KEGG   ENZYME: 6.3.2.25
Entry
EC 6.3.2.25                 Enzyme                                 
Name
tubulin---tyrosine ligase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
alpha-tubulin:L-tyrosine ligase (ADP-forming)
Reaction(IUBMB)
ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate [RN:R04730]
Reaction(KEGG)
R04730
Substrate
ATP [CPD:C00002];
detyrosinated alpha-tubulin [CPD:C05234];
L-tyrosine [CPD:C00082]
Product
alpha-tubulin [CPD:C01392];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
L-Tyrosine is linked via a peptide bond to the C-terminus of de-tyrosinated alpha-tubulin (des-Tyromega-alpha-tubulin). The enzyme is highly specific for alpha-tubulin and moderately specific for ATP and L-tyrosine. L-Phenylalanine and 3,4-dihydroxy-L-phenylalanine are transferred but with higher Km values.
History
EC 6.3.2.25 created 1999
Orthology
K06047  tubulin---tyrosine ligase
Genes
HSA150465(TTL)
PTR470483(TTL)
PPS100969421(TTL)
GGO101139501 115932650
PON100450789(TTL)
NLE100599199(TTL)
HMH116482958(TTL)
MCC699075(TTL)
MCF102146185(TTL)
MTHB126933409
MNI105471795(TTL)
CSAB103241269(TTL)
CATY105591134(TTL)
PANU101018858(TTL)
TGE112605186(TTL)
MLEU105530461(TTL)
RRO104680394(TTL)
RBB108518062(TTL)
TFN117062195(TTL)
PTEH111528226(TTL)
CANG105519659(TTL)
CJC100404587(TTL)
SBQ101050847(TTL) 104652891
CIMI108318639(TTL)
CSYR103259446(TTL)
MMUR105859196(TTL)
LCAT123637174(TTL)
PCOQ105820316(TTL)
OGA100946136(TTL)
MMU69737(Ttl)
MCAL110285528(Ttl) 110295428
MPAH110318736(Ttl)
RNO171572(Ttl)
MCOC116090909(Ttl)
ANU117704063(Ttl)
MUN110551397(Ttl)
CGE100755055(Ttl)
MAUA101838038(Ttl)
PROB127221460(Ttl)
PLEU114709512(Ttl)
MORG121460423(Ttl)
MFOT126496701
AAMP119815711(Ttl)
NGI103739890(Ttl)
HGL101701462(Ttl)
CPOC100723990(Ttl)
CCAN109692039 109692049
DORD105988162(Ttl)
DSP122121366(Ttl)
PLOP125356080(Ttl)
NCAR124963043
MMMA107154158(Ttl)
OCU100353592
OPI101529711(TTL)
TUP102487597(TTL)
GVR103608255(TTL)
CFA483065(TTL)
CLUD112664398(TTL)
VVP112916643(TTL)
VLG121491255(TTL)
NPO129511002(TTL)
AML100474988(TTL)
UMR103671867(TTL)
UAH113242468(TTL)
UAR123799752(TTL)
ELK111158497
LLV125109051
MPUF101690525(TTL)
MNP132021355(TTL)
MLK131840435(TTL)
NVS122915089(TTL)
ORO101380303(TTL)
EJU114223495(TTL)
ZCA113937252(TTL)
MLX118007857(TTL)
NSU110587360(TTL)
LWW102741193(TTL)
FCA102900797(TTL)
PYU121044450(TTL)
PCOO112864159(TTL)
PBG122497177(TTL)
LRUF124503787
PTG102966737(TTL)
PPAD109264639(TTL)
PUC125937440
AJU106976025
HHV120231593(TTL)
BTA514559(TTL)
BOM102280366(TTL)
BIU109566127(TTL)
BBUB102411049(TTL)
BBIS104984419(TTL)
CHX102180860(TTL)
OAS101107272(TTL)
BTAX128055418(TTL)
ODA120851451(TTL)
CCAD122441790(TTL)
MBEZ129539499(TTL)
SSC445530(TTL)
CFR102509422(TTL)
CBAI105072143(TTL)
CDK105093106(TTL)
VPC102531122(TTL)
BACU103018295(TTL)
LVE103087944(TTL)
OOR101270645(TTL)
DLE111187444(TTL)
PCAD102983540(TTL)
PSIU116764181(TTL)
NASI112398961(TTL)
ECB100052052(TTL)
EPZ103559679(TTL)
EAI106838551(TTL)
MYB102252588(TTL)
MYD102760056(TTL)
MMYO118659622(TTL)
MLF102439493(TTL)
MNA107528635(TTL)
PKL118717644(TTL)
EFUS103303787(TTL)
HAI109393550(TTL)
DRO112303048(TTL)
SHON118981846(TTL)
AJM119042476(TTL)
PDIC114500232(TTL)
PHAS123811096(TTL)
MMF118631169(TTL)
RFQ117019332(TTL)
PPAM129072144(TTL)
PALE102891762(TTL)
PGIG120610304(TTL)
PVP105303990(TTL)
RAY107514971(TTL)
MJV108401296(TTL)
TOD119258618(TTL) 119259193
SARA101549284(TTL)
LAV100675880(TTL)
TMU101357599
ETF101663942(TTL)
DNM101443801(TTL)
MDO100031152(TTL)
GAS123236927(TTL)
SHR100930667(TTL)
AFZ127548408
PCW110209200(TTL)
OAA100084789(TTL)
GGA416711(TTL)
PCOC116231542(TTL)
MGP100540631(TTL)
CJO107310907(TTL)
TPAI128077287(TTL)
LMUT125689495(TTL)
NMEL110395293(TTL)
APLA101798438 119716451 119716471
ACYG106044757(TTL)
CATA118255239(TTL)
AFUL116487231(TTL)
TGU100218069(TTL)
LSR110472814(TTL)
SCAN103821312(TTL)
PMOA120500919(TTL)
OTC121346335(TTL)
PRUF121353913(TTL)
GFR102044350(TTL)
FAB101817591(TTL)
OMA130251194(TTL)
PHI102110480(TTL)
PMAJ107202369(TTL)
CCAE111926609(TTL)
CCW104696672
CBRC103617479
ETL114066754(TTL)
ZAB102075238(TTL)
ACHL103800018(TTL)
SVG106862725(TTL)
MMEA130581304(TTL)
HRT120751303(TTL)
FPG101921873(TTL)
FCH102046034(TTL)
CLV102097117(TTL)
EGZ104128802(TTL)
NNI104015810(TTL)
PCRI104035617(TTL)
PLET104620659(TTL)
EHS104504369(TTL)
PCAO104047299(TTL)
ACUN113477983(TTL)
TALA104359847(TTL)
PADL103923112(TTL)
AFOR103893484(TTL)
ACHC115350084(TTL)
HALD104316989(TTL)
HLE104838193(TTL)
AGEN126052630
GCL127014864
CCRI104159920(TTL)
CSTI104558836(TTL)
CMAC104485518(TTL)
MUI104536267(TTL)
BREG104631003(TTL)
FGA104078288(TTL)
GSTE104264671(TTL)
LDI104352939
MNB103774548(TTL)
OHA104334336(TTL)
NNT104402897(TTL)
SHAB115613720(TTL)
DPUB104300925(TTL)
PGUU104459553(TTL)
ACAR104532412
CPEA104395325(TTL)
AVIT104268840(TTL)
CVF104295155(TTL)
RTD128906921(TTL)
CUCA104060748(TTL)
TEO104374353(TTL)
BRHI104488338(TTL)
AAM106494133(TTL)
AROW112973555(TTL)
NPD112949347(TTL)
TGT104570054(TTL)
DNE112985512(TTL)
SCAM104142964(TTL)
ASN102387034(TTL)
AMJ102565195(TTL)
CPOO109319423(TTL)
GGN109304464(TTL)
PSS102459357(TTL)
CMY102931299(TTL)
CCAY125633264(TTL)
DCC119853140(TTL)
CPIC101933325(TTL)
TST117874129(TTL)
CABI116819117(TTL)
MRV120401121(TTL)
ACS100561131(ttl)
ASAO132761527(TTL)
PVT110087024(TTL)
SUND121915894(TTL)
PBI103067995(TTL)
PMUR107296307(TTL)
CTIG120299581(TTL)
TSR106537996(TTL)
PGUT117671584(TTL)
APRI131189175(TTL)
PTEX113433366(TTL)
NSS113422962(TTL)
VKO123022814(TTL)
PMUA114594790(TTL)
PRAF128411260(TTL)
ZVI118083069(TTL)
HCG128352891(TTL)
GJA107114599(TTL)
STOW125424842(TTL)
EMC129334903(TTL)
XLA108716622(ttl.L)
XTR100135000(ttl)
NPR108801118(TTL)
RTEM120937675(TTL)
BBUF120999271
BGAR122936175(TTL)
MUO115466693(TTL)
GSH117356821(TTL)
DRE767687(ttl)
SRX107708435 107721225
SANH107678537 107698152
SGH107577318 107582496(ttl)
CCAR109068870 109094090
CAUA113057189 113112963
CGIB127970537 128026427
PTET122356673(ttl)
LROH127174692(ttl)
OMC131552718(ttl)
PPRM120479576(ttl)
RKG130083135(ttl)
MAMB125276654(ttl)
CIDE127525428
MASI127412101 127413848
TROS130559622(ttl)
TDW130432185(ttl)
MANU129415990(ttl)
IPU108263154
IFU128605562(ttl)
PHYP113545255(ttl)
SMEO124377520(ttl)
TFD113639846(ttl)
TVC132847255(ttl)
AMEX103045419(ttl)
CMAO118810355(ttl)
EEE113587313(ttl)
CHAR105910215(ttl)
TRU101075885(ttl)
TFS130533381(ttl)
TNGGSTEN00007034G001
LCO104921860(ttl)
NCC104949570(ttl)
TBEN117499837(ttl)
CGOB115019920(ttl)
PGEO117459775(ttl)
GACU117552091(ttl)
ELY117248562(ttl)
EFO125903691(ttl)
PLEP121954405(ttl)
SLUC116039423(ttl)
ECRA117960585(ttl)
ESP116675698(ttl)
PFLV114572518(ttl)
GAT120820392(ttl)
PPUG119214116(ttl)
AFB129092359(ttl)
CLUM117743884(ttl)
MSAM119917174(ttl)
SCHU122884827(ttl)
CUD121510814(ttl)
ALAT119033586(ttl)
MZE101486420(ttl)
ONL100706993(ttl)
OAU116318784(ttl)
OLA101168672(ttl)
OML112161869(ttl)
XMA102227655(ttl)
XCO114138410(ttl)
XHE116713590(ttl)
PRET103477148(ttl)
PFOR103156978(ttl)
PLAI106952677(ttl)
PMEI106921252(ttl)
GAF122842774(ttl)
CVG107088142(ttl)
CTUL119781070(ttl)
GMU124859339(ttl)
NFU107375537(ttl)
KMR108247179(ttl)
ALIM106525593(ttl)
NWH119410737(ttl)
AOCE111573098(ttl)
MCEP125018654(ttl)
CSEM103387495(ttl)
POV109633843(ttl)
SSEN122782205(ttl)
HHIP117775413(ttl)
HSP118119195(ttl)
LCF108886280
SDU111220626(ttl)
SLAL111669486(ttl)
XGL120796369(ttl)
HCQ109523162(ttl)
SSCV125978549
SBIA133510105(ttl)
PEE133409997(ttl)
PTAO133485674(ttl)
BPEC110168527(ttl)
MALB109962255(ttl)
BSPL114870658(ttl)
SJO128372781(ttl)
SASA106576805 106604770
STRU115153031 115193982
OTW112252826 112252858(ttl)
OMY110502294(ttl) 110526580
OGO124038131 124041420
ONE115113367 115135286
OKI109889794 109899605
OKE118369527 118401797
SALP111951844 111977769
SNH120017533 120046477
CCLU121553177 121569066(ttl)
ELS105029715(ttl)
SFM108925774(ttl)
PKI111849582(ttl)
AANG118217597(ttl)
LOC102695224(ttl)
PSPA121315654 121316727
ARUT117402225 117410344
PSEX120516932(ttl)
LCM102348001(TTL)
CMK103178932(ttl)
RTP109936552(ttl)
CPLA122548480(ttl)
HOC132831015(ttl)
LERI129697615(ttl)
PMRN116947807(TTL)
LRJ133341348(TTL)
BFO118420043
BBEL109471027
SPU582881
LPIC129265068
APLC110983169
AJC117108453
SKO100372948
LGILOTGIDRAFT_118802
PVUL126820913
GAE121371504
HRF124129909
HRJ124285975
CRG105332425
CVN111128710
CANU128175093
OED125649864
MYI110454341 110464299
PMAX117322895
MCAF127698609
MMER123532778
RPHI132719907
DPOL127874548
LAK106165091
NVE5514895
EPA110249149
ATEN116300315
ADF107353067
AMIL114953547
PDAM113674428
SPIS111318832
TADTRIADDRAFT_52733
VCNVOLCADRAFT_105441(ttlD)
MISMICPUN_102087
MPPMICPUCDRAFT_59804(JGI:MicpuC2_59804)
SCEYBR094W(PBY1)
SEUBDI49_1126
SPAOSPAR_B01970
AGOAGOS_ADL312C
KLAKLLA0_B12012g
KMXKLMA_70330(PBY1)
LTHKLTH0E14894g
VPOKpol_376p3
ZROZYRO0C15796g
CGRCAGL0H08503g
NCSNCAS_0I01240(NCAS0I01240)
NDINDAI_0A07760(NDAI0A07760)
TPFTPHA_0A03720(TPHA0A03720)
TBLTBLA_0B02540(TBLA0B02540)
TGBHG536_0B02440
TDLTDEL_0C04770(TDEL0C04770)
KAFKAFR_0G01640(KAFR0G01640)
KNGKNAG_0J02490(KNAG0J02490)
ZMKHG535_0A02480
PPAPAS_chr4_0137
DHADEHA2D17754g
PICPICST_74933(YBU4)
PGUPGUG_05242
SPAASPAPADRAFT_155983
LELPVL30_004065(PBY1)
CALCAALFM_C401730CA(CaO19.4621)
CTPCTRG_00209
COTCORT_0E01300
CDUCD36_41630
CTENCANTEDRAFT_127122
YLIYALI0C11495g
CLUCLUG_02124
CLUSA9F13_02g01650
CAURCJI96_0004024
SLBAWJ20_3514(PBY1) AWJ20_3515(PBY1)
PKZC5L36_0D05060
BNNFOA43_003579 FOA43_003580
BBRXBRETT_004441
OPAHPODL_04942
SLUDSCDLUD_000783
NCRNCU04743
NTENEUTE1DRAFT87071(NEUTE1DRAFT_87071)
SMPSMAC_03377
PANPODANSg174 PODANSg3000
TTTTHITE_2110204
MTMMYCTH_2306820 MYCTH_74402
CTHRCTHT_0042910 CTHT_0049290
MGRMGG_02486
PPEIPpBr36_07077
PGRIPgNI_05638
TMNUCRPA7_749
SSCKSPSK_01002
FGRFGSG_03182 FGSG_09516
FPUFPSE_01062 FPSE_05997
FPOAFPOAC1_005533 FPOAC1_012145
FVNFVRRES_06106 FVRRES_13220
FVRFVEG_08450 FVEG_15395
FOXFOXG_06212 FOXG_10053 FOXG_12440 FOXG_14097
NHENECHADRAFT_102179 NECHADRAFT_58510
FFCNCS54_00296400 NCS54_01454100
FMUJ7337_004071 J7337_012890
TRETRIREDRAFT_120402
TRRM419DRAFT_24029
MAWMAC_02712
MAJMAA_02096
PCHMVFPPC_01599
CMTCCM_04038
AMUSLMH87_009191
PLJVFPFJ_04003
PTKZJDV02_004522
VALVDBG_08145 VDBG_08146 VDBG_09123
VDAVDAG_07271 VDAG_10040
CFJCFIO01_03847 CFIO01_05151
CLUPCLUP02_18341
CHIGCH63R_13490 CH63R_14087
SAPOSAPIO_CDS0824
ELAUCREL1_1880 UCREL1_2419 UCREL1_4197
PFYPFICI_10317
SSLSS1G_06660
BFUBCIN_11g04050
MBEMBM_09677
PSCOLY89DRAFT_730531
GLZGLAREA_00155
ANIAN4967.2
AFMAFUA_3G10220
ACTACLA_038080
NFINFIA_067060
AORAO090003000557
ANGAn16g04080
AFVAFLA_001132
ALUCAKAW2_70346A
ACHEACHE_20993A
APUUAPUU_30889A
PCSN7525_003132
PDPPDIP_28850
POUPOX_b03213
TMFEYB26_000476
TRGTRUGW13939_04393
CIMCIMG_03574
CPWCPC735_006880
UREUREG_07354
PBLPAAG_01893
PBNPADG_00569
ABEARB_05464 ARB_05465
TVETRV_02379 TRV_02380
AJEHCAG_00093
BGHBDBG_06370
PNOSNOG_07971 SNOG_10458
PTEPTT_11863 PTT_16804
BZECOCCADRAFT_1329 COCCADRAFT_27323
BSCCOCSADRAFT_29737
BORCOCMIDRAFT_2314 COCMIDRAFT_24749
AALTCC77DRAFT_242281 CC77DRAFT_403761
ARABEKO05_0004986
ZTRMYCGRDRAFT_103350 MYCGRDRAFT_37793
PFJMYCFIDRAFT_201668 MYCFIDRAFT_202394
FFUCLAFUR5_10107
CBETCB0940_06258
BCOMBAUCODRAFT_273847 BAUCODRAFT_32516
NPAUCRNP2_5182 UCRNP2_5216
TMLGSTUM_00001386001
SPOSPAC12B10.04
SOMSOMG_03757(pby1)
CNECNA01340 CNL06170 CNL06540
CNBCNBA1300 CNBI0650
CNGCNAG_00146 CNAG_04997
CGICGB_B1570C CGB_D0270W CGB_D0570C
TMSTREMEDRAFT_42822 TREMEDRAFT_68211
TASAA1Q1_07249 A1Q1_07338
CCACCcaHIS019_0108720(PBY1) CcaHIS019_0204020(CcaverHIS019_0204020) CcaHIS019_0601760(PBY1)
TVSTRAVEDRAFT_68505
DSQDICSQDRAFT_123228 DICSQDRAFT_45990
SHSSTEHIDRAFT_129909 STEHIDRAFT_151781 STEHIDRAFT_90330
HIRHETIRDRAFT_307270 HETIRDRAFT_431094
PSQPUNSTDRAFT_117256
ADLAURDEDRAFT_86701
FMEFOMMEDRAFT_167210 FOMMEDRAFT_82892
GTRGLOTRDRAFT_113331 GLOTRDRAFT_118917
RSXRhiXN_00515 RhiXN_00800
LBCLACBIDRAFT_242904 LACBIDRAFT_243814
CCICC1G_03660 CC1G_06724
ABPAGABI1DRAFT110465(AGABI1DRAFT_110465) AGABI1DRAFT66676(AGABI1DRAFT_66676)
ABVAGABI2DRAFT189636(AGABI2DRAFT_189636) AGABI2DRAFT197617(AGABI2DRAFT_197617)
PCUBJR316_0000977
MRRMoror_104 Moror_13257
SCMSCHCO_02490054(SCHCODRAFT_02490054) SCHCO_02528156(SCHCODRAFT_02528156)
CPUTCONPUDRAFT_115032 CONPUDRAFT_78963
SLASERLADRAFT_444900
WSEWALSEDRAFT_65763
WICJ056_003045
UMAUMAG_03366 UMAG_05266
PFPPFL1_05398 PFL1_06524
SGRAEX895_003022 EX895_005489
MGLMGL_1845
MRTMRET_1965
PGRPGTG_18918 PGTG_21394
PTRCPtA15_3A99 PtA15_5A514
PSTRPst134EA_006827 Pst134EA_032001
MLRMELLADRAFT_102244
MBRMONBRDRAFT_14687
ACANACA1_126460
PTMGSPATT00017619001
PTIPHATRDRAFT_42815
NGDNGA_2076410 NGA_2076420
PIFPITG_05632
PSOJPHYSODRAFT_484414
SPARSPRG_02342 SPRG_07498
EHXEMIHUDRAFT_42928
GTTGUITHDRAFT_108359
TBRTb927.2.5250
TBGTbgDal_II3280
TCR507801.50
LMALMJF_27_2490
LIFLINJ_27_2440
LDOLDBPK_272440
LMILMXM_27_2490
LBZLBRM_27_2700
LPANLPMP_272520
NGRNAEGRDRAFT_78886
 » show all
Reference
1  [PMID:3821560]
  Authors
Wehland J, Schroder HC, Weber K.
  Title
Isolation and purification of tubulin tyrosine ligase.
  Journal
Methods Enzymol 134:170-9 (1986)
DOI:10.1016/0076-6879(86)34086-2
Reference
2  [PMID:7510228]
  Authors
Rudiger M, Wehland J, Weber K.
  Title
The carboxy-terminal peptide of detyrosinated alpha tubulin provides a minimal system to study the substrate specificity of tubulin-tyrosine ligase.
  Journal
Eur J Biochem 220:309-20 (1994)
DOI:10.1111/j.1432-1033.1994.tb18627.x
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.25
IUBMB Enzyme Nomenclature: 6.3.2.25
ExPASy - ENZYME nomenclature database: 6.3.2.25
BRENDA, the Enzyme Database: 6.3.2.25
CAS: 60321-03-1

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