| Entry |
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| Name |
enterobactin synthase;
N-(2,3-dihydroxybenzoyl)-serine synthetase;
2,3-dihydroxybenzoylserine synthetase;
2,3-dihydroxybenzoate---serine ligase
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| Class |
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
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| Sysname |
2,3-dihydroxybenzoate:L-serine ligase
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| Reaction(IUBMB) |
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine = enterobactin + 6 AMP + 6 diphosphate [RN: R07644]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
This enzyme complex catalyses the conversion of three molecules each of 2,3-dihydroxybenzoate and L-serine to form the siderophore enterobactin. In Escherichia coli the complex is formed by EntB (an aryl carrier protein that has to be activated by 4'-phosphopantetheine), EntD (a phosphopantetheinyl transferase that activates EntB), EntE (catalyses the ATP-dependent condensation of 2,3-dihydroxybenzoate and holo-EntB to form the covalently arylated form of EntB), and EntF (a four domain protein that catalyses the activation of L-serine by ATP, the condensation of the activated L-serine with the activated 2,3-dihydroxybenzoate, and the trimerization of three such moieties to a single enterobactin molecule).
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| History |
EC 6.3.2.14 created 1972, modified 2012
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| Pathway |
| ec01053 | Biosynthesis of siderophore group nonribosomal peptides |
| ec01110 | Biosynthesis of secondary metabolites |
| ec01130 | Biosynthesis of antibiotics |
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| Orthology |
| K01252 | bifunctional isochorismate lyase / aryl carrier protein |
| K02362 | enterobactin synthetase component D |
| K02363 | 2,3-dihydroxybenzoate-AMP ligase |
| K02364 | enterobactin synthetase component F |
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| Genes |
» show all
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| Reference |
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| Authors |
Brot N, Goodwin J. |
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| Title |
Regulation of 2,3-dihydroxybenzoylserine synthetase by iron. |
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| Journal |
J Biol Chem 243:510-3 (1968) |
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| Reference |
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| Authors |
Rusnak F, Faraci WS, Walsh CT. |
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| Title |
Subcloning, expression, and purification of the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase: demonstration of enzyme-bound (2,3-dihydroxybenzoyl)adenylate product. |
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| Journal |
Biochemistry 28:6827-35 (1989) |
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| Reference |
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| Authors |
Rusnak F, Liu J, Quinn N, Berchtold GA, Walsh CT |
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| Title |
Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase. |
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| Journal |
Biochemistry 29:1425-35 (1990) |
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| Sequence |
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| Reference |
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| Authors |
Rusnak F, Sakaitani M, Drueckhammer D, Reichert J, Walsh CT |
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| Title |
Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine. |
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| Journal |
Biochemistry 30:2916-27 (1991) |
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| Sequence |
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| Reference |
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| Authors |
Gehring AM, Mori I, Walsh CT. |
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| Title |
Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF. |
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| Journal |
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| Reference |
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| Authors |
Shaw-Reid CA, Kelleher NL, Losey HC, Gehring AM, Berg C, Walsh CT |
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| Title |
Assembly line enzymology by multimodular nonribosomal peptide synthetases: the thioesterase domain of E. coli EntF catalyzes both elongation and cyclolactonization. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 6.3.2.14 |
| ExPASy - ENZYME nomenclature database: | 6.3.2.14 |
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