KEGG   ENZYME: 6.3.2.52Help
Entry
EC 6.3.2.52                 Enzyme                                 

Name
jasmonoyl---L-amino acid synthetase;
JAR1 (gene name);
JAR4 (gene name);
JAR6 (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
jasmonate:L-amino acid ligase
Reaction(IUBMB)
ATP + jasmonate + an L-amino acid = AMP + diphosphate + a jasmonoyl-L-amino acid [RN:R11950]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
jasmonate [CPD:C08491];
L-amino acid [CPD:C00151]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
jasmonoyl-L-amino acid [CPD:C21509]
Comment
Two jasmonoyl-L-amino acid synthetases have been described from Nicotiana attenuata [3] and one from Arabidopsis thaliana [1]. The N. attenuata enzymes generate jasmonoyl-L-isoleucine, jasmonoyl-L-leucine, and jasmonoyl-L-valine. The enzyme from A. thaliana could catalyse the addition of many different amino acids to jasmonate in vitro [1,4,5]. While the abundant form of jasmonate in plants is (-)-jasmonate, the active form of jasmonoyl-L-isoleucine is (+)-7-iso-jasmonoyl-L-isoleucine.
History
EC 6.3.2.52 created 2018
Orthology
K14506  jasmonic acid-amino synthetase
Genes
ATH: AT2G46370(JAR1)
ALY: ARALYDRAFT_483789
CRB: 17887579
CSAT: 104783209 104784678 104793611
EUS: EUTSA_v10001373mg
BRP: 103858212 103866275 103866475
BNA: 106392009 106415372 106438277 106438296 106438298 106441911 106446159 106450515 106453225
BOE: 106327971 106337574 106342461
RSZ: 108813808 108813817 108844535 108844539 108849225 108853684
THJ: 104802061 104817340
CPAP: 110810413
CIT: 102611440
TCC: 18614015
LJA: Lj1g3v5033430.1(Lj1g3v5033430.1) Lj1g3v5033430.2(Lj1g3v5033430.2) Lj1g3v5033430.3(Lj1g3v5033430.3) Lj3g3v2415310.1(Lj3g3v2415310.1) Lj3g3v2416320.1(Lj3g3v2416320.1)
FVE: 101296802
RCN: 112181918
PPER: 18784809
PMUM: 103332267
PAVI: 110751778
PXB: 103965897
CSV: 101212037
CMO: 103483208
RCU: 8263567
JCU: 105642878
QSU: 111985652
VVI: 100251627
INI: 109180806(JAR)
BVG: 104886569
OSA: 4339756
DOSA: Os05t0586200-01(Os05g0586200) Os11t0186500-00(Os11g0186500)
ATS: 109749018(LOC109749018) 109785424(LOC109785424)
ZMA: 100193303(cl2682_1) 100280367(pco121700) 100381512 100384080 103649563
ATR: 18426859
SMO: SELMODRAFT_124989(JAR1L8-1) SELMODRAFT_125145(JAR1L7-1) SELMODRAFT_128888(JAR1L7-2) SELMODRAFT_234778(JAR1L1-1) SELMODRAFT_31681(JAR1L3-1) SELMODRAFT_31683(JAR1L3-2) SELMODRAFT_427552(JAR1L8-2)
PPP: 112287761
 » show all
Taxonomy
Reference
1  [PMID:15258265]
  Authors
Staswick PE, Tiryaki I
  Title
The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis.
  Journal
Plant Cell 16:2117-27 (2004)
DOI:10.1105/tpc.104.023549
  Sequence
[ath:AT2G46370]
Reference
2  [PMID:17085687]
  Authors
Kang JH, Wang L, Giri A, Baldwin IT
  Title
Silencing threonine deaminase and JAR4 in Nicotiana attenuata impairs jasmonic acid-isoleucine-mediated defenses against Manduca sexta.
  Journal
Plant Cell 18:3303-20 (2006)
DOI:10.1105/tpc.106.041103
Reference
3  [PMID:17273867]
  Authors
Wang L, Halitschke R, Kang JH, Berg A, Harnisch F, Baldwin IT
  Title
Independently silencing two JAR family members impairs levels of trypsin proteinase inhibitors but not nicotine.
  Journal
Planta 226:159-67 (2007)
DOI:10.1007/s00425-007-0477-3
Reference
4  [PMID:17291501]
  Authors
Guranowski A, Miersch O, Staswick PE, Suza W, Wasternack C.
  Title
Substrate specificity and products of side-reactions catalyzed by jasmonate:amino acid synthetase (JAR1).
  Journal
FEBS Lett 581:815-20 (2007)
DOI:10.1016/j.febslet.2007.01.049
  Sequence
[ath:AT2G46370]
Reference
5  [PMID:18247047]
  Authors
Suza WP, Staswick PE
  Title
The role of JAR1 in Jasmonoyl-L: -isoleucine production during Arabidopsis wound  response.
  Journal
Planta 227:1221-32 (2008)
DOI:10.1007/s00425-008-0694-4
  Sequence
[ath:AT2G46370]
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.52
IUBMB Enzyme Nomenclature: 6.3.2.52
ExPASy - ENZYME nomenclature database: 6.3.2.52
BRENDA, the Enzyme Database: 6.3.2.52

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