Entry
Name
UDP-N-acetylmuramate---L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate ligase;
murein peptide ligase;
Mpl;
yjfG (gene name);
UDP-MurNAc:L-Ala-gamma-D-Glu-meso-A2pm ligase;
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate ligase2015
Reaction(IUBMB)
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate [RN:
R10901 ]
Reaction(KEGG)
Substrate
ATP [CPD:
C00002 ];
UDP-N-acetyl-alpha-D-muramate [CPD:
C01050 ];
L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate [CPD:
C20925 ]
Product
ADP [CPD:
C00008 ];
phosphate [CPD:
C00009 ];
UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate [CPD:
C04877 ]
Comment
The enzyme catalyses the reincorporation into peptidoglycan of the tripeptide L-alanyl-gamma-D-glutamyl-2,6-meso-diaminoheptanedioate released during the maturation and constant remodeling of this bacterial cell wall polymer that occur during cell growth and division. The enzyme can also use the tetrapeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanine or the pentapeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanyl-D-alanine in vivo and in vitro. Requires Mg2+.
History
EC 6.3.2.45 created 2014
Orthology
K02558 UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase
Genes
ECOO : ECRM13514_5496(yjfG)
ECOH : ECRM13516_5115(yjfG)
EDJ : ECDH1ME8569_4089(mpl)
REE : electrica_04577(mpl)
RTG : NCTC13098_06660(mpl)
CLAP : NCTC11466_04050(mpl)
KIE : NCTC12125_03239(mpl)
BAGE : BADSM9389_36810(mpl)
AHN : NCTC12129_04390(mpl)
METY : MRY16398_50210(mpl)
PSHI : SAMEA2665130_2693(mpl)
YPI : YpsIP31758_3620(mpl)
SFJ : SAMEA4384070_0484(mpl)
SOF : NCTC11214_01138(mpl)
SERA : Ser39006_005595(mpl)
PARL : PEC302110_04960(mpl)
TPTY : NCTC11468_03244(mpl_1)
PHEI : NCTC12003_03646(mpl)
LRI : NCTC12151_03072(mpl)
HPIT : NCTC13334_01800(mpl)
HHZ : NCTC10839_01800(mpl)
HAEG : NCTC8502_01117(mpl)
ADP : NCTC12871_00081(mpl)
ALIG : NCTC10568_01737(mpl)
ASEG : NCTC10977_01913(mpl)
RPNE : NCTC8284_02954(mpl_1)
PAET : NCTC13378_01339(mpl)
XPH : XppCFBP6546_17015(mpl)
VAU : VANGNB10_cI2296c(mpl)
VPL : SA104470976_00284(mpl)
VIB : VspSTUT11_26510(mpl)
VIS : VspSTUT16_27690(mpl)
PPRC : PFLCHA0_c54370(mpl)
PMUD : NCTC8068_04659(mpl)
PFW : PF1751_v1c48550(mpl)
PTAE : NCTC10697_00605(mpl)
MCUN : NCTC10297_00556(mpl)
SCAA : TUM17387_30460(mpl)
LJR : NCTC11533_00924(mpl)
LCJ : NCTC11976_02039(mpl)
LWA : SAMEA4504053_1939(mpl)
LSS : NCTC12082_00984(mpl)
LADL : NCTC12735_00278(mpl)
LANT : TUM19329_21620(mpl)
MISZ : MishRS11D_18110(mpl)
HAAH : HALA3H3_830012(mpl)
AEL : NCTC12917_03520(mpl)
NME : NMB1145(mpl-1) NMB1183(mpl-2)
NMM : NMBM01240149_0983(mpl)
NMS : NMBM01240355_1110(mpl)
NMQ : NMBM04240196_1017(mpl)
NWE : SAMEA3174300_0309(mpl)
NZO : SAMEA4504057_1946(mpl)
NCI : NCTC10296_01966(mpl)
NCZ : NCTC10294_00748(mpl)
NANI : NCTC12227_00827(mpl)
ECOR : SAMEA4412678_1712(mpl)
BPM : BURPS1710b_3496(mpl)
BPL : BURPS1106A_3499(mpl)
PLG : NCTC10937_04801(mpl)
LMIR : NCTC12852_00936(mpl)
BUO : BRPE64_ACDS23630(mpl)
BTRM : SAMEA390648700730(mpl)
AXX : ERS451415_00631(mpl)
SMIO : CATMQ487_51940(mpl)
URU : DSM104443_04202(mpl)
UPL : DSM104440_03723(mpl)
THAG : CKCBHOJB_00753(mpl)
ABIW : Abiwalacus_07920(mpl)
ROO : G5S37_03195(mpl) G5S37_31455
LKB : LPTSP3_g30520(murC_2)
SDJ : NCTC13534_03250(mpl_1)
STHA : NCTC11429_00609(mpl)
PHG : KCTC32516_00257(mpl)
AALG : AREALGSMS7_00389(mpl)
KOS : KORDIASMS9_00101(mpl)
CGLE : NCTC11432_04474(mpl)
CTAI : NCTC12078_00095(mpl)
PTAN : CRYO30217_03036(mpl)
» show all
Taxonomy
Reference
Authors
Mengin-Lecreulx D, van Heijenoort J, Park JT
Title
Identification of the mpl gene encoding UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase in Escherichia coli and its role in recycling of cell wall peptidoglycan.
Journal
Sequence
Reference
Authors
Herve M, Boniface A, Gobec S, Blanot D, Mengin-Lecreulx D
Title
Biochemical characterization and physiological properties of Escherichia coli UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.45
ExPASy - ENZYME nomenclature database: 6.3.2.45