KEGG   ENZYME: 6.3.2.43
Entry
EC 6.3.2.43                 Enzyme                                 
Name
[amino-group carrier protein]---L-2-aminoadipate ligase;
alpha-aminoadipate-lysW ligase;
lysX (gene name);
LysX (ambiguous);
AAA---LysW ligase;
[lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate:L-2-aminoadipate ligase (ADP-forming);
[lysine-biosynthesis-protein LysW]---L-2-aminoadipate ligase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
[amino-group carrier protein]-C-terminal-L-glutamate:L-2-aminoadipate ligase (ADP-forming)
Reaction(IUBMB)
ATP + an [amino-group carrier protein]-C-terminal-L-glutamate + L-2-aminoadipate = ADP + phosphate + an [amino-group carrier protein]-C-terminal-[N-(1,4-dicarboxybutyl)-L-glutamine]
Reaction(KEGG)
(other) R09775
Substrate
ATP [CPD:C00002];
[amino-group carrier protein]-C-terminal-L-glutamate;
L-2-aminoadipate [CPD:C00956]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
[amino-group carrier protein]-C-terminal-[N-(1,4-dicarboxybutyl)-L-glutamine]
Comment
The enzymes from the thermophilic bacterium Thermus thermophilus and the thermophilic archaea Sulfolobus acidocaldarius and Sulfolobus tokodaii protect the amino group of L-2-aminoadipate by conjugation to the carrier protein LysW. This reaction is part of the lysine biosynthesis pathway in these organisms.
History
EC 6.3.2.43 created 2014, modified 2019
Pathway
ec00300  Lysine biosynthesis
ec01100  Metabolic pathways
Orthology
K05827  [lysine-biosynthesis-protein LysW]---L-2-aminoadipate ligase
K25032  alpha-aminoadipate-LysW ligase/glutamate-LysW ligase
Genes
ENFAKI40_3663(rimK)
SERASer39006_009290
SERQCWC46_09285
CARIFNU76_03420
MSDMYSTI_01368
MFBMFUL124B02_07595
CCXCOCOR_02065(lysX)
AVMJQX13_36445
GAJMY490_21305
SAUCCA347_99
PMSKNP414_04939
PMQPM3016_4341
PMWB2K_22490
PIHUB51_14610
PSWUSY83_08915
ATHEK3F53_07355
GEKkuro4_23340
SGRSGR_3478
SSXSACTE_1737
SFASfla_0121
STRPF750_6903(lysX)
SFISFUL_3350
SCIB446_19865
SGUSGLAU_17270
SLCSL103_03060
SPRISPRI_3646
SCZABE83_19110
SCXAS200_22005
SRWTUE45_06896(lysX_3)
SPAVSpa2297_32325
STRTA8713_31065
SALWCP975_34120
SLAUSLA_3045
SALJSMD11_6708(lysX)
STROSTRMOE7_34030
STIRDDW44_16565
SVNCP980_30520
SNKCP967_01830 CP967_15205
SFICEIZ62_06125
SSPODDQ41_08075
SCAVCVT27_15440
SCYGS1361_31450(lysX4)
SGJIAG43_00720
STRYEQG64_16285
SDDD9753_06830 D9753_22730
SINEKI385_33350
SDECL3078_16725
KABB7C62_16025
KITCFP65_3950
AGRAAGRA3207_003394
SESPBN6_56710
SSYIEKG83_30610(lysX)
ASEACPL_7493(rimK)
ACTSACWT_7363
ACTRAsp14428_21600
PLKCIK06_18345
CAICaci_6289
NAVJQS30_10100
GLYK3N28_10255
RRSRoseRS_1059
RCARcas_3469
CAUCaur_0334
CHLChy400_0359
CAGCagg_3597
HAUHaur_3306
TROtrd_1519(lysX) trd_A0165(lysX)
STISthe_0213 Sthe_0215 Sthe_2943
ATMANT_08250(lysX) ANT_12410(lysX)
PSUBPelsub_P1480 Pelsub_P2050
LEKhrd7_27800 hrd7_27890
CAPCLDAP_04850(lysX)
KBSEPA93_38760(lysX)
THUGKNN16_06510(lysX)
TTRTter_0316 Tter_0318
PMETG4Y79_18395(lysX)
DRADR_2194
DGEDgeo_1151
DDRDeide_13430
DMRDeima_1350
DGODGo_CA1538
DPDDeipe_2759
DSWQR90_07825
DCHSY84_02035
DABAUC44_07740
DPUSU48_08690
DEZDKM44_08535(lysX)
DWUDVJ83_00355(lysX)
DFCDFI_07055
DEINDAAJ005_09940(lysX)
DGADEGR_14210
DPHEHF33_03465(lysX)
TRATrad_1392
TTHTT_C1543
TTJTTHA1907(TTHA1907)
TTSThthe16_1907
TTLTtJL18_1989
TSCTSC_c01920(lysX)
THCTCCBUS3UF1_21030
TOSTheos_1933
TAQTO73_0098
TBCA0O31_00870
TANTKNN15_05130(lysX)
MRBMrub_2724
MREK649_08915
MSVMesil_0438
MTAIMtai_v1c23230
OPROcepr_1784
MHDMarky_0668
UAMUABAM_02298
SCORJ3U87_02875(lysX)
TAITaci_0444
TLITlie_1469
AMOAnamo_0990
CPORBED41_13050
MROMROS_0165
CABYCabys_3148
PFUPF0209 PF1682
PFIPFC_00105 PFC_10195
PHOPH1721(PH1721)
PABPAB0290(rimK)
PYNPNA2_0329 PNA2_0499
PYSPy04_0364 Py04_1636
TKOTK0278
THSTES1_0507
THVADU37_CDS10050
TCHCHITON_0428 CHITON_1979
THEIK1720_00615(lysX)
HDLHALDL1_01655
HHBHhub_3648(argX)
HNOLT974_00080(lysX)
HLULT972_14180(lysX)
HJEHacjB3_00990
HALHHTSR_2057(lysX)
HHSRHSR6_2133(lysX)
HSUHLASF_2137(argA)
HSFHLASA_2171(argA)
SALRFQU85_04735(lysX)
HALREFA46_004295(lysX) EFA46_005465(lysX)
HARAAArcS_3061(rimK3)
HAADMW046_06605(lysX)
HALXM0R89_05925(lysX)
HAXZM0R88_06110(lysX)
HMArrnAC2679(rimK)
HHIHAH_3083(argX)
HHNHISP_15695
HABSG26_07790
HTABVU17_15005
HALJG9465_02505(lysX)
HSINKDQ40_13795
NPHNP_5258A(argX)
NMONmlp_1243(argX)
NHOHWV23_08510(lysX)
NSALHWV07_10855(lysX)
HUTHuta_1502
HTIHTIA_1539
HALAHrd1104_12295(lysX)
HMUHmuk_0772
HALZE5139_07045
HALLLC1Hm_3288(lysX)
HAZPGBQ70_07040
HALIBV210_07075
HSNDV733_03395(lysX)
HDSHSR122_1705(rimK2)
HRRHZS55_04375(lysX)
HPELHZS54_04325(lysX)
HLTI7X12_08365(lysX)
HARCHARCEL1_01525(lysX)
HDOMUK72_02365(lysX)
HWAHQ_3714A(argX)
HWCHqrw_4129(argX)
HVOHVO_0046(argX)
HMEHFX_0047(argX)
HGIABY42_00220
HALEG3A49_00610(lysX)
HBOHbor_01730
HAQDU484_03085(lysX)
HAJDU500_03525(lysX)
HAERDU502_01270(lysX)
HRAEI982_12725(lysX)
HLMDV707_00740(lysX)
HALMFCF25_06925(lysX)
HLAHlac_2616
HALPDOS48_13135(lysX)
HALBEKH57_10855
HEZZEO776_15095(lysX)
HALQHrr1229_007375(lysX)
HSSJ7656_01825(lysX)
HSAIHPS36_07360(lysX)
HAZZKI388_13080(lysX)
SRUBC2R22_20780(lysX)
HAEhalTADL_1873
HALNB4589_001575(lysX)
HALGHUG10_12795
HALUHUG12_02040(lysX)
HMPK6T50_10115(lysX)
HREK6T36_09880(lysX)
HRMK6T25_06300(lysX)
HACBHbl1158_01725(lysX)
HDFAArcSl_0043(lysX)
HAHHalar_1202
HTUHtur_0328
HDABB347_12550
HSALJMJ58_07535(lysX)
HLOJ0X27_11130(lysX)
HAKZJ0X25_08135(lysX)
NMGNmag_1761(argX)
HXAHalxa_1378
NATNJ7G_0263(argX)
NPENatpe_3686
NVRFEJ81_17605(lysX)
NPLFGF80_16430(lysX)
NAYHYG81_11420(lysX)
HALYHYG82_14935(lysX)
HJTDVR14_08510(lysX)
NGENatgr_1612
HRUHalru_0399
NOUNatoc_0148
SALIL593_07885
SAIMK0C01_05795(lysX)
HLRHALLA_05490
HLCCHINAEXTREME04215
NAJB1756_08250
NAGAArcMg_3554
NANAArc1_3498
NBGDV706_07690(lysX)
NASGCU68_05005(lysX)
SAWLNGM29_05185(lysX)
PTOPTO1469
FACFACI_IFERC01G0468 FACI_IFERC01G1527
FAIFAD_0508(lysX)
CDIVCPM_0545
APEAPE_1463(lysX)
ACJACAM_0923(lysX)
IHOIgni_0057
IISEYM_02245
IAGIgag_1752
PFMPyrfu_0740
PDLPyrde_1837
STOSTK_01920(lysX)
SOHD1869_00430(lysX)
SSOSSO0159(rimK-1)
SOLSsol_1137
SSOASULA_1173(lysX)
SSOLSULB_1174(lysX)
SSOFSULC_1172(lysX)
SSHIJ5U23_00105
SCASSACC_00720
SAISaci_0754
SACNSacN8_03665
SACRSacRon12I_03650
SACSSUSAZ_03380
SISLS215_2145
SIAM1425_1980
SIMM1627_2058
SIDM164_1986
SIYYG5714_2103
SINYN1551_0815
SIILD85_2241
SIHSiH_1924
SIRSiRe_1851
SICSiL_1833
SULABFU36_05140
SULEGFS03_10845(lysX)
SULOGFS33_02660(lysX)
SULLEWF20_01870(lysX)
MSEMsed_0168
MCNMcup_1905
MHKDFR87_05970(lysX)
MPRUDFR88_05800(lysX)
MTENGWK48_06250(lysX)
MJNMjAS7_0751
MEMJMJ1HA_0247
AHOAhos_0887
AMANB6F84_08285
ABRIDFR85_06560(lysX)
ASULDFR86_07300(lysX)
AAMBD1866_12330(lysX)
ACIHHS5_22620
SACDHS1genome_0848
SAZOD1868_01575(lysX)
CSTYKN1_18130
STEPIC006_1906
PAIPAE1240
PISPisl_1291
PCLPcal_1379
PASPars_0291
PYRP186_2608
POGPogu_2164
TNETneu_0259
PYWPYWP30_00341
CMACmaq_1298
TTNTTX_0466(lysX_rimK)
TUZTUZN_0807
VDIVdis_0200
VMOVMUT_1538
NMRNmar_1288 Nmar_1295
NIRNSED_07150 NSED_07185
NKRNKOR_07270 NKOR_07310
NIDNPIRD3C_0829(lysX) NPIRD3C_0836
NINNADRNF5_0661(lysX) NADRNF5_0668
NIWNisw_07255(lysX) Nisw_07290(lysX)
NCLC5F47_06940(lysX) C5F47_06975(lysX)
NOXC5F49_06950(lysX) C5F49_06985(lysX)
NUEC5F50_09705(lysX) C5F50_09740(lysX)
NIPNsoK4_01565(lysX) NsoK4_01595(lysX)
NCTNMSP_0393(lysX_1) NMSP_0403(lysX_2)
NICDSQ20_07905(lysX) DSQ20_07940(lysX)
CSYCENSYa_0124 CENSYa_0927
NGANgar_c14560(lysX1) Ngar_c29970(lysX2)
NVNNVIE_002660(lysX1) NVIE_016500(lysX2)
NEVNTE_00733 NTE_01582
TAANMY3_02221(lysX_1) NMY3_02228(lysX_2)
NFNNFRAN_2983(lysX) NFRAN_2990(lysX)
NCVNCAV_1252(lysX) NCAV_1409(lysX)
CSUCSUB_C1306
NBVT478_1043(lysX) T478_1050(lysX)
TAHSU86_009045 SU86_009085
NIUDSQ19_03190(lysX) DSQ19_03225(lysX)
NDVNDEV_1623(lysX) NDEV_1630(lysX)
CCAINAS2_0266 NAS2_0272
KCRKcr_0812
 » show all
Reference
1  [PMID:12925802]
  Authors
Vassylyeva MN, Sakai H, Matsuura T, Sekine S, Nishiyama M, Terada T, Shirouzu M, Kuramitsu S, Vassylyev DG, Yokoyama S
  Title
Cloning, expression, purification, crystallization and initial crystallographic analysis of the lysine-biosynthesis LysX protein from Thermus thermophilus HB8.
  Journal
Acta Crystallogr D Biol Crystallogr 59:1651-2 (2003)
DOI:10.1107/S0907444903014720
Reference
2  [PMID:19620981]
  Authors
Horie A, Tomita T, Saiki A, Kono H, Taka H, Mineki R, Fujimura T, Nishiyama C, Kuzuyama T, Nishiyama M
  Title
Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus.
  Journal
Nat Chem Biol 5:673-9 (2009)
DOI:10.1038/nchembio.198
  Sequence
[ttj:TTHA1907]
Reference
3  [PMID:23434852]
  Authors
Ouchi T, Tomita T, Horie A, Yoshida A, Takahashi K, Nishida H, Lassak K, Taka H, Mineki R, Fujimura T, Kosono S, Nishiyama C, Masui R, Kuramitsu S, Albers SV, Kuzuyama T, Nishiyama M
  Title
Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus.
  Journal
Nat Chem Biol 9:277-83 (2013)
DOI:10.1038/nchembio.1200
  Sequence
[sai:Saci_0754]
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.43
IUBMB Enzyme Nomenclature: 6.3.2.43
ExPASy - ENZYME nomenclature database: 6.3.2.43
BRENDA, the Enzyme Database: 6.3.2.43

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