KEGG   ENZYME: 7.5.2.5Help
Entry
EC 7.5.2.5                  Enzyme                                 

Name
ABC-type lipopolysaccharide transporter;
lptB (gene name);
lipopolysaccharide transport system;
lipopolysaccharide-transporting ATPase
Class
Translocases;
Catalysing the translocation of carbohydrates and their derivatives;
Linked to the hydrolysis of a nucleoside triphosphate
BRITE hierarchy
Sysname
ATP phosphohydrolase (ABC-type, lipopolysaccharide-exporting)
Reaction(IUBMB)
ATP + H2O + lipopolysaccharide[side 1] = ADP + phosphate + lipopolysaccharide[side 2]
Substrate
ATP [CPD:C00002];
H2O [CPD:C00001];
lipopolysaccharide[side 1]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
lipopolysaccharide[side 2]
Comment
An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. Does not undergo phosphorylation during the transport process. The enzyme, characterized from the bacterium Escherichia coli, functions as part of the lipopolysaccharide (LPS) export system, a seven protein system that translocates LPS from the inner- to the outer membrane. The ATPase activity in this system is implicated in releasing LPS from the inner membrane.
History
EC 7.5.2.5 created 2000 as EC 3.6.3.39, transferred 2018 to EC 7.5.2.5
Reference
1  [PMID:17056748]
  Authors
Sperandeo P, Cescutti R, Villa R, Di Benedetto C, Candia D, Deho G, Polissi A
  Title
Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli.
  Journal
J Bacteriol 189:244-53 (2007)
DOI:10.1128/JB.01126-06
  Sequence
[eco:b3200 b3201]
Reference
2  [PMID:18375759]
  Authors
Ruiz N, Gronenberg LS, Kahne D, Silhavy TJ
  Title
Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli.
  Journal
Proc Natl Acad Sci U S A 105:5537-42 (2008)
DOI:10.1073/pnas.0801196105
  Sequence
[eco:b4261 b4262]
Reference
3  [PMID:19500581]
  Authors
Narita S, Tokuda H
  Title
Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides.
  Journal
FEBS Lett 583:2160-4 (2009)
DOI:10.1016/j.febslet.2009.05.051
  Sequence
Reference
4  [PMID:20720015]
  Authors
Tran AX, Dong C, Whitfield C
  Title
Structure and functional analysis of LptC, a conserved membrane protein involved  in the lipopolysaccharide export pathway in Escherichia coli.
  Journal
J Biol Chem 285:33529-39 (2010)
DOI:10.1074/jbc.M110.144709
  Sequence
[eco:b3199]
Reference
5  [PMID:23138981]
  Authors
Okuda S, Freinkman E, Kahne D
  Title
Cytoplasmic ATP hydrolysis powers transport of lipopolysaccharide across the periplasm in E. coli.
  Journal
Science 338:1214-7 (2012)
DOI:10.1126/science.1228984
Reference
6  [PMID:20446753]
  Authors
Chng SS, Gronenberg LS, Kahne D
  Title
Proteins required for lipopolysaccharide assembly in Escherichia coli form a transenvelope complex.
  Journal
Biochemistry 49:4565-7 (2010)
DOI:10.1021/bi100493e
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 7.5.2.5
IUBMB Enzyme Nomenclature: 7.5.2.5
ExPASy - ENZYME nomenclature database: 7.5.2.5
BRENDA, the Enzyme Database: 7.5.2.5

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