KEGG   ENZYME: 6.3.1.15Help
Entry
EC 6.3.1.15                 Enzyme                                 

Name
8-demethylnovobiocic acid synthase;
novL (gene name);
novobiocin ligase;
novobiocic acid synthetase (misleading);
8-desmethyl-novobiocic acid synthetase;
8-demethylnovobiocic acid synthetase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)
BRITE hierarchy
Sysname
3-dimethylallyl-4-hydroxybenzoate:3-amino-4,7-dihydroxycoumarin ligase (AMP-forming)
Reaction(IUBMB)
ATP + 3-dimethylallyl-4-hydroxybenzoate + 3-amino-4,7-dihydroxycoumarin = AMP + diphosphate + 8-demethylnovobiocic acid [RN:R10453]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
3-dimethylallyl-4-hydroxybenzoate [CPD:C12458];
3-amino-4,7-dihydroxycoumarin [CPD:C12468]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
8-demethylnovobiocic acid [CPD:C20629]
Comment
The enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin.
History
EC 6.3.1.15 created 2013
Pathway
ec00401  Novobiocin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K12709  8-demethylnovobiocic acid synthase
Genes
NGV: CDO52_02200
Taxonomy
Reference
1  [PMID:10801869]
  Authors
Steffensky M, Li SM, Heide L
  Title
Cloning, overexpression, and purification of novobiocic acid synthetase from Streptomyces spheroides NCIMB 11891.
  Journal
J Biol Chem 275:21754-60 (2000)
DOI:10.1074/jbc.M003066200
  Sequence
Reference
2  [PMID:15218104]
  Authors
Pi N, Meyers CL, Pacholec M, Walsh CT, Leary JA
  Title
Mass spectrometric characterization of a three-enzyme tandem reaction for assembly and modification of the novobiocin skeleton.
  Journal
Proc Natl Acad Sci U S A 101:10036-41 (2004)
DOI:10.1073/pnas.0403526101
  Sequence
Reference
3  [PMID:16274243]
  Authors
Pacholec M, Tao J, Walsh CT
  Title
CouO and NovO: C-methyltransferases for tailoring the aminocoumarin scaffold in coumermycin and novobiocin antibiotic biosynthesis.
  Journal
Biochemistry 44:14969-76 (2005)
DOI:10.1021/bi051599o
Other DBs
ExplorEnz - The Enzyme Database: 6.3.1.15
IUBMB Enzyme Nomenclature: 6.3.1.15
ExPASy - ENZYME nomenclature database: 6.3.1.15
BRENDA, the Enzyme Database: 6.3.1.15

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