KEGG   ENZYME: 6.3.2.39Help
Entry
EC 6.3.2.39                 Enzyme                                 

Name
aerobactin synthase;
iucC (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
N2-citryl-N6-acetyl-N6-hydroxy-L-lysine:N6-acetyl-N6-hydroxy-L-lysine ligase (ADP-forming)
Reaction(IUBMB)
2 ATP + N2-citryl-N6-acetyl-N6-hydroxy-L-lysine + N6-acetyl-N6-hydroxy-L-lysine + H2O = 2 ADP + 2 phosphate + aerobactin [RN:R10091]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
N2-citryl-N6-acetyl-N6-hydroxy-L-lysine [CPD:C20333];
N6-acetyl-N6-hydroxy-L-lysine [CPD:C03955];
H2O [CPD:C00001]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
aerobactin [CPD:C05554]
Comment
Requires Mg2+. Aerobactin is one of a group of high-affinity iron chelators known as siderophores and is produced under conditions of iron deprivation [6]. It is a dihydroxamate comprising two molecules of N6-acetyl-N6-hydroxy-L-lysine and one molecule of citric acid. This enzyme catalyses the second of two synthase reactions to link N6-acetyl-N6-hydroxy-L-lysine and citrate [3,4,5].
History
EC 6.3.2.39 created 2012
Pathway
ec00310  Lysine degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K03895  aerobactin synthase
Genes
EOJ: ECO26_1347(iucC)
EOI: ECO111_1295(iucC)
ECOO: ECRM13514_1292(iucC)
ECOH: ECRM13516_1248(iucC)
ESO: O3O_02280
ESM: O3M_22990
ESL: O3K_23075
ECC: c3625(iucC)
ECV: APECO1_O1CoBM170(iucC)
ECM: EcSMS35_3180(iucC)
ECQ: ECED1_4903(iucC)
ECK: EC55989_4643(iucC)
ECT: ECIAI39_3425(iucC)
EOC: CE10_3448(iucC)
EUM: ECUMN_3373(iucC)
ECZ: ECS88_p0011(iucC)
ELN: NRG857_30238(iucC)
EAB: ECABU_c32810(iutC)
ENA: ECNA114_4699(iuvC)
ELC: i14_3318(iucC)
ELD: i02_3318(iucC)
ECOJ: P423_16370
ECOS: EC958_3273(iucC)
SFL: SF3717(iucC)
SFX: S4054(iucC)
SFV: SFV_3852(iucC)
SFE: SFxv_4050
SFN: SFy_5296
SFS: SFyv_5370
SFT: NCTC1_04014(iucC)
SSN: SSON_3603(iucC)
SBO: SBO_4339(iucC)
SBC: SbBS512_E4632(iucC)
ENC: ECL_03318(iucC)
EEC: EcWSU1_02921(iucC)
ECLX: LI66_14270
ECLY: LI62_15615
ECLZ: LI64_13680
CTU: Ctu_1p01060(iucC)
KPU: KP1_p317(iucC)
KPR: KPR_4895(iucC)
KPNK: BN49_pII0066(iucC)
CKO: CKO_00971
YPE: YPO0992(iucC)
YPK: y3383(iucC)
YPH: YPC_0800(iucC)
YPA: YPA_0272
YPN: YPN_3190
YPM: YP_3448(iucC)
YPG: YpAngola_A0192(iucC)
YPZ: YPZ3_0746(iucC)
YPD: YPD4_0701(iucC)
YPX: YPD8_0701(iucC)
YPW: CH59_871
YPJ: CH55_3548
YPV: BZ15_2581
YPL: CH46_4153
YPS: YPTB3265(iucC)
YPO: BZ17_3345
YPI: YpsIP31758_0712(iucC)
YPY: YPK_0784
YPB: YPTS_3401
YPQ: DJ40_3172
YPU: BZ21_2545
YPR: BZ20_2849
YPC: BZ23_2826
YPF: BZ19_2671
YIN: CH53_2435
SPE: Spro_0920
SRL: SOD_c07760(iucC)
SPLY: Q5A_004130(iucC)
SMAF: D781_0885
PEC: W5S_0840
EGE: EM595_p0528(iucC)
PAM: PANA_0352(iucC)
PLF: PANA5342_4062(iucC)
PAJ: PAJ_3507(iucC)
PSTW: DSJ_03465
VFI: VF_A0163(iucC)
SWD: Swoo_2658
NOC: Noc_1812
NWA: Nwat_1300
KKO: Kkor_2041
KGE: TQ33_0708
CCRO: CMC5_023420(iucA)
BBA: Bd1574(iucC)
BBAT: Bdt_1567(iucC)
BBAC: EP01_04090
BMX: BMS_0526(iucC)
OAN: Oant_3255
OAH: DR92_3234
MET: M446_5060
 » show all
Taxonomy
Reference
1
  Authors
Appanna, D.L., Grundy, B.J., Szczepan, E.W. and Viswanatha, T.
  Title
Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1.
  Journal
Biochim Biophys Acta 801:437-443 (1984)
Reference
2  [PMID:4313071]
  Authors
Gibson F, Magrath DI.
  Title
The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I.
  Journal
Biochim Biophys Acta 192:175-84 (1969)
DOI:10.1016/0304-4165(69)90353-5
Reference
3
  Authors
Maurer, P.J. and Miller, M.
  Title
Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine.
  Journal
J Am Chem Soc 104:3096-3101 (1982)
Reference
4  [PMID:2935523]
  Authors
de Lorenzo V, Bindereif A, Paw BH, Neilands JB.
  Title
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
  Journal
J Bacteriol 165:570-8 (1986)
DOI:10.1128/JB.165.2.570-578.1986
  Sequence
Reference
5  [PMID:3087960]
  Authors
de Lorenzo V, Neilands JB
  Title
Characterization of iucA and iucC genes of the aerobactin system of plasmid ColV-K30 in Escherichia coli.
  Journal
J Bacteriol 167:350-5 (1986)
DOI:10.1128/JB.167.1.350-355.1986
  Sequence
Reference
6  [PMID:15719346]
  Authors
Challis GL.
  Title
A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases.
  Journal
Chembiochem 6:601-11 (2005)
DOI:10.1002/cbic.200400283
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.39
IUBMB Enzyme Nomenclature: 6.3.2.39
ExPASy - ENZYME nomenclature database: 6.3.2.39
BRENDA, the Enzyme Database: 6.3.2.39

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