KEGG   ENZYME: 6.3.2.38
Entry
EC 6.3.2.38                 Enzyme                                 
Name
N2-citryl-N6-acetyl-N6-hydroxylysine synthase;
Nalpha-citryl-Nepsilon-acetyl-Nepsilon-hydroxylysine synthase;
iucA (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (AMP-forming)
Reaction(IUBMB)
2 ATP + citrate + N6-acetyl-N6-hydroxy-L-lysine + H2O = 2 ADP + 2 phosphate + N6-acetyl-N2-citryl-N6-hydroxy-L-lysine [RN:R10090]
Reaction(KEGG)
R10090
Substrate
ATP [CPD:C00002];
citrate [CPD:C00158];
N6-acetyl-N6-hydroxy-L-lysine [CPD:C03955];
H2O [CPD:C00001]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
N6-acetyl-N2-citryl-N6-hydroxy-L-lysine
Comment
Requires Mg2+. The enzyme is involved in the biosynthesis of aerobactin, a dihydroxamate siderophore. It belongs to a class of siderophore synthases known as type A nonribosomal peptide synthase-independent synthases (NIS). Type A enzymes are responsible for the formation of amide or ester bonds between polyamines or amino alcohols and a prochiral carboxyl group of citrate. The enzyme is believed to form an adenylate intermediate prior to ligation.
History
EC 6.3.2.38 created 2012, modified 2019
Pathway
ec00997  Biosynthesis of various other secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K03894  N2-citryl-N6-acetyl-N6-hydroxylysine synthase
Genes
EOIECO111_1293(iucA)
EOJECO26_1345(iucA)
ECOOECRM13514_1290(iucA)
ECOHECRM13516_1246(iucA)
ESLO3K_23085
ESOO3O_02270
ESMO3M_23000
ECKEC55989_4645(iucA)
ENAECNA114_4701(iucA)
ECOSEC958_3271(iucA)
ECVAPECO1_O1CoBM172(iucA)
ECMEcSMS35_3182(iucA)
ECQECED1_4905(iucA)
EUMECUMN_3375(iucA)
ECTECIAI39_3423(iucA)
EOCCE10_3446(iucA)
ECZECS88_p0013(iucA)
ECCc3627(iucA)
ELNNRG857_30241(iucA)
EABECABU_c32790(iucA)
ELCi14_3320(iucA)
ELDi02_3320(iucA)
ECOJP423_16360
SEMSTMDT12_C38570
SESSARI_02152
SFLSF3715(iucA)
SFXS4056(iucA)
SFVSFV_3854(iucA)
SFESFxv_4048
SFNSFy_5294
SFSSFyv_5368
SFTNCTC1_04012(iucA)
SSNSSON_3601(iucA)
SBOSBO_4337(iucA)
SBCSbBS512_E4634(iucA)
ENCECL_03316(iucA)
ENLA3UG_14855
ECLGEC036_28760
ECLEECNIH2_14820
ECLNECNIH4_08335
ECLIECNIH5_13740
ECLXLI66_14260
ECLYLI62_15605
ECLZLI64_13670
ECLOENC_41860
EHMAB284_11235
EXFBFV63_14005
ECLAECNIH3_13795
ECLCECR091_13730
EAUDI57_04930
EKBBFV64_14410
ENOECENHK_14300
EECEcWSU1_02919(iucA)
ELGBH714_10650
ERNBFV67_13920
ECLSLI67_011590
ECHGFY206_15830
ESHC1N69_14685
ENXNI40_014270
LNICWR52_20785
EBGFAI37_14870
ENDA4308_18985
ENZG0034_14890
ENSHWQ15_22470
ENKLOC22_02250
ESAESA_pESA3p05547
CSZCSSP291_21018
CSJCSK29544_1p0029
CCONAFK62_20050
CDMAFK67_20990
CSIP262_p1005
CMJAFK66_020195
CUIAFK65_20180
CTUCtu_1p01040(iucA)
KPUKP1_p319(iucA)
KPOKPN2242_25281
KPRKPR_4897(iucA)
KPNKBN49_pII0068(iucA)
KMIVW41_08320
CKOCKO_00974
CNTJT31_04195
CEMLH23_13825
CENLH86_13135
CLAPNCTC11466_02781(iucA)
YREHEC60_04375
YPKy3380(iucA1) y3381(iucA2)
YPHYPC_0802 YPC_0803
YPAYPA_0274 YPA_0275
YPNYPN_3187 YPN_3188
YPPYPDSF_0528 YPDSF_0529
YPZYPZ3_0748(iucA2) YPZ3_0749(iucA1)
YPTA1122_00025 A1122_00030
YPDYPD4_0703(iucA2) YPD4_0704(iucA1)
YPXYPD8_0703(iucA2) YPD8_0704(iucA1)
YPWCH59_873 CH59_874
YPJCH55_3550 CH55_3551
YPVBZ15_2583 BZ15_2584
YPLCH46_4155 CH46_4156
YPSYPTB3263(rhbC)
YPOBZ17_3347
YPIYpsIP31758_0714(iucA)
YPYYPK_0786
YPBYPTS_3399
YPQDJ40_3174
YPUBZ21_2543
YPRBZ20_2851
YPCBZ23_2824
YPFBZ19_2669
YSIBF17_03335
YINCH53_2437
YAKACZ76_16810
YMADA391_18835
YMOHRD69_17550
SPESpro_0922
SRRSerAS9_0847
SRLSOD_c07780(iucA)
SRYM621_04290
SPLYQ5A_004140(iucA)
SRSSerAS12_0847
SRASerAS13_0847
SMAFD781_0887
SLQM495_03895
SFWWN53_13470
SFGAV650_09020
SRZAXX16_4214
SERMCLM71_04300
SQUE4343_11890
SFJSAMEA4384070_0954(iucA)
SOFNCTC11214_00578(iucA)
SRHZFO014_05480
FSMCCS41_02990
PWAPecwa_0947
PPARA8F97_14195
PECW5S_0838
BRBEH207_14265
EGEEM595_p0526(iucA)
PAMPANA_0349(iucA)
PLFPANA5342_4064(iucA)
PAJPAJ_3504(iucA)
PAQPAGR_g3927
PSTWDSJ_03475
VCYIX92_17045
VCTJV59_21980
VMIAL543_14510
VSHBSZ05_19015
VKABTD91_15900
VFIVF_A0161(iucA)
VFMVFMJ11_A0197
GHOAL542_16550
PAGRE2H98_15790
PAEWKIH87_11620
SPOIIMCC21906_00381
MICTFIU95_16375(iucA)
MICZGL2_22460(iucA)
HTXEKK97_08795
KUSB9G99_09235
KMAB9H00_05615
KUYFY550_02635
PAURFGL86_03600
KAKKalk_08740
KKOKkor_2043
KGETQ33_0710
CHROCXB49_00480
CCROCMC5_023400(iucA)
MESPC1M53_02790
KMNHW532_05395
OANOant_3257
OAHDR92_3232
OCRHGK82_18845
OCLGTN27_19020
BVVBHK69_19680
LNEFZC33_08110
METM446_5062
METSDK389_06850
BBABd1576(iucA)
BBATBdt_1569(iucA)
BBACEP01_04100
BDZDOM22_13150
BSTOC0V70_12485
 » show all
Reference
1  [PMID:4313071]
  Authors
Gibson F, Magrath DI.
  Title
The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I.
  Journal
Biochim Biophys Acta 192:175-84 (1969)
DOI:10.1016/0304-4165(69)90353-5
Reference
2
  Authors
Maurer, P.J. and Miller, M.
  Title
Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine.
  Journal
J Am Chem Soc 104:3096-3101 (1982)
Reference
3  [PMID:2935523]
  Authors
de Lorenzo V, Bindereif A, Paw BH, Neilands JB
  Title
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
  Journal
J Bacteriol 165:570-8 (1986)
DOI:10.1128/JB.165.2.570-578.1986
  Sequence
Reference
4
  Authors
Appanna, D.L., Grundy, B.J., Szczepan, E.W. and Viswanatha, T.
  Title
Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1.
  Journal
Biochim Biophys Acta 801:437-443 (1984)
Reference
5  [PMID:15719346]
  Authors
Challis GL.
  Title
A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases.
  Journal
Chembiochem 6:601-11 (2005)
DOI:10.1002/cbic.200400283
Reference
6  [PMID:19865642]
  Authors
Oves-Costales D, Kadi N, Challis GL
  Title
The long-overlooked enzymology of a nonribosomal peptide synthetase-independent pathway for virulence-conferring siderophore biosynthesis.
  Journal
Chem Commun (Camb) 6530-41 (2009)
DOI:10.1039/b913092f
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.38
IUBMB Enzyme Nomenclature: 6.3.2.38
ExPASy - ENZYME nomenclature database: 6.3.2.38
BRENDA, the Enzyme Database: 6.3.2.38

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