KEGG   ENZYME: 6.3.2.50Help
Entry
EC 6.3.2.50                 Enzyme                                 

Name
tenuazonic acid synthetase;
TAS1 (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
L-isoleucine:acetoacetyl-CoA ligase (tenuazonic acid-forming)
Reaction(IUBMB)
ATP + L-isoleucine + acetoacetyl-CoA = AMP + diphosphate + tenuazonic acid + CoA [RN:R11723]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
L-isoleucine [CPD:C00407];
acetoacetyl-CoA [CPD:C00332]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
tenuazonic acid [CPD:C08511];
CoA [CPD:C00010]
Comment
This fungal enzyme, isolated from Magnaporthe oryzae, is an non-ribosomal peptide synthetase (NRPS)-polyketide synthase (PKS) hybrid protein that consists of condensation (C), adenylation (A) and peptidyl-carrier protein (PCP) domains in the NRPS portion and a ketosynthase (KS) domain in the PKS portion. ATP is required for activation of isoleucine, which is then condensed with acetoacetyl-CoA. Cyclization and release from the enzyme are catalysed by the KS domain.
History
EC 6.3.2.50 created 2017
Orthology
K22581  tenuazonic acid synthetase
Genes
MGR: MGG_07803
Taxonomy
Reference
1  [PMID:26503170]
  Authors
Yun CS, Motoyama T, Osada H
  Title
Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS-PKS hybrid enzyme.
  Journal
Nat Commun 6:8758 (2015)
DOI:10.1038/ncomms9758
  Sequence
[mgr:MGG_07803]
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.50
IUBMB Enzyme Nomenclature: 6.3.2.50
ExPASy - ENZYME nomenclature database: 6.3.2.50
BRENDA, the Enzyme Database: 6.3.2.50

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