KEGG   ENZYME: 6.3.2.43Help
Entry
EC 6.3.2.43                 Enzyme                                 

Name
[lysine-biosynthesis-protein LysW]---L-2-aminoadipate ligase;
alpha-aminoadipate-lysW ligase lysX (gene name);
LysX (ambiguous);
AAA---LysW ligase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
[lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate:L-2-aminoadipate ligase (ADP-forming)
Reaction(IUBMB)
ATP + [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate + L-2-aminoadipate = ADP + phosphate + [lysine-biosynthesis-protein LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate [RN:R09775]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
[lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate [CPD:C19885];
L-2-aminoadipate [CPD:C00956]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
[lysine-biosynthesis-protein LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate [CPD:C19886]
Comment
The enzymes from the thermophilic bacterium Thermus thermophilus and the thermophilic archaea Sulfolobus acidocaldarius and Sulfolobus tokodaii protect the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW. This reaction is part of the lysine biosynthesis pathway in these organisms.
History
EC 6.3.2.43 created 2014
Pathway
ec00300  Lysine biosynthesis
ec01100  Metabolic pathways
Orthology
K05827  [lysine-biosynthesis-protein LysW]---L-2-aminoadipate ligase
Genes
ENF: AKI40_3663(rimK)
SERA: Ser39006_009290
MSD: MYSTI_01368
CCX: COCOR_02065(lysX)
SAUC: CA347_99
PMS: KNP414_04939
PMQ: PM3016_4341
PMW: B2K_22490
PIH: UB51_14610
PSWU: SY83_08915
SGR: SGR_3478
SFA: Sfla_0121
STRP: F750_6903(lysX)
SPRI: SPRI_3646
SRW: TUE45_06896(lysX_3)
SLAU: SLA_3045
SALJ: SMD11_6708(lysX)
SESP: BN6_56710
ASE: ACPL_7493(rimK)
ACTS: ACWT_7363
CAI: Caci_6289
RCA: Rcas_3469
CAU: Caur_0334
CAG: Cagg_3597
HAU: Haur_3306
TRO: trd_1519(lysX) trd_A0165(lysX)
ATM: ANT_08250(lysX) ANT_12410(lysX)
CAP: CLDAP_04850(lysX)
DRA: DR_2194
DGE: Dgeo_1151
TRA: Trad_1392
TTH: TT_C1543
TTJ: TTHA1907
TSC: TSC_c01920(lysX)
TAQ: TO73_0098
MRB: Mrub_2724
TAI: Taci_0444
TLI: Tlie_1469
MRO: MROS_0165
CABY: Cabys_3148
HHB: Hhub_3648(argX)
HALH: HTSR_2057(lysX)
HHSR: HSR6_2133(lysX)
HSU: HLASF_2137(argA)
HSF: HLASA_2171(argA)
HMA: rrnAC2679(rimK)
HHI: HAH_3083(argX)
NPH: NP_5258A(argX)
NMO: Nmlp_1243(argX)
HUT: Huta_1502
HTI: HTIA_1539
HMU: Hmuk_0772
HARC: HARCEL1_01525(lysX)
HWA: HQ_3714A(argX)
HWC: Hqrw_4129(argX)
HVO: HVO_0046(argX)
HME: HFX_0047(argX)
HLA: Hlac_2616
SRUB: C2R22_20780(lysX)
HTU: Htur_0328
NMG: Nmag_1761(argX)
NAT: NJ7G_0263(argX)
SALI: L593_07885
PTO: PTO1469
FAI: FAD_0508(lysX)
CDIV: CPM_0545
PHO: PH1721(PH1721)
PAB: PAB0290(rimK)
TKO: TK0278
THS: TES1_0507
APE: APE_1463(lysX)
ACJ: ACAM_0923(lysX)
IHO: Igni_0057
IIS: EYM_02245
IAG: Igag_1752
SSO: SSO0159(rimK-1)
SOL: Ssol_1137
SSOA: SULA_1173
SSOL: SULB_1174
SSOF: SULC_1172
STO: STK_01920(lysX)
SAI: Saci_0754
SID: M164_1986
SII: LD85_2241
SIH: SiH_1924
SIR: SiRe_1851
SIC: SiL_1833
MSE: Msed_0168
MCN: Mcup_1905
AHO: Ahos_0887
PAI: PAE1240
PIS: Pisl_1291
PCL: Pcal_1379
PAS: Pars_0291
PYR: P186_2608
POG: Pogu_2164
TNE: Tneu_0259
CMA: Cmaq_1298
TUZ: TUZN_0807
TTN: TTX_0466(lysX_rimK)
VDI: Vdis_0200
VMO: VMUT_1538
NCT: NMSP_0393(lysX_1) NMSP_0403(lysX_2)
NGA: Ngar_c14560(lysX1) Ngar_c29970(lysX2)
NVN: NVIE_002660(lysX1) NVIE_016500(lysX2)
TAA: NMY3_02221(lysX_1) NMY3_02228(lysX_2)
NCV: NCAV_1252(lysX) NCAV_1409(lysX)
NBV: T478_1043(lysX) T478_1050(lysX)
NDV: NDEV_1623(lysX) NDEV_1630(lysX)
KCR: Kcr_0812
BARB: AOA66_0019(lysX)
 » show all
Taxonomy
Reference
1  [PMID:12925802]
  Authors
Vassylyeva MN, Sakai H, Matsuura T, Sekine S, Nishiyama M, Terada T, Shirouzu M, Kuramitsu S, Vassylyev DG, Yokoyama S
  Title
Cloning, expression, purification, crystallization and initial crystallographic analysis of the lysine-biosynthesis LysX protein from Thermus thermophilus HB8.
  Journal
Acta Crystallogr D Biol Crystallogr 59:1651-2 (2003)
Reference
2  [PMID:19620981]
  Authors
Horie A, Tomita T, Saiki A, Kono H, Taka H, Mineki R, Fujimura T, Nishiyama C, Kuzuyama T, Nishiyama M.
  Title
Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus.
  Journal
Nat Chem Biol 5:673-9 (2009)
DOI:10.1038/nchembio.198
  Sequence
[ttj:TTHA1907]
Reference
3  [PMID:23434852]
  Authors
Ouchi T, Tomita T, Horie A, Yoshida A, Takahashi K, Nishida H, Lassak K, Taka H, Mineki R, Fujimura T, Kosono S, Nishiyama C, Masui R, Kuramitsu S, Albers SV, Kuzuyama T, Nishiyama M
  Title
Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus.
  Journal
Nat Chem Biol 9:277-83 (2013)
DOI:10.1038/nchembio.1200
  Sequence
[sai:Saci_0754]
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.43
IUBMB Enzyme Nomenclature: 6.3.2.43
ExPASy - ENZYME nomenclature database: 6.3.2.43
BRENDA, the Enzyme Database: 6.3.2.43

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