KEGG ENZYME: 6.3.2.43

ENZYME: 6.3.2.43

Entry

EC 6.3.2.43                 Enzyme

Name

[amino-group carrier protein]---L-2-aminoadipate ligase;
alpha-aminoadipate-lysW ligase;
lysX (gene name);
LysX (ambiguous);
AAA---LysW ligase;
[lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate:L-2-aminoadipate ligase (ADP-forming);
[lysine-biosynthesis-protein LysW]---L-2-aminoadipate ligase

Class

Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)

Sysname

[amino-group carrier protein]-C-terminal-L-glutamate:L-2-aminoadipate ligase (ADP-forming)

Reaction(IUBMB)

ATP + an [amino-group carrier protein]-C-terminal-L-glutamate + L-2-aminoadipate = ADP + phosphate + an [amino-group carrier protein]-C-terminal-[N-(1,4-dicarboxybutyl)-L-glutamine]

Reaction(KEGG)

(other) R09775

Substrate

ATP [CPD:C00002];
[amino-group carrier protein]-C-terminal-L-glutamate;
L-2-aminoadipate [CPD:C00956]

Product

ADP [CPD:C00008];
phosphate [CPD:C00009];
[amino-group carrier protein]-C-terminal-[N-(1,4-dicarboxybutyl)-L-glutamine]

Comment

The enzymes from the thermophilic bacterium Thermus thermophilus and the thermophilic archaea Sulfolobus acidocaldarius and Sulfolobus tokodaii protect the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW. This reaction is part of the lysine biosynthesis pathway in these organisms.

History

EC 6.3.2.43 created 2014, modified 2019

Pathway

ec00300

Lysine biosynthesis

ec01100

Metabolic pathways

Orthology

K05827

[lysine-biosynthesis-protein LysW]---L-2-aminoadipate ligase

K25032

alpha-aminoadipate-LysW ligase/glutamate-LysW ligase

Genes

ENF:	AKI40_3663(rimK)

SERA:

Ser39006_009290

SERQ:

CWC46_09285

CARI:

FNU76_03420

MSD:	MYSTI_01368

MFB:	MFUL124B02_07595

MLAN:

JY572_19520

CCX:	COCOR_02065(lysX)

PPAK:

JY651_31930

AVM:	JQX13_36445

GAJ:	MY490_21305

SAUC:

CA347_99

PMS:	KNP414_04939

PMQ:	PM3016_4341

PMW:

B2K_22490

PIH:	UB51_14610

PSWU:

SY83_08915

PWN:	QNH46_08345

ATHE:

K3F53_07355

ALIC:

GI364_20565

SJT:	PGH26_08345

GEK:	kuro4_23340

SGR:

SGR_3478

SFI:

SFUL_3350

SSX:	SACTE_1737

SFA:

Sfla_0121

STRP:

F750_6903(lysX)

SCI:	B446_19865

SGU:	SGLAU_17270

SLC:	SL103_03060

SPRI:

SPRI_3646

SCZ:	ABE83_19110

SCX:	AS200_22005

SRW:	TUE45_06896(lysX_3)

SPAV:

Spa2297_32325

STRT:

A8713_31065

SALW:

CP975_34120

SLAU:

SLA_3045

SALJ:

SMD11_6708(lysX)

STRO:

STRMOE7_34030

STIR:

DDW44_16565

SVN:	CP980_30520

SNK:	CP967_01830 CP967_15205

SFIC:

EIZ62_06125

SSPO:

DDQ41_08075

SCAV:

CVT27_15440

SCYG:

S1361_31450(lysX4)

SGJ:	IAG43_00720

STRY:

EQG64_16285

SDD:	D9753_06830 D9753_22730

SINE:

KI385_33350

SDEC:

L3078_16725

SAKB:

K1J60_19050 K1J60_28145

SCAE:

IHE65_28585 IHE65_43745

SSPN:

LXH13_29730

SOV:	QZH56_15290

SROC:

RGF97_02730 RGF97_22075

SLUO:

PS467_10000 PS467_34275

STPB:

QR97_19335

SCAJ:

O1G22_44210

KAB:	B7C62_16025

KIT:	CFP65_3950

AGRA:

AGRA3207_003394

ACIR:

OG979_26760(lysX)

MOM:	KUM42_00905

SESP:

BN6_56710

SSYI:

EKG83_30610(lysX)

MENO:

Jiend_51340

MFEU:

H1D33_25920

ASE:	ACPL_7493(rimK)

ACTS:

ACWT_7363

ACTR:

Asp14428_21600

PLK:	CIK06_18345

DAUR:

Daura_16095(lysX)

CAI:

Caci_6289

NAV:	JQS30_10100

GLY:	K3N28_10255

RRS:	RoseRS_1059

RCA:

Rcas_3469

CAU:

Caur_0334

CHL:	Chy400_0359

CAG:

Cagg_3597

HAU:

Haur_3306

CALL:

OZ401_000132(lysX) OZ401_003951

ATM:	ANT_08250(lysX) ANT_12410(lysX)

PSUB:

Pelsub_P1480 Pelsub_P2050

LEK:	hrd7_27800 hrd7_27890

CAP:	CLDAP_04850(lysX)

KBS:	EPA93_38760(lysX)

KBB:	ccbrp13_35990

THUG:

KNN16_06510(lysX)

TTR:	Tter_0316 Tter_0318

PMET:

G4Y79_18395(lysX)

TRO:	trd_1519(lysX) trd_A0165(lysX)

STI:	Sthe_0213 Sthe_0215 Sthe_2943

DRA:

DR_2194

DGE:

Dgeo_1151

DDR:	Deide_13430

DMR:	Deima_1350

DGO:	DGo_CA1538

DPD:	Deipe_2759

DSW:	QR90_07825

DCH:	SY84_02035

DAB:	AUC44_07740

DPU:	SU48_08690

DEZ:	DKM44_08535(lysX)

DWU:	DVJ83_00355(lysX)

DFC:

DFI_07055

DEIN:

DAAJ005_09940(lysX)

DGA:	DEGR_14210

DPH:	EHF33_03465(lysX)

DRB:	N0D28_11275(lysX)

DAH:	DAETH_14790

DAQU:

M8445_12315(lysX)

DTAE:

LAJ19_06370(lysX)

DMB:	E5F05_13845(lysX)

TRA:

Trad_1392

TTH:

TT_C1543

TTJ:	TTHA1907(TTHA1907)

TTS:	Ththe16_1907

TTL:	TtJL18_1989

TSC:	TSC_c01920(lysX)

THC:	TCCBUS3UF1_21030

TOS:	Theos_1933

TAQ:

TO73_0098

TBC:	A0O31_00870

TANT:

KNN15_05130(lysX)

MRB:

Mrub_2724

MRE:	K649_08915

MTAI:

Mtai_v1c23230

MSV:	Mesil_0438

OPR:	Ocepr_1784

MHD:	Marky_0668

UAM:	UABAM_02298

SCOR:

J3U87_02875(lysX)

TAI:

Taci_0444

CPOR:

BED41_13050

TLI:

Tlie_1469

AMO:	Anamo_0990

ARAM:

KAR29_00420(lysX)

MRO:

MROS_0165

CABY:

Cabys_3148

PFU:	PF0209 PF1682

PFI:	PFC_00105 PFC_10195

PHO:	PH1721(PH1721)

PAB:	PAB0290(rimK)

PYN:	PNA2_0329 PNA2_0499

PYS:	Py04_0364 Py04_1636

TKO:

TK0278

THS:

TES1_0507

THV:	ADU37_CDS10050

TCH:	CHITON_0428 CHITON_1979

THEI:

K1720_00615(lysX)

PTO:

PTO1469

FAC:	FACI_IFERC01G0468 FACI_IFERC01G1527

FAI:	FAD_0508(lysX)

CDIV:

CPM_0545

APE:	APE_1463(lysX)

ACJ:	ACAM_0923(lysX)

IHO:

Igni_0057

IIS:

EYM_02245

IPC:

IPA_00760

IAG:

Igag_1752

PFM:	Pyrfu_0740

PDL:	Pyrde_1837

PABI:

PABY_17850(lysX_2)

PARE:

PYJP_07930(lysX_2)

STO:	STK_01920(lysX)

SOH:	D1869_00430(lysX)

SSO:	SSO0159(rimK-1)

SOL:

Ssol_1137

SSOA:

SULA_1173(lysX)

SSOL:

SULB_1174(lysX)

SSOF:

SULC_1172(lysX)

SSHI:

J5U23_00105

SCAS:

SACC_00720

SAI:

Saci_0754

SACN:

SacN8_03665

SACR:

SacRon12I_03650

SACS:

SUSAZ_03380

SIS:	LS215_2145

SIA:	M1425_1980

SIM:	M1627_2058

SID:

M164_1986

SIY:	YG5714_2103

SIN:	YN1551_0815

SII:

LD85_2241

SIH:

SiH_1924

SIR:

SiRe_1851

SIC:

SiL_1833

SULA:

BFU36_05140

SULE:

GFS03_10845(lysX)

SULO:

GFS33_02660(lysX)

SULL:

EWF20_01870(lysX)

MSE:

Msed_0168

MEMJ:

MJ1HA_0247

MCN:

Mcup_1905

MHK:	DFR87_05970(lysX)

MPRU:

DFR88_05800(lysX)

MTEN:

GWK48_06250(lysX)

MJN:	MjAS7_0751

AHO:

Ahos_0887

AMAN:

B6F84_08285

ABRI:

DFR85_06560(lysX)

ASUL:

DFR86_07300(lysX)

AAMB:

D1866_12330(lysX)

ACIH:

HS5_22620

SACD:

HS1genome_0848

SAZO:

D1868_01575(lysX)

CSTY:

KN1_18130

STEP:

IC006_1906

SMET:

RQ359_002317(lysX)

PAI:

PAE1240

PIS:

Pisl_1291

PCL:

Pcal_1379

PAS:

Pars_0291

PYR:

P186_2608

POG:

Pogu_2164

TNE:

Tneu_0259

PYW:	PYWP30_00341

CMA:

Cmaq_1298

TTN:	TTX_0466(lysX_rimK)

TUZ:

TUZN_0807

VDI:

Vdis_0200

VMO:

VMUT_1538

VSO:	Vsou_14220

KCR:

Kcr_0812

NMR:	Nmar_1288 Nmar_1295

NIR:	NSED_07150 NSED_07185

NKR:	NKOR_07270 NKOR_07310

NID:	NPIRD3C_0829(lysX) NPIRD3C_0836

NIN:	NADRNF5_0661(lysX) NADRNF5_0668

NIW:	Nisw_07255(lysX) Nisw_07290(lysX)

NCL:	C5F47_06940(lysX) C5F47_06975(lysX)

NOX:	C5F49_06950(lysX) C5F49_06985(lysX)

NUE:	C5F50_09705(lysX) C5F50_09740(lysX)

NIP:	NsoK4_01565(lysX) NsoK4_01595(lysX)

NZT:	NZOSNM25_000516 NZOSNM25_000523

NCT:	NMSP_0393(lysX_1) NMSP_0403(lysX_2)

NIC:	DSQ20_07905(lysX) DSQ20_07940(lysX)

NGA:	Ngar_c14560(lysX1) Ngar_c29970(lysX2)

NVN:	NVIE_002660(lysX1) NVIE_016500(lysX2)

NEV:	NTE_00733 NTE_01582

TAA:	NMY3_02221(lysX_1) NMY3_02228(lysX_2)

NFN:	NFRAN_2983(lysX) NFRAN_2990(lysX)

NCV:	NCAV_1252(lysX) NCAV_1409(lysX)

CSY:	CENSYa_0124 CENSYa_0927

CSU:	CSUB_C1306

NBV:	T478_1043(lysX) T478_1050(lysX)

TAH:	SU86_009045 SU86_009085

NIU:	DSQ19_03190(lysX) DSQ19_03225(lysX)

NCK:	QVH35_07210

NDV:	NDEV_1623(lysX) NDEV_1630(lysX)

CCAI:

NAS2_0266 NAS2_0272

» show all

Reference

1 [PMID:12925802]

Authors

Vassylyeva MN, Sakai H, Matsuura T, Sekine S, Nishiyama M, Terada T, Shirouzu M, Kuramitsu S, Vassylyev DG, Yokoyama S

Title

Cloning, expression, purification, crystallization and initial crystallographic analysis of the lysine-biosynthesis LysX protein from Thermus thermophilus HB8.

Journal

Acta Crystallogr D Biol Crystallogr 59:1651-2 (2003)
DOI:10.1107/S0907444903014720

Reference

2 [PMID:19620981]

Authors

Horie A, Tomita T, Saiki A, Kono H, Taka H, Mineki R, Fujimura T, Nishiyama C, Kuzuyama T, Nishiyama M

Title

Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus.

Journal

Nat Chem Biol 5:673-9 (2009)
DOI:10.1038/nchembio.198

Sequence

[ttj:TTHA1907]

Reference

3 [PMID:23434852]

Authors

Ouchi T, Tomita T, Horie A, Yoshida A, Takahashi K, Nishida H, Lassak K, Taka H, Mineki R, Fujimura T, Kosono S, Nishiyama C, Masui R, Kuramitsu S, Albers SV, Kuzuyama T, Nishiyama M

Title

Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus.

Journal

Nat Chem Biol 9:277-83 (2013)
DOI:10.1038/nchembio.1200

Sequence

[sai:Saci_0754]

Other DBs

ExplorEnz - The Enzyme Database:

6.3.2.43

IUBMB Enzyme Nomenclature:

6.3.2.43

ExPASy - ENZYME nomenclature database:

6.3.2.43

BRENDA, the Enzyme Database:

6.3.2.43

DBGET integrated database retrieval system