KEGG   ENZYME: 6.3.2.38Help
Entry
EC 6.3.2.38                 Enzyme                                 

Name
N2-citryl-N6-acetyl-N6-hydroxylysine synthase;
Nalpha-citryl-Nepsilon-acetyl-Nepsilon-hydroxylysine synthase;
iucA (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (AMP-forming)
Reaction(IUBMB)
2 ATP + citrate + N6-acetyl-N6-hydroxy-L-lysine + H2O = 2 ADP + 2 phosphate + N6-acetyl-N2-citryl-N6-hydroxy-L-lysine [RN:R10090]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
citrate [CPD:C00158];
N6-acetyl-N6-hydroxy-L-lysine [CPD:C03955];
H2O [CPD:C00001]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
N6-acetyl-N2-citryl-N6-hydroxy-L-lysine
Comment
Requires Mg2+. The enzyme is involved in the biosynthesis of aerobactin, a dihydroxamate siderophore. It belongs to a class of siderophore synthases known as type A nonribosomal peptide synthase-independent synthases (NIS). Type A enzymes are responsible for the formation of amide or ester bonds between polyamines or amino alcohols and a prochiral carboxyl group of citrate. The enzyme is believed to form an adenylate intermediate prior to ligation.
History
EC 6.3.2.38 created 2012, modified 2019
Pathway
ec00310  Lysine degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03894  N2-citryl-N6-acetyl-N6-hydroxylysine synthase
Genes
EOJ: ECO26_1345(iucA)
EOI: ECO111_1293(iucA)
ECOO: ECRM13514_1290(iucA)
ECOH: ECRM13516_1246(iucA)
ESL: O3K_23085
ESO: O3O_02270
ESM: O3M_23000
ECK: EC55989_4645(iucA)
ENA: ECNA114_4701(iucA)
ECOS: EC958_3271(iucA)
ECV: APECO1_O1CoBM172(iucA)
ECM: EcSMS35_3182(iucA)
ECQ: ECED1_4905(iucA)
EUM: ECUMN_3375(iucA)
ECT: ECIAI39_3423(iucA)
EOC: CE10_3446(iucA)
ECZ: ECS88_p0013(iucA)
ECC: c3627(iucA)
ELN: NRG857_30241(iucA)
EAB: ECABU_c32790(iucA)
ELC: i14_3320(iucA)
ELD: i02_3320(iucA)
ECOJ: P423_16360
SFL: SF3715(iucA)
SFX: S4056(iucA)
SFV: SFV_3854(iucA)
SFE: SFxv_4048
SFN: SFy_5294
SFS: SFyv_5368
SFT: NCTC1_04012(iucA)
SSN: SSON_3601(iucA)
SBO: SBO_4337(iucA)
SBC: SbBS512_E4634(iucA)
ENC: ECL_03316(iucA)
ECLO: ENC_41860
ECLX: LI66_14260
ECLY: LI62_15605
ECLZ: LI64_13670
EEC: EcWSU1_02919(iucA)
CTU: Ctu_1p01040(iucA)
KPU: KP1_p319(iucA)
KPR: KPR_4897(iucA)
KPNK: BN49_pII0068(iucA)
CKO: CKO_00974
CLAP: NCTC11466_02781(iucA)
YPK: y3380(iucA1) y3381(iucA2)
YPZ: YPZ3_0748(iucA2) YPZ3_0749(iucA1)
YPD: YPD4_0703(iucA2) YPD4_0704(iucA1)
YPX: YPD8_0703(iucA2) YPD8_0704(iucA1)
YPS: YPTB3263(rhbC)
YPO: BZ17_3347
YPI: YpsIP31758_0714(iucA)
YPY: YPK_0786
YPB: YPTS_3399
YPQ: DJ40_3174
YPU: BZ21_2543
YPR: BZ20_2851
YPC: BZ23_2824
YPF: BZ19_2669
YIN: CH53_2437
SPE: Spro_0922
SRL: SOD_c07780(iucA)
SPLY: Q5A_004140(iucA)
SMAF: D781_0887
PEC: W5S_0838
EGE: EM595_p0526(iucA)
PAM: PANA_0349(iucA)
PLF: PANA5342_4064(iucA)
PAJ: PAJ_3504(iucA)
PSTW: DSJ_03475
VFI: VF_A0161(iucA)
SWD: Swoo_0585
NOC: Noc_1814
NWA: Nwat_1299
KKO: Kkor_2043
KGE: TQ33_0710
CCRO: CMC5_023400(iucA)
BBA: Bd1576(iucA)
BBAT: Bdt_1569(iucA)
BBAC: EP01_04100
BMX: BMS_0528
HAX: BALOs_0962(iucA)
OAN: Oant_3257
OAH: DR92_3232
MET: M446_5062
 » show all
Taxonomy
Reference
1  [PMID:4313071]
  Authors
Gibson F, Magrath DI.
  Title
The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I.
  Journal
Biochim Biophys Acta 192:175-84 (1969)
DOI:10.1016/0304-4165(69)90353-5
Reference
2
  Authors
Maurer, P.J. and Miller, M.
  Title
Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine.
  Journal
J Am Chem Soc 104:3096-3101 (1982)
Reference
3  [PMID:2935523]
  Authors
de Lorenzo V, Bindereif A, Paw BH, Neilands JB.
  Title
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
  Journal
J Bacteriol 165:570-8 (1986)
DOI:10.1128/JB.165.2.570-578.1986
  Sequence
Reference
4
  Authors
Appanna, D.L., Grundy, B.J., Szczepan, E.W. and Viswanatha, T.
  Title
Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1.
  Journal
Biochim Biophys Acta 801:437-443 (1984)
Reference
5  [PMID:15719346]
  Authors
Challis GL.
  Title
A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases.
  Journal
Chembiochem 6:601-11 (2005)
DOI:10.1002/cbic.200400283
Reference
6  [PMID:19865642]
  Authors
Oves-Costales D, Kadi N, Challis GL
  Title
The long-overlooked enzymology of a nonribosomal peptide synthetase-independent pathway for virulence-conferring siderophore biosynthesis.
  Journal
Chem Commun (Camb) 6530-41 (2009)
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.38
IUBMB Enzyme Nomenclature: 6.3.2.38
ExPASy - ENZYME nomenclature database: 6.3.2.38
BRENDA, the Enzyme Database: 6.3.2.38

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