU_1453
AHATADCFC_09140
CBACJI75_06365
RTSCE91St31_21090(carB2)
OLSOlsu_1290
OLOADJ70_10590
PCATPcatena_03970(carB2)
TIOINP52_03505
CGOCorgl_0724
CAERCSV91_01335
CSTCLK434_00880
STISthe_1149
PSUBPelsub_P1647 Pelsub_P2267
KBSEPA93_47750
DDRDeide_3p00360
DMRDeima_0372
DPDDeipe_4306
RULUC8_50330
BVOPan97_27900
FMRFuma_05600
EDAGWR55_10950
SUSAcid_4867
BHLBache_1945
BISDXK01_018785
PDIBDI_0029 BDI_0559
PARCCI960_03245 CI960_05015
PRUPRU_1532
ALQC7Y71_010520
TTZFHG85_00475
AHZAPS56_16620
SYISB49_02360
AALGAREALGSMS7_04583
MARFCJ739_38
CPHCpha266_2618
IALIALB_2457
CACICLOAM0672
MVCMSVAZ_0035
MEKMSKOL_0019
METMMSMTP_0153
MHORMSHOH_0166
MELOJ7W08_11310
HDLHALDL1_03495
HHBHhub_1295
HJEHacjB3_12270
NGENatgr_3708
HLCCHINAEXTREME11135
 » show all
Reference
1  [PMID:4262567]
  Authors
Staudenbauer W, Strominger JL.
  Title
Activation of D-aspartic acid for incorporation into peptidoglycan.
  Journal
J Biol Chem 247:5095-102 (1972)
Reference
2  [PMID:4626717]
  Authors
Staudenbauer W, Willoughby E, Strominger JL.
  Title
Further studies of the D-aspartic acid-activating enzyme of Streptococcus faecalis and its attachment to the membrane.
  Journal
J Biol Chem 247:5289-96 (1972)
Reference
3  [PMID:9416615]
  Authors
Galperin MY, Koonin EV.
  Title
A diverse superfamily of enzymes with ATP-dependent carboxylate-amine/thiol ligase activity.
  Journal
Protein Sci 6:2639-43 (1997)
DOI:10.1002/pro.5560061218
Reference
4  [PMID:16510449]
  Authors
Bellais S, Arthur M, Dubost L, Hugonnet JE, Gutmann L, van Heijenoort J, Legrand R, Brouard JP, Rice L, Mainardi JL
  Title
Aslfm, the D-aspartate ligase responsible for the addition of D-aspartic acid onto the peptidoglycan precursor of Enterococcus faecium.
  Journal
J Biol Chem 281:11586-94 (2006)
DOI:10.1074/jbc.M600114200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.3.1.12
IUBMB Enzyme Nomenclature: 6.3.1.12
ExPASy - ENZYME nomenclature database: 6.3.1.12
BRENDA, the Enzyme Database: 6.3.1.12

DBGET integrated database retrieval system