KEGG   ENZYME: 6.3.2.38Help
Entry
EC 6.3.2.38                 Enzyme                                 

Name
N2-citryl-N6-acetyl-N6-hydroxylysine synthase;
Nalpha-citryl-Nepsilon-acetyl-Nepsilon-hydroxylysine synthase;
iucA (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (ADP-forming)
Reaction(IUBMB)
2 ATP + citrate + N6-acetyl-N6-hydroxy-L-lysine + H2O = 2 ADP + 2 phosphate + N2-citryl-N6-acetyl-N6-hydroxy-L-lysine [RN:R10090]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
citrate [CPD:C00158];
N6-acetyl-N6-hydroxy-L-lysine [CPD:C03955];
H2O [CPD:C00001]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
N2-citryl-N6-acetyl-N6-hydroxy-L-lysine [CPD:C20333]
Comment
Requires Mg2+. Aerobactin is one of a group of high-affinity iron chelators known as siderophores and is produced under conditions of iron deprivation [5]. It is a dihydroxamate comprising two molecules of N6-acetyl-N6-hydroxy-L-lysine and one molecule of citrate. This enzyme catalyses the first of two synthase reactions to link N6-acetyl-N6-hydroxy-L-lysine and citrate [4,5].
History
EC 6.3.2.38 created 2012
Pathway
ec00310  Lysine degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K03894  N2-citryl-N6-acetyl-N6-hydroxylysine synthase
Genes
EOJ: ECO26_1345(iucA)
EOI: ECO111_1293(iucA)
ECC: c3627(iucA)
ECV: APECO1_O1CoBM172(iucA)
ECM: EcSMS35_3182(iucA)
ECQ: ECED1_4905(iucA)
ECK: EC55989_4645(iucA)
ECT: ECIAI39_3423(iucA)
EOC: CE10_3446(iucA)
EUM: ECUMN_3375(iucA)
ECZ: ECS88_p0013(iucA)
ELN: NRG857_30241(iucA)
ESO: O3O_02270
ESM: O3M_23000
ESL: O3K_23085
EAB: ECABU_c32790(iucA)
ENA: ECNA114_4701(iucA)
ELC: i14_3320(iucA)
ELD: i02_3320(iucA)
ECOJ: P423_16360
ECOO: ECRM13514_1290(iucA)
ECOH: ECRM13516_1246(iucA)
ECOS: EC958_3271(iucA)
SFL: SF3715(iucA)
SFX: S4056(iucA)
SFV: SFV_3854(iucA)
SFE: SFxv_4048
SFN: SFy_5294
SFS: SFyv_5368
SFT: NCTC1_04012(iucA)
SSN: SSON_3601(iucA)
SBO: SBO_4337(iucA)
SBC: SbBS512_E4634(iucA)
ENC: ECL_03316(iucA)
ECLO: ENC_41860
EEC: EcWSU1_02919(iucA)
ECLX: LI66_14260
ECLY: LI62_15605
ECLZ: LI64_13670
CTU: Ctu_1p01040(iucA)
KPR: KPR_4897(iucA)
KPNK: BN49_pII0068(iucA)
CKO: CKO_00974
YPK: y3380(iucA1) y3381(iucA2)
YPZ: YPZ3_0748(iucA2) YPZ3_0749(iucA1)
YPD: YPD4_0703(iucA2) YPD4_0704(iucA1)
YPX: YPD8_0703(iucA2) YPD8_0704(iucA1)
YPS: YPTB3263(rhbC)
YPO: BZ17_3347
YPI: YpsIP31758_0714(iucA)
YPY: YPK_0786
YPB: YPTS_3399
YPQ: DJ40_3174
YPU: BZ21_2543
YPR: BZ20_2851
YPC: BZ23_2824
YPF: BZ19_2669
YIN: CH53_2437
SPE: Spro_0922
SRL: SOD_c07780(iucA)
SPLY: Q5A_004140(iucA)
SMAF: D781_0887
PEC: W5S_0838
EGE: EM595_p0526(iucA)
PAM: PANA_0349(iucA)
PLF: PANA5342_4064(iucA)
PAJ: PAJ_3504(iucA)
PSTW: DSJ_03475
VFI: VF_A0161(iucA)
SWD: Swoo_0585
NOC: Noc_1814
NWA: Nwat_1299
KKO: Kkor_2043
KGE: TQ33_0710
CCRO: CMC5_023400(iucA)
BBA: Bd1576(iucA)
BBAT: Bdt_1569(iucA)
BBAC: EP01_04100
BMX: BMS_0528
OAN: Oant_3257
OAH: DR92_3232
MET: M446_5062
 » show all
Taxonomy
Reference
1
  Authors
Appanna, D.L., Grundy, B.J., Szczepan, E.W. and Viswanatha, T.
  Title
Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1.
  Journal
Biochim Biophys Acta 801:437-443 (1984)
Reference
2  [PMID:4313071]
  Authors
Gibson F, Magrath DI.
  Title
The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I.
  Journal
Biochim Biophys Acta 192:175-84 (1969)
DOI:10.1016/0304-4165(69)90353-5
Reference
3
  Authors
Maurer, P.J. and Miller, M.
  Title
Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine.
  Journal
J Am Chem Soc 104:3096-3101 (1982)
Reference
4  [PMID:2935523]
  Authors
de Lorenzo V, Bindereif A, Paw BH, Neilands JB.
  Title
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
  Journal
J Bacteriol 165:570-8 (1986)
DOI:10.1128/JB.165.2.570-578.1986
  Sequence
Reference
5  [PMID:15719346]
  Authors
Challis GL.
  Title
A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases.
  Journal
Chembiochem 6:601-11 (2005)
DOI:10.1002/cbic.200400283
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.38
IUBMB Enzyme Nomenclature: 6.3.2.38
ExPASy - ENZYME nomenclature database: 6.3.2.38
BRENDA, the Enzyme Database: 6.3.2.38

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