KEGG ENZYME: 6.4.1.10

ENZYME: 6.4.1.10

Entry

EC 6.4.1.10                 Enzyme

Name

atromentin synthase;
atromentin synthetase;
invA1 (gene name);
invA2 (gene name);
invA5 (gene name);
greA (gene name);
atrA (gene name)

Class

Ligases;
Forming carbon-carbon bonds;
Ligases that form carbon-carbon bonds (only sub-subclass identified to date)

Sysname

3-(4-hydroxyphenyl)pyruvate:3-(4-hydroxyphenyl)pyruvate ligase (atromentin-forming)

Reaction(IUBMB)

2 ATP + 2 3-(4-hydroxyphenyl)pyruvate = 2 AMP + 2 diphosphate + atromentin (overall reaction) [RN:R13505];
(1a) ATP + 3-(4-hydroxyphenyl)pyruvate + [atromentin synthase] = AMP + diphosphate + 3-(4-hydroxyphenyl)pyruvoyl-[atromentin synthase] [RN:R13506];
(1b) ATP + 3-(4-hydroxyphenyl)pyruvate + 3-(4-hydroxyphenyl)pyruvoyl-[atromentin synthase] = AMP + diphosphate + atromentin + [atromentin synthase] [RN:R13507]

Reaction(KEGG)

R13505 R13506 R13507

Substrate

ATP [CPD:C00002];
3-(4-hydroxyphenyl)pyruvate [CPD:C01179];
[atromentin synthase];
3-(4-hydroxyphenyl)pyruvoyl-[atromentin synthase]

Product

AMP [CPD:C00020];
diphosphate [CPD:C00013];
atromentin [CPD:C23042];
3-(4-hydroxyphenyl)pyruvoyl-[atromentin synthase];
[atromentin synthase]

Comment

Requires Mg2+.The enzyme, found in some fungi such as members of the Boletales and Eurotiales order, is a non-ribosomal peptide synthase-like enzyme comprising three domains - an adelylation (A) domain, an ayl-carrier protein (ACP) domain, and a thioesterase (TE) domain. The enzyme activates 3-(4-hydroxyphenyl)pyruvate by adenylation and catalyses the dimerization/cyclization of two such molecules to form atromentin. Atromentin is a precursor for assorted metabolites produced by the fungi, including pulvinic acids and 2,5-diarylcyclopentenones.

History

EC 6.4.1.10 created 2025

Pathway

ec00350

Tyrosine metabolism

Orthology

K28347

atromentin synthase

Genes

CPUT:

CONPUDRAFT_170203 CONPUDRAFT_67809