iucC (gene name)
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
N2-citryl-N6-acetyl-N6-hydroxy-L-lysine:N6-acetyl-N6-hydroxy-L-lysine ligase (AMP-forming)
ATP + N2-citryl-N6-acetyl-N6-hydroxy-L-lysine + N6-acetyl-N6-hydroxy-L-lysine = AMP + diphosphate + aerobactin [RN:
Requires Mg2+. The enzyme is involved in the biosynthesis of aerobactin, a dihydroxamate siderophore. It belongs to a class of siderophore synthases known as type C nonribosomal peptide synthase-independent synthases (NIS). Type C enzymes are responsible for the formation of amide or ester bonds between a variety of substrates and a prochiral carboxyl group of a citrate molecule that is already linked to a different moiety at its other prochiral carboxyl group. The enzyme is believed to form an adenylate intermediate prior to ligation.
EC 220.127.116.11 created 2012, modified 2019
ec00997 Biosynthesis of various other secondary metabolites
ec01110 Biosynthesis of secondary metabolites
ec01120 Microbial metabolism in diverse environments
SOF: NCTC11214_00580(iucC) » show all
Gibson F, Magrath DI.
The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I.
Maurer, P.J. and Miller, M.
Microbial iron chelators: total synthesis of aerobactin and its constituent amino acid, N6-acetyl-N6-hydroxylysine.
J Am Chem Soc 104:3096-3101 (1982)
Appanna, D.L., Grundy, B.J., Szczepan, E.W. and Viswanatha, T.
Aerobactin synthesis in a cell-free system of Aerobacter aerogenes 62-1.
Biochim Biophys Acta 801:437-443 (1984)
de Lorenzo V, Bindereif A, Paw BH, Neilands JB
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
de Lorenzo V, Neilands JB
Characterization of iucA and iucC genes of the aerobactin system of plasmid ColV-K30 in Escherichia coli.
A widely distributed bacterial pathway for siderophore biosynthesis independent of nonribosomal peptide synthetases.
Oves-Costales D, Kadi N, Challis GL
The long-overlooked enzymology of a nonribosomal peptide synthetase-independent pathway for virulence-conferring siderophore biosynthesis.
ExplorEnz - The Enzyme Database: 18.104.22.168
ExPASy - ENZYME nomenclature database: 22.214.171.124