KEGG   ENZYME: 1.1.1.145Help
Entry
EC 1.1.1.145                Enzyme                                 

Name
3beta-hydroxy-Delta5-steroid dehydrogenase;
progesterone reductase;
Delta5-3beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene steroid dehydrogenase;
3beta-hydroxy steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid oxidoreductase;
3beta-hydroxy-5-ene-steroid oxidoreductase;
steroid-Delta5-3beta-ol dehydrogenase;
3beta-HSDH;
5-ene-3-beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene-steroid dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3beta-hydroxy-Delta5-steroid:NAD+ 3-oxidoreductase
Reaction(IUBMB)
a 3beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH + H+ [RN:R03176]
Reaction(KEGG)
Substrate
3beta-hydroxy-Delta5-steroid [CPD:C03836];
NAD+ [CPD:C00003]
Product
3-oxo-Delta5-steroid [CPD:C01034];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
This activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Delta5-3-hydroxy steroids to a Delta4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form (cf. EC 5.3.3.1, steroid Delta-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
History
EC 1.1.1.145 created 1972
Pathway
ec00140  Steroid hormone biosynthesis
ec00984  Steroid degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00070  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
K16045  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
Genes
HSA: 3283(HSD3B1) 3284(HSD3B2)
PTR: 457165 469446
PPS: 100982464(HSD3B2) 100983009 100990187
GGO: 101141801 101146563(HSD3B2)
PON: 100451655 100452394
NLE: 100596087 100605995(HSD3B2) 100606563(HSD3B1) 100607955
MCC: 712686(HSD3B2) 713091(HSD3B1)
MCF: 102129497 102133592(HSD3B1) 102134747 102135110
CSAB: 103224100(HSD3B1) 103224104 103224107 103224109(HSD3B2)
RRO: 104654599 104654605
MMU: 100043456(Gm10681) 15492(Hsd3b1) 15493(Hsd3b2) 15495(Hsd3b4) 15496(Hsd3b5) 15497(Hsd3b6)
RNO: 108348086 24470(Hsd3b5) 29632(Hsd3b2) 360348(Hsd3b1) 682974(Hsd3b3)
HGL: 101707488(Hsd3b1) 101707858
CFA: 483146(HSD3B2)
AML: 100464280(HSD3B1)
UMR: 103677123
ORO: 101378904
FCA: 100616070(HSD3B2)
PTG: 102957262
AJU: 106978496
BTA: 281824(HSD3B1)
BOM: 102274088
BIU: 109555859
PHD: 102328070
CHX: 100860869(3BHSD)
OAS: 100190978(HSD3B1)
SSC: 445539(HSD3B1)
CFR: 102512447
CDK: 105089475
BACU: 103015346
LVE: 103083046
OOR: 101284283
ECB: 100034078(HSD3B2)
EPZ: 103548379
EAI: 106832920(HSD3B2)
MYB: 106723550(HSD3B2)
HAI: 109389868(HSD3B2)
RSS: 109446820(HAO2)
PALE: 102891879
LAV: 100659049(HSD3B2)
TMU: 101358325
MDO: 100012668
SHR: 100913530(HSD3B2)
OAA: 100080135
GGA: 396015(HSD3B1)
MGP: 104915630
CJO: 107319373(3beta-HSD)
ACYG: 106045741
TGU: 751770(HSD3B1)
GFR: 102039409
FAB: 101817697
PHI: 102101526
PMAJ: 107211459
CCAE: 111938581
CCW: 104687106
FPG: 101921564
FCH: 102052697
CLV: 102087460
EGZ: 104126518
AAM: 106497227
ASN: 102367786(HSD3B1)
AMJ: 109280168(HSD3B1)
PSS: 102453952
CMY: 102934871
CPIC: 101946936
ACS: 100561899(hsd3b1)
PVT: 110076926
PBI: 103067418
GJA: 107114856
XLA: 734818(hsd3b1.L)
XTR: 100490622
NPR: 108802532
DRE: 373131(hsd3b2) 553532(hsd3b1)
SRX: 107712267
SANH: 107658243
SGH: 107569293
CCAR: 109052439
IPU: 100304475(HSD3B)
AMEX: 103027852(hsd3b1)
TRU: 101069884(hsd3b1)
LCO: 104921488(hsd3b1)
MZE: 101485719
OLA: 100144358(3bhsd)
XMA: 102228303
PRET: 103477979
NFU: 107389612(hsd3b1)
KMR: 108244192(hsd3b1)
CSEM: 103391994(hsd3b1)
LCF: 108902437
SDU: 111218347
HCQ: 109522071
BPEC: 110156192(hsd3b1)
MALB: 109951646
SASA: 106586600
OTW: 112240651
ELS: 105016430
SFM: 108937872
LCM: 102345588
CMK: 103180179
DME: Dmel_CG7724(CG7724)
DER: 6543694
DSI: Dsimw501_GD14693(Dsim_GD14693)
DWI: 6645028
MDE: 101899660
AAG: 5566270
NVI: 100120136
CLEC: 106661338
ZNE: 110831195
FCD: 110862702
TUT: 107363285
MYI: 110457155
LAK: 106151343
MTU: Rv1106c
MTC: MT1137
MRA: MRA_1117
MTUR: CFBS_1174
MTD: UDA_1106c
MTUC: J113_07785
MTUE: J114_05980
MTUH: I917_07855
MTUL: TBHG_01091
MTUT: HKBT1_1172
MTUU: HKBT2_1178
MBB: BCG_1166c
MBT: JTY_1139
MBX: BCGT_0934
MAF: MAF_11210
MMIC: RN08_1242
MLE: ML1942
MLB: MLBr01942
MPA: MAP_2688
MAO: MAP4_1128
MAVI: RC58_05605
MAVU: RE97_05600
MAV: MAV_1225
MIT: OCO_11340
MIA: OCU_11320
MID: MIP_01824
MYO: OEM_11450
MIR: OCQ_11350
MLP: MLM_1173
MUL: MUL_0172
MMC: Mmcs_4109
MKM: Mkms_4185
MJL: Mjls_4340
MMI: MMAR_4359
MMM: W7S_05545
MHAD: B586_07090
MVA: Mvan_4635
MGI: Mflv_2075
MVQ: MYVA_4444
 » show all
Taxonomy
Reference
1  [PMID:4226148]
  Authors
Cheatum SG, Watten JC.
  Title
Purification and properties of 3-beta-hydroxysteroid dehydrogenase and delta-5-3-ketosteroid isomerase from bovine corpora lutea.
  Journal
Biochim Biophys Acta 122:1-13 (1966)
Reference
2  [PMID:14220672]
  Authors
KORITZ SB.
  Title
THE CONVERSION OF PREGNENOLONE TO PROGESTERONE BY SMALL PARTICLES FROM RAT ADRENAL.
  Journal
Biochemistry 3:1098-102 (1964)
Reference
3
  Authors
Neville, A.M., Orr, J.C. and Engel, L.L.
  Title
Delta5-3beta-Hydroxy steroid dehydrogenase activities of bovine adrenal cortex.
  Journal
Biochem J 107:20 (1968)
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.145
IUBMB Enzyme Nomenclature: 1.1.1.145
ExPASy - ENZYME nomenclature database: 1.1.1.145
BRENDA, the Enzyme Database: 1.1.1.145
CAS: 9044-85-3

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