EC                 Enzyme                                 

cyanophycin synthase (L-arginine-adding);
CphA (ambiguous);
CphA1 (ambiguous);
CphA2 (ambiguous);
cyanophycin synthetase (ambiguous);
multi-L-arginyl-poly-L-aspartate synthase (ambiguous)
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
cyanophycin:L-arginine ligase (ADP-forming)
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 [RN:R09593]
ATP [CPD:C00002];
[L-Asp(4-L-Arg)]n-L-Asp [CPD:C19710];
L-Arg [CPD:C00062]
ADP [CPD:C00008];
phosphate [CPD:C00009];
[L-Asp(4-L-Arg)]n+1 [CPD:C19713]
Requires Mg2+ for activity. Both this enzyme and EC, cyanophycin synthase (L-aspartate-adding), are required for the elongation of cyanophycin, which is a protein-like cell inclusion that is unique to cyanobacteria and acts as a temporary nitrogen store [2]. Both enzymes are found in the same protein but have different active sites [2,4]. Both L-Asp and L-Arg must be present before either enzyme will display significant activity [2]. Canavanine and lysine can be incoporated into the polymer instead of arginine [2].
EC created 2007
K03802  cyanophycin synthetase
TCI: A7K98_11365
PSD: DSC_11330
LCP: LC55x_4408(cphA)
LGU: LG3211_4215(cphA)
DKO: I596_619
ACI: ACIAD1279(cphA)
AEI: AOY20_08725
CPS: CPS_1871
COZ: A3Q34_13310
COLW: A3Q33_11470
COLA: DBO93_03055(cphA)
GNI: GNIT_3034(cphA)
GPS: C427_4660(cphA)
LLO: LLO_2537(cphA)
LHA: LHA_3073(cphA)
LCD: clem_01105(cphA_1)
TMC: LMI_0180
FTU: FTT_1130c(cphA)
FTQ: RO31_1310(cphA)
FTF: FTF1130c(cphA)
FTW: FTW_1163(cphA)
FTT: FTV_1080(cphA)
FTG: FTU_1164(cphA)
FTL: FTL_0831
FTH: FTH_0821(cphA)
FTA: FTA_0880
FTS: F92_04580
FTI: FTS_0821(cphA)
FTC: DA46_1978(cphA)
FTV: CH67_1166(cphA)
FTZ: CH68_897(cphA)
FTM: FTM_1298(cphA)
FTN: FTN_1112(cphA)
FTX: AW25_896(cphA)
FTD: AS84_1407(cphA)
FTY: CH70_826(cphA)
FPH: Fphi_1494
FPT: BZ13_486(cphA)
FPI: BF30_1763(cphA)
FPM: LA56_658(cphA)
FPX: KU46_1809(cphA)
FPZ: LA55_728(cphA)
FPJ: LA02_1737(cphA)
FNA: OOM_1291
FHA: CDV26_03650(cphA)
MEC: Q7C_408
GAI: IMCC3135_14330(cphA_3)
CSA: Csal_1114
HCO: LOKO_00953(cphA_1) LOKO_03364(cphA_2)
EBH: BSEPE_0471(cphA)
REH: H16_A0774(cphA) H16_A0775(cphA')
CNC: CNE_1c07850(cphA1) CNE_1c07860(cphA2)
RME: Rmet_0703(cphA1) Rmet_0704(cphA2)
CGD: CR3_0639(cphA) CR3_0640(cphA)
BPE: BP1739(cphA) BP1740(cphA)
BPC: BPTD_1717(cphA) BPTD_1718(cphA)
BPER: BN118_2118(cphA) BN118_2119(cphA)
BPET: B1917_2094(cphA) B1917_2095(cphA)
BPEU: Q425_19170(cphA) Q425_19180(cphA)
BPA: BPP3184(cphA)
BPAR: BN117_3147(cphA) BN117_3148(cphA)
BBR: BB3584(cphA) BB3585(cphA)
BBM: BN115_3307(cphA) BN115_3308(cphA)
BBH: BN112_4779(cphA) BN112_4780(cphA)
BBX: BBS798_3415(cphA) BBS798_3416(cphA)
BPT: Bpet1860(cphA1) Bpet1861(cphA2)
BAV: BAV1232(cphA1) BAV1233(cphA2)
BHZ: ACR54_03186(cphA_1) ACR54_03187(cphA_2)
AXY: AXYL_01677(cphA1) AXYL_01678(cphA2)
AXX: ERS451415_01577(cphA_1) ERS451415_01578(cphA_2)
ASW: CVS48_27620(cphA) CVS48_27625(cphA)
ACHR: C2U31_13685(cphA) C2U31_13690(cphA)
ACRA: BSY15_2233(cphA) BSY15_2234(cphA)
VPD: VAPA_1c11110(cphA1) VAPA_1c11120(cphA2)
VBO: CKY39_05240(cphA) CKY39_05245(cphA)
VAM: C4F17_13905(cphA) C4F17_13910(cphA)
OTK: C6570_11090(cphA) C6570_11095(cphA)
LIM: L103DPR2_01456(cphA_1) L103DPR2_01457(cphA_2)
LIH: L63ED372_00969(cphA_2) L63ED372_00970(cphA_3)
SIMP: C6571_04305(cphA) C6571_04310(cphA)
MELM: C7H73_14610(cphA) C7H73_14615(cphA)
MELA: C6568_05025(cphA) C6568_05030(cphA)
MMS: mma_3555(cphA1) mma_3556(cphA2)
JAG: GJA_3669(cphA) GJA_3671(cphA)
JSV: CNX70_22610(cphA) CNX70_22615(cphA)
HSE: Hsero_2170(cphA2) Hsero_2171(cphA1)
HRB: Hrubri_2052(cphA2) Hrubri_2053(cphA1)
HEE: hmeg3_09900(cphA) hmeg3_09905(cphA)
CFU: CFU_1324(cphA1) CFU_1325(cphA2)
CPRA: CPter91_3701(cphA) CPter91_3702(cphA)
MASS: CR152_16020(cphA) CR152_16025(cphA) CR152_19690(cphA) CR152_26900(cphA)
MASZ: C9I28_13485(cphA) C9I28_14925(cphA) C9I28_14930(cphA) C9I28_15890(cphA)
MTIM: DIR46_01320(cphA) DIR46_01330(cphA) DIR46_20250(cphA) DIR46_20255(cphA)
RGE: RGE_40110(cphA) RGE_40120(cphA)
AON: DEH84_05155(cphA) DEH84_05160(cphA)
PBH: AAW51_1165(cphA) AAW51_3629(cphA) AAW51_3630
NEU: NE0922(cphA) NE0923
NLC: EBAPG3_014370(cphA) EBAPG3_014375(cphA)
SDR: SCD_n00680(cphA1) SCD_n00681
ZPA: C3497_00070(cphA) C3497_00075(cphA)
PPD: Ppro_1989
DTO: TOL2_C09850(cphA)
RLG: Rleg_5803
OAR: OA238_c34280(cphA)
THAS: C6Y53_02585(cphA)
SMAZ: LH19_11820
SINB: SIDU_15095
BMET: BMMGA3_04930(cphA)
BKW: BkAM31D_21445(gshAB_1) BkAM31D_21515(cphA_3)
PPOL: X809_04480
AAC: Aaci_0173
AAD: TC41_0240(cphA)
BTS: Btus_0063
KYR: CVV65_00340(cphA)
TAB: CIG75_20325(cphA)
CPE: CPE2213
CPF: CPF_2477(cphA)
CPR: CPR_2187(cphA)
CTC: CTC_00282
CBO: CBO0120(cphA)
CBA: CLB_0156(cphA)
CBH: CLC_0168(cphA)
CBY: CLM_0163(cphA)
CBL: CLK_3295(cphA)
CBK: CLL_A0470(cphA)
CBB: CLD_0666(cphA)
CBI: CLJ_B0158(cphA)
CBT: CLH_0462(cphA)
CBF: CLI_0175(cphA)
CBM: CBF_0148(cphA)
CBE: Cbei_0393
CBZ: Cbs_0393
CBEI: LF65_00451
CKL: CKL_3725(cphA)
CKR: CKR_3289
CLJ: CLJU_c02100(cphA)
CSR: Cspa_c05990(cphA)
CBV: U729_1602(cphA)
CSQ: CSCA_3950
CLD: CLSPO_c01450(cphA)
CACE: CACET_c06280(cphA)
CTYK: CTK_C01190
CTAE: BGI42_02120(cphA)
AMT: Amet_1227
AOE: Clos_2106
ASF: SFBM_1294
ASM: MOUSESFB_1203(cphA)
ASO: SFBmNL_01365(cphA)
ASB: RATSFB_1122(cphA)
CTH: Cthe_2204
CCE: Ccel_0132
CCEL: CCDG5_1423
CTHD: CDO33_00890(cphA)
SWO: Swol_1350
DSY: DSY0146
DHD: Dhaf_0085
SGY: Sgly_0189
DED: DHBDCA_p256(cphA)
DEC: DCF50_p316(cphA)
SAY: TPY_0184(cphA)
CTHM: CFE_0043
TTE: TTE2785(MurE3)
THX: Thet_2447
TIT: Thit_2390
TKI: TKV_c24650(cphA)
TPZ: Tph_c28390(cphA)
CSC: Csac_0753
ATE: Athe_0527
TOC: Toce_0042
TTM: Tthe_2740
TSH: Tsac_0535
CAD: Curi_c07930(cphA)
MMI: MMAR_2406
MPH: MLP_14760(cphA)
NDK: I601_3383(cphA_1)
KFL: Kfla_3708
NML: Namu_4854
ASE: ACPL_6520(cphA)
ACTN: L083_6049(cphA)
AFS: AFR_30950
PLAB: C6361_21775(cphA)
PLAT: C6W10_16735(cphA)
SYN: slr2002
SYY: SYNGTS_1314(cphA)
SYT: SYNGTI_1314(cphA)
SYS: SYNPCCN_1313(cphA)
SYQ: SYNPCCP_1313(cphA)
SYJ: D082_30240(cphA)
SYO: C7I86_06805(cphA)
SYR: SynRCC307_1724(cphA)
SYX: SynWH7803_1936(cphA)
SYP: SYNPCC7002_A2405(cphA)
CYB: CYB_0911(cphA) CYB_2909
SYND: KR52_01410
TEL: tlr2170
THN: NK55_07505(cphA)
LET: O77CONTIG1_02520(cphA_1) O77CONTIG1_04203(cphA_2)
MAR: MAE_27460(cphA)
CYL: AA637_09500(cphA)
CYT: cce_2237(cphA)
TER: Tery_1965
ARP: NIES39_D05270(cphA)
GVI: gvip562(cphA)
GLJ: GKIL_2419(cphA)
ANA: all3879(cphA) alr0573(cphA)
NAZ: Aazo_1178
NSP: BMF81_02103(cphA)
HAU: Haur_0617
TRO: trd_0739
STI: Sthe_1298
PIR: VN12_09645(cphA_1) VN12_19140(cphA_2)
PLS: VT03_04560(cphA)
PGS: CPT03_19605(cphA)
MUC: MuYL_0808
DFE: Dfer_0515
SLI: Slin_4152
HSW: Hsw_3070
GFO: GFO_0745(cphA)
FPS: FP1111(cphA)
LACI: CW733_14455(cphA)
NDO: DDD_3459(cphA)
CHZ: CHSO_2860(cphA)
CIO: CEQ15_07900(cphA)
CHRY: CEY12_05200(cphA)
CPIP: CJF12_02880(cphA)
WIN: WPG_0938
AUE: C5O00_03750(cphA)
FBA: FIC_01365
FLU: CHH17_05595(cphA)
CCH: Cag_0947
 » show all
1  [PMID:11131019]
Aboulmagd E, Oppermann-Sanio FB, Steinbuchel A.
Molecular characterization of the cyanophycin synthetase from Synechocystis sp. strain PCC6308.
Arch Microbiol 174:297-306 (2000)
2  [PMID:11319097]
Aboulmagd E, Oppermann-Sanio FB, Steinbuchel A.
Purification of Synechocystis sp. strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity.
Appl Environ Microbiol 67:2176-82 (2001)
3  [PMID:6767688]
Allen MM, Hutchison F, Weathers PJ.
Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308.
J Bacteriol 141:687-93 (1980)
4  [PMID:10951215]
Berg H, Ziegler K, Piotukh K, Baier K, Lockau W, Volkmer-Engert R.
Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin): mechanism of the cyanophycin synthetase reaction studied with synthetic primers.
Eur J Biochem 267:5561-70 (2000)
5  [PMID:12132696]
Ziegler K, Deutzmann R, Lockau W.
Cyanophycin synthetase-like enzymes of non-cyanobacterial eubacteria: characterization of the polymer produced by a recombinant synthetase of Desulfitobacterium hafniense.
Z Naturforsch [C] 57:522-9 (2002)
6  [PMID:9652408]
Ziegler K, Diener A, Herpin C, Richter R, Deutzmann R, Lockau W.
Molecular characterization of cyanophycin synthetase, the enzyme catalyzing the biosynthesis of the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartate (cyanophycin).
Eur J Biochem 254:154-9 (1998)
Other DBs
ExplorEnz - The Enzyme Database:
IUBMB Enzyme Nomenclature:
ExPASy - ENZYME nomenclature database:
BRENDA, the Enzyme Database:
CAS: 131554-17-1

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