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Entry
EC 6.3.5.13                 Enzyme                                 

Name
lipid II isoglutaminyl synthase (glutamine-hydrolysing);
MurT/GatD;
MurT/GatD complex
Class
Ligases;
Forming carbon-nitrogen bonds;
Carbon-nitrogen ligases with glutamine as amido-N-donor
BRITE hierarchy
Sysname
beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol:L-glutamine amidoligase (ADP-forming)
Reaction(IUBMB)
ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O = ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate (overall reaction) [RN:R05030];
(1a) L-glutamine + H2O = L-glutamate + NH3 [RN:R00256];
(1b) ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol = ADP + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol [RN:R12330];
(1c) beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + NH3 = beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + phosphate [RN:R12331]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol [CPD:C05893];
L-glutamine [CPD:C00064];
H2O [CPD:C00001];
beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol [CPD:C22083];
NH3 [CPD:C00014]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol;
L-glutamate [CPD:C00025];
NH3 [CPD:C00014];
beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol [CPD:C05894]
Comment
The enzyme complex, found in Gram-positive bacteria, consists of two subunits. A glutaminase subunit (cf. EC 3.5.1.2, glutaminase) produces an ammonia molecule that is channeled to a ligase subunit, which adds it to the activated D-glutamate residue of lipid II, converting it to an isoglutamine residue.
History
EC 6.3.5.13 created 2019
Pathway
ec00550  Peptidoglycan biosynthesis
ec01100  Metabolic pathways
Orthology
K07009  lipid II isoglutaminyl synthase (glutamine-hydrolysing)
K23393  lipid II isoglutaminyl synthase (glutamine-hydrolysing)
Genes
CHJ: NCTC10426_00626
DAV: DESACE_05250 DESACE_05255
SDO: SD1155_11470 SD1155_11475
BWW: bwei_5444 bwei_5445
SAU: SA1707 SA1708
SAV: SAV1891 SAV1892
SAW: SAHV_1876 SAHV_1877
SAH: SaurJH1_1979 SaurJH1_1980
SAJ: SaurJH9_1945 SaurJH9_1946
SAM: MW1832 MW1833
SHH: ShL2_00947(murE2) ShL2_00948
SHU: SHYC_04365(murE-1) SHYC_04370
LLA: L113931(cobQ) L114717(ylbD)
LLK: LLKF_1132(cobQ) LLKF_1133(ylbD)
LLT: CVCAS_1078(cobQ) CVCAS_1079(ylbD)
LLS: lilo_1008(cobQ) lilo_1009(ylbD)
LLM: llmg_1465(murC2) llmg_1466(cobQ)
LLI: uc509_1106(cobQ) uc509_1107(murC2)
LLW: kw2_1051(cobQ) kw2_1052
LLJ: LG36_1272(ylbD) LG36_1273(cobQ)
LPK: LACPI_1219(murT) LACPI_1220(gatD)
SPYA: A20_0800c(cobQ) A20_0801c(murE)
SPG: SpyM3_0667(cobQ) SpyM3_0668(murC)
SOZ: Spy49_0813c(cobQ) Spy49_0814c(murC2)
SPR: spr1443 spr1444(cobQ)
SGC: A964_0888 A964_0889(murE)
SMU: SMU_1429(murC2) SMU_1430(cobQ)
SMC: SmuNN2025_0671(cobQ) SmuNN2025_0672(murC2)
SMJ: SMULJ23_0679(cobQ) SMULJ23_0680(murC2)
STC: str1254 str1255(cobQ)
STL: stu1254 stu1255(cobQ)
SSA: SSA_0800(cobQ) SSA_0801
SEZ: Sez_1216
SDC: SDSE_1217 SDSE_1218(cobQ)
SGT: SGGB_1453(cobQ)
STB: SGPB_1356(murE.1) SGPB_1357(cobQ)
SCF: Spaf_0759 Spaf_0760(murE)
SIE: SCIM_1000 SCIM_1001(cobQ)
LPJ: JDM1_1990 JDM1_1991(cobQ)
LAC: LBA0765 LBA1882(cobQ)
LAF: SD55_0785 SD55_1874(cobQ)
LSL: LSL_0587 LSL_0588(murE)
LSI: HN6_00524 HN6_00525(murE)
LSJ: LSJ_0634c LSJ_0635c(murE)
LDB: Ldb0699(murE2) Ldb2100
LCB: LCABL_01970(cobQ) LCABL_13750(murC)
LHL: LBHH_1946
LRH: LGG_00240(cobQ) LGG_01168(murC)
LRL: LC705_00231(cobQ) LC705_01188(murC)
LKE: WANG_0911(murE1) WANG_1404
LRM: LRC_13700 LRC_13710(cobQ)
EFS: EFS1_2062 EFS1_2063(cobQ)
ENE: ENT_17630
EMU: EMQU_2363 EMQU_2364(cobQ)
LCI: LCK_01469(murC2) LCK_01470
LGS: LEGAS_0390 LEGAS_0391(murE2)
CRN: CAR_c17090(murD2) CAR_c17100
CML: BN424_778 BN424_779(murE)
JDA: BW727_101109(murF_1) BW727_101110(cobQ)
CTYK: CTK_C06530(cobQ) CTK_C06540
HHW: NCTC503_02120(murE1) NCTC503_02121(cobQ_2)
HSC: HVS_10510(murC2) HVS_10515
BPRO: PMF13cell1_03110(cobQ_1) PMF13cell1_03111(murD_1)
PTH: PTH_1649 PTH_1650(MurE)
TTE: TTE0007 TTE0008(MurE)
TPZ: Tph_c22240 Tph_c22250(murE2)
MTU: Rv3712 Rv3713(cobQ2)
MTC: MT3815 MT3816(cobQ-2)
MRA: MRA_3749 MRA_3750(cobQ2)
MTUR: CFBS_3934 CFBS_3935(cobQ2)
MTD: UDA_3712 UDA_3713(cobQ2)
MTUH: I917_26035
MTUT: HKBT1_3918 HKBT1_3919(cobQ2)
MTUU: HKBT2_3928 HKBT2_3929(cobQ2)
MTQ: HKBS1_3931 HKBS1_3932(cobQ2)
MBB: BCG_3772 BCG_3773(cobQ2)
MBT: JTY_3774 JTY_3775(cobQ2)
MAF: MAF_37210 MAF_37220(cobQ2)
MCE: MCAN_37341 MCAN_37351(cobQ2)
MUL: MUL_4304 MUL_4305(cobQ2)
MMI: MMAR_5229 MMAR_5230(cobQ2)
MLI: MULP_05510 MULP_05511(cobQ2)
MSHG: MSG_04722 MSG_04723(cobQ2)
MSG: MSMEI_6112 MSMEI_6113(cobQ2)
MPHL: MPHLCCUG_00325(cobQ_1) MPHLCCUG_00326(murE_1)
MHAS: MHAS_03930(murD_2) MHAS_03931(cbiA)
MSAL: DSM43276_00273(cobB_1) DSM43276_00274(murD_1)
CGL: NCgl0242(Cgl0245) NCgl0243(Cgl0246)
CGB: cg0299(cobQ) cg0300
CGU: WA5_0242(CobQ) WA5_0243
CGM: cgp_0299(cobQ) cgp_0300
CPL: Cp3995_0156(cobQ) Cp3995_0157(murD)
CPG: Cp316_0168(cobQ) Cp316_0169(murD)
CPP: CpP54B96_0159(cobQ) CpP54B96_0160(murD)
CPK: Cp1002_0154(cobQ) Cp1002_0155(murD)
CPQ: CpC231_0157(cobQ) CpC231_0158(murD)
CPX: CpI19_0156(cobQ1) CpI19_0157(murD1)
CPZ: CpPAT10_0156(cobQ) CpPAT10_0157(murD)
COR: Cp267_0163(cobQ) Cp267_0164(murD)
COP: Cp31_0170(cobQ1) Cp31_0171(murD1)
COD: Cp106_0155(cobQ) Cp106_0156(murD)
COS: Cp4202_0152(cobQ) Cp4202_0153(murD)
COI: CpCIP5297_0165(cobQ1) CpCIP5297_0166(murD1)
COE: Cp258_0166(cobQ) Cp258_0167(murD)
COU: Cp162_0160(cobQ1) Cp162_0161(murD1)
CUN: Cul210932_0218(cobQ1) Cul210932_0219(murD1)
CUS: CulFRC11_0205(cobQ1) CulFRC11_0206(murD1)
CUQ: Cul210931_0210(cobQ1) Cul210931_0211(murD1)
CUZ: Cul05146_0225(cobQ1) Cul05146_0226(murD1)
CUJ: CUL131002_0204c(cobQ1) CUL131002_0205c(murD1)
CAQU: CAQU_00695
RFA: A3L23_02355(cobQ_1) A3L23_02356(murD_1)
RHS: A3Q41_01013(murD_1) A3Q41_01014(cobQ_1)
RHU: A3Q40_03526(murD_2) A3Q40_03527(cobQ_2)
GOM: D7316_03959(cobQ_2) D7316_03960(murD_2)
SCO: SCO1212(2SCG58.12) SCO1213(2SCG58.13)
SRW: TUE45_01603 TUE45_01604(cobB_1)
SLE: sle_58840(sle_58840) sle_58850(sle_58850)
SRN: A4G23_00509 A4G23_00510(cobB_1)
SLX: SLAV_31340(cobQ2) SLAV_31345
SGE: DWG14_07281(cobB_4) DWG14_07282(murD_2)
CMC: CMN_01627(murX)
PFR: PFREUD_07830(cobB_cobQ) PFREUD_07840
ACIJ: JS278_01132(cobQ_1) JS278_01133(murF_1)
FAL: FRAAL0637(murD) FRAAL0638(cobQ)
SEN: SACE_3574 SACE_3575(cobQ-2)
BLJ: BLD_1230 BLD_1231(murE2)
BDE: BDP_0193(murE) BDP_0194
BBI: BBIF_1628 BBIF_1629(murE)
BBP: BBPR_1687 BBPR_1688(murE)
BBF: BBB_1685(cobQ) BBB_1686(murD)
BBRU: Bbr_0246(murE) Bbr_0247
BBRS: BS27_0254(murE) BS27_0255
GPA: GPA_27550
AEQ: AEQU_2041
PCAT: Pcatena_03410(murE_1) Pcatena_03420(cobQ)
CBAC: JI75_00265
SYY: SYNGTS_1206(slr1742) SYNGTS_1823(slr0938)
SYT: SYNGTI_1206(slr1742) SYNGTI_1823(slr0938)
SYS: SYNPCCN_1205(slr1742) SYNPCCN_1822(slr0938)
SYQ: SYNPCCP_1205(slr1742) SYNPCCP_1822(slr0938)
HHG: XM38_045820(murC) XM38_045830(cobQ_2)
DOU: BMF77_03621(murC_1) BMF77_04207
MEB: Abm4_0872
MMIL: sm9_0107
MCUB: MCBB_1398(cobQ3) MCBB_1399
NDV: NDEV_1437
 » show all
Taxonomy
Reference
1  [PMID:22291598]
  Authors
Munch D, Roemer T, Lee SH, Engeser M, Sahl HG, Schneider T
  Title
Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus.
  Journal
PLoS Pathog 8:e1002509 (2012)
DOI:10.1371/journal.ppat.1002509
  Sequence
Reference
2  [PMID:30154570]
  Authors
Noldeke ER, Muckenfuss LM, Niemann V, Muller A, Stork E, Zocher G, Schneider T, Stehle T
  Title
Structural basis of cell wall peptidoglycan amidation by the GatD/MurT complex of Staphylococcus aureus.
  Journal
Sci Rep 8:12953 (2018)
Reference
3  [PMID:30093673]
  Authors
Morlot C, Straume D, Peters K, Hegnar OA, Simon N, Villard AM, Contreras-Martel C, Leisico F, Breukink E, Gravier-Pelletier C, Le Corre L, Vollmer W, Pietrancosta N, Havarstein LS, Zapun A
  Title
Structure of the essential peptidoglycan amidotransferase MurT/GatD complex from  Streptococcus pneumoniae.
  Journal
Nat Commun 9:3180 (2018)
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.3.5.13
IUBMB Enzyme Nomenclature: 6.3.5.13
ExPASy - ENZYME nomenclature database: 6.3.5.13
BRENDA, the Enzyme Database: 6.3.5.13

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