KEGG   ENZYME: 6.4.1.7Help
Entry
EC 6.4.1.7                  Enzyme                                 

Name
2-oxoglutarate carboxylase;
oxalosuccinate synthetase;
carboxylating factor for ICDH (incorrect);
CFI;
OGC
Class
Ligases;
Forming carbon-carbon bonds;
Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
BRITE hierarchy
Reaction(IUBMB)
ATP + 2-oxoglutarate + HCO3- = ADP + phosphate + oxalosuccinate [RN:R08201]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
2-oxoglutarate [CPD:C00026];
HCO3- [CPD:C00288]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
oxalosuccinate [CPD:C05379]
Comment
A biotin-containing enzyme that requires Mg2+ for activity. It was originally thought [1] that this enzyme was a promoting factor for the carboxylation of 2-oxoglutarate by EC 1.1.1.41, isocitrate dehydrogenase (NAD+), but this has since been disproved [2]. The product of the reaction is unstable and is quickly converted into isocitrate by the action of EC 1.1.1.41 [2].
History
EC 6.4.1.7 created 2006
Orthology
K20140  2-oxoglutarate carboxylase large subunit
K20141  2-oxoglutarate carboxylase small subunit
Genes
AAE: aq_1517(pycB) aq_1520(pycA)
HYA: HY04AAS1_1434 HY04AAS1_1435
HHO: HydHO_1415 HydHO_1416
HYS: HydSN_1453 HydSN_1454
HTH: HTH_1392(cfiA) HTH_1393(cfiB)
HTE: Hydth_1382 Hydth_1383
TAL: Thal_0267 Thal_0268
TRD: THERU_03935 THERU_03940
SAF: SULAZ_0523 SULAZ_0524(accC_2)
Taxonomy
Reference
1  [PMID:14731279]
  Authors
Aoshima M, Ishii M, Igarashi Y.
  Title
A novel biotin protein required for reductive carboxylation of 2-oxoglutarate by isocitrate dehydrogenase in Hydrogenobacter thermophilus TK-6.
  Journal
Mol Microbiol 51:791-8 (2004)
DOI:10.1046/j.1365-2958.2003.03863.x
  Sequence
Reference
2  [PMID:17076668]
  Authors
Aoshima M, Igarashi Y.
  Title
A novel oxalosuccinate-forming enzyme involved in the reductive carboxylation of 2-oxoglutarate in Hydrogenobacter thermophilus TK-6.
  Journal
Mol Microbiol 62:748-59 (2006)
DOI:10.1111/j.1365-2958.2006.05399.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.4.1.7
IUBMB Enzyme Nomenclature: 6.4.1.7
ExPASy - ENZYME nomenclature database: 6.4.1.7
BRENDA, the Enzyme Database: 6.4.1.7
CAS: 60382-75-4

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