KEGG   ENZYME: 6.3.2.52
Entry
EC 6.3.2.52                 Enzyme                                 
Name
jasmonoyl---L-amino acid ligase;
JAR1 (gene name);
JAR4 (gene name);
JAR6 (gene name);
jasmonoyl---L-amino acid synthetase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
Sysname
jasmonate:L-amino acid ligase
Reaction(IUBMB)
ATP + jasmonate + an L-amino acid = AMP + diphosphate + a jasmonoyl-L-amino acid [RN:R11950]
Reaction(KEGG)
R11950
Substrate
ATP [CPD:C00002];
jasmonate [CPD:C08491];
L-amino acid [CPD:C00151]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
jasmonoyl-L-amino acid [CPD:C21509]
Comment
Two jasmonoyl-L-amino acid synthetases have been described from Nicotiana attenuata [3] and one from Arabidopsis thaliana [1]. The N. attenuata enzymes generate jasmonoyl-L-isoleucine, jasmonoyl-L-leucine, and jasmonoyl-L-valine. The enzyme from A. thaliana could catalyse the addition of many different amino acids to jasmonate in vitro [1,4,5]. While the abundant form of jasmonate in plants is (-)-jasmonate, the active form of jasmonoyl-L-isoleucine is (+)-7-iso-jasmonoyl-L-isoleucine.
History
EC 6.3.2.52 created 2018, modified 2019
Orthology
K14506  jasmonic acid-amino synthetase
Genes
ATHAT2G46370(JAR1)
ALY9316303
CRB17887579
CSAT104783209 104784678 104793611
EUSEUTSA_v10001373mg
BRP103858212 103866275 103866475
BNA106392009 106415372 106438277 106438296 106438298 106441911 106446159 106450515 106453225
BOE106327971 106337574 106342461
RSZ108813808 108813817 108844535 108844539 108849225 108853684
THJ104802061 104817340
CPAP110810413
CIT102611440
CICCICLE_v10019459mg
PVY116121118
MINC123216584 123216586 123223803 123224207
TCC18614015
GRA105791543 105796195 105802782 105802783
GHI107900937 107909787 107909788 107923052 107923326 107933526 107952940 107954506
GAB108460785 108467411 108472614 108472695
DZI111304817 111313931
EGR104442158 104443381
GMX100777944 100794598 100806209 100813492
GSJ114390517 114400143 114407792 114418331
PVUPHAVU_006G110900g PHAVU_010G111400g
VRA106754140 106773156
VAR108318909 108346002
VUN114166370 114166795 114188102
CCAJ109797239 109808861
APRC113849948 113861096
MTRMTR_1g047730 MTR_7g117110 MTR_8g027920 MTR_8g027955 MTR_8g027965 MTR_8g027970 MTR_8g037720
CAM101500743 101504683 101506624 101506959
LJALj1g3v5033430.1(Lj1g3v5033430.1) Lj1g3v5033430.3(Lj1g3v5033430.3) Lj3g3v2415310.1(Lj3g3v2415310.1)
ADU107472946 107491964
AIP107623354 107645717
AHF112695332 112726459 112733726 112758416
LANG109333135 109357385 109357386 109359450 109359672
FVE101296802
RCN112181918
PPER18784809
PMUM103332267
PAVI110751778
PDUL117618563
MDM103413018 103439296
PXB103965897
ZJU107424151 107429109 107429170
MNT21386318
CSV101212037
CMO103483208
BHJ120085218
MCHA111016355 111025068
CMAX111473333 111475727 111479304 111479305
CMOS111432047 111432219 111446696
CPEP111790647 111790648 111800387
RCU8263567
JCU105642878
HBR110645079 110670437
MESC110614736 110615559
POP7494139 7497237
PEU105124788 105134892
PALZ118041545 118057695
JRE108985239 108996457
QSU111985652
QLO115986524
TWL119982545 119983271
VVI100251627
SLY101250455 101251682 101262053 101262357 101262663 101268624 101268670
SPEN107001869 107002523 107002524 107007864 107020374 107022768
SOT102578750 102600861 102603612
CANN107839284 107845604
NTA107761031 107769747 107772988 107774940 107798053 107798060 107806520 107811464
NSY104209986 104236492 104248247
NTO104084467 104090951 104096608 104110129 104110130
NAU109207440 109211394 109219431
INI109180806(JAR)
ITR116029416
SIND105173100 105173433
OEU111377782
EGT105956350 105963883
SSPL121761580 121775029 121777678 121804684 121806418
HAN110864821 110917855 110922359 110937608
ECAD122590706 122596687 122597751 122598238
LSV111876787 111894588
CCAV112522684 112526954 112529803
DCR108196757 108209455
CSIN114259899 114260773 114283086 114296732 114323877
BVG104886569
SOE110775233 110775235 110790433
CQI110714357 110714358 110714527 110725039 110725063 110725177
NNU104600319 104603960 109115461
MING122081186 122082359 122092725
TSS122639755 122645437 122664782
PSOM113323681 113340287
NCOL116260089
OSA4339756
DOSAOs05t0586200-01(Os05g0586200)
OBR102716971
BDI100821807
ATS109785424
TDC119294130 119349753
TAES123069945 123148866 123183299
TUA125531522
SBI8065295 8084842
ZMA100280367 100381512
SITA101771047 101779619 101781042
SVS117833908 117848434 117857665
PVIR120664477 120697698 120707450
PHAI112886030 112893299 112903383
PDA103708344 103708705 103716872
EGU105036519 105047558 105048226
MUS103980034 103980944 103981532 103992744 103996308
DCT110100668 110104139
PEQ110019693 110038389
AOF109828888 109847377 109847394
ATR18426859
SMOSELMODRAFT_124989(JAR1L8-1) SELMODRAFT_125145(JAR1L7-1) SELMODRAFT_125443(JAR1L1-2) SELMODRAFT_128888(JAR1L7-2) SELMODRAFT_21596(JAR1L6-2) SELMODRAFT_234778(JAR1L1-1) SELMODRAFT_235186(JAR1L2-2) SELMODRAFT_31681(JAR1L3-1) SELMODRAFT_31683(JAR1L3-2) SELMODRAFT_416246(JAR1L2-1) SELMODRAFT_427552(JAR1L8-2) SELMODRAFT_437924(JAR1L5-1) SELMODRAFT_441348(JAR1L5-2)
PPP112287761
 » show all
Reference
1  [PMID:15258265]
  Authors
Staswick PE, Tiryaki I
  Title
The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis.
  Journal
Plant Cell 16:2117-27 (2004)
DOI:10.1105/tpc.104.023549
  Sequence
[ath:AT2G46370]
Reference
2  [PMID:17085687]
  Authors
Kang JH, Wang L, Giri A, Baldwin IT
  Title
Silencing threonine deaminase and JAR4 in Nicotiana attenuata impairs jasmonic acid-isoleucine-mediated defenses against Manduca sexta.
  Journal
Plant Cell 18:3303-20 (2006)
DOI:10.1105/tpc.106.041103
Reference
3  [PMID:17273867]
  Authors
Wang L, Halitschke R, Kang JH, Berg A, Harnisch F, Baldwin IT
  Title
Independently silencing two JAR family members impairs levels of trypsin proteinase inhibitors but not nicotine.
  Journal
Planta 226:159-67 (2007)
DOI:10.1007/s00425-007-0477-3
Reference
4  [PMID:17291501]
  Authors
Guranowski A, Miersch O, Staswick PE, Suza W, Wasternack C.
  Title
Substrate specificity and products of side-reactions catalyzed by jasmonate:amino acid synthetase (JAR1).
  Journal
FEBS Lett 581:815-20 (2007)
DOI:10.1016/j.febslet.2007.01.049
  Sequence
[ath:AT2G46370]
Reference
5  [PMID:18247047]
  Authors
Suza WP, Staswick PE
  Title
The role of JAR1 in Jasmonoyl-L: -isoleucine production during Arabidopsis wound  response.
  Journal
Planta 227:1221-32 (2008)
DOI:10.1007/s00425-008-0694-4
  Sequence
[ath:AT2G46370]
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.52
IUBMB Enzyme Nomenclature: 6.3.2.52
ExPASy - ENZYME nomenclature database: 6.3.2.52
BRENDA, the Enzyme Database: 6.3.2.52

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