| Entry |
|
|---|
| Name |
enterobactin synthase;
N-(2,3-dihydroxybenzoyl)-serine synthetase;
2,3-dihydroxybenzoylserine synthetase;
2,3-dihydroxybenzoate---serine ligase
|
|---|
| Class |
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
|
|---|
| Sysname |
2,3-dihydroxybenzoate:L-serine ligase
|
|---|
| Reaction(IUBMB) |
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine = enterobactin + 6 AMP + 6 diphosphate [RN: R07644]
|
|---|
| Reaction(KEGG) |
|
|---|
| Substrate |
|
|---|
| Product |
|
|---|
| Comment |
This enzyme complex catalyses the conversion of three molecules each of 2,3-dihydroxybenzoate and L-serine to form the siderophore enterobactin. In Escherichia coli the complex is formed by EntB (an aryl carrier protein that has to be activated by 4'-phosphopantetheine), EntD (a phosphopantetheinyl transferase that activates EntB), EntE (catalyses the ATP-dependent condensation of 2,3-dihydroxybenzoate and holo-EntB to form the covalently arylated form of EntB), and EntF (a four domain protein that catalyses the activation of L-serine by ATP, the condensation of the activated L-serine with the activated 2,3-dihydroxybenzoate, and the trimerization of three such moieties to a single enterobactin molecule).
|
|---|
| History |
EC 6.3.2.14 created 1972, modified 2012
|
|---|
| Pathway |
| ec01053 | Biosynthesis of siderophore group nonribosomal peptides |
| ec01110 | Biosynthesis of secondary metabolites |
|
|---|
| Orthology |
| K01252 | bifunctional isochorismate lyase / aryl carrier protein |
| K02362 | enterobactin synthetase component D |
| K02363 | 2,3-dihydroxybenzoate-AMP ligase |
| K02364 | enterobactin synthetase component F |
|
|---|
| Genes |
» show all
|
|---|
| Reference |
|
|---|
| Authors |
Brot N, Goodwin J. |
|---|
| Title |
Regulation of 2,3-dihydroxybenzoylserine synthetase by iron. |
|---|
| Journal |
J Biol Chem 243:510-3 (1968) |
|---|
| Reference |
|
|---|
| Authors |
Rusnak F, Faraci WS, Walsh CT. |
|---|
| Title |
Subcloning, expression, and purification of the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase: demonstration of enzyme-bound (2,3-dihydroxybenzoyl)adenylate product. |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
Rusnak F, Liu J, Quinn N, Berchtold GA, Walsh CT |
|---|
| Title |
Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase. |
|---|
| Journal |
|
|---|
| Sequence |
|
|---|
| Reference |
|
|---|
| Authors |
Rusnak F, Sakaitani M, Drueckhammer D, Reichert J, Walsh CT |
|---|
| Title |
Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine. |
|---|
| Journal |
|
|---|
| Sequence |
|
|---|
| Reference |
|
|---|
| Authors |
Gehring AM, Mori I, Walsh CT. |
|---|
| Title |
Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF. |
|---|
| Journal |
|
|---|
| Reference |
|
|---|
| Authors |
Shaw-Reid CA, Kelleher NL, Losey HC, Gehring AM, Berg C, Walsh CT |
|---|
| Title |
Assembly line enzymology by multimodular nonribosomal peptide synthetases: the thioesterase domain of E. coli EntF catalyzes both elongation and cyclolactonization. |
|---|
| Journal |
|
|---|
| Sequence |
|
|---|
| Other DBs |
| ExplorEnz - The Enzyme Database: | 6.3.2.14 |
| ExPASy - ENZYME nomenclature database: | 6.3.2.14 |
|
|---|
