KEGG   ENZYME: 6.3.5.12Help
Entry
EC 6.3.5.12                 Enzyme                                 

Name
Ni-sirohydrochlorin a,c-diamide synthase;
cfbB (gene name)
Class
Ligases;
Forming carbon-nitrogen bonds;
Carbon-nitrogen ligases with glutamine as amido-N-donor
BRITE hierarchy
Sysname
Ni-sirohydrochlorin:L-glutamine amido-ligase (ADP-forming)
Reaction(IUBMB)
2 ATP + Ni-sirohydrochlorin + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + Ni-sirohydrochlorin a,c-diamide + 2 L-glutamate [RN:R11627]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
Ni-sirohydrochlorin [CPD:C21510];
L-glutamine [CPD:C00064];
H2O [CPD:C00001]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
Ni-sirohydrochlorin a,c-diamide [CPD:C21511];
L-glutamate [CPD:C00025]
Comment
The enzyme, studied from the methanogenic archaeon Methanosarcina acetivorans, participates in the biosynthesis of the nickel-containing tetrapyrrole cofactor coenzyme F430, which is required by EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase.
History
EC 6.3.5.12 created 2017
Pathway
ec00860  Porphyrin and chlorophyll metabolism
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K22012  Ni-sirohydrochlorin a,c-diamide synthase
Genes
MAC: MA_3626(cbiA2)
MBA: Mbar_A0348
MBY: MSBRM_0571
MBW: MSBRW_0649
MBAR: MSBR2_1808
MBAK: MSBR3_2863
MMA: MM_0513
MMJ: MSMAS_0793
MMAC: MSMAC_0772
MVC: MSVAZ_3045
METM: MSMTP_0885
MTHE: MSTHC_0830
MTHR: MSTHT_2452
MHOR: MSHOH_0896
MBU: Mbur_1039(cbiA_cobB)
MMET: MCMEM_1521
MMH: Mmah_1414
MPY: Mpsy_0957
MTP: Mthe_1179
MCJ: MCON_0502(cobB1)
MHI: Mhar_0046
MHU: Mhun_0671
MLA: Mlab_0521
MBG: BN140_0717(cobB)
MPI: Mpet_1003
MBN: Mboo_1420
MPL: Mpal_0678
MPD: MCP_0365(cobB)
MEZ: Mtc_0065(cbiA-1)
RCI: RCIX2001(cbiA-1)
NCT: NMSP_0082
 » show all
Taxonomy
Reference
1  [PMID:27846569]
  Authors
Zheng K, Ngo PD, Owens VL, Yang XP, Mansoorabadi SO.
  Title
The biosynthetic pathway of coenzyme F430 in methanogenic and methanotrophic archaea.
  Journal
Science 354:339-342 (2016)
DOI:10.1126/science.aag2947
  Sequence
[mac:MA_3626]
Other DBs
ExplorEnz - The Enzyme Database: 6.3.5.12
IUBMB Enzyme Nomenclature: 6.3.5.12
ExPASy - ENZYME nomenclature database: 6.3.5.12
BRENDA, the Enzyme Database: 6.3.5.12

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