| Entry |
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| Name |
tRNAIle-lysidine synthase;
TilS;
mesJ (gene name);
yacA (gene name);
isoleucine-specific transfer ribonucleate lysidine synthetase;
tRNAIle-lysidine synthetase
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| Class |
Ligases;
Forming carbon-nitrogen bonds;
Other carbon-nitrogen ligases
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| Sysname |
L-lysine:[tRNAIle2]-cytidine34 ligase (AMP-forming)
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| Reaction(IUBMB) |
[tRNAIle2]-cytidine34 + L-lysine + ATP = [tRNAIle2]-lysidine34 + AMP + diphosphate + H2O [RN: R09597]
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| Reaction(KEGG) |
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| Substrate |
[tRNAIle2]-cytidine34;
L-lysine [CPD: C00047];
ATP [CPD: C00002]
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| Product |
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| Comment |
The bacterial enzyme modifies the wobble base of the CAU anticodon of tRNAIle at the oxo group in position 2 of cytidine34. This modification determines both codon and amino acid specificities of tRNAIle.
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| History |
EC 6.3.4.19 created 2011
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| Orthology |
| K04075 | tRNA(Ile)-lysidine synthase |
| K15780 | bifunctional protein TilS/HprT |
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| Genes |
» show all
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| Reference |
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| Authors |
Ikeuchi Y, Soma A, Ote T, Kato J, Sekine Y, Suzuki T |
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| Title |
molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition. |
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| Journal |
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| Reference |
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| Authors |
Salowe SP, Wiltsie J, Hawkins JC, Sonatore LM |
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| Title |
The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Nakanishi K, Fukai S, Ikeuchi Y, Soma A, Sekine Y, Suzuki T, Nureki O |
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| Title |
Structural basis for lysidine formation by ATP pyrophosphatase accompanied by a lysine-specific loop and a tRNA-recognition domain. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Soma A, Ikeuchi Y, Kanemasa S, Kobayashi K, Ogasawara N, Ote T, Kato J, Watanabe K, Sekine Y, Suzuki T |
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| Title |
An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Nakanishi K, Bonnefond L, Kimura S, Suzuki T, Ishitani R, Nureki O |
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| Title |
Structural basis for translational fidelity ensured by transfer RNA lysidine synthetase. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 6.3.4.19 |
| ExPASy - ENZYME nomenclature database: | 6.3.4.19 |
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