KEGG   ENZYME: 6.4.1.6
Entry
EC 6.4.1.6                  Enzyme                                 

Name
acetone carboxylase
Class
Ligases;
Forming carbon-carbon bonds;
Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
Sysname
acetone:carbon-dioxide ligase (AMP-forming)
Reaction(IUBMB)
acetone + hydrogen carbonate + 2 ATP + 3 H2O = acetoacetate + 2 AMP + 4 phosphate [RN:R05735]
Reaction(KEGG)
R05735
Substrate
acetone [CPD:C00207];
hydrogen carbonate [CPD:C00288];
ATP [CPD:C00002];
H2O [CPD:C00001]
Product
acetoacetate [CPD:C00164];
AMP [CPD:C00020];
phosphate [CPD:C00009]
Comment
Requires Mg2+ and ATP. The reaction involves separate phosphorylation of hydrogencarbonate and acetone, forming carboxyphosphate and phosphoenolacetone, respectively, which are combined to form the final product. The enzyme from Xanthobacter sp. strain Py2 also carboxylates butan-2-one to 3-oxopentanoate.
History
EC 6.4.1.6 created 2001
Orthology
K10854  acetone carboxylase, alpha subunit
K10855  acetone carboxylase, beta subunit
K10856  acetone carboxylase, gamma subunit
Genes
GHO: AL542_03630 AL542_03635 AL542_03640
AVN: Avin_20910(acxA) Avin_20920(acxB) Avin_20930(acxC)
AVL: AvCA_20910(acxA) AvCA_20920(acxB) AvCA_20930(acxC)
AVD: AvCA6_20910(acxA) AvCA6_20920(acxB) AvCA6_20930(acxC)
ACX: Achr_24430(acxC) Achr_24440(acxB) Achr_24450(acxA)
PAR: Psyc_1448 Psyc_1450(acxC)
PPIS: B1L02_05720 B1L02_05725 B1L02_05730
GAG: Glaag_3576
KIM: G3T16_12200
AEH: Mlg_1029 Mlg_1030 Mlg_1031
CNC: CNE_2c13060(acxA) CNE_2c13070(acxB) CNE_2c13080(acxC) CNE_BB1p08470(acxC)
RME: Rmet_4105(acxA) Rmet_4106(acxB) Rmet_4107(acxC)
BOK: DM82_4513(acxC) DM82_4514(acxB) DM82_4515(acxA)
BMK: DM80_3943
BCT: GEM_3539
AMIM: MIM_c06390(acxA) MIM_c06400(acxB) MIM_c06410(acxC)
EBA: ebA4700(acxA) ebA4701(acxB) ebA4702(acxC)
ABRE: pbN1_07560(acxC) pbN1_07570(acxB) pbN1_07580(acxA)
APET: ToN1_19230(acxC) ToN1_19240(acxB) ToN1_19250(acxA)
AZA: AZKH_0561(acxA) AZKH_0562(acxB) AZKH_0563(acxC) AZKH_3165(apc) AZKH_3167(apc)
HPY: HP0697
HPJ: jhp_0631
HPL: HPB8_896(hyuA3) HPB8_897(acxB) HPB8_898(acxC)
HAC: Hac_0949 Hac_0950(hyuB)
PLA: Plav_1150
SME: SM_b20407(hyuB) SM_b20408
HMC: HYPMC_0473(acxC) HYPMC_0474(acxB) HYPMC_0475(acxA)
MMED: Mame_04504(acxA) Mame_04505(acxB) Mame_04506(acxC)
RCP: RCAP_rcc01336(acxA) RCAP_rcc01337(acxB) RCAP_rcc01338(acxC)
SMIC: SmB9_09020
BMET: BMMGA3_02520(acxA) BMMGA3_02525(acxB) BMMGA3_02530(acxC)
BFD: NCTC4823_02247(acxC) NCTC4823_02248(acxB_4) NCTC4823_02249(acxA_4)
MVA: Mvan_4899
PDX: Psed_5912
PAUT: Pdca_35830
RXY: Rxyl_1849
 » show all
Reference
1  [PMID:9237998]
  Authors
Sluis MK, Ensign SA.
  Title
Purification and characterization of acetone carboxylase from Xanthobacter strain Py2.
  Journal
Proc Natl Acad Sci U S A 94:8456-61 (1997)
DOI:10.1073/pnas.94.16.8456
  Sequence
Reference
2  [PMID:22020645]
  Authors
Schuhle K, Heider J.
  Title
Acetone and butanone metabolism of the denitrifying bacterium "Aromatoleum aromaticum" demonstrates novel biochemical properties of an ATP-dependent aliphatic ketone carboxylase.
  Journal
J Bacteriol 194:131-41 (2012)
DOI:10.1128/JB.05895-11
Other DBs
ExplorEnz - The Enzyme Database: 6.4.1.6
IUBMB Enzyme Nomenclature: 6.4.1.6
ExPASy - ENZYME nomenclature database: 6.4.1.6
BRENDA, the Enzyme Database: 6.4.1.6
CAS: 189258-15-9

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