KEGG   ENZYME: 6.3.4.22
Entry
EC 6.3.4.22                 Enzyme                                 
Name
tRNAIle2-agmatinylcytidine synthase;
TiaS;
AF2259;
tRNAIle-2-agmatinylcytidine synthetase;
tRNAIle-agm2C synthetase;
tRNAIle-agmatidine synthetase
Class
Ligases;
Forming carbon-nitrogen bonds;
Other carbon-nitrogen ligases
Sysname
agmatine:[tRNAIle]-cytidine34 ligase
Reaction(IUBMB)
ATP + agmatine + [tRNAIle2]-cytidine34 + H2O = [tRNAIle2]-2-agmatinylcytidine34 + AMP + 2 phosphate [RN:R10496]
Reaction(KEGG)
R10496
Substrate
ATP [CPD:C00002];
agmatine [CPD:C00179];
[tRNAIle2]-cytidine34;
H2O [CPD:C00001]
Product
[tRNAIle2]-2-agmatinylcytidine34;
AMP [CPD:C00020];
phosphate [CPD:C00009]
Comment
The enzyme from the archaeon Archaeoglobus fulgidus modifies the wobble base of the CAU anticodon of the archaeal tRNAIle2 at the oxo group in position 2 of cytidine34. This modification is crucial for accurate decoding of the genetic code. In bacteria EC 6.3.4.19, tRNAIle-lysidine synthase, catalyses the modification of [tRNAIle2]-cytidine34 to [tRNAIle2]-lysidine34 .
History
EC 6.3.4.22 created 2013
Orthology
K06932  tRNA(Ile2)-agmatinylcytidine synthase
Genes
MJAMJ_1095
MFEMefer_1336
MVUMetvu_1118
MFSMFS40622_0162
MIFMetin_0603
MJHJH146_0033
MESGMLAUSG7_0809(tiaS)
MESAMLASG1_0384(tiaS)
MIGMetig_0478
MMPMMP0616
MMQMmarC5_0989
MMXMmarC6_0275
MMZMmarC7_1639
MMDGYY_03390
MMAKMMKA1_06660
MMAOMMOS7_06610
MMADMMJJ_03670
MAEMaeo_0755
MVNMevan_1489
MVOMvol_0248
MOKMetok_0217
METFCFE53_03130
MTHMTH_966
MMGMTBMA_c13520
METCMTCT_0877
MWOMWSIV6_1356(tiaS)
METEtca_00929
METZMETMT2_0998
METKFVF72_06765
MTHMFZP57_06470
METJFZP68_04195
MSTMsp_1142
METBAW729_10065
MRUmru_0685
MSIMsm_0452
MEBAbm4_0413
MMILsm9_0558
MEYETL18_02620
MOLYLM1_0329
MAROMarbSA_17680
MELMetbo_0426
MEWMSWAN_1961
METHMBMB1_1591(tiaS)
MFCBRM9_0826
MFIDSM1535_0789(tiaS)
MCUBMCBB_1889(tiaS)
MSUBBK009_06950
METNBK008_02590
METTCIT01_02575
METOCIT02_09995
MEMEHYG87_03930
MFVMfer_0668
MKAMK1003
AFUAF_2259
AFGAFULGI_00025540
APOArcpr_0648
AVEArcve_1578
ASTAsulf_01007
FPLFerp_1288
GACGACE_1471
GAHGAH_01052
PFUPF1855
PFIPFC_09315
PHOPH1812(PH1812)
PABPAB0247
PYNPNA2_0411
PYAPYCH_01040
PYSPy04_1738
PYCTQ32_07680
TKOTK0553
TONTON_0699
TGATGAM_1029
TSITSIB_1197
TBATERMP_00575
THEGQS_01425
THATAM4_479
THMCL1_2002
TLTOCC_01114
THSTES1_0950
TNUBD01_0657
TEUTEU_08925
TGYX802_09150
THVADU37_CDS09310
TCHCHITON_2099
TPEPA0127_01940
TPIEA7C91_09615
TGGA3K92_03195
TCEA3L02_03845
TBSA3L01_04265
THHCDI07_04160
TSLA3L11_08280
TTDA3L14_06160
TPRFA3L09_07025
TRLA3L10_02330
TPAFA3L08_07615
THYA3L12_07690
TICFH039_07920
TCQTIRI35C_1105(tiaS)
THEMFPV09_01495
PPACPAP_07755
MBAMbar_A2319
MBYMSBRM_1009
MBWMSBRW_2425
MBARMSBR2_1401
MBAKMSBR3_1473
MACMA_3525
MMAMM_0445
MMJMSMAS_0858
MMACMSMAC_0847
MVCMSVAZ_1055
MEKMSKOL_1005
MLSMSLAZ_0746
METMMSMTP_2364
MEFMSWH1_0641
MEQMSWHS_0661
MSJMSSAC_3216
MSZMSSIH_1046
MSWMSSIT_1093
MTHRMSTHT_1079
MTHEMSTHC_2224
MHORMSHOH_3068
MFZAOB57_011030
MBUMbur_1810
MMETMCMEM_0061
MELOJ7W08_10385
MMHMmah_1918
MHAZBHR79_04050
MEVMetev_0063
MZHMzhil_0058
MPYMpsy_1823
MZIHWN40_12975
MHZMetho_2324
MTPMthe_0611
MCJMCON_1904
MHIMhar_0187
MHUMhun_2547
MRTJKHC33_03945
MLAMlab_0159
MEMMemar_2348
MBGBN140_2487
MEMAMMAB1_3347(tiaS)
MCHKMchiMG62_00780
MPIMpet_0081
MBNMboo_0194
MFOMetfor_2916
MPLMpal_0030
MPDMCP_2871
MEZMtc_0281
RCIRCIX1429
HALVNG_2110C
HSLOE_3943R(tiaS)
HDLHALDL1_15450
HHBHhub_3400(tiaS)
HAGSJT689_09830
HABOJRZ79_08210
HNOLT974_13820
HLULT972_00925
HANRLJ422_08480
HJEHacjB3_01490
HALHHTSR_1874
HHSRHSR6_1944(tiaS)
HSUHLASF_0230
HSFHLASA_0230
SALRFQU85_05615
HALREFA46_002530
HARAAArcS_2537(tiaS)
HAADMW046_08200
HALXM0R89_06930
HMArrnAC2518
HHIHAH_2948
HHNHISP_14990
HABSG26_07105
HTABVU17_14330
HALJG9465_03480
HSINKDQ40_14520
NPHNP_1318A(tiaS)
NMONmlp_3253(tiaS)
NHOHWV23_06135
NSALHWV07_07860
HUTHuta_1790
HTIHTIA_1320
HALAHrd1104_11295
HMUHmuk_0845
HALZE5139_07405
HALLLC1Hm_0272(tiaS)
HAZPGBQ70_07400
HALIBV210_08930
HSNDV733_09715
HDSHSR122_2452(tiaS)
HRRHZS55_03390
HPELHZS54_04090
HLTI7X12_10240
HARCHARCEL1_13145
HDOMUK72_04960
HWAHQ_3056A(tiaS)
HWCHqrw_3574(tiaS)
HVOHVO_0339(tiaS)
HMEHFX_0324
HGIABY42_01535
HALEG3A49_01910
HBOHbor_28320
HAQDU484_04065
HAJDU500_04545
HAERDU502_02260
HRAEI982_11255
HLMDV707_02645
HALMFCF25_08435
HLAHlac_0272
HALPDOS48_01445
HALBEKH57_10265
HEZZEO776_01165
HALQHrr1229_005530
HSSJ7656_00015
HSAIHPS36_05490
HAZZKI388_14840
SRUBC2R22_01410
HAEhalTADL_2102
HALNB4589_010000
HALGHUG10_01770
HALUHUG12_15320
HMPK6T50_15165
HREK6T36_15165
HRMK6T25_01180
HACBHbl1158_07255
HDFAArcSl_0862(tiaS)
HAHHalar_3068
HTUHtur_2426
HDABB347_08245
HJTDVR14_16035 DVR14_20155
HALYHYG82_05250
HSALJMJ58_16720
HLOJ0X27_17760
HAKZJ0X25_00650
NMGNmag_1178(tiaS)
HXAHalxa_4156
NATNJ7G_1333
NPENatpe_1372
NVRFEJ81_07255
NPLFGF80_04085
NAYHYG81_03335
NGENatgr_2460
HRUHalru_2032
NOUNatoc_1343
SALIL593_15020
SAIMK0C01_04760
HLRHALLA_08665
HLCCHINAEXTREME10490
NAJB1756_16205
NAGAArcMg_2515
NANAArc1_1212
NBGDV706_11465
NASGCU68_00660
MELUMTLP_08490
MEAMMU439_00920
TACTa0545
TVOTVG0590669(TVG0590669)
PTOPTO1377
FACFACI_IFERC01G1593
FAIFAD_0572(tiaS)
CDIVCPM_0360
TARTALC_01575
MAXMMALV_16950
MERMMINT_19450
MEARMpt1_c14580(tiaS2)
MARCAR505_0003
ABIAboo_0928
ACFAciM339_0076
APEAPE_0656.1
ACJACAM_0488
SMRSmar_1362
SHCShell_1082
IHOIgni_0309
IISEYM_07450
DKADKAM_0469
DFDDesfe_0589
DMUDesmu_0728
TAGTagg_0667
IAGIgag_0055
THGTCELL_0822
HBUHbut_0008
PFMPyrfu_0159
PDLPyrde_0549
STOSTK_03120
SOHD1869_01120
SSOSSO0258
SOLSsol_1234
SSOASULA_1275
SSOLSULB_1276
SSOFSULC_1274
SSHIJ5U23_03210
SCASSACC_01760
SAISaci_0732
SACNSacN8_03555
SACRSacRon12I_03540
SACSSUSAZ_03310
SISLS215_1991
SIAM1425_1882
SIMM1627_1959
SIDM164_1889
SIYYG5714_1958
SINYN1551_0962
SIILD85_2103
SIHSiH_1819
SIRSiRe_1739
SICSiL_1732
SULABFU36_04715
SULEGFS03_10310
SULOGFS33_02125
SULLEWF20_02585
MSEMsed_2296
MCNMcup_2039
MHKDFR87_06960
MPRUDFR88_06700
MTENGWK48_05200
MJNMjAS7_0586
AHOAhos_0781
AMANB6F84_07360
ABRIDFR85_07565
ASULDFR86_06420
AAMBD1866_11735
ACIHHS5_21550
SACDHS1genome_0236
SAZOD1868_01910
CSTYKN1_18760
STEPIC006_1793
SAHSHS7_07740
PAIPAE0933
PISPisl_1170
PCLPcal_0100
PASPars_0133
PYRP186_2378
POGPogu_2352
TNETneu_0126
PYWPYWP30_00136
CMACmaq_0160
TTNTTX_0548
TUZTUZN_0192
VDIVdis_1829
VMOVMUT_0270
TPETpen_0604
THBN186_04390
TCBTCARB_1905
THELIG193_04485
ASCASAC_1057
ACIASE86_06420
CLGCalag_0571
FFOFFONT_0807
NMRNmar_1562
NIRNSED_00695
NKRNKOR_08500
NIDNPIRD3C_0493(tiaS)
NIWNisw_06090
NCLC5F47_08190
NOXC5F49_08075
NUEC5F50_11435
NIPNsoK4_08340
NCTNMSP_0197(tiaS)
NICDSQ20_09100
CSYCENSYa_2023
NGANgar_c35910
NVNNVIE_020600
NEVNTE_00071
TAANMY3_00488
NFNNFRAN_1460(tiaS)
NCVNCAV_0167(tiaS)
CSUCSUB_C1139
NBVT478_1284
TAHSU86_004625
NIUDSQ19_01395
NDVNDEV_1925(tiaS)
CCAINAS2_0020
BARCAOA65_1257(tiaS_1) AOA65_1587(tiaS_2)
BARBAOA66_1422(tiaS)
 » show all
Reference
1  [PMID:20139989]
  Authors
Ikeuchi Y, Kimura S, Numata T, Nakamura D, Yokogawa T, Ogata T, Wada T, Suzuki T, Suzuki T
  Title
Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea.
  Journal
Nat Chem Biol 6:277-82 (2010)
DOI:10.1038/nchembio.323
  Sequence
[afu:AF_2259] [pho:PH1812]
Reference
2  [PMID:22002222]
  Authors
Terasaka N, Kimura S, Osawa T, Numata T, Suzuki T
  Title
Biogenesis of 2-agmatinylcytidine catalyzed by the dual protein and RNA kinase TiaS.
  Journal
Nat Struct Mol Biol 18:1268-74 (2011)
DOI:10.1038/nsmb.2121
Reference
3  [PMID:22102245]
  Authors
Osawa T, Inanaga H, Kimura S, Terasaka N, Suzuki T, Numata T
  Title
Crystallization and preliminary X-ray diffraction analysis of an archaeal tRNA-modification enzyme, TiaS, complexed with tRNA(Ile2) and ATP.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 67:1414-6 (2011)
DOI:10.1107/S1744309111034890
Other DBs
ExplorEnz - The Enzyme Database: 6.3.4.22
IUBMB Enzyme Nomenclature: 6.3.4.22
ExPASy - ENZYME nomenclature database: 6.3.4.22
BRENDA, the Enzyme Database: 6.3.4.22

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