KEGG   ENZYME: 7.2.4.5
Entry
EC 7.2.4.5                  Enzyme                                 
Name
glutaconyl-CoA decarboxylase;
glutaconyl coenzyme A decarboxylase;
pent-2-enoyl-CoA carboxy-lyase;
4-carboxybut-2-enoyl-CoA carboxy-lyase
Class
Translocases;
Catalysing the translocation of inorganic cations;
Linked to decarboxylation
Sysname
(2E)-4-carboxybut-2-enoyl-CoA carboxy-lyase [(2E)-but-2-enoyl-CoA-forming]
Reaction(IUBMB)
(2E)-4-carboxybut-2-enoyl-CoA + Na+[side 1] = (2E)-but-2-enoyl-CoA + CO2 + Na+[side 2] [RN:R03028]
Reaction(KEGG)
R03028
Substrate
(2E)-4-carboxybut-2-enoyl-CoA [CPD:C02411];
Na+[side 1]
Product
(2E)-but-2-enoyl-CoA [CPD:C00877];
CO2 [CPD:C00011];
Na+[side 2]
Comment
The enzyme from the bacterium Acidaminococcus fermentans is a biotinyl-protein, requires Na+, and acts as a sodium pump. Prior to the Na+-dependent decarboxylation, the carboxylate is transferred to biotin in a Na+-independent manner. The conserved lysine, to which biotin forms an amide bond, is located 34 amino acids before the C-terminus, flanked on both sides by two methionine residues, which are conserved in every biotin-dependent enzyme.
History
EC 7.2.4.5 created 1986 as EC 4.1.1.70, modified 2003, transferred 2019 to EC 7.2.4.5
Pathway
ec00362  Benzoate degradation
ec00650  Butanoate metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K01615  glutaconyl-CoA decarboxylase subunit alpha
Genes
GEMGM21_2800
GEOGeob_0238(gcdA)
GBMGbem_1453(gcdA)
GBNGEOBRER4_29690
GSUBKP001_01980
DMLDmul_25060(gcdA)
DATHRM2_38510(gcdA1) HRM2_38640(gcdA2)
DTOTOL2_C38230
DOVDSCO28_25370 DSCO28_61390
DWDDSCW_22800
DALKDSCA_21110 DSCA_57350
DLIdnl_11250(gcdA)
DMMdnm_079260(gcdA)
SATSYN_00481
DTIDesti_2254 Desti_2615
DBRDeba_2446
CAPREQM14_12255
OVAOBV_10900
CSOCLS_16270
FAAHMPREF0389_01471(gcdA)
DORDesor_1189 Desor_1195 Desor_1197
FWADCMF_06350
LASAL9O85_09830
BPRSCK3_23950
PEDING2D1G_1422(gcdA)
PHARNCTC13077_00176(gcdA)
AFNAcfer_1817
AINAcin_0318
TRMJO41_09895
TPHGFUT81_01915
TRZGWP43_09965
TMEDDWB79_07260
FNUFN0204
FNCHMPREF0946_01480
FNTHMPREF0405_01480
FUSHMPREF0409_01246
FNEFSDG_02107
FHWRN87_11195
FPDCTM68_10285
FGOC4N16_07335
FNFBSQ88_06950
FPEIC4N17_03505
FCII6I83_03780
 » show all
Reference
1  [PMID:6628393]
  Authors
Buckel W, Semmler R.
  Title
Purification, characterisation and reconstitution of glutaconyl-CoA decarboxylase, a biotin-dependent sodium pump from anaerobic bacteria.
  Journal
Eur J Biochem 136:427-34 (1983)
DOI:10.1111/j.1432-1033.1983.tb07760.x
Reference
2  [PMID:11248185]
  Authors
Buckel W.
  Title
Sodium ion-translocating decarboxylases.
  Journal
Biochim Biophys Acta 1505:15-27 (2001)
DOI:10.1016/S0005-2728(00)00273-5
Other DBs
ExplorEnz - The Enzyme Database: 7.2.4.5
IUBMB Enzyme Nomenclature: 7.2.4.5
ExPASy - ENZYME nomenclature database: 7.2.4.5
BRENDA, the Enzyme Database: 7.2.4.5
CAS: 84399-93-9

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