KEGG ENZYME: 6.3.2.38

ENZYME: 6.3.2.38

Entry

EC 6.3.2.38 Enzyme

Name

N2-citryl-N6-acetyl-N6-hydroxylysine synthase;
Nalpha-citryl-Nepsilon-acetyl-Nepsilon-hydroxylysine synthase;
iucA (gene name)

Class

Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)

Sysname

citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (ADP-forming)

Reaction(IUBMB)

2 ATP + citrate + N6-acetyl-N6-hydroxy-L-lysine + H2O = 2 ADP + 2 phosphate + N2-citryl-N6-acetyl-N6-hydroxy-L-lysine [RN:R10090]

Reaction(KEGG)

R10090

Substrate

ATP [CPD:C00002];
citrate [CPD:C00158];
N6-acetyl-N6-hydroxy-L-lysine [CPD:C03955];
H2O [CPD:C00001]

Product

ADP [CPD:C00008];
phosphate [CPD:C00009];
N2-citryl-N6-acetyl-N6-hydroxy-L-lysine [CPD:C20333]

Comment

Requires Mg2+. Aerobactin is one of a group of high-affinity iron chelators known as siderophores and is produced under conditions of iron deprivation [5]. It is a dihydroxamate comprising two molecules of N6-acetyl-N6-hydroxy-L-lysine and one molecule of citrate. This enzyme catalyses the first of two synthase reactions to link N6-acetyl-N6-hydroxy-L-lysine and citrate [4,5].

History

EC 6.3.2.38 created 2012

Pathway

ec00310

Lysine degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K03894

N2-citryl-N6-acetyl-N6-hydroxylysine synthase

Genes

EOJ:	ECO26_1345(iucA)

EOI:	ECO111_1293(iucA)

ECC:	c3627(iucA)

ECV:	APECO1_O1CoBM172(iucA)

ECM:	EcSMS35_3182(iucA)

ECQ:	ECED1_4905(iucA)

ECK:	EC55989_4645(iucA)

ECT:	ECIAI39_3423(iucA)

EOC:	CE10_3446(iucA)

EUM:	ECUMN_3375(iucA)

ECZ:	ECS88_p0013(iucA)

ELN:	NRG857_30241(iucA)

ESO:

O3O_02270

ESM:

O3M_23000

ESL:

O3K_23085

EAB:	ECABU_c32790(iucA)

ENA:	ECNA114_4701(iucA)

ELC:	i14_3320(iucA)

ELD:	i02_3320(iucA)

ECOJ:

P423_16360

ECOO:

ECRM13514_1290(iucA)

ECOH:

ECRM13516_1246(iucA)

ECOS:

EC958_3271(iucA)

SEM:	STMDT12_C38570

SES:	SARI_02152

SFL:	SF3715(iucA)

SFX:	S4056(iucA)

SFV:	SFV_3854(iucA)

SFE:

SFxv_4048

SFN:

SFy_5294

SFS:

SFyv_5368

SFT:	NCTC1_04012(iucA)

SSN:	SSON_3601(iucA)

SBO:	SBO_4337(iucA)

SBC:	SbBS512_E4634(iucA)

ENC:	ECL_03316(iucA)

ENO:	ECENHK_14300

ECLO:

ENC_41860

EEC:	EcWSU1_02919(iucA)

ENL:	A3UG_14855

ECLG:

ECLE:

ECLN:

ECLI:

ECLX:

ECLY:

ECLZ:

ECLA:

ECLC:

EAU:	DI57_04930

EHM:	AB284_11235

EKB:	BFV64_14410

ELG:	BH714_10650

EXF:	BFV63_14005

ECLS:

LI67_011590

ENX:	NI40_014270

ESA:	ESA_pESA3p05547

CSZ:	CSSP291_21018

CSJ:	CSK29544_1p0029

CCON:

AFK62_20050

CDM:	AFK67_20990

CSI:	P262_p1005

CMJ:	AFK66_020195

CUI:	AFK65_20180

CTU:	Ctu_1p01040(iucA)

KPO:	KPN2242_25281

KPR:	KPR_4897(iucA)

KPNK:

BN49_pII0068(iucA)

KMI:	VW41_08320

CKO:

CKO_00974

CNT:	JT31_04195

CEM:	LH23_13825

CEN:	LH86_13135

LNI:	CWR52_20785

YPK:	y3380(iucA1) y3381(iucA2)

YPA:	YPA_0274 YPA_0275

YPN:	YPN_3187 YPN_3188

YPP:	YPDSF_0528 YPDSF_0529

YPZ:	YPZ3_0748(iucA2) YPZ3_0749(iucA1)

YPT:	A1122_00025 A1122_00030

YPD:	YPD4_0703(iucA2) YPD4_0704(iucA1)

YPX:	YPD8_0703(iucA2) YPD8_0704(iucA1)

YPH:	YPC_0802 YPC_0803

YPW:	CH59_873 CH59_874

YPJ:	CH55_3550 CH55_3551

YPV:	BZ15_2583 BZ15_2584

YPL:	CH46_4155 CH46_4156

YPS:	YPTB3263(rhbC)

YPO:

BZ17_3347

YPI:	YpsIP31758_0714(iucA)

YPY:

YPB:

YPQ:

YPU:

YPR:

YPC:

YPF:

YSI:	BF17_03335

YIN:

CH53_2437

YAK:	ACZ76_16810

YMA:	DA391_18835

SPE:

Spro_0922

SRR:	SerAS9_0847

SRL:	SOD_c07780(iucA)

SRY:	M621_04290

SPLY:

Q5A_004140(iucA)

SRS:	SerAS12_0847

SRA:	SerAS13_0847

SMAF:

D781_0887

SLQ:	M495_03895

SFW:	WN53_13470

SFG:	AV650_09020

SRZ:	AXX16_4214

PWA:	Pecwa_0947

PPAR:

A8F97_14195

PEC:

W5S_0838

EGE:	EM595_p0526(iucA)

PAM:	PANA_0349(iucA)

PLF:	PANA5342_4064(iucA)

PAJ:	PAJ_3504(iucA)

PAQ:	PAGR_g3927

PSTW:

DSJ_03475

VCY:	IX92_17045

VCT:	JV59_21980

VMI:	AL543_14510

VSH:	BSZ05_19015

VFI:	VF_A0161(iucA)

VFM:	VFMJ11_A0197

GHO:	AL542_16550

SWD:

SPOI:

NOC:

NWA:

KUS:	B9G99_09235

KMA:	B9H00_05615

KAK:	Kalk_08740

KKO:

Kkor_2043

KGE:

TQ33_0710

CCRO:

CMC5_023400(iucA)

BBA:	Bd1576(iucA)

BBAT:

Bdt_1569(iucA)

BBAC:

BMX:

BSTO:

OAN:

OAH:

BVV:	BHK69_19680

MET:

M446_5062

GBE:	GbCGDNIH1_2378

GBC:	GbCGDNIH3_2378

SAO:	SAOUHSC_00079

» show all

Reference

Authors

Appanna, D.L., Grundy, B.J., Szczepan, E.W. and Viswanatha, T.

Title

Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1.

Journal

Biochim Biophys Acta 801:437-443 (1984)

Reference

2 [PMID:4313071]

Authors

Gibson F, Magrath DI.

Title

The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I.

Journal

Biochim Biophys Acta 192:175-84 (1969)
DOI:10.1016/0304-4165(69)90353-5

Reference

Authors

Maurer, P.J. and Miller, M.

Title

Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine.

Journal

J Am Chem Soc 104:3096-3101 (1982)

Reference

4 [PMID:2935523]

Authors

de Lorenzo V, Bindereif A, Paw BH, Neilands JB.

Title

Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.

Journal

J Bacteriol 165:570-8 (1986)
DOI:10.1128/JB.165.2.570-578.1986

Sequence

[ag:CAA53707]

Reference

5 [PMID:15719346]

Authors

Challis GL.

Title

A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases.

Journal

Chembiochem 6:601-11 (2005)
DOI:10.1002/cbic.200400283

Other DBs

ExplorEnz - The Enzyme Database:

6.3.2.38

IUBMB Enzyme Nomenclature:

6.3.2.38

ExPASy - ENZYME nomenclature database:

6.3.2.38

BRENDA, the Enzyme Database:

6.3.2.38

DBGET integrated database retrieval system