KEGG   ENZYME: 1.1.1.261Help
Entry
EC 1.1.1.261                Enzyme                                 

Name
sn-glycerol-1-phosphate dehydrogenase;
glycerol-1-phosphate dehydrogenase [NAD(P)+];
sn-glycerol-1-phosphate:NAD+ oxidoreductase;
G-1-P dehydrogenase;
Gro1PDH;
AraM
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
sn-glycerol-1-phosphate:NAD(P)+ 2-oxidoreductase
Reaction(IUBMB)
sn-glycerol 1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H + H+ [RN:R05679 R05680]
Reaction(KEGG)
Substrate
sn-glycerol 1-phosphate [CPD:C00623];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
glycerone phosphate [CPD:C00111];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
This enzyme is found primarily as a Zn2+-dependent form in archaea but a Ni2+-dependent form has been found in Gram-positive bacteria [6]. The Zn2+-dependent metalloenzyme is responsible for the formation of archaea-specific sn-glycerol-1-phosphate, the first step in the biosynthesis of polar lipids in archaea. It is the enantiomer of sn-glycerol 3-phosphate, the form of glycerophosphate found in bacteria and eukaryotes. The other enzymes involved in the biosynthesis of polar lipids in archaea are EC 2.5.1.41 (phosphoglycerol geranylgeranyltransferase) and EC 2.5.1.42 (geranylgeranylglycerol-phosphate geranylgeranyltransferase), which together alkylate the hydroxy groups of glycerol 1-phosphate to give unsaturated archaetidic acid, which is acted upon by EC 2.7.7.67 (CDP-archaeol synthase) to form CDP-unsaturated archaeol. The final step in the pathway involves the addition of L-serine, with concomitant removal of CMP, leading to the production of unsaturated archaetidylserine [4]. Activity of the enzyme is stimulated by K+ [2].
History
EC 1.1.1.261 created 2000, modified 2009
Pathway
ec00564  Glycerophospholipid metabolism
Orthology
K00096  glycerol-1-phosphate dehydrogenase [NAD(P)+]
Genes
VHR: AL538_10680
SSE: Ssed_0112
SVO: SVI_0105(egsA)
PTU: PTUN_a1851(araM)
VAP: Vapar_4002
VPD: VAPA_1c41540
DVU: DVUA0098
DVL: Dvul_3015
DVG: Deval_3193
DGG: DGI_1035
SCL: sce5394(gldA)
WOL: WD_0787
WEN: wHa_06670
WED: wNo_03550
WPI: WP0692
WCL: WCLE_001040(egsA)
AMA: AM1109
AMF: AMF_838(egsA)
APH: APH_1193
APY: YYU_05505
APD: YYY_05575
APHA: WSQ_05560
ERU: Erum8350
ERW: ERWE_CDS_08850(egsA)
ERG: ERGA_CDS_08770(egsA)
ECN: Ecaj_0871
ECH: ECH_1084(araM)
ECHA: ECHHL_0092
ECHP: ECHWP_0090
EHH: EHF_0095
NSE: NSE_0940
NRI: NRI_0900
NHM: NHE_0897
RBT: NOVO_02695(egsA)
SMD: Smed_2701
RDE: RD1_3615
BSU: BSU28760(araM)
BSR: I33_2932
BSL: A7A1_3171
BSH: BSU6051_28760(araM)
BSUT: BSUB_03064(araM)
BSUL: BSUA_03064(araM)
BSUS: Q433_15560
BSS: BSUW23_13975(araM)
BST: GYO_3126
BSO: BSNT_09296(araM)
BSQ: B657_28760(araM)
BSX: C663_2721(egsA)
BLI: BL00250 BL00349(araM)
BLD: BLi03025(araM) BLi04259
BAY: RBAM_025820(araM)
BAQ: BACAU_2599(araM)
BYA: BANAU_2797(araM)
BAMP: B938_13345
BAML: BAM5036_2522(araM)
BAMA: RBAU_2719(araM)
BAMN: BASU_2525(araM)
BAMB: BAPNAU_0971(araM)
BAMT: AJ82_14580
BAMY: V529_28690(araM)
BMP: NG74_02722(egsA)
BAO: BAMF_2681(araM)
BAZ: BAMTA208_14130(araM)
BQL: LL3_02965(araM)
BXH: BAXH7_02894(araM)
BQY: MUS_3151(araM)
BAMI: KSO_006140
BAMC: U471_26820
BAMF: U722_14000
BATR: TD68_11190
BHA: BH1862
BCL: ABC0405(araM)
BJS: MY9_2868
BACP: SB24_15760
BACB: OY17_16215
BACY: QF06_12585
BACL: BS34A_31380(araM)
BALM: BsLM_2856
BBEV: BBEV_1649(egsA-1) BBEV_2998(egsA-2)
GTN: GTNG_1788
GGH: GHH_c19500(egsA)
AFL: Aflv_1552
PPY: PPE_01661
PPM: PPSC2_08745(araM)
PPO: PPM_1663(araM)
PPOL: X809_08995
PPQ: PPSQR21_017380(gldA)
PPOY: RE92_03560
PMW: B2K_31905
PSAB: PSAB_03670
PSWU: SY83_19995
AAC: Aaci_2896
AAD: TC41_3261(araM)
SNE: SPN23F03300(wchY)
CAC: CA_C1626
CAE: SMB_G1651
CAY: CEA_G1640
CBK: CLL_A2956
CBT: CLH_2709
CLS: CXIVA_14120(GldA)
CSR: Cspa_c43550(egsA)
CPAS: Clopa_4395
CPAT: CLPA_c35790(egsA)
CPAE: CPAST_c35790(egsA)
CSB: CLSA_c14810(egsA1) CLSA_c18080(egsA2)
CTYK: CTK_C20840
CCE: Ccel_2486
CSS: Cst_c15320(egsA1) Cst_c16390(egsA2) Cst_c16420(egsA3)
RIX: RO1_26120
RIM: ROI_37560
ROB: CK5_09860
BPRL: CL2_20430
ERT: EUR_26940
ERA: ERE_07100
AWO: Awo_c07780(egsA)
TIT: Thit_1700
CHY: CHY_2583
CSC: Csac_0948
ATE: Athe_0802
TTM: Tthe_0671
TSH: Tsac_1288
LPIL: LIP_2112
SCO: SCO2598(SCC88.09c) SCO6754(SC6A5.03)
SMA: SAVERM_1659(gldA)
SGR: SGR_971
SGB: WQO_30735
SCT: SCAT_4934
SFA: Sfla_0653
SBH: SBI_02618
SVE: SVEN_6441
SALB: XNR_0277
SALS: SLNWT_6805
STRP: F750_6216
SFI: SFUL_6524
SALU: DC74_6773
SALL: SAZ_35185
STRE: GZL_01991
SLD: T261_1212
STRM: M444_28135
SPRI: SPRI_0991
SRW: TUE45_07295(egsA)
SLE: sle_10610(sle_10610) sle_46390(sle_46390)
SRN: A4G23_05033(egsA)
SMAL: SMALA_6332
SLAU: SLA_6399
SALF: SMD44_07308(araM)
SALJ: SMD11_1107(araM)
SLX: SLAV_07435(egsA)
SFK: KY5_7128
BCV: Bcav_1503
PAW: PAZ_c15740(egsA1) PAZ_c24190(egsA2)
PFR: PFREUD_22380(araM)
PFRE: RM25_2119
ACIJ: JS278_01311(egsA_1) JS278_02177(egsA_2)
KFL: Kfla_1570
TFU: Tfu_2533
NDA: Ndas_3866
NAL: B005_1036
TCU: Tcur_4107
SRO: Sros_1355
SAQ: Sare_5002
MIL: ML5_6154
ASE: ACPL_8272(gldA)
ACTN: L083_8085(gldA)
AFS: AFR_42400
ACTS: ACWT_8141
SNA: Snas_5680
TBI: Tbis_0723
RCA: Rcas_1386
ATM: ANT_17650
PIR: VN12_10045(egsA)
PLM: Plim_1578
PLS: VT03_10495(egsA)
PLH: VT85_15600(egsA_1) VT85_15630(egsA_2)
GES: VT84_25570(egsA)
SACI: Sinac_1074
PBOR: BSF38_04241(egsA)
PGI: PG_1514
AFD: Alfi_2303
ASH: AL1_30940
TMA: TM0285
TMW: THMA_0292
TMQ: THMB_0292
TMX: THMC_0292
TPT: Tpet_0627
TRQ: TRQ2_0652
TNA: CTN_0399
TNP: Tnap_0917
TLE: Tlet_1523
PMO: Pmob_0302
DTN: DTL3_0411
DTU: Dtur_1751
MJA: MJ_0712
MMP: MMP0225(gldA)
MMD: GYY_01145
MAE: Maeo_0518
MVO: Mvol_1465
MTH: MTH_610
MMG: MTBMA_c09900(egsA)
METC: MTCT_0534
MWO: MWSIV6_0978(egsA)
METE: tca_00581(egsA)
MST: Msp_0997(egsA)
METB: AW729_00035(egsA)
MSI: Msm_0286
MRU: mru_0955(egsA)
MEB: Abm4_1208
MMIL: sm9_0774(aroB1)
MEYE: TL18_06420(egsA)
MOL: YLM1_0514
METH: MBMB1_0896(egsA)
MFC: BRM9_1064(egsA)
MFI: DSM1535_1725(egsA)
MCUB: MCBB_1176(egsA)
MFV: Mfer_0007
MKA: MK1230(gldA)
AFU: AF_1674
FPL: Ferp_2181
GAC: GACE_2186
GAH: GAH_00417
TAC: Ta1158
TVO: TVG1288826(TVG1288826)
PTO: PTO0850
FAI: FAD_0289
CDIV: CPM_0156
MER: MMINT_15070(egsA)
MEAR: Mpt1_c02750(egsA)
MARC: AR505_0626
ABI: Aboo_0536
PHO: PH1475(PH1475)
PAB: PAB1907
PFU: PF1382
PFI: PFC_06135(egsA)
PYN: PNA2_0061
PYS: Py04_1369
PYC: TQ32_09550(egsA)
TKO: TK0789
TON: TON_1047
TGA: TGAM_1402(egsA)
TSI: TSIB_0726
THE: GQS_10330(egsA)
THA: TAM4_1506
THM: CL1_1645
TLT: OCC_04917(egsA)
THS: TES1_1114
TNU: BD01_1029
TEU: TEU_11035(egsA)
TGY: X802_10770(egsA)
TPEP: A0127_01135(egsA)
TGG: A3K92_04975(egsA)
TCE: A3L02_04980(egsA)
TBS: A3L01_05960(egsA)
TSL: A3L11_10175(egsA)
TTD: A3L14_08340(egsA)
TPRF: A3L09_06795(egsA)
TRL: A3L10_00635(egsA)
TPAF: A3L08_09100(egsA)
PPAC: PAP_05350(egsA)
MAC: MA_3686(gldA)
MBAR: MSBR2_2620
MBAK: MSBR3_2805
MMA: MM_0590
MMAC: MSMAC_0720
METM: MSMTP_0906
MTHE: MSTHC_0856
MTHR: MSTHT_2426
MHOR: MSHOH_0834
MBU: Mbur_1032
MMET: MCMEM_1514
MMH: Mmah_1407
MPY: Mpsy_0964
MTP: Mthe_0219
MCJ: MCON_1056(egsA)
MHI: Mhar_0956
MHU: Mhun_1136
MLA: Mlab_1308
MBG: BN140_1475(egsA)
MEMA: MMAB1_1894(egsA)
MPI: Mpet_1786
MBN: Mboo_1960
MPL: Mpal_0948
MPD: MCP_0189(egsA)
MEZ: Mtc_0013(gldA)
RCI: RCIX1388(gldA)
HAL: VNG_0406C
HSL: OE_1602F(gldA2)
HDL: HALDL1_06185(egsA)
HHB: Hhub_1571(gldA)
HJE: HacjB3_13715(egsA)
HALH: HTSR_1341(egsA)
HHSR: HSR6_1413(egsA)
HSU: HLASF_0700(egsA)
HSF: HLASA_0697(egsA)
HMA: rrnAC0175(gldA)
HHI: HAH_0925(gldA)
HHN: HISP_04765(egsA)
HAB: SG26_12455(egsA)
NPH: NP_4492A(gldA)
NMO: Nmlp_3111(gldA)
HUT: Huta_0604
HTI: HTIA_0352
HMU: Hmuk_3139
HWA: HQ_1560A(gldA)
HWC: Hqrw_1664(gldA)
HVO: HVO_0822(gldA)
HME: HFX_0792(gldA)
HGI: ABY42_03945(egsA)
HLA: Hlac_0655
HTU: Htur_3191
NMG: Nmag_2535(gldA1)
NAT: NJ7G_2596
SALI: L593_01290(egsA)
HLR: HALLA_16230(egsA)
ACJ: ACAM_0400
SMR: Smar_0939
IHO: Igni_1174
IIS: EYM_00840(egsA)
DKA: DKAM_1289
TAG: Tagg_1251
IAG: Igag_0044
HBU: Hbut_1566
STO: STK_03440
SSO: SSO0760(gldA)
SOL: Ssol_1816
SSOA: SULA_1845
SSOL: SULB_1846
SSOF: SULC_1844
SAI: Saci_0640
SACN: SacN8_03105(egsA)
SACR: SacRon12I_03095(egsA)
SACS: SUSAZ_02875(egsA)
SID: M164_1374
SII: LD85_1586
SIH: SiH_1327
SIR: SiRe_1249
SIC: SiL_1240
MSE: Msed_2255
MCN: Mcup_2002
AHO: Ahos_0639
PAI: PAE1685
PIS: Pisl_1689
PCL: Pcal_0566
PAS: Pars_1827
PYR: P186_0043
POG: Pogu_0303
TNE: Tneu_0711
CMA: Cmaq_1971
TUZ: TUZN_2018
TTN: TTX_1518(gldA)
VMO: VMUT_0721
TPE: Tpen_1231
ASC: ASAC_0315
ACIA: SE86_02375
NMR: Nmar_1729
NID: NPIRD3C_0179(egsA)
NIN: NADRNF5_0142(egsA)
NCT: NMSP_1647(egsA_2)
NGA: Ngar_c23480(egsA)
NVN: NVIE_000470(egsA)
NEV: NTE_01413
TAA: NMY3_01912(egsA)
NCV: NCAV_0598
NBV: T478_1440
NDV: NDEV_2036(egsA)
KCR: Kcr_0068
BARB: AOA66_1645(egsA)
 » show all
Taxonomy
Reference
1  [PMID:8586635]
  Authors
Nishihara M, Koga Y
  Title
sn-glycerol-1-phosphate dehydrogenase in Methanobacterium thermoautotrophicum: key enzyme in biosynthesis of the enantiomeric glycerophosphate backbone of ether phospholipids of archaebacteria.
  Journal
J Biochem 117:933-5 (1995)
Reference
2  [PMID:9348086]
  Authors
Nishihara M, Koga Y
  Title
Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea.
  Journal
J Biochem 122:572-6 (1997)
  Sequence
[mth:MTH_610]
Reference
3  [PMID:9419225]
  Authors
Koga Y, Kyuragi T, Nishihara M, Sone N.
  Title
Did archaeal and bacterial cells arise independently from noncellular precursors? A hypothesis stating that the advent of membrane phospholipid with enantiomeric glycerophosphate backbones caused the separation of the two lines of descent.
  Journal
J Mol Evol 46:54-63 (1998)
  Sequence
[mth:MTH_610]
Reference
4  [PMID:10960477]
  Authors
Morii H, Nishihara M, Koga Y
  Title
CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase in the methanogenic archaeon Methanothermobacter thermoautotrophicus.
  Journal
J Biol Chem 275:36568-74 (2000)
DOI:10.1074/jbc.M005925200
Reference
5  [PMID:15876564]
  Authors
Han JS, Ishikawa K
  Title
Active site of Zn(2+)-dependent sn-glycerol-1-phosphate dehydrogenase from Aeropyrum pernix K1.
  Journal
Archaea 1:311-7 (2005)
  Sequence
Reference
6  [PMID:18558723]
  Authors
Guldan H, Sterner R, Babinger P
  Title
Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni(2+)-dependent AraM from Bacillus subtilis.
  Journal
Biochemistry 47:7376-84 (2008)
DOI:10.1021/bi8005779
  Sequence
[bsu:BSU28760]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.261
IUBMB Enzyme Nomenclature: 1.1.1.261
ExPASy - ENZYME nomenclature database: 1.1.1.261
BRENDA, the Enzyme Database: 1.1.1.261
CAS: 204594-18-3

DBGET integrated database retrieval system