KEGG   ENZYME: 6.3.2.25Help
Entry
EC 6.3.2.25                 Enzyme                                 

Name
tubulin---tyrosine ligase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
alpha-tubulin:L-tyrosine ligase (ADP-forming)
Reaction(IUBMB)
ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate [RN:R04730]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
detyrosinated alpha-tubulin [CPD:C05234];
L-tyrosine [CPD:C00082]
Product
alpha-tubulin [CPD:C01392];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
L-Tyrosine is linked via a peptide bond to the C-terminus of de-tyrosinated alpha-tubulin (des-Tyromega-alpha-tubulin). The enzyme is highly specific for alpha-tubulin and moderately specific for ATP and L-tyrosine. L-Phenylalanine and 3,4-dihydroxy-L-phenylalanine are transferred but with higher Km values.
History
EC 6.3.2.25 created 1999
Orthology
K06047  tubulin---tyrosine ligase
Genes
HSA: 150465(TTL)
PTR: 470483(TTL)
PPS: 100969421(TTL)
GGO: 101139501
PON: 100450789(TTL)
NLE: 100599199(TTL)
MCC: 699075(TTL)
MCF: 102146185(TTL)
CSAB: 103241269(TTL)
RRO: 104680394(TTL)
RBB: 108518062(TTL)
CJC: 100404587(TTL)
SBQ: 101050847(TTL) 104652891
MMU: 69737(Ttl)
MCAL: 110285528(Ttl) 110295428
MPAH: 110318736(Ttl)
RNO: 171572(Ttl)
MUN: 110551397(Ttl)
CGE: 100755055(Ttl)
NGI: 103739890(Ttl)
HGL: 101701462(Ttl)
OCU: 100353592(TTL)
TUP: 102487597(TTL)
CFA: 483065(TTL)
VVP: 112916643(TTL)
AML: 100474988(TTL)
UMR: 103671867(TTL)
UAH: 113242468(TTL)
ORO: 101380303(TTL)
ELK: 111158497
FCA: 102900797(TTL)
PTG: 102966737(TTL)
PPAD: 109264639(TTL)
AJU: 106976025(TTL)
BTA: 514559(TTL)
BOM: 102280366(TTL)
BIU: 109566127(TTL)
BBUB: 102411049(TTL)
CHX: 102180860(TTL)
OAS: 101107272(TTL)
SSC: 445530(TTL)
CFR: 102509422(TTL)
CDK: 105093106(TTL)
BACU: 103018295(TTL)
LVE: 103087944(TTL)
OOR: 101270645(TTL)
DLE: 111187444(TTL)
PCAD: 102983540(TTL)
ECB: 100052052(TTL)
EPZ: 103559679(TTL)
EAI: 106838551(TTL)
MYB: 102252588(TTL)
MYD: 102760056(TTL)
MNA: 107528635(TTL)
HAI: 109393550(TTL)
DRO: 112303048(TTL)
PALE: 102891762(TTL)
RAY: 107514971(TTL)
MJV: 108401296(TTL)
LAV: 100675880(TTL)
TMU: 101357599
MDO: 100031152(TTL)
SHR: 100930667(TTL)
PCW: 110209200(TTL)
OAA: 100084789(TTL)
GGA: 416711(TTL)
MGP: 100540631(TTL)
CJO: 107310907(TTL)
NMEL: 110395293(TTL)
APLA: 101798438(TTL)
ACYG: 106044757(TTL)
TGU: 100218069(TTL)
LSR: 110472814(TTL)
SCAN: 103821312(TTL)
GFR: 102044350(TTL)
FAB: 101817591(TTL)
PHI: 102110480(TTL)
PMAJ: 107202369(TTL)
CCAE: 111926609(TTL)
CCW: 104696672
ETL: 114066754(TTL)
FPG: 101921873(TTL)
FCH: 102046034(TTL)
CLV: 102097117(TTL)
EGZ: 104128802(TTL)
NNI: 104015810(TTL)
ACUN: 113477983(TTL)
PADL: 103923112(TTL)
AAM: 106494133(TTL)
ASN: 102387034(TTL)
AMJ: 102565195(TTL)
PSS: 102459357(TTL)
CMY: 102931299(TTL)
CPIC: 101933325(TTL)
ACS: 100561131(ttl)
PVT: 110087024(TTL)
PBI: 103067995(TTL)
PMUR: 107296307(TTL)
TSR: 106537996(TTL)
PMUA: 114594790(TTL)
GJA: 107114599(TTL)
XLA: 108716622(ttl.L)
XTR: 100135000(ttl)
NPR: 108801118(TTL)
DRE: 767687(ttl)
SGH: 107577318 107582496(ttl)
IPU: 108263154(ttl)
PHYP: 113545255(ttl)
AMEX: 103045419(ttl)
EEE: 113587313(ttl)
TRU: 101075885(ttl)
LCO: 104921860(ttl)
NCC: 104949570(ttl)
MZE: 101486420(ttl)
ONL: 100706993(ttl)
OLA: 101168672(ttl)
XMA: 102227655(ttl)
XCO: 114138410(ttl)
PRET: 103477148(ttl)
CVG: 107088142(ttl)
NFU: 107375537(ttl)
KMR: 108247179(ttl)
ALIM: 106525593(ttl)
AOCE: 111573098(ttl)
CSEM: 103387495(ttl)
POV: 109633843(ttl)
LCF: 108886280(ttl)
SDU: 111220626(ttl)
SLAL: 111669486(ttl)
HCQ: 109523162(ttl)
BPEC: 110168527(ttl)
MALB: 109962255(ttl)
ELS: 105029715(ttl)
SFM: 108925774(ttl)
PKI: 111849582(ttl)
LCM: 102348001(TTL)
CMK: 103178932(ttl)
RTP: 109936552(ttl)
SPU: 582881
APLC: 110983169
SKO: 100372948
CRG: 105332425
LAK: 106165091
EPA: 110249149
ADF: 107353067
AMIL: 114953547
PDAM: 113674428
SPIS: 111318832
VCN: VOLCADRAFT_105441(ttlD)
SCE: YBR094W(PBY1)
KMX: KLMA_70330(PBY1)
NCS: NCAS_0I01240(NCAS0I01240)
NDI: NDAI_0A07760(NDAI0A07760)
TPF: TPHA_0A03720(TPHA0A03720)
TBL: TBLA_0B02540(TBLA0B02540)
TDL: TDEL_0C04770(TDEL0C04770)
KAF: KAFR_0G01640(KAFR0G01640)
PIC: PICST_74933(YBU4)
CAL: CAALFM_C401730CA(CaO19.4621)
SLB: AWJ20_3514(PBY1) AWJ20_3515(PBY1)
NCR: NCU04743
NTE: NEUTE1DRAFT87071(NEUTE1DRAFT_87071)
MGR: MGG_02486
SSCK: SPSK_01002
MAW: MAC_02712
MAJ: MAA_02096
CMT: CCM_04038
MBE: MBM_09677
ANI: AN4967.2
ANG: ANI_1_574144(An16g04080)
CNE: CNL06170
CNB: CNBI0650
ABP: AGABI1DRAFT110465(AGABI1DRAFT_110465) AGABI1DRAFT66676(AGABI1DRAFT_66676)
ABV: AGABI2DRAFT189636(AGABI2DRAFT_189636) AGABI2DRAFT197617(AGABI2DRAFT_197617)
MGL: MGL_1845
TCR: 507801.50
LFA: LFA_0156
LHA: LHA_3048
TMC: LMI_0200
 » show all
Taxonomy
Reference
1  [PMID:3821560]
  Authors
Wehland J, Schroder HC, Weber K.
  Title
Isolation and purification of tubulin tyrosine ligase.
  Journal
Methods Enzymol 134:170-9 (1986)
Reference
2  [PMID:7510228]
  Authors
Rudiger M, Wehland J, Weber K.
  Title
The carboxy-terminal peptide of detyrosinated alpha tubulin provides a minimal system to study the substrate specificity of tubulin-tyrosine ligase.
  Journal
Eur J Biochem 220:309-20 (1994)
DOI:10.1111/j.1432-1033.1994.tb18627.x
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.25
IUBMB Enzyme Nomenclature: 6.3.2.25
ExPASy - ENZYME nomenclature database: 6.3.2.25
BRENDA, the Enzyme Database: 6.3.2.25
CAS: 60321-03-1

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