KEGG   ENZYME: 6.3.1.19Help
Entry
EC 6.3.1.19                 Enzyme                                 

Name
prokaryotic ubiquitin-like protein ligase;
PafA (ambiguous);
Pup ligase;
proteasome accessory factor A
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)
BRITE hierarchy
Sysname
[prokaryotic ubiquitin-like protein]:[protein]-L-lysine
Reaction(IUBMB)
ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine = ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine [RN:R11207]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
[prokaryotic ubiquitin-like protein]-L-glutamate [CPD:C21177];
[protein]-L-lysine [CPD:C02188]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine [CPD:C21178]
Comment
The enzyme has been characterized from the bacteria Mycobacterium tuberculosis and Corynebacterium glutamicum. It catalyses the ligation of the prokaryotic ubiquitin-like protein (Pup) to a target protein by forming a bond between an epsilon-amino group of a lysine residue of the target protein and the gamma-carboxylate of the C-terminal glutamate of the ubiquitin-like protein (Pup). The attachment of Pup, also known as Pupylation, marks proteins for proteasomal degradation.
History
EC 6.3.1.19 created 2015
Orthology
K13571  proteasome accessory factor A
Genes
MTU: Rv2097c(pafA)
MTV: RVBD_2097c
MTC: MT2158
MRA: MRA_2112
MTF: TBFG_12132
MTB: TBMG_01885
MTK: TBSG_01897
MTZ: TBXG_001869
MTG: MRGA327_12905
MTE: CCDC5079_1939
MTUR: CFBS_2220
MTD: UDA_2097c
MTUC: J113_14520
MTUE: J114_11240
MTUL: TBHG_02055
MTUT: HKBT1_2214
MTUU: HKBT2_2215
MBO: BQ2027_MB2124C(pafa)
MBB: BCG_2117c
MBT: JTY_2111
MBM: BCGMEX_2101c(pafA)
MBX: BCGT_1919
MAF: MAF_21110
MMIC: RN08_2325
MLE: ML1328
MLB: MLBr01328
MPA: MAP_1833c
MAO: MAP4_1995
MAVI: RC58_09955
MAVU: RE97_09965
MAV: MAV_2406
MAVD: NF84_10840
MAVR: LA63_11070
MAVA: LA64_11105
MIT: OCO_24090
MIA: OCU_23970
MID: MIP_03326
MYO: OEM_22520
MIR: OCQ_22620
MMAL: CKJ54_10915(pafA)
MUL: MUL_2329
MMC: Mmcs_2467
MKM: Mkms_2512
MJL: Mjls_2504
MMI: MMAR_3083
MMM: W7S_11615
MLI: MULP_02722(pafA1)
MHAD: B586_10675
MSG: MSMEI_3800(pafA)
MVA: Mvan_3445
MGI: Mflv_3089
MPHL: MPHLCCUG_02715(pafA)
MVQ: MYVA_3356
MAB: MAB_2183
MABB: MASS_2110
MABO: NF82_10905
MCHE: BB28_10985
MSTE: MSTE_02123(pafA)
MTER: 4434518_02041(pafA)
ASD: AS9A_2012
CGL: NCgl1437(Cgl1494)
CGB: cg1688
CGU: WA5_1437
CGT: cgR_1554
CGM: cgp_1688(pafA2)
CGJ: AR0_08005
CEF: CE1623
CDI: DIP1245
CDP: CD241_1175(pafA2)
CDH: CDB402_1154(pafA2)
CDT: CDHC01_1174(pafA2)
CDE: CDHC02_1153(pafA2)
CDR: CDHC03_1148(pafA2)
CDA: CDHC04_1158(pafA2)
CDZ: CD31A_1256(pafA2)
CDB: CDBH8_1223(pafA2)
CDS: CDC7B_1239(pafA2)
CDD: CDCE8392_1146(pafA2)
CDW: CDPW8_1224(pafA2)
CDV: CDVA01_1115(pafA2)
CDIP: ERS451417_01245(pafA2)
CJK: jk0945
CUR: cu1043
CUA: CU7111_1025(pafA2)
CKP: ckrop_0910(pafA2)
CPU: cpfrc_01037(pafA2)
CPL: Cp3995_1056(pafA)
CPG: Cp316_1077(pafA)
CPP: CpP54B96_1052(pafA)
CPK: Cp1002_1033(pafA)
CPQ: CpC231_1032(pafA)
CPX: CpI19_1038(pafA)
CPZ: CpPAT10_1032(pafA)
COR: Cp267_1081(pafA)
COP: Cp31_1042(pafA)
COD: Cp106_1015(pafA)
COS: Cp4202_1025(pafA)
COI: CpCIP5297_1050(pafA)
COE: Cp258_1048(pafA)
COU: Cp162_1031(pafA)
CPSE: CPTA_01604
CPSU: CPTB_02159
CPSF: CPTC_01595
CRD: CRES_1023(pafA2)
CUC: CULC809_01093(pafA2)
CUN: Cul210932_1149(pafA)
CUS: CulFRC11_1117(pafA)
CUQ: Cul210931_1099(pafA)
CUZ: Cul05146_1173(pafA)
CUJ: CUL131002_1131c(pafA)
CVA: CVAR_1421
CTER: A606_05520
CGY: CGLY_08740(pafA)
COA: DR71_64(pafA)
CSX: CSING_06960(pafA)
CMQ: B840_06270(pafA)
CKU: UL82_05180(pafA)
CCJ: UL81_05990(pafA)
CMV: CMUST_08010(pafA)
CEI: CEPID_06380(pafA)
CTED: CTEST_06610(pafA)
CUT: CUTER_05430(pafA)
CDX: CDES_07215(pafA)
CSP: WM42_0091(pafA)
CPHO: CPHO_06095
CGV: CGLAU_06375(pafA)
CAQU: CAQU_06335
CAMG: CAMM_06720
NFA: NFA_31700
NFR: ERS450000_00902(pafA)
NCY: NOCYR_3335(pafA)
NNO: NONO_c34100(pafA)
NSR: NS506_05082(pafA)
NTP: CRH09_17345(pafA)
RER: RER_31710(pafA)
REY: O5Y_14565
ROP: ROP_05790(pafA)
REQ: REQ_23310
RHB: NY08_1006
RFA: A3L23_04257(pafA)
RHS: A3Q41_04009(pafA)
RRZ: CS378_03515(pafA)
RHU: A3Q40_01727(pafA)
RQI: C1M55_15805(pafA)
GBR: Gbro_2465
GPO: GPOL_c23990(pafA)
GOR: KTR9_2374
GOC: CXX93_12500(pafA)
GIT: C6V83_11435(pafA)
TPR: Tpau_2166
SRT: Srot_1603
DIT: C3V38_13700(pafA)
DIZ: CT688_07875(pafA)
SCO: SCO1640(SCI41.23c)
SGR: SGR_5863
SGB: WQO_05965
SCT: SCAT_5177
SFA: Sfla_5196
SBH: SBI_08486
SHY: SHJG_3077
SVE: SVEN_1233
SDV: BN159_6943(pafA)
SALB: XNR_5187
SALS: SLNWT_6263
STRP: F750_1438(pafA)
SFI: SFUL_1158
SALU: DC74_2110
SALL: SAZ_11115
SLV: SLIV_29540(pafA)
SGU: SGLAU_06775(pafA)
STRE: GZL_07028
SLD: T261_6604
STRM: M444_08535
SAMB: SAM23877_1695(pafA)
SPRI: SPRI_5915
SRW: TUE45_02066(pafA)
SLE: sle_54770(sle_54770)
SRN: A4G23_00873(pafA)
SNR: SNOUR_32210(pafA)
STRD: NI25_30990
SMAL: SMALA_1428
SLAU: SLA_1175
SALF: SMD44_01695(pafA)
SALJ: SMD11_5383(pafA)
SLX: SLAV_29310(pafA)
SFK: KY5_1442c
KSK: KSE_66650(pafA)
ART: Arth_2178
ARM: ART_0666
ARN: CGK93_12645(pafA)
ARX: ARZXY2_1801(pafA)
ARTH: C3B78_10245(pafA)
AAU: AAur_2179
ACH: Achl_1921
KRH: KRH_13850
RDN: HMPREF0733_11951(pafA)
BCV: Bcav_2237
BRV: CFK39_00595(pafA)
BGG: CFK41_08660(pafA)
BRZ: CFK38_10865(pafA)
JDE: Jden_1206
LMOI: VV02_15885
XCE: Xcel_1490
IDO: I598_0768(pafA)
CFL: Cfla_1999
CFI: Celf_1794
SERJ: SGUI_0335
BLIN: BLSMQ_2079
DCO: SAMEA4475696_1703(pafA)
MPH: MLP_17560(pafA)
NCA: Noca_2648
NDK: I601_3312(pafA)
KFL: Kfla_5110
PSIM: KR76_13420
AEZ: C3E78_09335(pafA)
MGG: MPLG2_1733(pafA)
TFU: Tfu_1788
NDA: Ndas_1497
NAL: B005_4102(pafA)
NGV: CDO52_13730(pafA)
TCU: Tcur_2313
SRO: Sros_5868
FAL: FRAAL2878
ACE: Acel_1191
NML: Namu_2960
GOB: Gobs_2684
BSD: BLASA_2671(pafA)
MMAR: MODMU_2605(pafA)
KRA: Krad_1875
SEN: SACE_2254
AMD: AMED_4890
AMN: RAM_24890
AMM: AMES_4831
AMZ: B737_4831
AOI: AORI_3826(pafA)
AJA: AJAP_20030(pafA)
AMYC: CU254_18280(pafA)
PDX: Psed_3293
PSEA: WY02_02410
PSEE: FRP1_12100
PSEH: XF36_10485
AMI: Amir_2247
APRE: CNX65_11435(pafA)
SESP: BN6_31900(pafA)
KAL: KALB_3053
ACTI: UA75_12985
ACAD: UA74_12905
AHG: AHOG_12190(pafA)
ACTA: C1701_22595(pafA)
ALL: CRK56432
SAQ: Sare_2364
MIL: ML5_5437
ASE: ACPL_5241
ACTN: L083_5066
AFS: AFR_26390
ACTS: ACWT_5111
PLK: CIK06_14035(pafA)
PLAB: C6361_03190(pafA)
PLAT: C6W10_18445(pafA)
CAI: Caci_2428
SNA: Snas_4459
AHE: Arch_0902
TPYO: X956_05435
ACTT: DDD63_05840(pafA)
ACTO: C3V41_03825(pafA)
BLO: BL1797
BLJ: BLD_1975
BLN: Blon_2124
BLON: BLIJ_2201
BLF: BLIF_1510
BLL: BLJ_1494
BLB: BBMN68_26
BLM: BLLJ_1465
BLG: BIL_01800
BAD: BAD_0549
BADL: BADO_0563
BLA: BLA_1169
BBB: BIF_01344
BBC: BLC1_0614
BLV: BalV_0618
BLW: W7Y_0645(pafA)
BLS: W91_0666
BANI: Bl12_0598
BANL: BLAC_03225
BANM: EN10_03265
BDE: BDP_0755
BDN: BBDE_0723
BBI: BBIF_0658(pafA)
BBP: BBPR_0634
BBF: BBB_0618
BBV: HMPREF9228_0491(pafA)
BBRU: Bbr_1380
BBRE: B12L_1321
BBRV: B689b_1403
BBRJ: B7017_1582
BBRC: B7019_1568
BBRN: B2258_1355
BBRS: BS27_1398
BBRD: BBBR_1436
BAST: BAST_1106
BTP: D805_1148
BCOR: BCOR_0941
BKS: BBKW_0615
BPSP: AH67_03540
BANG: BBAG_0455
BCAT: BBCT_0552
BPSC: BBPC_0603
BSCA: BBSC_0980
PDO: PSDT_1158
TBI: Tbis_1829
AEY: CDG81_14130(pafA)
AFO: Afer_0912
AYM: YM304_26020(pafA)
MIN: Minf_1278
NJA: NSJP_0043(pafA) NSJP_1985
LFC: LFE_0660
MOX: DAMO_0753
 » show all
Taxonomy
Reference
1  [PMID:20355727]
  Authors
Sutter M, Damberger FF, Imkamp F, Allain FH, Weber-Ban E
  Title
Prokaryotic ubiquitin-like protein (Pup) is coupled to substrates via the side chain of its C-terminal glutamate.
  Journal
J Am Chem Soc 132:5610-2 (2010)
DOI:10.1021/ja910546x
Reference
2  [PMID:21081505]
  Authors
Guth E, Thommen M, Weber-Ban E
  Title
Mycobacterial ubiquitin-like protein ligase PafA follows a two-step reaction pathway with a phosphorylated pup intermediate.
  Journal
J Biol Chem 286:4412-9 (2011)
DOI:10.1074/jbc.M110.189282
Reference
3  [PMID:23471967]
  Authors
Ofer N, Forer N, Korman M, Vishkautzan M, Khalaila I, Gur E
  Title
Allosteric transitions direct protein tagging by PafA, the prokaryotic ubiquitin-like protein (Pup) ligase.
  Journal
J Biol Chem 288:11287-93 (2013)
DOI:10.1074/jbc.M112.435842
Reference
4  [PMID:23601177]
  Authors
Barandun J, Delley CL, Ban N, Weber-Ban E
  Title
Crystal structure of the complex between prokaryotic ubiquitin-like protein and its ligase PafA.
  Journal
J Am Chem Soc 135:6794-7 (2013)
DOI:10.1021/ja4024012
Reference
5  [PMID:23557784]
  Authors
Striebel F, Imkamp F, Ozcelik D, Weber-Ban E
  Title
Pupylation as a signal for proteasomal degradation in bacteria.
  Journal
Biochim Biophys Acta 1843:103-13 (2014)
DOI:10.1016/j.bbamcr.2013.03.022
Other DBs
ExplorEnz - The Enzyme Database: 6.3.1.19
IUBMB Enzyme Nomenclature: 6.3.1.19
ExPASy - ENZYME nomenclature database: 6.3.1.19
BRENDA, the Enzyme Database: 6.3.1.19

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