Entry
Name
biotin---[biotin carboxyl-carrier protein] ligase;
birA (gene name);
HLCS (gene name);
HCS1 (gene name);
biotin-[acetyl-CoA carboxylase] synthetase;
biotin-[acetyl coenzyme A carboxylase] synthetase;
acetyl coenzyme A holocarboxylase synthetase;
acetyl CoA holocarboxylase synthetase;
biotin:apocarboxylase ligase;
Biotin holoenzyme synthetase;
biotin:apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] ligase (AMP-forming);
biotin---[acetyl-CoA-carboxylase] ligase
Class
Ligases;
Forming carbon-nitrogen bonds;
Other carbon-nitrogen ligases
BRITE hierarchy
Sysname
biotin:apo-[carboxyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine [RN:
R04562 ]
Reaction(KEGG)
Substrate
ATP [CPD:
C00002 ];
biotin [CPD:
C00120 ];
[biotin carboxyl-carrier protein]-L-lysine [CPD:
C06249 ]
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
[biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine [CPD:
C06250 ]
Comment
The enzyme biotinylates a biotin carboxyl-carrier protein that is part of an acetyl-CoA carboxylase complex, enabling its subsequent carboxylation by EC
6.3.4.14 , biotin carboxylase. The carboxyl group is eventually transferred to acetyl-CoA by EC
2.1.3.15 , acetyl-CoA carboxytransferase. In some organisms the carrier protein is part of EC
6.4.1.2 , acetyl-CoA carboxylase.
History
EC 6.3.4.15 created 1978, modified 2018
Pathway
Orthology
K01942 biotin---protein ligase
K01947 biotin---[acetyl-CoA-carboxylase] ligase / type III pantothenate kinase
K03524 BirA family transcriptional regulator, biotin operon repressor / biotin---[acetyl-CoA-carboxylase] ligase
Genes
ORO : 101363115 101368585(HLCS)
BOM : 102275692 102276987(HLCS)
BIU : 109561544(HLCS) 109563420
PCAD : 102981930(HLCS) 114485348
ETL : 114070498(HLCS) 114071342 114072950
BGAR : 122930793 122932980
CGIB : 128013317 128020687 128020931
AOCE : 111587326 111587992
OKE : 118390677 118391077(hlcs)
PKI : 111855789 111858423(hlcs)
ARUT : 117406319 117407164
DSI : Dsimw501_GD19789(Dsim_GD19789)
CQU : CpipJ_CPIJ007071 CpipJ_CPIJ007072
ACEP : 105619994 105625408
BMAN : 114239389 114249541
NLU : 111048036 111050202 111058010
LPOL : 106461093 106469546
XEN : 124441389 124441538 124441541 124441869 124442559 124442932
ATH : AT1G37150(HCS2) AT2G25710(HCS1)
CSAT : 104703109 104714038 104752205 104752216
EUS : EUTSA_v10004827mg EUTSA_v10027838mg
BNA : 106368386 106392404 106409576 125585745
RSZ : 108805431 108830160 130501420
MINC : 123203292 123206093 123229157
HSYR : 120154372 120176365 120180396
MSYL : 126603611 126632037
PXB : 103933707 103956690 103956969
CPEP : 111780897 111808204
SDUL : 129903254 129903298
CANN : 107850582 107866583
INI : 109160079 109177408 109183075 109183459
SSPL : 121786473 121811335
SHIS : 125197468 125221333
APAN : 127246467 127249831
CSIN : 114266663 114315283
PSOM : 113346420 113356494
TAES : 123132409 123136485 123143975
MFLO : 136462754 136471168
SITA : 101752965 101769375
SMO : SELMODRAFT_80973 SELMODRAFT_88773
CRE : CHLRE_09g406000v5 CHLRE_12g508852v5
CVR : CHLNCDRAFT_135977 CHLNCDRAFT_26827
CGR : 2889255(GVI51_I03531)
NCS : NCAS_0A13910(NCAS0A13910)
NDI : NDAI_0A02120(NDAI0A02120)
TPF : TPHA_0A03060(TPHA0A03060)
TBL : TBLA_0F02180(TBLA0F02180)
TDL : TDEL_0C02310(TDEL0C02310)
KAF : KAFR_0B01050(KAFR0B01050)
KNG : KNAG_0C03960(KNAG0C03960)
LBG : 92207224(LODBEIA_P20280)
CAL : CAALFM_CR10620CA(CaO19.7645)
COT : CORT_0A05280 CORT_0D00250
YLI : 2912036(YALI2_E01146g)
NTE : NEUTE1DRAFT124604(NEUTE1DRAFT_124604)
SMP : 10807355(SMAC4_00725)
TATV : 25784141(TrAtP1_008469)
TASP : 36607621(TrAFT101_002933)
PLJ : 28884721(PLICBS_005936)
CDET : 87947867(CDEST_11367)
BFU : BCIN_01g05670(Bcbpl1)
PTRR : 6346849(PtrM4_146620)
CDEP : 91085118(L203_100904)
KMG : 30162798(I203_101347)
KNE : 92179657(IAR55_002398)
CCAC : CcaHIS019_0100680(BPL1)
ABP : AGABI1DRAFT114677(AGABI1DRAFT_114677)
ABV : AGABI2DRAFT226601(AGABI2DRAFT_226601)
SCM : SCHCO_02597546(SCHCODRAFT_02597546)
EHI : EHI_167730(26.t00001)
AAF : AURANDRAFT_64251(BPL2)
LMAT : 92511531(LSCM1_01403)
LOI : 92359141(LSCM4_03193)
LEIS : 94177038(GH5_01440)
LENR : 94169928(CUR178_02663)
LEIN : 94186393(JIQ42_02680)
PHET : 94289913(JKF63_03838)
ECOO : ECRM13514_5093(birA)
ECOH : ECRM13516_4829(birA)
ECW : EcE24377A_4512(birA)
EDJ : ECDH1ME8569_3837(birA)
ECOI : ECOPMV1_02816(birA)
REE : electrica_04837(birA)
RTG : NCTC13098_06960(birA)
CLAP : NCTC11466_04371(birA)
KIE : NCTC12125_03539(birA)
AHN : NCTC12129_04692(birA_1)
METY : MRY16398_53890(birA)
PTF : PROFFT_A_00700(birA)
PSHI : SAMEA2665130_2800(birA)
YPG : YpAngola_A3396(birA)
YPI : YpsIP31758_3871(birA)
SMW : SMWW4_v1c02700(birA)
SFJ : SAMEA4384070_0247(birA)
SOF : NCTC11214_02069(birA)
SGRI : SGBXF1_00286(birA_1)
SSAR : SSARUM_000228(birA)
RACE : JHW33_15085(birA) JHW33_19555
RBAD : H2866_09370(birA) H2866_22065
PAQU : DMB82_0000925(birA)
PARL : PEC302110_37730(birA)
EHD : ERCIPSTX3056_318(birA)
TPTY : NCTC11468_00174(birA_2)
PHEI : NCTC12003_03811(birA)
PRJ : NCTC6933_03832(birA)
OPO : DSM2777_00460 DSM2777_01815
LRI : NCTC12151_03328(birA)
HIC : NTHIC486_00772(birA)
HPIT : NCTC13334_01485(birA)
HHZ : NCTC10839_00097(birA)
HAEG : NCTC8502_01011(birA)
PDAG : 4362423_00277(birA)
ADP : NCTC12871_00117(birA)
ALIG : NCTC10568_02246(birA)
ASEG : NCTC10977_01635(birA)
AVT : NCTC3438_01163(birA)
RPNE : NCTC8284_01495(birA_1)
PAET : NCTC13378_00886(birA)
XFH : XFHB_06345 XFHB_07030
XFU : XFF4834R_chr38840(birA)
XPH : XppCFBP6546_14415(birA)
XHD : LMG31886_41310(birA)
LLZ : LYB30171_02364(birA)
VAU : VANGNB10_cI0201(birA)
VPL : SA104470976_02408(birA)
VIB : VspSTUT11_01100(birA)
VIS : VspSTUT16_01280(birA)
PAEB : NCGM1900_0674(birA)
PPRC : PFLCHA0_c55540(birA)
PMUD : NCTC8068_04810(birA)
PFW : PF1751_v1c49670(birA)
PPUU : PputUW4_04900(birA)
PTRT : HU722_0026705(birA)
PTAE : NCTC10697_00499(birA)
MBS : MRBBS_0595(birA) MRBBS_3429(birA)
MSX : AU14_11125 AU14_19000
MARI : ACP86_11190 ACP86_12970
MLQ : ASQ50_09105 ASQ50_14525
MSQ : BKP64_12555 BKP64_13420
MARA : D0851_14500 D0851_15535
MARJ : MARI_23370(birA) MARI_24720
MALL : PBN92_01795 PBN92_02995
MSHE : MAALD49_04210 MAALD49_06800(birA)
MSAN : LPB19_05255 LPB19_06185
PSYC : DABAL43B_0406(birA)
MCAT : MC25239_00294(birA)
MCUN : NCTC10297_00831(birA)
SCAA : TUM17387_01800(birA)
SALH : HMF8227_00417(birA)
CBS : COXBURSA331_A0937(birA)
ALG : AQULUS_16790(birA_1) AQULUS_18790(birA_2)
LLG : 44548918_01735(birA)
LJR : NCTC11533_01909(birA)
LCJ : NCTC11976_02805(birA)
LWA : SAMEA4504053_1111(birA)
LSS : NCTC12082_00787(birA)
LADL : NCTC12735_01362(birA)
LANT : TUM19329_13490(birA)
MISZ : MishRS11D_22360(birA)
FTW : FTW_0828(birA) FTW_1593
FTH : FTH_1249(birA1) FTH_1532(birA2)
FTI : FTS_1249(birA) FTS_1545(birA2)
FTO : X557_06625 X557_08155
FTN : FTN_0568 FTN_0811(birA)
FCF : FNFX1_0558 FNFX1_0820
FCN : FN3523_0510 FN3523_0974
FHI : FSC454_02530 FSC454_05190
FRT : F7308_1313 F7308_1772
FNL : M973_03365 M973_05225
FHA : CDV26_04210 CDV26_07700
FRX : F7310_02950 F7310_04995
FRM : BBG19_0836 BBG19_1228
FAD : CDH04_07090 CDH04_09705
FMI : F0R74_01225 F0R74_03565
FOO : CGC45_04240 CGC45_06165
FNN : FSC774_01615 FSC774_02580
FSR : KQR59_02775 KQR59_05135
FRL : FIP56_03175 FIP56_05165
FGU : SD28_04645 SD28_07070
AFRI : E3E15_04475 E3E15_05265
AII : E4K63_02545 E4K63_04770
THIO : AYJ59_02430 AYJ59_03400
TFRI : Thiofri_03229(birA)
TNI : TVNIR_2068(birA_[H])
SPIZ : GJ672_00220 GJ672_08455
SHAI : LMH63_00295 LMH63_15805
GAI : IMCC3135_32050(birA)
THIC : TspCOW1_30660(birA)
HAXI : HAALTHF_06530n(birA)
ALCA : ASALC70_01516(birA)
LLP : GH975_01300 GH975_09970
AEL : NCTC12917_00232(birA)
CHJ : NCTC10426_01693(birA)
CDIZ : CEDIAZO_03481(birA)
NMX : NMA510612_0379(birA)
NWE : SAMEA3174300_2026(birA)
NZO : SAMEA4504057_0113(birA)
NCI : NCTC10296_02408(birA)
NCZ : NCTC10294_01398(birA)
NANI : NCTC12227_00087(birA)
KKI : KKKWG1_0538(birA_coaX)
ECOR : SAMEA4412678_1183(birA)
PLG : NCTC10937_04890(birA)
LMIR : NCTC12852_00638(birA)
BTRM : SAMEA390648701370(birA)
AXX : ERS451415_06018(birA)
COF : FOZ74_07405 FOZ74_12105
LIM : L103DPR2_00261(birA)
LIH : L63ED372_00205(birA)
SIMP : C6571_08500 C6571_12410
SMIO : CATMQ487_05310(birA)
SUTK : FG381_07910 FG381_12170
SWS : I6J16_01175 I6J16_10585
URU : DSM104443_00632(birA)
UPL : DSM104440_00611(birA)
THAG : CKCBHOJB_00550(birA)
APHA : WSQ_03955 WSQ_04035
ERW : ERWE_CDS_02560(birA)
ERG : ERGA_CDS_02520(birA)
REN : EF513_05520 EF513_05970(birA)
NAF : GQ61_01570 GQ61_06370
KMN : HW532_20000 HW532_20195
RBS : RHODOSMS8_00864(birA)
SMEL : SM2011_c01928(birA)
SFD : USDA257_c33130(birA)
SAME : SAMCFNEI73_Ch1395(birA)
AGR : AGROH133_05700(birA)
AVV : RvVAT039_28930(birA)
AVF : RvVAR031_16320(birA)
REC : RHECIAT_CH0001691(birA)
REL : REMIM1_CH01639(birA)
REP : IE4803_CH01619(birA)
REI : IE4771_CH01672(birA)
RGA : RGR602_CH01480(birA)
RPHA : AMC79_CH01650(birA)
NEN : NCHU2750_12870(birA)
HOE : IMCC20628_01665 IMCC20628_03304
BIC : LMTR13_07930 LMTR13_18805
RHOL : RBJ75_05380 RBJ75_08065
VGO : GJW-30_1_02776(birA)
BHS : BM1374165_00965(birA)
BTX : BM1374166_01137(birA)
BEZ : NCTC12898_00755(birA)
MAQU : Maq22A_c02905(birA)
FIL : BN1229_v1_1612 BN1229_v1_3002
FIY : BN1229_v1_1615 BN1229_v1_2914
PLEO : OHA_1_00315(birA_1)
HDI : HDIA_1381(birA_1) HDIA_1752(birA_2)
BRN : D1F64_08805 D1F64_10440
LABR : CHH27_06870 CHH27_14710
LABP : FJ695_08580 FJ695_16275
LAGG : B0E33_17460 B0E33_25560
SIW : GH266_00210 GH266_01900
BVY : NCTC9239_01899(birA)
TSV : DSM104635_02069(birA)
RLI : RLO149_c024860(birA)
PHP : PhaeoP97_01027(birA)
PPIC : PhaeoP14_00973(birA)
YPAC : CEW88_09045 CEW88_21045
YAN : AYJ57_02625 AYJ57_22875
TPRO : Ga0080559_TMP2136 Ga0080559_TMP4767
SULD : B5M07_10905 B5M07_17395
SPOT : G6548_00685 G6548_11095
SINL : DSM14862_01186(birA_1)
SPSE : SULPSESMR1_01848(birA)
TOM : BWR18_08680 BWR18_20670
RMM : ROSMUCSMR3_00512(birA)
AHT : ANTHELSMS3_03105(birA) ANTHELSMS3_03750
MALU : KU6B_19480 KU6B_23880(birA)
LALG : LentiSH36_00995(birA)
RCP : RCAP_rcc01538(birA1) RCAP_rcp00131(birA2)
LVS : LOKVESSMR4R_01784(birA)
SALO : EF888_00920 EF888_13410
LIT : FPZ52_03365 FPZ52_09290
PPRU : FDP22_12020 FDP22_15075
PAMO : BAR1_05225 BAR1_15570
POZ : I0K15_02710 I0K15_15540
HDH : G5B40_01605 G5B40_09180
SBIN : SBA_ch1_22890(birA)
SPAG : sphantq_03454(birA)
SFLA : SPHFLASMR4Y_01106(birA)
AZZ : DEW08_06050 DEW08_20150
SKT : IGS68_03190 IGS68_13350
HCL : NCTC13205_01355(birA)
HSH : NHP194022_09090(birA)
HPUL : NCTC13154_00983(birA)
CJV : MTVDSCj20_0096(birA)
CFV : CFVI03293_1567(birA)
CAMP : CFT03427_1493(birA)
CCOC : CCON33237_0447(birA)
CLR : UPTC16701_0193(birA)
CLM : UPTC16712_0191(birA)
CAMR : CAQ16704_0190(birA)
CHV : CHELV3228_0060(birA)
CPIN : CPIN18020_1218(birA)
CAMZ : CVIC12175_0339(birA)
GBN : GEOBRER4_21730(birA)
PSYF : N1030_04580 N1030_14120
DSB : LN040_02430 LN040_10310
DVU : DVU_1835 DVU_2557(birA)
DVG : Deval_1470 Deval_2358
CLIH : KPS_000537 KPS_001200
DLM : DPPLL_01130 DPPLL_24120
DAK : DaAHT2_0465 DaAHT2_2450
DBK : DGMP_00440 DGMP_25220
ECOM : QTN59_08775 QTN59_09640
EAJ : Q3M24_14330 Q3M24_22950
DTO : TOL2_C02960(birA) TOL2_C12270(birA2)
DEK : DSLASN_30120 DSLASN_37050(birA)
TID : Thein_0948 Thein_1909