KEGG ENZYME: 6.3.2.26

ENZYME: 6.3.2.26

Entry

EC 6.3.2.26                 Enzyme

Name

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase;
L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine synthetase;
ACV synthetase;
L-alpha-aminoadipyl-cysteinyl-valine synthetase

Class

Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)

Sysname

L-2-aminohexanedioate:L-cysteine:L-valine ligase (AMP-forming, valine-inverting)

Reaction(IUBMB)

3 ATP + L-2-aminohexanedioate + L-cysteine + L-valine + H2O = 3 AMP + 3 diphosphate + N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine [RN:R04870]

Reaction(KEGG)

R04870

Substrate

ATP [CPD:C00002];
L-2-aminohexanedioate [CPD:C00956];
L-cysteine [CPD:C00097];
L-valine [CPD:C00183];
H2O [CPD:C00001]

Product

AMP [CPD:C00020];
diphosphate [CPD:C00013];
N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine [CPD:C05556]

Comment

Requires Mg2+. The enzyme contains 4'-phosphopantetheine, which may be involved in the mechanism of the reaction. Forms part of the penicillin biosynthesis pathway (for pathway, click here).

History

EC 6.3.2.26 created 2002

Pathway

ec00311

Penicillin and cephalosporin biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K12743

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase

Genes

BCOO:

119075341

PMEO:

129593796 129595266

PCHM:

VFPPC_08658

ANI:	ANIA_02621

AOR:	AO090038000543

AFV:	AFLA_009029

APUU:

APUU_12364S

ABE:

ARB_02226

TVE:

TRV_00553

XRY:	QN244_11740

SCLF:

BB341_07790

STIR:

DDW44_23095

SCT:	SCAT_5684(pcbAB)

SCY:	SCATT_56840

AAB:	A4R43_03220

AMYY:

YIM_39185(srfAA)

PSUU:

Psuf_059700

» show all

Reference

1 [PMID:11851475]

Authors

Byford MF, Baldwin JE, Shiau CY, Schofield CJ.

Title

The Mechanism of ACV Synthetase.

Journal

Chem Rev 97:2631-2650 (1997)
DOI:10.1021/cr960018l

Reference

2 [PMID:9355751]

Authors

Theilgaard HB, Kristiansen KN, Henriksen CM, Nielsen J.

Title

Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum.

Journal

Biochem J 327 ( Pt 1):185-91 (1997)
DOI:10.1042/bj3270185

Other DBs

ExplorEnz - The Enzyme Database:

6.3.2.26

IUBMB Enzyme Nomenclature:

6.3.2.26

ExPASy - ENZYME nomenclature database:

6.3.2.26

BRENDA, the Enzyme Database:

6.3.2.26

CAS:	57219-73-5

All links

Protein sequence (130)   
   UniProt (98)   
   SWISS-PROT (6)   
   RefSeq(pep) (18)   
   PDBSTR (8)   
DNA sequence (103)   
   RefSeq(nuc) (39)   
   GenBank (37)   
   EMBL (27)   
3D Structure (2)   
   PDB (2)   
Protein domain (14)   
   InterPro (14)   
All databases (249)   

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