KEGG   ENZYME: 7.2.4.5Help
Entry
EC 7.2.4.5                  Enzyme                                 

Name
glutaconyl-CoA decarboxylase;
glutaconyl coenzyme A decarboxylase;
pent-2-enoyl-CoA carboxy-lyase;
4-carboxybut-2-enoyl-CoA carboxy-lyase
Class
Translocases;
Catalysing the translocation of inorganic cations;
Linked to decarboxylation
BRITE hierarchy
Sysname
(2E)-4-carboxybut-2-enoyl-CoA carboxy-lyase [(2E)-but-2-enoyl-CoA-forming]
Reaction(IUBMB)
(2E)-4-carboxybut-2-enoyl-CoA + Na+[side 1] = (2E)-but-2-enoyl-CoA + CO2 + Na+[side 2] [RN:R03028]
Reaction(KEGG)
Substrate
(2E)-4-carboxybut-2-enoyl-CoA [CPD:C02411];
Na+[side 1]
Product
(2E)-but-2-enoyl-CoA [CPD:C00877];
CO2 [CPD:C00011];
Na+[side 2]
Comment
The enzyme from the bacterium Acidaminococcus fermentans is a biotinyl-protein, requires Na+, and acts as a sodium pump. Prior to the Na+-dependent decarboxylation, the carboxylate is transferred to biotin in a Na+-independent manner. The conserved lysine, to which biotin forms an amide bond, is located 34 amino acids before the C-terminus, flanked on both sides by two methionine residues, which are conserved in every biotin-dependent enzyme.
History
EC 7.2.4.5 created 1986 as EC 4.1.1.70, modified 2003, transferred 2019 to EC 7.2.4.5
Pathway
ec00362  Benzoate degradation
ec00650  Butanoate metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K01615  glutaconyl-CoA decarboxylase subunit alpha
Genes
GBM: Gbem_1453(gcdA)
GEO: Geob_0238(gcdA)
GEM: GM21_2800
DML: Dmul_25060(gcdA)
DAT: HRM2_38510(gcdA1) HRM2_38640(gcdA2)
DTO: TOL2_C38230
SAT: SYN_00481
DTI: Desti_2254 Desti_2615
DBR: Deba_2446
CAPR: EQM14_12255
CSO: CLS_16270
FAA: HMPREF0389_01471(gcdA)
OVA: OBV_10900
BPRS: CK3_23950
PED: ING2D1G_1422(gcdA)
PHAR: NCTC13077_00176(gcdA)
AIN: Acin_0318
FNU: FN0204
 » show all
Taxonomy
Reference
1  [PMID:6628393]
  Authors
Buckel W, Semmler R.
  Title
Purification, characterisation and reconstitution of glutaconyl-CoA decarboxylase, a biotin-dependent sodium pump from anaerobic bacteria.
  Journal
Eur J Biochem 136:427-34 (1983)
DOI:10.1111/j.1432-1033.1983.tb07760.x
Reference
2  [PMID:11248185]
  Authors
Buckel W.
  Title
Sodium ion-translocating decarboxylases.
  Journal
Biochim Biophys Acta 1505:15-27 (2001)
DOI:10.1016/S0005-2728(00)00273-5
Other DBs
ExplorEnz - The Enzyme Database: 7.2.4.5
IUBMB Enzyme Nomenclature: 7.2.4.5
ExPASy - ENZYME nomenclature database: 7.2.4.5
BRENDA, the Enzyme Database: 7.2.4.5
CAS: 84399-93-9

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